JPS59225121A - インタ−ロイキン２の精製濃縮法 - Google Patents
インタ−ロイキン２の精製濃縮法Info
- Publication number
- JPS59225121A JPS59225121A JP58099039A JP9903983A JPS59225121A JP S59225121 A JPS59225121 A JP S59225121A JP 58099039 A JP58099039 A JP 58099039A JP 9903983 A JP9903983 A JP 9903983A JP S59225121 A JPS59225121 A JP S59225121A
- Authority
- JP
- Japan
- Prior art keywords
- carrier
- carriers
- chromatography
- buffer
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
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- LFGREXWGYUGZLY-UHFFFAOYSA-N phosphoryl Chemical group [P]=O LFGREXWGYUGZLY-UHFFFAOYSA-N 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000012475 sodium chloride buffer Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000000547 substituted alkyl group Chemical group 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 125000000542 sulfonic acid group Chemical group 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- AKYUZBFQLWFNOB-UHFFFAOYSA-K trisodium 2-hydroxypropane-1,2,3-tricarboxylate hydrochloride Chemical compound [Na+].[Na+].[Na+].Cl.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O AKYUZBFQLWFNOB-UHFFFAOYSA-K 0.000 description 1
- LEAHFJQFYSDGGP-UHFFFAOYSA-K trisodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[Na+].OP(O)([O-])=O.OP([O-])([O-])=O LEAHFJQFYSDGGP-UHFFFAOYSA-K 0.000 description 1
- 229910052722 tritium Inorganic materials 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58099039A JPS59225121A (ja) | 1983-06-03 | 1983-06-03 | インタ−ロイキン２の精製濃縮法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58099039A JPS59225121A (ja) | 1983-06-03 | 1983-06-03 | インタ−ロイキン２の精製濃縮法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59225121A true JPS59225121A (ja) | 1984-12-18 |
| JPH0478639B2 JPH0478639B2 (enrdf_load_stackoverflow) | 1992-12-11 |
Family
ID=14236301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58099039A Granted JPS59225121A (ja) | 1983-06-03 | 1983-06-03 | インタ−ロイキン２の精製濃縮法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59225121A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994003626A1 (en) * | 1992-07-31 | 1994-02-17 | The Green Cross Corporation | Method of highly purifying human serum albumin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55150900A (en) * | 1979-05-10 | 1980-11-25 | Toray Ind Inc | Production of interferon |
| JPS58190397A (ja) * | 1982-04-20 | 1983-11-07 | スロ−ン−ケツタリング・インステイテユ−ト・フオ−・キヤンサ−・リサ−チ | インターリューキン−２およびその純化方法 |
-
1983
- 1983-06-03 JP JP58099039A patent/JPS59225121A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55150900A (en) * | 1979-05-10 | 1980-11-25 | Toray Ind Inc | Production of interferon |
| JPS58190397A (ja) * | 1982-04-20 | 1983-11-07 | スロ−ン−ケツタリング・インステイテユ−ト・フオ−・キヤンサ−・リサ−チ | インターリューキン−２およびその純化方法 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994003626A1 (en) * | 1992-07-31 | 1994-02-17 | The Green Cross Corporation | Method of highly purifying human serum albumin |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0478639B2 (enrdf_load_stackoverflow) | 1992-12-11 |
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