JPS5841352A - Automatic fraction collector - Google Patents

Abstract

PURPOSE:To achieve a fractional collection of intended components regardless of changes in the base line by fractional collection thereof as set peak fraction with a decision on the peak thereof detecting differential variations in the rise at the set fraction peak. CONSTITUTION:When receiving an injection marker signal indicating the start, a peak detector 3 detects the peak by setting a peak sensitivity. An LPE1 prevents spike noises or the like. The marker signal is amplified with an amplifier 5 to serve as input signal for a time setting device 2 and triggers the setting device 2. The setting device 2 is latched after the elapsing of a set time and then, when a peak set for fraction in a liquid chromatograph is detected, it is ANDed with a set time signal latched to output pulses for starting fractional collection. Therefore, no detection is performed how much the base line varies within the set time and if the time is set with a enough room initially until the base is stabilized, the detection of the fraction peak can be done accurately.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a frame and a liquid talomagraph [an automatic fractionating device used].

In recent years, there has been a demand for equipment that uses liquid chromatographs to repeatedly fractionate and isolate trace amounts of physiologically active substances over 10 times, but it is difficult to carry out the separation of target components with the current equipment. A method of self-separation based on the passage of time from the start signal, or vs is a certain threshold level! There is a way to set the level ([1 beater judgment when used) and automatically separate the sample.

However, in the former case, it is necessary to always keep the generation time of each preparative beater of the chromatograph constant.

If the retention time deviates, the target component cannot be fractionated. There are cases where too much is left behind.

There was a drawback that it was not possible to take a 9m 9m distance.

Therefore, the present @ is the 4o made by O fault #C Kanmoto, and 1. In the preparative device, it is necessary to smooth the initial y representing the start y, and the preparative settings. It consists of a time setting device that masters as the cine main band by the tell setting until the peak rising signal, and a peak detector that detects the increase in the preparative beak O rising 9 minute change. It is intended to provide an apparatus tII that detects t and performs aliquoting for the purpose regardless of retention time deviations or baseline changes.

Hereinafter, one implementation of the present invention will be described in detail. Block 1111 showing the detection configuration of the frame beater - Island 9.
start! Indicator marker signal 111
00~500111B, 1117 level is common, and when the signal is received, the peak detector 3RI performs beater detection by setting the peak sensitivity, so 2~zonzo low pass filter! -1 ram to prevent spyter noise, etc.!
I'm angry because I bought the protective measures that were included. Also, in order to make it also serve as the input signal for the HaV marker signal time setter 20, it is amplified by the amplification 65 and sent to the time setter 2? Let it be rigged.

When the time setter 2ri is triggered, the time setter 2ri is latched after the set time has elapsed, and then when the decentralization setting beater of the liquid chromatograph is detected, the latched set time signal is set, and the separation is started. Output to pulse. Therefore,
No matter how much the baseline fluctuates within the set time, one detection operation will not be performed, so preparative peak O detection can be performed reliably by setting a margin of time for the first baseline to stabilize. is possible.

Showing the Mayme chart of ZSa fractional treatment, how to detect the beater set in 纂3-! This is the explanation shown, Part 3
mK'lk, first set the detection sensitivity of the rising peak, and then set the detection sensitivity of Taromato Darafu r constantly.
The differential value P is compared with the previous differential value P-1, and the compared differential value P is thicker than the differential value P-1, and the peak rises far! jA#C enters, but when it is determined that the detection sensitivity is larger than the initially set detection sensitivity, that is, P-CP
-1) > Peak detection f#i when the detection sensitivity setting value is different
The signal is output, the solenoid valve 8 (see collection 1-) is excited, and the component of the set peak is fractionated.

As explained above in detail 1@C, the time period from when the preparative separation M device of the present invention receives the injector marker signal indicating the start until the preparative beater that has been set arrives.

The detection of the peak is not affected by the preceding fluctuation peak and is positive.
I in C! , preparative collection of set peaks, column switching, etc.
A high Hf, low cost preparative separation device that can be automated in one step! Can be provided.

[Brief explanation of the drawing]

Block 1 shows peak detection configuration according to the present invention.
How to detect peaks with 3tlQH settings! FIG. l... Filter, 2. One hour setting device. 3.-beater detector, peak setting device, 5.-amplifier, 6.-electromagnetic valve. Applicant: Ajinomoto Co., Ltd. Attorney: Hideaki Sato

Claims (1)

[Claims] Liquid Taromatodara 7! I used a preparative separation device I for free time.
cy, A7 extraction marker signal representing star 11 [a time setting device that masters the interval from the smoothing filter to the 1 preparative preparative setting Peter O rise signal as a dead zone by setting the time, and the preparative peak that has been set. An automatic preparative separation device called 'fl#*, which consists of a peak detector that detects the rising owl and as content.