JPS5719666A - Fractionation treating method in electrophoresis - Google Patents

Fractionation treating method in electrophoresis

Info

Publication number
JPS5719666A
JPS5719666A JP9416180A JP9416180A JPS5719666A JP S5719666 A JPS5719666 A JP S5719666A JP 9416180 A JP9416180 A JP 9416180A JP 9416180 A JP9416180 A JP 9416180A JP S5719666 A JPS5719666 A JP S5719666A
Authority
JP
Japan
Prior art keywords
serum
fractionation
points
standard
globulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9416180A
Other languages
Japanese (ja)
Other versions
JPS6259772B2 (en
Inventor
Toshihide Fujiwara
Shinichi Gamachi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Optical Co Ltd filed Critical Olympus Optical Co Ltd
Priority to JP9416180A priority Critical patent/JPS5719666A/en
Priority to US06/281,495 priority patent/US4420383A/en
Priority to DE3127007A priority patent/DE3127007C2/en
Publication of JPS5719666A publication Critical patent/JPS5719666A/en
Publication of JPS6259772B2 publication Critical patent/JPS6259772B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To fractionate automatically and accurately using a computer, by taking minimum values of an electrophoretic pattern of a standard serum as reference points, comparing said values with minimum values of an electrophoretic pattern of a specimen and taking the closest minimum value among them as a fractionation point. CONSTITUTION:In the determination of five fractions of albumin, alpha1 globulin, alpha2 globulin, beta globulin and gamma globulin by means of electrophesis of a human serum using a cellulose acetate film, in order that minimum values b1-b4 of a pattern of a standard serum are taken as reference and compared with a pattern of a specimen to thereby remove unnecessary minimum points b5', b6' generated by turbidity and freshness of the specimen from fractionation points, the calculation is performed by placing peak ao of a standard serum and peak ao' of a serum to be tested at the same point on an X axis of coordinates, and by taking advantage of the fact that ratios of distances from peak ao to the minumim points b1-b4 on the X axis are the same for the serum of a patient suffering from an immune disease and the standard serum, to thereby take b' closest to a standard ratio as a fractionation point and remove b5' and b6. Thus, by using a computer, it is posible to perform a fractionation treatment without collecting a standard serum every time.
JP9416180A 1980-07-10 1980-07-10 Fractionation treating method in electrophoresis Granted JPS5719666A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP9416180A JPS5719666A (en) 1980-07-10 1980-07-10 Fractionation treating method in electrophoresis
US06/281,495 US4420383A (en) 1980-07-10 1981-07-08 Fractionating method in electrophoreses
DE3127007A DE3127007C2 (en) 1980-07-10 1981-07-09 Method for determining the reference points of a densitogram

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9416180A JPS5719666A (en) 1980-07-10 1980-07-10 Fractionation treating method in electrophoresis

Publications (2)

Publication Number Publication Date
JPS5719666A true JPS5719666A (en) 1982-02-01
JPS6259772B2 JPS6259772B2 (en) 1987-12-12

Family

ID=14102640

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9416180A Granted JPS5719666A (en) 1980-07-10 1980-07-10 Fractionation treating method in electrophoresis

Country Status (1)

Country Link
JP (1) JPS5719666A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0514928A2 (en) * 1991-05-24 1992-11-25 Wakamoto Pharmaceutical Co., Ltd. Method of diagnosing renal diseases by detecting albumin fragments
JP2009204465A (en) * 2008-02-28 2009-09-10 Jokoh Co Ltd Method for determining pathology of kidney disease or like by electrophoretic method using silver staining

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3130629B2 (en) * 1992-03-13 2001-01-31 オリンパス光学工業株式会社 Fractionation treatment method in electrophoresis

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0514928A2 (en) * 1991-05-24 1992-11-25 Wakamoto Pharmaceutical Co., Ltd. Method of diagnosing renal diseases by detecting albumin fragments
JP2009204465A (en) * 2008-02-28 2009-09-10 Jokoh Co Ltd Method for determining pathology of kidney disease or like by electrophoretic method using silver staining

Also Published As

Publication number Publication date
JPS6259772B2 (en) 1987-12-12

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