JPS5719650A - Split processing method in electrophoresis - Google Patents

Split processing method in electrophoresis

Info

Publication number
JPS5719650A
JPS5719650A JP9416280A JP9416280A JPS5719650A JP S5719650 A JPS5719650 A JP S5719650A JP 9416280 A JP9416280 A JP 9416280A JP 9416280 A JP9416280 A JP 9416280A JP S5719650 A JPS5719650 A JP S5719650A
Authority
JP
Japan
Prior art keywords
split
globulin
case
pattern
ratio
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9416280A
Other languages
Japanese (ja)
Other versions
JPS6321860B2 (en
Inventor
Toshihide Fujiwara
Shinichi Gamachi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Optical Co Ltd filed Critical Olympus Optical Co Ltd
Priority to JP9416280A priority Critical patent/JPS5719650A/en
Priority to US06/281,495 priority patent/US4420383A/en
Priority to DE3127007A priority patent/DE3127007C2/en
Publication of JPS5719650A publication Critical patent/JPS5719650A/en
Publication of JPS6321860B2 publication Critical patent/JPS6321860B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To enable finding of a correct split point without taking a reference sample from a normal person at every time, by a method wherein a ratio of a distance between peak values or lowest values of each pattern of split images of the sample to be measured is compared with the corresponding ratio of a normal person. CONSTITUTION:A spacing ratio between peaks a0, a1-a4 and lowest values b1- b4 on an X-axis of each split pattern of albumin, alpha1, globulin, alpha2 globulin, beta globulin, and tau globulin in a serum, which are obtained by a electrophoresis method, does not change both in the case of the normal person and in the case of the immune patient. Through the harnessing of said phenomenon, in case a peack of a matter (beta lipo protein and the like) except said 5 components is created at an inspected serum pattern, and in case there are, for example, very low values b5, b6, b7, b8 in addition to b1-b4, the very low value b2, among the b5, b2, b6 between the peaks a1-a2, in which said spacing ratio is most nearly equal to the reference value, is determined as a split point, and the b7 and b8 are erased by counting as described above. This enables them to be processed by means of a computer to correctly measure an area of each split and to diagonize the presence of the abnormality.
JP9416280A 1980-07-10 1980-07-10 Split processing method in electrophoresis Granted JPS5719650A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP9416280A JPS5719650A (en) 1980-07-10 1980-07-10 Split processing method in electrophoresis
US06/281,495 US4420383A (en) 1980-07-10 1981-07-08 Fractionating method in electrophoreses
DE3127007A DE3127007C2 (en) 1980-07-10 1981-07-09 Method for determining the reference points of a densitogram

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9416280A JPS5719650A (en) 1980-07-10 1980-07-10 Split processing method in electrophoresis

Publications (2)

Publication Number Publication Date
JPS5719650A true JPS5719650A (en) 1982-02-01
JPS6321860B2 JPS6321860B2 (en) 1988-05-09

Family

ID=14102667

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9416280A Granted JPS5719650A (en) 1980-07-10 1980-07-10 Split processing method in electrophoresis

Country Status (1)

Country Link
JP (1) JPS5719650A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3130629B2 (en) * 1992-03-13 2001-01-31 オリンパス光学工業株式会社 Fractionation treatment method in electrophoresis

Also Published As

Publication number Publication date
JPS6321860B2 (en) 1988-05-09

Similar Documents

Publication Publication Date Title
Awdeh et al. Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins
Bini et al. Two‐dimensional electrophoretic patterns of acute‐phase human serum proteins in the course of bacterial and viral diseases
ATE59902T1 (en) OPTOELECTRONIC POSITION SENSING.
IT8023609A0 (en) COMPOSITIONS SUITABLE FOR SEPARATING RED BLOOD BLOODS FROM SERUM OR PLASMA IN BLOOD SAMPLES FOR ANALYSIS AND THE METHOD THAT USES THEM.
Herrero‐Martínez et al. Determination of cow's milk and ripening time in nonbovine cheese by capillary electrophoresis of the ethanol‐water protein fraction
JPS5719650A (en) Split processing method in electrophoresis
EP0259183A3 (en) Process for controlling the accuracy and precision of sensitivity assays
EP0125893A3 (en) The quantitative analysis of antigen by the enzyme-antibody bridge method
EP0347645A3 (en) Monitoring a plurality of parameters from identical processes
JPS57118639A (en) Process control of semiconductor photo-etching
JPS5719666A (en) Fractionation treating method in electrophoresis
Pattison et al. Elution patterns of rubella IgM, IgA, and IgG antibodies from a dextran and an agarose gel.
Hillman Computer analysis of gamma and X-ray spectra
Chang et al. The purification of human chorionic gonadotropin on DEAE-Sephadex
Remington et al. Changes in serum proteins of rats infected with Toxoplasma gondii
JPS533893A (en) Constant speed analyzer by electric pulsation
Kjellin et al. Cerebrospinal fluid proteins in infectious neurological diseases and Guillain-Barré syndrome
Brown Application of automation and computerisation to a soil testing laboratory
Glenn Some considerations in agar column diffusion analyses
Antolković Correlation measurements of neutron-induced multiparticle reactions in nuclear emulsions
Peele Jr et al. Combined gel filtration, biuret/copper method compared with an immunochemical method for urinary protein measurement.
Farstad Determination of total lipids and the lipids of lipoprotein fractions of serum
Bienvenu et al. Enzyme-linked immunoadsorbent assay for serum IgE: evaluation in four centres
MacDonald et al. Comparison of Serum Thyroxine, Protein-Bound Iodine and Thyroxine Iodine Methods
JPS6126848A (en) Two-dimensional electrophoresis device