JPH05256839A - Chromatograph - Google Patents

Abstract

PURPOSE:To obtain a liquid chromatograph by which it can be easily confirmed that determination is surely performed. CONSTITUTION:The title liquid chromatograph constituted of a pump 2, sample injector 3, column 4, detector 5, and data processor 6 collectively outputs determined results obtained by means of a chromatogram waveform processing or calibration curve preparing function as one output. Or, the chromatograph compares the determined results obtained by means of the chromatogram waveform processing or calibration curve preparing function with each other and, when the difference between them exceeds a preset range, outputs the determined results together with a marker indicating the difference. Therefore, the completion of determination can be surely confirmed.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid chromatograph, and more particularly to a liquid chromatograph suitable for confirming whether or not quantification has been performed correctly.

[0002]

2. Description of the Related Art In a liquid chromatograph, a predetermined chromatogram waveform processing or a calibration curve creating function is used to perform quantification and output the result. Some have a plurality of calibration curve creation functions and a function to perform them continuously, but the outputs were made separately.

[0003]

The above-mentioned prior art does not consider using different chromatogram waveform processing or a calibration curve creating function as a material for judging that the quantification was performed correctly. There was a problem that it was necessary to check again after the output was output.

An object of the present invention is to provide a liquid chromatograph in which it is easy to confirm that quantification has been performed reliably.

[0005]

For the above purpose, the quantitative results obtained by different chromatogram waveform processing or calibration curve creating functions are collectively output as one output. Alternatively, the quantification results obtained by different chromatogram waveform processing or calibration curve creation functions were compared, and if they differ by more than a preset range, a marker indicating that is output simultaneously in the quantification results.

[0006]

[Function] In quantification by liquid chromatography,
When each component in the sample is completely separated, the quantification result does not significantly change due to the chromatogram waveform processing or the calibration curve creating function. However, if the separation is incomplete, the quantitative value may differ due to the difference in the waveform processing method for creating the baseline or the calibration curve creating function such as area and height. By collectively outputting the quantitative results obtained by different chromatogram waveform processing or calibration curve creation functions to one output, it is possible to easily determine how much the quantitative values differ due to these differences and ensure identification. It can be confirmed that it was done in.

[0007]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to FIGS. FIG. 1 shows an example of the system configuration, in which 1 is an eluent and 2 is a pump. The sample is injected from the sample injector of 3 and separated by the column of 4. 5
The detector of (1) converts the change of the eluate from the column into an electric signal, which is sent to the data processing unit (6) for identification and quantification. FIG. 2 is an example of the quantitative result output. The peak number, the retention time, the component name, and the peak area are output together with the quantitative value 1 determined by the peak area and the quantitative value 2 determined by the peak height. FIG. 3 shows another example of the quantitative result output. Quantitative values obtained by quantification by peak number, retention time, component name, peak area and peak area are output. In addition to these,
When the quantitative values obtained by different waveform processing methods differ by 10% or more, a marker (*) is output after the quantitative value. Here, for the output peak area and the peak area used for the output quantitative value calculation, if multiple peaks are not completely separated, reading, vertical division, and tailing processes are automatically performed according to the separation shape. However, the peak area when vertically dividing all unseparated peaks is calculated at the same time.
It is compared with the peak area of the above calculation method. Then, when the calculated values of the both differ by 10% or more, a marker is output. FIG. 4 shows another configuration example of the system,
1 is an eluent, 2 is a pump, 3 is a sample injector,
4 is a column and 5 is a detector. The data processing device 6 is connected with the indicator 7 and can be turned on by the output of 6. In this data processing device, after the analysis of the actual sample is completed, the quantitative value obtained by the quantitative determination by the peak area and the quantitative value obtained by the quantitative determination by the peak height are compared, and these are equal to or more than a set value, for example, 15 If the difference is more than%, the output is changed. Here, the contact to which the indicator is connected is short-circuited and the indicator is lit to indicate that the certainty value is low in certainty. Further, when an autosampler for automatically injecting a sample is used, it is possible to prompt injection of the same sample again by using this contact signal. FIG. 5 shows an example of the progress of analysis in the case where there is one standard sample on one inspection curve. When analyzing a standard sample, a calibration curve 1 and a calibration curve 2 are prepared according to the peak area and the peak height, respectively. In actual sample analysis, quantitative values 1 and 2 are obtained from these two calibration curves. After the quantitative calculation result is output, the quantitative value 1 and the quantitative value 2 are compared, and if the difference is less than or equal to the set value, the analysis is continued, but if the difference is greater than or equal to the set value, an output indicating abnormal quantitative value is output. After doing so, proceed to the next analysis.

[0008]

EFFECTS OF THE INVENTION Because the quantitative results obtained by the different chromatogram waveform processing or the calibration curve creating function are collectively output to one output, or the quantitative results obtained by the different chromatogram waveform processing or the calibration curve creating function are provided. When the result is different from the set value or more, the marker is output, so that it is possible to easily confirm that the quantification is performed reliably.

[Brief description of drawings]

FIG. 1 is a diagram illustrating a configuration example of a system according to an embodiment.

FIG. 2 is a diagram showing an example of a quantification result output.

FIG. 3 is a diagram showing another example of quantitative result output.

FIG. 4 is a diagram showing another configuration example of the system.

FIG. 5 is a diagram showing an example of progress of analysis.

[Explanation of symbols]

1 ... Eluent, 2 ... Pump, 3 ... Sample injector,
4 ... column, 5 ... detector, 6 ... data processing device, 7 ... indicator.

Claims (6)

[Claims] 1. A sample injector, a column, a detector,
In a chromatograph consisting of a data processing device, etc., it has the function of setting multiple chromatogram waveform processing conditions or multiple calibration curve creation conditions, and the quantitative results obtained using different waveform processing methods or calibration curve creation methods A chromatograph having a function of outputting. 2. The chromatograph according to claim 1, wherein
A chromatograph, which has a function of displaying an abnormality when a quantification result obtained by using a different waveform processing method or calibration curve creation method exceeds a preset value. 3. The chromatograph according to claim 1, wherein
A chromatograph having a function of outputting a signal when a quantification result obtained by using a different waveform processing method or calibration curve creation method exceeds a preset value. 4. A data processing device having a function of performing the output according to claim 1 in a chromatograph. 5. The chromatograph according to claim 1, wherein
A data processing device having a function of performing display according to claim 2. 6. The chromatograph according to claim 1, wherein
A data processing device having a function of outputting a signal according to claim 3.