JPS58175496A - 基質または酵素活性の定量方法 - Google Patents
基質または酵素活性の定量方法Info
- Publication number
- JPS58175496A JPS58175496A JP5928582A JP5928582A JPS58175496A JP S58175496 A JPS58175496 A JP S58175496A JP 5928582 A JP5928582 A JP 5928582A JP 5928582 A JP5928582 A JP 5928582A JP S58175496 A JPS58175496 A JP S58175496A
- Authority
- JP
- Japan
- Prior art keywords
- group
- test solution
- substrate
- solution containing
- carbon atoms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 32
- 238000004445 quantitative analysis Methods 0.000 title abstract description 3
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 31
- 150000002989 phenols Chemical class 0.000 claims abstract description 25
- 102000003992 Peroxidases Human genes 0.000 claims abstract description 17
- 108040007629 peroxidase activity proteins Proteins 0.000 claims abstract description 17
- 239000000126 substance Substances 0.000 claims abstract description 13
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 10
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 10
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 9
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 8
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract description 7
- 125000002252 acyl group Chemical group 0.000 claims abstract description 6
- 229910052736 halogen Inorganic materials 0.000 claims abstract description 6
- 125000005011 alkyl ether group Chemical group 0.000 claims abstract description 5
- 150000002367 halogens Chemical class 0.000 claims abstract description 5
- 125000004453 alkoxycarbonyl group Chemical group 0.000 claims abstract description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 3
- 239000001257 hydrogen Substances 0.000 claims abstract description 3
- 239000012085 test solution Substances 0.000 claims description 71
- 102000004190 Enzymes Human genes 0.000 claims description 30
- 108090000790 Enzymes Proteins 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 29
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 20
- 125000004432 carbon atom Chemical group C* 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- 230000001590 oxidative effect Effects 0.000 claims description 8
- 125000000542 sulfonic acid group Chemical group 0.000 claims description 3
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 14
- 235000012000 cholesterol Nutrition 0.000 abstract description 7
- 238000005259 measurement Methods 0.000 abstract description 5
- 229910052799 carbon Inorganic materials 0.000 abstract description 4
- 150000002431 hydrogen Chemical class 0.000 abstract description 2
- 150000001721 carbon Chemical group 0.000 abstract 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 66
- 229940088598 enzyme Drugs 0.000 description 27
- 235000011187 glycerol Nutrition 0.000 description 27
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 20
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 12
- 239000000872 buffer Substances 0.000 description 10
- 101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 description 8
- 210000001124 body fluid Anatomy 0.000 description 7
- 239000010839 body fluid Substances 0.000 description 7
- -1 p-oxyacetylphenol Chemical compound 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- CFKMVGJGLGKFKI-UHFFFAOYSA-N 4-chloro-m-cresol Chemical compound CC1=CC(O)=CC=C1Cl CFKMVGJGLGKFKI-UHFFFAOYSA-N 0.000 description 6
- 102000057621 Glycerol kinases Human genes 0.000 description 6
- 108090001060 Lipase Proteins 0.000 description 6
- 102000004882 Lipase Human genes 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 6
- 150000001840 cholesterol esters Chemical class 0.000 description 6
- IWDCLRJOBJJRNH-UHFFFAOYSA-N p-cresol Chemical compound CC1=CC=C(O)C=C1 IWDCLRJOBJJRNH-UHFFFAOYSA-N 0.000 description 6
- 229940107700 pyruvic acid Drugs 0.000 description 6
- 239000004367 Lipase Substances 0.000 description 5
- 150000001448 anilines Chemical class 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 235000019421 lipase Nutrition 0.000 description 5
- 150000003626 triacylglycerols Chemical class 0.000 description 5
- SMFFZOQLHYIRDA-UHFFFAOYSA-N 3,4-dimethoxyphenol Chemical compound COC1=CC=C(O)C=C1OC SMFFZOQLHYIRDA-UHFFFAOYSA-N 0.000 description 4
- YQUQWHNMBPIWGK-UHFFFAOYSA-N 4-isopropylphenol Chemical compound CC(C)C1=CC=C(O)C=C1 YQUQWHNMBPIWGK-UHFFFAOYSA-N 0.000 description 4
- 239000004382 Amylase Substances 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 108010065511 Amylases Proteins 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 description 4
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 description 4
- 101710163410 Probable glycerol kinase Proteins 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000009833 condensation Methods 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 150000004780 naphthols Chemical class 0.000 description 3
- RARSHUDCJQSEFJ-UHFFFAOYSA-N p-Hydroxypropiophenone Chemical compound CCC(=O)C1=CC=C(O)C=C1 RARSHUDCJQSEFJ-UHFFFAOYSA-N 0.000 description 3
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 3
- UADYGGYNNBDDQR-UHFFFAOYSA-N (4-hydroxyphenyl)methyl hydrogen sulfate Chemical compound OC1=CC=C(COS(O)(=O)=O)C=C1 UADYGGYNNBDDQR-UHFFFAOYSA-N 0.000 description 2
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 description 2
- LUJMEECXHPYQOF-UHFFFAOYSA-N 3-hydroxyacetophenone Chemical compound CC(=O)C1=CC=CC(O)=C1 LUJMEECXHPYQOF-UHFFFAOYSA-N 0.000 description 2
- HXDOZKJGKXYMEW-UHFFFAOYSA-N 4-ethylphenol Chemical compound CCC1=CC=C(O)C=C1 HXDOZKJGKXYMEW-UHFFFAOYSA-N 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 108700016170 Glycerol kinases Proteins 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 2
- 108010055297 Sterol Esterase Proteins 0.000 description 2
- 102000000019 Sterol Esterase Human genes 0.000 description 2
- 102000003929 Transaminases Human genes 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- XSIFPSYPOVKYCO-UHFFFAOYSA-N butyl benzoate Chemical compound CCCCOC(=O)C1=CC=CC=C1 XSIFPSYPOVKYCO-UHFFFAOYSA-N 0.000 description 2
- 238000006482 condensation reaction Methods 0.000 description 2
- CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 description 2
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 2
- GGSUCNLOZRCGPQ-UHFFFAOYSA-N diethylaniline Chemical compound CCN(CC)C1=CC=CC=C1 GGSUCNLOZRCGPQ-UHFFFAOYSA-N 0.000 description 2
- 229930195712 glutamate Natural products 0.000 description 2
- 108010090622 glycerol oxidase Proteins 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- BVJSUAQZOZWCKN-UHFFFAOYSA-N p-hydroxybenzyl alcohol Chemical compound OCC1=CC=C(O)C=C1 BVJSUAQZOZWCKN-UHFFFAOYSA-N 0.000 description 2
- NWVVVBRKAWDGAB-UHFFFAOYSA-N p-methoxyphenol Chemical compound COC1=CC=C(O)C=C1 NWVVVBRKAWDGAB-UHFFFAOYSA-N 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 150000004992 toluidines Chemical class 0.000 description 2
- YJNYFHXIRJVPGM-UHFFFAOYSA-N 1-(2-chloro-5-hydroxyphenyl)ethanone Chemical compound CC(=O)C1=CC(O)=CC=C1Cl YJNYFHXIRJVPGM-UHFFFAOYSA-N 0.000 description 1
- HUXBPJVVEDIIEO-UHFFFAOYSA-N 1-(4-hydroxyphenyl)-2-methylpropan-1-one Chemical compound CC(C)C(=O)C1=CC=C(O)C=C1 HUXBPJVVEDIIEO-UHFFFAOYSA-N 0.000 description 1
- HFZWRUODUSTPEG-UHFFFAOYSA-N 2,4-dichlorophenol Chemical compound OC1=CC=C(Cl)C=C1Cl HFZWRUODUSTPEG-UHFFFAOYSA-N 0.000 description 1
- YCCILVSKPBXVIP-UHFFFAOYSA-N 2-(4-hydroxyphenyl)ethanol Chemical compound OCCC1=CC=C(O)C=C1 YCCILVSKPBXVIP-UHFFFAOYSA-N 0.000 description 1
- KRNUKKZDGDAWBF-UHFFFAOYSA-N 2-(n-ethyl-n-m-toluidino)ethanol Chemical compound OCCN(CC)C1=CC=CC(C)=C1 KRNUKKZDGDAWBF-UHFFFAOYSA-N 0.000 description 1
- CRBJBYGJVIBWIY-UHFFFAOYSA-N 2-isopropylphenol Chemical compound CC(C)C1=CC=CC=C1O CRBJBYGJVIBWIY-UHFFFAOYSA-N 0.000 description 1
- ONEJZQZXBBBCOD-UHFFFAOYSA-N 3-(5-ethyl-2-hydroxyphenyl)propane-1-sulfonic acid Chemical compound CCC1=CC=C(O)C(CCCS(O)(=O)=O)=C1 ONEJZQZXBBBCOD-UHFFFAOYSA-N 0.000 description 1
- XBPRAIIIFOIDQE-UHFFFAOYSA-N 3-(hydroxymethyl)-4-methylphenol Chemical compound CC1=CC=C(O)C=C1CO XBPRAIIIFOIDQE-UHFFFAOYSA-N 0.000 description 1
- BTIDJAQNJLWPTI-UHFFFAOYSA-N 3-(n-ethyl-3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC(OC)=CC(OC)=C1 BTIDJAQNJLWPTI-UHFFFAOYSA-N 0.000 description 1
- NZAVBNVWEPQSBL-UHFFFAOYSA-N 3-(n-ethyl-3,5-dimethoxyanilino)propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN(CC)C1=CC(OC)=CC(OC)=C1 NZAVBNVWEPQSBL-UHFFFAOYSA-N 0.000 description 1
- STBWJPWQQLXSCK-UHFFFAOYSA-N 3-(n-ethyl-3-methoxyanilino)propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN(CC)C1=CC=CC(OC)=C1 STBWJPWQQLXSCK-UHFFFAOYSA-N 0.000 description 1
- IBSUMVZKDLDAEK-UHFFFAOYSA-N 3-(n-ethyl-3-methylanilino)propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN(CC)C1=CC=CC(C)=C1 IBSUMVZKDLDAEK-UHFFFAOYSA-N 0.000 description 1
- VFEYHMUPKXHRDK-UHFFFAOYSA-N 3-butyl-4-chlorophenol Chemical compound CCCCC1=CC(O)=CC=C1Cl VFEYHMUPKXHRDK-UHFFFAOYSA-N 0.000 description 1
- BDQASBWZSSWBCP-UHFFFAOYSA-N 3-chloro-4-(hydroxymethyl)phenol Chemical compound OCC1=CC=C(O)C=C1Cl BDQASBWZSSWBCP-UHFFFAOYSA-N 0.000 description 1
- TXFPEBPIARQUIG-UHFFFAOYSA-N 4'-hydroxyacetophenone Chemical compound CC(=O)C1=CC=C(O)C=C1 TXFPEBPIARQUIG-UHFFFAOYSA-N 0.000 description 1
- HSKGHUJMBLYPDO-UHFFFAOYSA-N 4-(hydroxymethyl)-2-methylphenol Chemical compound CC1=CC(CO)=CC=C1O HSKGHUJMBLYPDO-UHFFFAOYSA-N 0.000 description 1
- LKVFCSWBKOVHAH-UHFFFAOYSA-N 4-Ethoxyphenol Chemical compound CCOC1=CC=C(O)C=C1 LKVFCSWBKOVHAH-UHFFFAOYSA-N 0.000 description 1
- UPMQABMXFIXTKX-UHFFFAOYSA-N 4-chloro-2-(3-hydroxypropyl)phenol Chemical compound OCCCC1=CC(Cl)=CC=C1O UPMQABMXFIXTKX-UHFFFAOYSA-N 0.000 description 1
- ZPNJDGLWSCMYIJ-UHFFFAOYSA-N 4-chloro-3-(hydroxymethyl)phenol Chemical compound OCC1=CC(O)=CC=C1Cl ZPNJDGLWSCMYIJ-UHFFFAOYSA-N 0.000 description 1
- DVKVZPIRWWREJC-UHFFFAOYSA-N 4-chloro-3-ethylphenol Chemical compound CCC1=CC(O)=CC=C1Cl DVKVZPIRWWREJC-UHFFFAOYSA-N 0.000 description 1
- WXNZTHHGJRFXKQ-UHFFFAOYSA-N 4-chlorophenol Chemical compound OC1=CC=C(Cl)C=C1 WXNZTHHGJRFXKQ-UHFFFAOYSA-N 0.000 description 1
- QEYQMWSESURNPP-UHFFFAOYSA-N 4-propan-2-yloxyphenol Chemical compound CC(C)OC1=CC=C(O)C=C1 QEYQMWSESURNPP-UHFFFAOYSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 108010008604 L-alpha-glycerol-phosphate oxidase Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 101710132772 Peroxidase 1 Proteins 0.000 description 1
- 241000577218 Phenes Species 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 108010060059 Sarcosine Oxidase Proteins 0.000 description 1
- 102000008118 Sarcosine oxidase Human genes 0.000 description 1
- 206010041235 Snoring Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 229960003624 creatine Drugs 0.000 description 1
- 239000006046 creatine Substances 0.000 description 1
- 229940109239 creatinine Drugs 0.000 description 1
- 125000004989 dicarbonyl group Chemical group 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- LSAJWVANLGVYBA-UHFFFAOYSA-N methyl 5-hydroxy-2-(hydroxymethyl)benzoate Chemical compound COC(=O)C1=CC(O)=CC=C1CO LSAJWVANLGVYBA-UHFFFAOYSA-N 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- CIPVVROJHKLHJI-UHFFFAOYSA-N n,n-diethyl-3-methylaniline Chemical compound CCN(CC)C1=CC=CC(C)=C1 CIPVVROJHKLHJI-UHFFFAOYSA-N 0.000 description 1
- ZPEDSBOBSNNATM-UHFFFAOYSA-N n-[2-(n-ethyl-3-methylanilino)ethyl]acetamide Chemical compound CC(=O)NCCN(CC)C1=CC=CC(C)=C1 ZPEDSBOBSNNATM-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229940076788 pyruvate Drugs 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 125000001174 sulfone group Chemical group 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5928582A JPS58175496A (ja) | 1982-04-08 | 1982-04-08 | 基質または酵素活性の定量方法 |
| US06/823,836 US4778757A (en) | 1982-04-08 | 1986-01-30 | Method for the determination of substrates or enzyme activities |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5928582A JPS58175496A (ja) | 1982-04-08 | 1982-04-08 | 基質または酵素活性の定量方法 |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11386983A Division JPS5911196A (ja) | 1982-04-08 | 1983-06-23 | 基質または酵素活性の定量方法 |
| JP11387083A Division JPS5911197A (ja) | 1982-04-08 | 1983-06-23 | 基質または酵素活性の定量方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58175496A true JPS58175496A (ja) | 1983-10-14 |
| JPS6237960B2 JPS6237960B2 (enrdf_load_stackoverflow) | 1987-08-14 |
Family
ID=13108964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5928582A Granted JPS58175496A (ja) | 1982-04-08 | 1982-04-08 | 基質または酵素活性の定量方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58175496A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015519038A (ja) * | 2012-03-23 | 2015-07-09 | サーモディクス，インコーポレイティド | スルホン酸を含むインビトロの診断テスト用組成物および方法 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01171024U (enrdf_load_stackoverflow) * | 1988-05-20 | 1989-12-04 | ||
| JPH0324U (enrdf_load_stackoverflow) * | 1989-05-19 | 1991-01-07 |
-
1982
- 1982-04-08 JP JP5928582A patent/JPS58175496A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015519038A (ja) * | 2012-03-23 | 2015-07-09 | サーモディクス，インコーポレイティド | スルホン酸を含むインビトロの診断テスト用組成物および方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6237960B2 (enrdf_load_stackoverflow) | 1987-08-14 |
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