JPS58167944A - 螢光偏極測定装置 - Google Patents
螢光偏極測定装置Info
- Publication number
- JPS58167944A JPS58167944A JP58038892A JP3889283A JPS58167944A JP S58167944 A JPS58167944 A JP S58167944A JP 58038892 A JP58038892 A JP 58038892A JP 3889283 A JP3889283 A JP 3889283A JP S58167944 A JPS58167944 A JP S58167944A
- Authority
- JP
- Japan
- Prior art keywords
- polarization
- light
- fluorescence
- excitation
- beams
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005259 measurement Methods 0.000 claims description 30
- 230000010287 polarization Effects 0.000 claims description 25
- 230000005284 excitation Effects 0.000 claims description 18
- 238000002875 fluorescence polarization Methods 0.000 claims description 13
- 238000012937 correction Methods 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 description 6
- 238000004364 calculation method Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 3
- 206010034972 Photosensitivity reaction Diseases 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000036211 photosensitivity Effects 0.000 description 2
- 238000012935 Averaging Methods 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-N Propionic acid Chemical compound CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000005315 distribution function Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011163 secondary particle Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
- G01N2030/8429—Preparation of the fraction to be distributed adding modificating material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/92—Construction of the plate
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823208919 DE3208919A1 (de) | 1982-03-12 | 1982-03-12 | Anordnung zur messung der fluoreszenzpolarisation |
| DE3208919.8 | 1982-03-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS58167944A true JPS58167944A (ja) | 1983-10-04 |
Family
ID=6157996
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58038892A Pending JPS58167944A (ja) | 1982-03-12 | 1983-03-09 | 螢光偏極測定装置 |
Country Status (5)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007533971A (ja) * | 2003-09-30 | 2007-11-22 | シンギュレックス・インコーポレイテッド | 粒子検出の分析精度を改善する方法 |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1376042A1 (ru) * | 1985-05-12 | 1988-02-23 | Институт физики АН БССР | Лазерный флуориметрический детектор дл микроколоночной хроматографии |
| US4842404A (en) * | 1988-01-13 | 1989-06-27 | Ilc Technology, Inc. | Dual detector laser beam power monitor |
| US5206699A (en) * | 1988-05-06 | 1993-04-27 | Gersan Establishment | Sensing a narrow frequency band of radiation and gemstones |
| US5760900A (en) * | 1989-03-18 | 1998-06-02 | Canon Kabushiki Kaisha | Method and apparatus for optically measuring specimen |
| JPH03140840A (ja) * | 1989-10-26 | 1991-06-14 | Hitachi Ltd | 流動細胞分析装置 |
| DE69028687T2 (de) * | 1989-12-15 | 1997-04-10 | Canon Kk | Vorrichtung zur optischen Messung einer Probe |
| US5445935A (en) | 1992-11-23 | 1995-08-29 | Royer; Catherine A. | Quantitative detection of macromolecules with fluorescent oligonucleotides |
| DE19612569C1 (de) * | 1996-03-29 | 1997-10-23 | Palas Gmbh | Verfahren und Vorrichtung zum Prüfen der Größenverteilung von Partikeln in Aerosolen |
| WO2001063260A1 (en) * | 2000-02-25 | 2001-08-30 | Cambridge Research & Instrumentation Inc. | Instantaneous dual band fluorescence detection systems |
| DK1290427T3 (en) * | 2000-04-11 | 2017-12-04 | Chemometec As | Method and apparatus for detecting the fluorescence of a sample |
| US20080021674A1 (en) * | 2003-09-30 | 2008-01-24 | Robert Puskas | Methods for Enhancing the Analysis of Particle Detection |
| US6970241B1 (en) | 2004-08-24 | 2005-11-29 | Desa Richard J | Device for enabling slow and direct measurement of fluorescence polarization |
| US20080225265A1 (en) * | 2007-03-17 | 2008-09-18 | Mi Research, Inc. | Method and apparatus for finding macromolecule crystallization conditions |
| US8269191B2 (en) * | 2010-11-05 | 2012-09-18 | The United States Of America As Represented By The Secretary Of The Army | System and method for measuring depolarization |
| DE102015007506B3 (de) * | 2015-06-11 | 2016-12-15 | Sensor Instruments Entwicklungs- Und Vertriebs Gmbh | Vorrichtung und Verfahren zur Bestimmung einer Partikelgröße in einem Sprühstrahl |
| CN110927025B (zh) * | 2019-12-05 | 2023-03-10 | 北京华泰诺安探测技术有限公司 | 一种气溶胶粒子监测设备 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL49622A0 (en) * | 1976-05-21 | 1976-07-30 | Elscint Ltd | A method and apparatus for classifying biological cells |
| US4203670A (en) * | 1977-04-21 | 1980-05-20 | Bromberg Nathan S | System and method of fluorescence polarimetry |
| DE2732272C2 (de) * | 1977-07-16 | 1979-07-05 | Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts, 6900 Heidelberg | Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen |
| DE2943116C2 (de) * | 1979-10-25 | 1986-06-19 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Einrichtung zur durchflußcytometrischen Reaktions- und/oder Diffusionsmessung |
| US4419583A (en) * | 1980-05-31 | 1983-12-06 | Noeller Hans Guenter | Polarization fluoroimmunoassay with pulsed light source |
-
1982
- 1982-03-12 DE DE19823208919 patent/DE3208919A1/de active Granted
- 1982-12-21 FR FR8221469A patent/FR2523305B1/fr not_active Expired
-
1983
- 1983-02-23 GB GB08304977A patent/GB2119924B/en not_active Expired
- 1983-02-28 US US06/470,282 patent/US4548499A/en not_active Expired - Fee Related
- 1983-03-09 JP JP58038892A patent/JPS58167944A/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007533971A (ja) * | 2003-09-30 | 2007-11-22 | シンギュレックス・インコーポレイテッド | 粒子検出の分析精度を改善する方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3208919C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1992-03-26 |
| FR2523305B1 (fr) | 1987-02-27 |
| GB8304977D0 (en) | 1983-03-30 |
| DE3208919A1 (de) | 1983-09-22 |
| GB2119924B (en) | 1985-08-29 |
| GB2119924A (en) | 1983-11-23 |
| FR2523305A1 (fr) | 1983-09-16 |
| US4548499A (en) | 1985-10-22 |
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