JPS578447A - Method and apparatus for analysis of guanidine derivative - Google Patents

Method and apparatus for analysis of guanidine derivative

Info

Publication number
JPS578447A
JPS578447A JP8413680A JP8413680A JPS578447A JP S578447 A JPS578447 A JP S578447A JP 8413680 A JP8413680 A JP 8413680A JP 8413680 A JP8413680 A JP 8413680A JP S578447 A JPS578447 A JP S578447A
Authority
JP
Japan
Prior art keywords
fluorescence
reaction
solution
reagent
sent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP8413680A
Other languages
Japanese (ja)
Other versions
JPH0210380B2 (en
Inventor
Toshio Kinoshita
Yayoi Hiraga
Yasuo Ishida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP8413680A priority Critical patent/JPS578447A/en
Publication of JPS578447A publication Critical patent/JPS578447A/en
Publication of JPH0210380B2 publication Critical patent/JPH0210380B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed

Abstract

PURPOSE:To make a determination of guanidine derivates in a short time with a good sensibility, by adding an aqueous solution of ninhydrin after adding the aqueous sodium hydroxide solution at first as a fluorescent reagent. CONSTITUTION:A sample containing a very small amount of guanidine derivatives is injected into a column 4 of high speed liquid chromatography connected with a stepwise gradient apparatus 1 and a feeding pump 2 for high speed liquid chromatography from a sample introducing opening 3. An aqueous sodium hydroxide solution 5 and an aqueous solution of nihydrin 7 are consecutively added to said eluate by using feeding pumps of reagent 6, 8 and the reaction is performed. Said mixed solution is sent to a reaction coil 10 of fluorescence reaction part 9 and the fluorescence reaction is performed. Next, the reacted solution is sent to a fluorescence photometer 11 and an intensity of fluorescence is measured and then, guanidine derivatives are determined. Hereby, conventional amino acid does not emit fluorescence and also, the analysis is not interrupted by any deposited matter because the reagent is soluble in water.
JP8413680A 1980-06-20 1980-06-20 Method and apparatus for analysis of guanidine derivative Granted JPS578447A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8413680A JPS578447A (en) 1980-06-20 1980-06-20 Method and apparatus for analysis of guanidine derivative

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8413680A JPS578447A (en) 1980-06-20 1980-06-20 Method and apparatus for analysis of guanidine derivative

Publications (2)

Publication Number Publication Date
JPS578447A true JPS578447A (en) 1982-01-16
JPH0210380B2 JPH0210380B2 (en) 1990-03-07

Family

ID=13822073

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8413680A Granted JPS578447A (en) 1980-06-20 1980-06-20 Method and apparatus for analysis of guanidine derivative

Country Status (1)

Country Link
JP (1) JPS578447A (en)

Also Published As

Publication number Publication date
JPH0210380B2 (en) 1990-03-07

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