GB1349491A - Fluorimetric assay of primary amino groups - Google Patents

Fluorimetric assay of primary amino groups

Info

Publication number
GB1349491A
GB1349491A GB4872171A GB4872171A GB1349491A GB 1349491 A GB1349491 A GB 1349491A GB 4872171 A GB4872171 A GB 4872171A GB 4872171 A GB4872171 A GB 4872171A GB 1349491 A GB1349491 A GB 1349491A
Authority
GB
United Kingdom
Prior art keywords
reagent
primary amino
oct
determination
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4872171A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of GB1349491A publication Critical patent/GB1349491A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1349491 Determination of primary amino group containing compounds F HOFFMANNLA ROCHE & CO AG 20 Oct 1971 [21 Oct 1970] 48721/71 Heading B1X A reagent for the determination of primary amine group-containing compounds comprises a solution in an inert organic solvent of ninhydrin and an aryl alkyl aldehyde of formula Ar(CH 2 )n CHO, where n is 1 to 8. The reagent is mixed with the sample, the mixture is heated to produce a fluorescent substance and the amount of fluorescent substance is measured. The test may be carried out at pH 4 to 9, preferably 6 to 8. The sample may be the effluent from a chromatography column, when the effluent stream is mixed with a stream of reagent and buffer solution and passed through a mixing coil and a heating bath into a continuous flow cell of a fluorophotometer. The reagent may also be used for developing paper or thin layer chromatograms.
GB4872171A 1970-10-21 1971-10-20 Fluorimetric assay of primary amino groups Expired GB1349491A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8259670A 1970-10-21 1970-10-21

Publications (1)

Publication Number Publication Date
GB1349491A true GB1349491A (en) 1974-04-03

Family

ID=22172164

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4872171A Expired GB1349491A (en) 1970-10-21 1971-10-20 Fluorimetric assay of primary amino groups

Country Status (9)

Country Link
US (1) US3689221A (en)
AU (1) AU3442271A (en)
DD (1) DD94907A5 (en)
DE (1) DE2148603A1 (en)
FR (1) FR2110033A5 (en)
GB (1) GB1349491A (en)
IL (1) IL37847A0 (en)
NL (1) NL7114508A (en)
ZA (1) ZA716604B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2735877A1 (en) * 2012-11-21 2014-05-28 JPP Chromatography Limited A novel ninhydrin reagent for analysing nitrogen-containing compounds
US9250191B2 (en) 2012-11-21 2016-02-02 Jpp Chromatography Limited Method for analysing amino acids and a reagent for use with the same

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3812181A (en) * 1971-12-27 1974-05-21 Hoffmann La Roche O-(alpha-hydroxycinnamoyl)benzoic acid and related compounds
US3830629A (en) * 1972-10-18 1974-08-20 Hoffmann La Roche Fluorometric analysis of secondary alpha-amino acids
US3892530A (en) * 1974-09-23 1975-07-01 Hoffmann La Roche Colorimetric and fluorometric method
US3994688A (en) * 1975-04-30 1976-11-30 A/S N. Foss Electric Method and reagent for quantitative analysis of l-lysine in proteinaceous test substances
US4274833A (en) * 1979-10-18 1981-06-23 Pickering Michael V Ninhydrin reagent for use in amine and amino acid analyses
US4253848A (en) * 1980-01-21 1981-03-03 The United States Of America As Represented By The Secretary Of The Army Method for determining oxidative status of unsaturated lipids and method for evaluating antioxidant effectiveness in lipid-polypeptide layers
US6696304B1 (en) 1999-02-24 2004-02-24 Luminex Corporation Particulate solid phase immobilized protein quantitation
CN110687235B (en) * 2019-10-18 2023-01-31 贵州省烟草科学研究院 Method for analyzing nicotine isomer in fresh tobacco leaves

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2735877A1 (en) * 2012-11-21 2014-05-28 JPP Chromatography Limited A novel ninhydrin reagent for analysing nitrogen-containing compounds
GB2508147A (en) * 2012-11-21 2014-05-28 Jpp Chromatography Ltd A ninhydrin reagent including ninhydrin and a reducing agent
WO2014080158A1 (en) * 2012-11-21 2014-05-30 Jpp Chromatography Limited A novel ninhydrin reagent for analysing nitrogen-containing compounds
CN104919320A (en) * 2012-11-21 2015-09-16 Jpp色谱有限公司 A novel ninhydrin reagent for analysing nitrogen-containing compounds
JP2015536464A (en) * 2012-11-21 2015-12-21 ジェイピーピー クロマトグラフィー リミテッド A novel ninhydrin reagent for the analysis of nitrogen-containing compounds
US9250191B2 (en) 2012-11-21 2016-02-02 Jpp Chromatography Limited Method for analysing amino acids and a reagent for use with the same
US9250190B2 (en) 2012-11-21 2016-02-02 Jpp Chromatography Limited Reagent and method using the same
GB2508147B (en) * 2012-11-21 2016-06-01 Jpp Chromatography Ltd Ninhydrin reagent for use in a method of analysing nitrogen-containing compounds
CN104919320B (en) * 2012-11-21 2017-05-31 Jpp色谱有限公司 New ninhydrin reagent for analyzing nitrogen-containing compound

Also Published As

Publication number Publication date
IL37847A0 (en) 1971-12-29
FR2110033A5 (en) 1972-05-26
NL7114508A (en) 1972-04-25
DE2148603A1 (en) 1972-05-04
AU3442271A (en) 1973-04-19
US3689221A (en) 1972-09-05
ZA716604B (en) 1972-06-28
DD94907A5 (en) 1973-01-05

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PLNP Patent lapsed through nonpayment of renewal fees