SU819712A1 - Method of amino acid detection in chromatographic analysis - Google Patents
Method of amino acid detection in chromatographic analysis Download PDFInfo
- Publication number
- SU819712A1 SU819712A1 SU762335778A SU2335778A SU819712A1 SU 819712 A1 SU819712 A1 SU 819712A1 SU 762335778 A SU762335778 A SU 762335778A SU 2335778 A SU2335778 A SU 2335778A SU 819712 A1 SU819712 A1 SU 819712A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- amino acid
- chromatographic analysis
- acid detection
- ninhydrin
- reaction
- Prior art date
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Description
(54) СПОСОВ ДЕТЕКТИРОВАНИЯ АМИНОКИСЛОТ ПРИ ХРОМАТОГРАФИЧЕСКОМ АНАЛИЗЕ(54) WAYS OF DETECTING AMINO ACIDS IN CHROMATOGRAPHIC ANALYSIS
предложенного состава термически более стабилен, чем нингидриновый реагент известного состава. Использование предлагаемого реагента позвол ет подн ть температуру детектировани до 160-165с, причем в этих услови х реакци завершаетс з более короткое врем (см. таблицу), что обеспечивает малый вклад размывани в реакторе в общее размывание хроматографической зоны и позвол ет повысить чувствительность.The proposed composition is thermally more stable than the ninhydrin reagent of known composition. The use of the proposed reagent allows the detection temperature to be raised to 160-165 s, and under these conditions the reaction is completed in a shorter time (see table), which provides a small contribution to erosion in the reactor to the overall erosion of the chromatographic zone and allows for increased sensitivity.
Из таблицы видно, что температура реакции 160-165 0 вл етс оптимальной , так как при дальнейшем пот вышении температуры возрастает термодеструкци окрашенного продукта. С другой стороны в области рН 4,85 ,2 температура термодеструкции максимальна, а нингидриновый реаген отличаетс наибольшей стабильностьюFrom the table it can be seen that the reaction temperature of 160-165 0 is optimal, since with further sweating of the temperature, the thermal decomposition of the colored product increases. On the other hand, in the pH range 4.85, 2, the temperature of thermal decomposition is maximum, and the ninhydrin reagent is characterized by the highest stability
Исследовалась реакци с нингидрином аминокислоты валин (концен- траци ) и иминокислоты проли ( концентраци 10 М).The reaction with the ninhydrin amino acid valine (concentration) and imino acid of prol (concentration 10 M) was investigated.
Реакци осуществл лась в проточном реакторе из фторопластового капилл ра с внутренним диаметром 0,8 мм, на выходе которого находилось гидродинамическое сопротивление - капилл р 0,3 мм, длиной 3,5 м, - что достаточно дл создани в реакторе давлени 3 атм. Врем реакции задавалось скоростью подачи реагентов в смеситель (1 мл/мин дл раствора аминокислоты и дл нингидри нового реактива) и изменением длины реактора (10 сек при 33,5 см 1,5 мин при 302 см и 15 мин при 3-10 см). Прореагировавша смесь на выходе из реактора охлаждалась и направл лась в детектор-спектрофотометр с длиной кюветы 1 см, где измер лась разница оптических плотностей продукта реакции и разбавленного вдвое нингидринового реагента наThe reaction was carried out in a flow reactor made of a fluoroplastic capillary with an internal diameter of 0.8 mm, at the outlet of which there was a hydrodynamic resistance — a capillary 0.3 mm and a length of 3.5 m — which is enough to create a pressure of 3 atm in the reactor. The reaction time was set by the feed rate of the reactants to the mixer (1 ml / min for the amino acid solution and for the ninhydric reagent) and the change in the length of the reactor (10 sec at 33.5 cm for 1.5 min at 302 cm and 15 min at 3-10 cm) . The reacted mixture at the outlet of the reactor was cooled and sent to a detector-spectrophotometer with a cuvette length of 1 cm, where the difference in optical densities of the reaction product and the ninhydrin reagent diluted by half was measured.
0 длине волны 570 нм дл валина и 440 нм дл пролина.0 wavelength of 570 nm for valine and 440 nm for proline.
Изобретение позвол ет ускорить . врем завершени нингидриновой реакции и повысить чувствительность хроматографии аминокислот.The invention speeds up. the completion time of the ninhydrin reaction and increase the sensitivity of amino acid chromatography.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762335778A SU819712A1 (en) | 1976-03-19 | 1976-03-19 | Method of amino acid detection in chromatographic analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762335778A SU819712A1 (en) | 1976-03-19 | 1976-03-19 | Method of amino acid detection in chromatographic analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU819712A1 true SU819712A1 (en) | 1981-04-07 |
Family
ID=20652737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762335778A SU819712A1 (en) | 1976-03-19 | 1976-03-19 | Method of amino acid detection in chromatographic analysis |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU819712A1 (en) |
-
1976
- 1976-03-19 SU SU762335778A patent/SU819712A1/en active
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