JPS5722698A - Determination of got activity - Google Patents

Determination of got activity

Info

Publication number
JPS5722698A
JPS5722698A JP9756380A JP9756380A JPS5722698A JP S5722698 A JPS5722698 A JP S5722698A JP 9756380 A JP9756380 A JP 9756380A JP 9756380 A JP9756380 A JP 9756380A JP S5722698 A JPS5722698 A JP S5722698A
Authority
JP
Japan
Prior art keywords
specimen
activity
reaction liquid
cysteine sulfinate
oxoglutarate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9756380A
Other languages
Japanese (ja)
Other versions
JPS6366520B2 (en
Inventor
Mitsuhiro Nozaki
Hiroyuki Kagamiyama
Toshiharu Yagi
Kenji Soda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KOKUSAI SHAKU KK
KOKUSAI SHIYAKU KK
Original Assignee
KOKUSAI SHAKU KK
KOKUSAI SHIYAKU KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KOKUSAI SHAKU KK, KOKUSAI SHIYAKU KK filed Critical KOKUSAI SHAKU KK
Priority to JP9756380A priority Critical patent/JPS5722698A/en
Publication of JPS5722698A publication Critical patent/JPS5722698A/en
Publication of JPS6366520B2 publication Critical patent/JPS6366520B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the GOT activity of a specimen in high specificity, by adding the specimen to a reaction liquid containing a substrate consisting of cysteine sulfinate and 2-oxoglutarate or cysteine sulfinate and oxaloacetate, an intermediate electron carrier, and a tetrazolium salt compound.
CONSTITUTION: The specimen is added to a reaction liquid containing (A) a substrate consisting of cysteine sulfinate and 2-oxoglutarate or cysteine sulfinate and oxaloacetate, (B) an intermediate electron carrier such as phenazine, metho.sulfate, etc., and (C) a tetrazolium salt compound such as 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide, and the absorbance of the mixture is measured. As an alternative method, the specimen is subjected to electrophoretic treatment and immersed in the above reaction liquid, and its color density is measured by densitmetry to determine the GOT activity.
COPYRIGHT: (C)1982,JPO&Japio
JP9756380A 1980-07-18 1980-07-18 Determination of got activity Granted JPS5722698A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9756380A JPS5722698A (en) 1980-07-18 1980-07-18 Determination of got activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9756380A JPS5722698A (en) 1980-07-18 1980-07-18 Determination of got activity

Publications (2)

Publication Number Publication Date
JPS5722698A true JPS5722698A (en) 1982-02-05
JPS6366520B2 JPS6366520B2 (en) 1988-12-21

Family

ID=14195693

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9756380A Granted JPS5722698A (en) 1980-07-18 1980-07-18 Determination of got activity

Country Status (1)

Country Link
JP (1) JPS5722698A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981787A (en) * 1989-02-14 1991-01-01 Xytronyx, Inc. Method for diagnosis of periodontal disease by detection of L-alanine aminotransferase
US5047328A (en) * 1988-10-26 1991-09-10 The Board Of Trustees Of The University Of Illinois Method for determination of the type and severity of periodontal disease states

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5047328A (en) * 1988-10-26 1991-09-10 The Board Of Trustees Of The University Of Illinois Method for determination of the type and severity of periodontal disease states
US4981787A (en) * 1989-02-14 1991-01-01 Xytronyx, Inc. Method for diagnosis of periodontal disease by detection of L-alanine aminotransferase

Also Published As

Publication number Publication date
JPS6366520B2 (en) 1988-12-21

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