JPS57132884A - Immobilizing method of enzyme - Google Patents

Immobilizing method of enzyme

Info

Publication number
JPS57132884A
JPS57132884A JP1851781A JP1851781A JPS57132884A JP S57132884 A JPS57132884 A JP S57132884A JP 1851781 A JP1851781 A JP 1851781A JP 1851781 A JP1851781 A JP 1851781A JP S57132884 A JPS57132884 A JP S57132884A
Authority
JP
Japan
Prior art keywords
enzyme
added
hydrophobic
monomers
buffer solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1851781A
Other languages
Japanese (ja)
Other versions
JPS6219836B2 (en
Inventor
Masaru Yoshida
Isao Kaetsu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NIPPON GENSHIRYOKU KENKYUSHO
Japan Atomic Energy Agency
Original Assignee
NIPPON GENSHIRYOKU KENKYUSHO
Japan Atomic Energy Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NIPPON GENSHIRYOKU KENKYUSHO, Japan Atomic Energy Research Institute filed Critical NIPPON GENSHIRYOKU KENKYUSHO
Priority to JP1851781A priority Critical patent/JPS57132884A/en
Publication of JPS57132884A publication Critical patent/JPS57132884A/en
Publication of JPS6219836B2 publication Critical patent/JPS6219836B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PURPOSE: To immobilize an enzyme at a high immobilizing ratio, by giving a porous structure to a hydrophobic polymeric matrix, and entrapping an enzyme therein.
CONSTITUTION: One or two or more hydrophobic polymerizable monomers polymerizable at 0°C or below, e.g. glycidyl methacrylate or ethylene glycol dimethacrylate, are added to a buffer solution at a suitable pH containing one or two or more enzymes, and if necessary a solvent capable of dissolving both of the buffer solution and the monomers, e.g. polyethylene glycol or an alcohol, is added thereto, or a hydrophilic polymerizable monomer, e.g. hydroxyethyl methacrylate or hydroxyethyl acrylate, is added thereto. The resultant mixture is then mixed thoroughly under mechanical stirring to form a water-in-oil emulsion, which is then irradiated with light or ionizing radiation to polymerize the hydrophobic polymerizable monomers.
COPYRIGHT: (C)1982,JPO&Japio
JP1851781A 1981-02-10 1981-02-10 Immobilizing method of enzyme Granted JPS57132884A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1851781A JPS57132884A (en) 1981-02-10 1981-02-10 Immobilizing method of enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1851781A JPS57132884A (en) 1981-02-10 1981-02-10 Immobilizing method of enzyme

Publications (2)

Publication Number Publication Date
JPS57132884A true JPS57132884A (en) 1982-08-17
JPS6219836B2 JPS6219836B2 (en) 1987-05-01

Family

ID=11973814

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1851781A Granted JPS57132884A (en) 1981-02-10 1981-02-10 Immobilizing method of enzyme

Country Status (1)

Country Link
JP (1) JPS57132884A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5934886A (en) * 1982-08-24 1984-02-25 Japan Atom Energy Res Inst Immobilization of enzyme to surface of porous particle
JPS59154988A (en) * 1983-02-23 1984-09-04 Nitto Electric Ind Co Ltd Water-dispersible polymer particles and immobilized enzyme using said particles as carrier

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5934886A (en) * 1982-08-24 1984-02-25 Japan Atom Energy Res Inst Immobilization of enzyme to surface of porous particle
JPH0361426B2 (en) * 1982-08-24 1991-09-19 Japan Atomic Energy Res Inst
JPS59154988A (en) * 1983-02-23 1984-09-04 Nitto Electric Ind Co Ltd Water-dispersible polymer particles and immobilized enzyme using said particles as carrier
JPH0229312B2 (en) * 1983-02-23 1990-06-28 Nitto Denko Corp

Also Published As

Publication number Publication date
JPS6219836B2 (en) 1987-05-01

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