JPS57130999A - Purification of ribonucleic acid - Google Patents
Purification of ribonucleic acidInfo
- Publication number
- JPS57130999A JPS57130999A JP1579381A JP1579381A JPS57130999A JP S57130999 A JPS57130999 A JP S57130999A JP 1579381 A JP1579381 A JP 1579381A JP 1579381 A JP1579381 A JP 1579381A JP S57130999 A JPS57130999 A JP S57130999A
- Authority
- JP
- Japan
- Prior art keywords
- ribonucleic acid
- carbonate
- adjusted
- water
- alkali metal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To obtain ribonucleic acid free from alkaline earth metals, by mixing ribonucleic acid with alkali matal carbonate or ammonium carbonate and water, reacting the components under slightly alkaline conditions, and removing the produced precipitate.
CONSTITUTION: Ribonucleic acid is mixed with one or more compounds selected from alkali metal carbonates and ammonium carbonate, and water, the pH of the mixture is adjusted to slightly alkaline state, pref. 9W10pH, and the components are reacted with each other at 30W40°C. The alkaline earth metal in the ribonucleic acid is exchanged with alkali metal or ammonia, and is precipitated as the carbonate. If necessary, the pH of the solution is adjusted to neutral, and the precipitate is removed by filtration, centrifugal separation, etc.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1579381A JPS57130999A (en) | 1981-02-06 | 1981-02-06 | Purification of ribonucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1579381A JPS57130999A (en) | 1981-02-06 | 1981-02-06 | Purification of ribonucleic acid |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS57130999A true JPS57130999A (en) | 1982-08-13 |
Family
ID=11898708
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1579381A Pending JPS57130999A (en) | 1981-02-06 | 1981-02-06 | Purification of ribonucleic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57130999A (en) |
-
1981
- 1981-02-06 JP JP1579381A patent/JPS57130999A/en active Pending
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