JPS57101765A - Method for assaying specific serum protein - Google Patents

Method for assaying specific serum protein

Info

Publication number
JPS57101765A
JPS57101765A JP17716480A JP17716480A JPS57101765A JP S57101765 A JPS57101765 A JP S57101765A JP 17716480 A JP17716480 A JP 17716480A JP 17716480 A JP17716480 A JP 17716480A JP S57101765 A JPS57101765 A JP S57101765A
Authority
JP
Japan
Prior art keywords
protein
water
denaturing agent
serum protein
given amount
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17716480A
Other languages
Japanese (ja)
Inventor
Mitsuo Otsuka
Shinichi Gamachi
Kenji Chichibu
Kiyoshige Wakabayashi
Kenji Kumaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Priority to JP17716480A priority Critical patent/JPS57101765A/en
Publication of JPS57101765A publication Critical patent/JPS57101765A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To provide a simple B-protein assay method not using complicated natural extract such as GoA-SPC. CONSTITUTION:A chemical denaturing agent is added to a given amount of human serum diluted with water or an appropriate salt solution or non-diluted to produce a precipitate. The produced precipitate is separated by a conventional method and again suspended in a given amount of water or an appropriate salt solution to dissolve a water-soluble part, and turbidity is measured by a conventional means to assay serum protein, especially B-protein. A protein denaturing agent is used as the chemical denaturing agent, and is preferably trichloroacetic acid, methanol, ethanol, etc., among which trichloroacetic acid, used as a 10-20% aqueous solution, is most suitable.
JP17716480A 1980-12-17 1980-12-17 Method for assaying specific serum protein Pending JPS57101765A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17716480A JPS57101765A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17716480A JPS57101765A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Publications (1)

Publication Number Publication Date
JPS57101765A true JPS57101765A (en) 1982-06-24

Family

ID=16026294

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17716480A Pending JPS57101765A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Country Status (1)

Country Link
JP (1) JPS57101765A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06502244A (en) * 1990-11-14 1994-03-10 アクシス　リサーチ　エイエス Assay for glycanated blood proteins
JP5653447B2 (en) * 2010-10-12 2015-01-14 株式会社日立製作所 Biological simulation phantom and calibration device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06502244A (en) * 1990-11-14 1994-03-10 アクシス　リサーチ　エイエス Assay for glycanated blood proteins
JP5653447B2 (en) * 2010-10-12 2015-01-14 株式会社日立製作所 Biological simulation phantom and calibration device

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