JPS57101764A - Method for assaying specific serum protein - Google Patents

Method for assaying specific serum protein

Info

Publication number
JPS57101764A
JPS57101764A JP17716380A JP17716380A JPS57101764A JP S57101764 A JPS57101764 A JP S57101764A JP 17716380 A JP17716380 A JP 17716380A JP 17716380 A JP17716380 A JP 17716380A JP S57101764 A JPS57101764 A JP S57101764A
Authority
JP
Japan
Prior art keywords
protein
serum
soluble
water
conventional means
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17716380A
Other languages
Japanese (ja)
Inventor
Mitsuo Otsuka
Kenji Chichibu
Kiyoshige Wakabayashi
Kenji Kumaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Priority to JP17716380A priority Critical patent/JPS57101764A/en
Publication of JPS57101764A publication Critical patent/JPS57101764A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To provide a simple B-protein assay method not using a complicated natural extract such as GoA-SPC. CONSTITUTION:A given amount of a soluble protein is added to a specified amount of human serum and mixed. A precipitate produced through the addition of a chemical denaturing agent is separated and collected by a conventional means and again suspended in a given amount of water or a salt solution to dissolve a water-soluble part, and the turbidity of the solution is measured by a conventional means to assay specific serum protein, especially B-protein. A substantially water-soluble protein having a molecular weight of about 13,000- 200,000 may be used as a soluble protein and serum albumin or serum globulin is especially preferred. A protein denaturing agnet is used as the chemical denaturing agent, and trichloroacetic acid used as a 10-20% aqueous solution is most suitable.
JP17716380A 1980-12-17 1980-12-17 Method for assaying specific serum protein Pending JPS57101764A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17716380A JPS57101764A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17716380A JPS57101764A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Publications (1)

Publication Number Publication Date
JPS57101764A true JPS57101764A (en) 1982-06-24

Family

ID=16026277

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17716380A Pending JPS57101764A (en) 1980-12-17 1980-12-17 Method for assaying specific serum protein

Country Status (1)

Country Link
JP (1) JPS57101764A (en)

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