JPS5696696A - Production of ribonucleic acid - Google Patents
Production of ribonucleic acidInfo
- Publication number
- JPS5696696A JPS5696696A JP17192679A JP17192679A JPS5696696A JP S5696696 A JPS5696696 A JP S5696696A JP 17192679 A JP17192679 A JP 17192679A JP 17192679 A JP17192679 A JP 17192679A JP S5696696 A JPS5696696 A JP S5696696A
- Authority
- JP
- Japan
- Prior art keywords
- ribonucleic acid
- bacillus
- mutant
- cultured
- surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE: A mutant that belongs to Bacillus and is sensitive to a surfactant and capable of producing ribonucleic acid is cultured to produce ribonucleic acid inexpensively.
CONSTITUTION: A mutant that belongs to Bacillus and is sensitive to a surfactant and capable of producing ribonucleic acid such as Bacillus subtilis N-32 (FERM- P5335) is inoculated to a liquid culture medium containing a carbon source, a nitrogen source, inorganic salts, when necessary, trace amounts of organic nourishment and cultured at a pH of 5.0W9.0 and 25W40°c for 2W7 days. The cells are removed from the culture mixture and the ribonucleic acid is extracted with phenol, then sodium acetate and cold ethanol are added to the extract to precipitate the ribonucleic acid.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17192679A JPS5818076B2 (en) | 1979-12-28 | 1979-12-28 | Method for producing ribonucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17192679A JPS5818076B2 (en) | 1979-12-28 | 1979-12-28 | Method for producing ribonucleic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5696696A true JPS5696696A (en) | 1981-08-04 |
JPS5818076B2 JPS5818076B2 (en) | 1983-04-11 |
Family
ID=15932400
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP17192679A Expired JPS5818076B2 (en) | 1979-12-28 | 1979-12-28 | Method for producing ribonucleic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5818076B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0215533A2 (en) * | 1985-01-18 | 1987-03-25 | Applied Biosystems, Inc. | A method of extracting nucleic acids from cells |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62111972U (en) * | 1986-01-07 | 1987-07-16 |
-
1979
- 1979-12-28 JP JP17192679A patent/JPS5818076B2/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0215533A2 (en) * | 1985-01-18 | 1987-03-25 | Applied Biosystems, Inc. | A method of extracting nucleic acids from cells |
Also Published As
Publication number | Publication date |
---|---|
JPS5818076B2 (en) | 1983-04-11 |
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