JPS5564800A - Method and reagent for determination of acyl coenzyme a - Google Patents

Method and reagent for determination of acyl coenzyme a

Info

Publication number
JPS5564800A
JPS5564800A JP13701178A JP13701178A JPS5564800A JP S5564800 A JPS5564800 A JP S5564800A JP 13701178 A JP13701178 A JP 13701178A JP 13701178 A JP13701178 A JP 13701178A JP S5564800 A JPS5564800 A JP S5564800A
Authority
JP
Japan
Prior art keywords
acyl coenzyme
reagent
oxidase
measurement
coenzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP13701178A
Other languages
Japanese (ja)
Other versions
JPS5729998B2 (en
Inventor
Koji Mizuno
Mitsuyoshi Toyosato
Hiromichi Hirakawa
Heihachiro Kinoshita
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Shoji Co Ltd
Original Assignee
Nippon Shoji Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Shoji Co Ltd filed Critical Nippon Shoji Co Ltd
Priority to JP13701178A priority Critical patent/JPS5564800A/en
Priority to DE19792944498 priority patent/DE2944498C2/en
Publication of JPS5564800A publication Critical patent/JPS5564800A/en
Publication of JPS5729998B2 publication Critical patent/JPS5729998B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP

Abstract

PURPOSE: To determine acyl coenzyme A useful for the clinical measurement of free fatty acids in blood, easily, accurately and rapidly, by measuring the H2O2 produced by the oxidative activity of acyl coenzyme A-oxidase.
CONSTITUTION: A liquid specimen containing acyl coenzyme A is adjusted to pH 7.0W8.5, and added with an acyl coenzyme A-oxidase, an SH reagent, a peroxidase or catalase, and a color developing reagent, separately or at a time. The mixture is heated at 37°C for 5W60min in an open atmosphere, and the optical density is measured using a light corresponding to the color of the liquid, e.g. a light having a wave length of 400W600nm. The amount of the acyl coenzyme A is determined from the calibration curve prepared by the process similar to the above. The addition of the SH reagent (a maleimide derivative, iodoacetamide, 4,4-bisdimethylamino-benzhydrol, etc.) improves the sensitivity of the measurement.
COPYRIGHT: (C)1980,JPO&Japio
JP13701178A 1978-11-06 1978-11-06 Method and reagent for determination of acyl coenzyme a Granted JPS5564800A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP13701178A JPS5564800A (en) 1978-11-06 1978-11-06 Method and reagent for determination of acyl coenzyme a
DE19792944498 DE2944498C2 (en) 1978-11-06 1979-11-03 Method for the quantitative determination of acyl-coenzyme A and reagent therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP13701178A JPS5564800A (en) 1978-11-06 1978-11-06 Method and reagent for determination of acyl coenzyme a

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP4346381A Division JPS578797A (en) 1981-03-24 1981-03-24 Method and reagent for quantitative determination of free fatty acid

Publications (2)

Publication Number Publication Date
JPS5564800A true JPS5564800A (en) 1980-05-15
JPS5729998B2 JPS5729998B2 (en) 1982-06-25

Family

ID=15188720

Family Applications (1)

Application Number Title Priority Date Filing Date
JP13701178A Granted JPS5564800A (en) 1978-11-06 1978-11-06 Method and reagent for determination of acyl coenzyme a

Country Status (2)

Country Link
JP (1) JPS5564800A (en)
DE (1) DE2944498C2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5743699A (en) * 1980-08-25 1982-03-11 Yatoron:Kk Measuring method of free fatty acid
US6686172B2 (en) 2000-06-30 2004-02-03 Azwell, Inc. Method of measuring total homocysteine

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5685300A (en) * 1979-12-12 1981-07-11 Amano Pharmaceut Co Ltd Determination of free fatty acid
JPS56158097A (en) * 1980-05-06 1981-12-05 Toyobo Co Ltd Quantitative determination of free fatty acid
JPS60217900A (en) * 1984-04-13 1985-10-31 Kyowa Medetsukusu Kk Method for determination of mercapto group-containing compound
JP4257730B2 (en) * 2003-08-25 2009-04-22 キッコーマン株式会社 Acyl CoA oxidase, its gene, recombinant DNA, and method for producing acyl CoA oxidase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5217085A (en) * 1975-07-30 1977-02-08 Ono Pharmaceut Co Ltd Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5743699A (en) * 1980-08-25 1982-03-11 Yatoron:Kk Measuring method of free fatty acid
JPS6366517B2 (en) * 1980-08-25 1988-12-21 Yatoron Kk
US6686172B2 (en) 2000-06-30 2004-02-03 Azwell, Inc. Method of measuring total homocysteine

Also Published As

Publication number Publication date
DE2944498A1 (en) 1980-05-14
DE2944498C2 (en) 1985-08-29
JPS5729998B2 (en) 1982-06-25

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