JPS5749862A - Method and reagent for determining unsaturated fatty acid hydroperoxide - Google Patents
Method and reagent for determining unsaturated fatty acid hydroperoxideInfo
- Publication number
- JPS5749862A JPS5749862A JP12535980A JP12535980A JPS5749862A JP S5749862 A JPS5749862 A JP S5749862A JP 12535980 A JP12535980 A JP 12535980A JP 12535980 A JP12535980 A JP 12535980A JP S5749862 A JPS5749862 A JP S5749862A
- Authority
- JP
- Japan
- Prior art keywords
- fatty acid
- unsaturated fatty
- specimen
- acid hydroperoxide
- peroxygenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- -1 unsaturated fatty acid hydroperoxide Chemical class 0.000 title abstract 4
- 235000021122 unsaturated fatty acids Nutrition 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 3
- 229910052760 oxygen Inorganic materials 0.000 abstract 3
- 239000001301 oxygen Substances 0.000 abstract 3
- 108010023506 peroxygenase Proteins 0.000 abstract 3
- BLRHMMGNCXNXJL-UHFFFAOYSA-N 1-methylindole Chemical compound C1=CC=C2N(C)C=CC2=C1 BLRHMMGNCXNXJL-UHFFFAOYSA-N 0.000 abstract 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 abstract 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007795 chemical reaction product Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 235000010582 Pisum sativum Nutrition 0.000 abstract 1
- 240000004713 Pisum sativum Species 0.000 abstract 1
- 125000002490 anilino group Chemical class [H]N(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 abstract 1
- 239000012490 blank solution Substances 0.000 abstract 1
- 238000011088 calibration curve Methods 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 abstract 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 210000001589 microsome Anatomy 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
PURPOSE:To determine N-methylindoxyl formed by incubating an unsaturated fatty acid hydroperoxide in a specimen with peroxygenase in the presence of N-methylindole as an oxygen acceptor. CONSTITUTION:To 0.1-1.0ml of a specimen, e.g., serum, maintained at 6.8- 8.0pH are added an oxygen acceptor and a peroxygenase and the mixture is heated at 370 deg.C for 10-40min. The reaction product is measured using a spectrophotometer with a blank solution as control. The same operation is conducted using the standard unsaturated fatty acid hydroperoxide, and the amount of an unsaturated fatty acid hydroperoxide in a specimen is obtained from a prepared calibration curve. The peroxygenase is prepared from a microsome fraction obtained from peas in its germination period. Indole, phenol and aniline derivatives are used as the oxygen acceptor, and a N-methylindole is the most suitable. The reaction product obtained from the above composition is stable, and so enables the measurement using a minute amount of the specimen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12535980A JPS5749862A (en) | 1980-09-11 | 1980-09-11 | Method and reagent for determining unsaturated fatty acid hydroperoxide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12535980A JPS5749862A (en) | 1980-09-11 | 1980-09-11 | Method and reagent for determining unsaturated fatty acid hydroperoxide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5749862A true JPS5749862A (en) | 1982-03-24 |
Family
ID=14908177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12535980A Pending JPS5749862A (en) | 1980-09-11 | 1980-09-11 | Method and reagent for determining unsaturated fatty acid hydroperoxide |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5749862A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002018942A1 (en) * | 2000-12-22 | 2002-03-07 | Orthomol Pharmazeutische Vertriebs Gmbh | Kit and method for determining the redox state in urine |
| WO2013189802A1 (en) * | 2012-06-19 | 2013-12-27 | Novozymes A/S | Enzymatic reduction of hydroperoxides |
| US11130969B2 (en) | 2013-07-12 | 2021-09-28 | Novozymes, A/S | Direct transfer of polynucleotides between genomes |
-
1980
- 1980-09-11 JP JP12535980A patent/JPS5749862A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002018942A1 (en) * | 2000-12-22 | 2002-03-07 | Orthomol Pharmazeutische Vertriebs Gmbh | Kit and method for determining the redox state in urine |
| US6835554B2 (en) | 2000-12-22 | 2004-12-28 | Orthomol Pharmazeutische Vertriebs Gmbh | Kit and method for determining redox status in urine |
| WO2013189802A1 (en) * | 2012-06-19 | 2013-12-27 | Novozymes A/S | Enzymatic reduction of hydroperoxides |
| US11130969B2 (en) | 2013-07-12 | 2021-09-28 | Novozymes, A/S | Direct transfer of polynucleotides between genomes |
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