JPS5554894A - Novel formaldehyde-dehydrogenase and its preparation - Google Patents
Novel formaldehyde-dehydrogenase and its preparationInfo
- Publication number
- JPS5554894A JPS5554894A JP12656878A JP12656878A JPS5554894A JP S5554894 A JPS5554894 A JP S5554894A JP 12656878 A JP12656878 A JP 12656878A JP 12656878 A JP12656878 A JP 12656878A JP S5554894 A JPS5554894 A JP S5554894A
- Authority
- JP
- Japan
- Prior art keywords
- formaldehyde
- enzyme
- dehydrogenase
- preparation
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
NEW MATERIAL:The novel title enzyme produced by the culturing of bacterial belonging to Pseudomonas genus, and capable of irreversibly producing formic acid by the oxidation of formaldehyde through nicotinamide-adenine dinucletide. Molecular weight, 15,000±1,000; isoelectric point, 5.2±0.1; optimum pH, 7W8.
USE: Reagent for the determination of uric acid in blood.
PROCESS: Bacteria belonging to Pseudomonas genus and capable of producing the above enzyme, e.g. Pseudomonas aeruginosa IFO3445, are inoculated in a medium containing 0.05W1wt% of metabolic precursors such as sarcosine, betaine, methylamine, creatinine, formaldehyde, etc., and cultured at 15W40°C and pH6W8 for 1W2 days. The title enzyme is extracted from the medium and purified by salting- out with ammonium sulfate, ion exchange chromatography, etc.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12656878A JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12656878A JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6740579A Division JPS5552944A (en) | 1979-05-30 | 1979-05-30 | Novel quantitizing method of formaldehyde with formaldehyde dehydrogenase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5554894A true JPS5554894A (en) | 1980-04-22 |
JPS6155946B2 JPS6155946B2 (en) | 1986-11-29 |
Family
ID=14938376
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12656878A Granted JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5554894A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60186297A (en) * | 1984-03-06 | 1985-09-21 | Oriental Yeast Co Ltd | Removal of nad+ activation inhibitor |
-
1978
- 1978-10-13 JP JP12656878A patent/JPS5554894A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60186297A (en) * | 1984-03-06 | 1985-09-21 | Oriental Yeast Co Ltd | Removal of nad+ activation inhibitor |
JPH0452117B2 (en) * | 1984-03-06 | 1992-08-20 | Oriental Yeast Co Ltd |
Also Published As
Publication number | Publication date |
---|---|
JPS6155946B2 (en) | 1986-11-29 |
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