JPS56124380A - Preparation of 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductase - Google Patents
Preparation of 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductaseInfo
- Publication number
- JPS56124380A JPS56124380A JP2685480A JP2685480A JPS56124380A JP S56124380 A JPS56124380 A JP S56124380A JP 2685480 A JP2685480 A JP 2685480A JP 2685480 A JP2685480 A JP 2685480A JP S56124380 A JPS56124380 A JP S56124380A
- Authority
- JP
- Japan
- Prior art keywords
- hexadienoic acid
- oxo
- hydroxy
- substituted
- formula
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To obtain an enzyme capable of reducing an aromatic 2,4-hexadienoic acid, by cultivating a microorganism belonging to the genus Pseudomonas, and separating a 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid from the resultant produced microbial cell.
CONSTITUTION: A microorganism, e.g. Pseudomonas cruciviae S93B1 (FERM-P No. 5426) is cultivated in a broth culture medium containing glucose, etc. at pH 4W9 for 10W30hr aerovically, and then a 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductase is separated and purified from the microbial cell by the ultrasonic treatment, fractionation with ammonium sulfate, ion exchange chromatography, etc. The resultant enzyme has a wide substrate specificity to reduce a compound expressed by formula I into a compound expressed by formula II and is capable of reducing even 2,4-hexadienoic acid regardless of natural or unnatural origin. The formed compound is used as an intermediate for medicines and agricultural chemicals.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2685480A JPS56124380A (en) | 1980-03-04 | 1980-03-04 | Preparation of 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2685480A JPS56124380A (en) | 1980-03-04 | 1980-03-04 | Preparation of 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS56124380A true JPS56124380A (en) | 1981-09-30 |
Family
ID=12204854
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2685480A Pending JPS56124380A (en) | 1980-03-04 | 1980-03-04 | Preparation of 2-hydroxy-6-oxo-6-substituted-2,4-hexadienoic acid reductase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56124380A (en) |
-
1980
- 1980-03-04 JP JP2685480A patent/JPS56124380A/en active Pending
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