JPS54129187A - Preparation of choline oxidase by fermentation - Google Patents
Preparation of choline oxidase by fermentationInfo
- Publication number
- JPS54129187A JPS54129187A JP3534978A JP3534978A JPS54129187A JP S54129187 A JPS54129187 A JP S54129187A JP 3534978 A JP3534978 A JP 3534978A JP 3534978 A JP3534978 A JP 3534978A JP S54129187 A JPS54129187 A JP S54129187A
- Authority
- JP
- Japan
- Prior art keywords
- choline
- oxidase
- fermentation
- brevibacterium
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To prepare choline.oxidase by fermentation, using specific microbials capable of producing choline.oxidase.
CONSTITUTION: Choline.oxidase-producing bacteria belonging to Brevibacterium genus, e.g. Brevibacterium album KY 4319, Brevibacterium cerinum KY 4320, etc., are cultured in a nutrient medium containing a carbon source such as glucose, a nitrogen source such as peptone, inorganic salts such as potassium phosphate, and if necessary a minor nutrient such as biotin, at 25W35°C and pH7.0W8.5 for 1W3 days, under shaking or under agitation and aeration, and the accumulated choline.oxidase is separated from the medium. Addition of 0.1W10.0 g/dl of choline to the medium increases the yield of choline.oxidase.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3534978A JPS54129187A (en) | 1978-03-29 | 1978-03-29 | Preparation of choline oxidase by fermentation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3534978A JPS54129187A (en) | 1978-03-29 | 1978-03-29 | Preparation of choline oxidase by fermentation |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54129187A true JPS54129187A (en) | 1979-10-06 |
Family
ID=12439375
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3534978A Pending JPS54129187A (en) | 1978-03-29 | 1978-03-29 | Preparation of choline oxidase by fermentation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54129187A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0356232A2 (en) * | 1988-08-26 | 1990-02-28 | Takeda Chemical Industries, Ltd. | Novel choline oxidase and method for producing the same |
-
1978
- 1978-03-29 JP JP3534978A patent/JPS54129187A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0356232A2 (en) * | 1988-08-26 | 1990-02-28 | Takeda Chemical Industries, Ltd. | Novel choline oxidase and method for producing the same |
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