JPS54129187A - Preparation of choline oxidase by fermentation - Google Patents

Preparation of choline oxidase by fermentation

Info

Publication number
JPS54129187A
JPS54129187A JP3534978A JP3534978A JPS54129187A JP S54129187 A JPS54129187 A JP S54129187A JP 3534978 A JP3534978 A JP 3534978A JP 3534978 A JP3534978 A JP 3534978A JP S54129187 A JPS54129187 A JP S54129187A
Authority
JP
Japan
Prior art keywords
choline
oxidase
fermentation
brevibacterium
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3534978A
Other languages
Japanese (ja)
Inventor
Toru Nakanishi
Yozo Machida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Priority to JP3534978A priority Critical patent/JPS54129187A/en
Publication of JPS54129187A publication Critical patent/JPS54129187A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: To prepare choline.oxidase by fermentation, using specific microbials capable of producing choline.oxidase.
CONSTITUTION: Choline.oxidase-producing bacteria belonging to Brevibacterium genus, e.g. Brevibacterium album KY 4319, Brevibacterium cerinum KY 4320, etc., are cultured in a nutrient medium containing a carbon source such as glucose, a nitrogen source such as peptone, inorganic salts such as potassium phosphate, and if necessary a minor nutrient such as biotin, at 25W35°C and pH7.0W8.5 for 1W3 days, under shaking or under agitation and aeration, and the accumulated choline.oxidase is separated from the medium. Addition of 0.1W10.0 g/dl of choline to the medium increases the yield of choline.oxidase.
COPYRIGHT: (C)1979,JPO&Japio
JP3534978A 1978-03-29 1978-03-29 Preparation of choline oxidase by fermentation Pending JPS54129187A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3534978A JPS54129187A (en) 1978-03-29 1978-03-29 Preparation of choline oxidase by fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3534978A JPS54129187A (en) 1978-03-29 1978-03-29 Preparation of choline oxidase by fermentation

Publications (1)

Publication Number Publication Date
JPS54129187A true JPS54129187A (en) 1979-10-06

Family

ID=12439375

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3534978A Pending JPS54129187A (en) 1978-03-29 1978-03-29 Preparation of choline oxidase by fermentation

Country Status (1)

Country Link
JP (1) JPS54129187A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0356232A2 (en) * 1988-08-26 1990-02-28 Takeda Chemical Industries, Ltd. Novel choline oxidase and method for producing the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0356232A2 (en) * 1988-08-26 1990-02-28 Takeda Chemical Industries, Ltd. Novel choline oxidase and method for producing the same

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