JPS5515004A - High-sensitive and simple coloring calculation method of aceto-acetic acid in blood - Google Patents

High-sensitive and simple coloring calculation method of aceto-acetic acid in blood

Info

Publication number
JPS5515004A
JPS5515004A JP8680378A JP8680378A JPS5515004A JP S5515004 A JPS5515004 A JP S5515004A JP 8680378 A JP8680378 A JP 8680378A JP 8680378 A JP8680378 A JP 8680378A JP S5515004 A JPS5515004 A JP S5515004A
Authority
JP
Japan
Prior art keywords
aceto
acetic acid
blood
azo compound
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP8680378A
Other languages
Japanese (ja)
Other versions
JPS5844210B2 (en
Inventor
Yukio Shigeta
Masaru Harano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanwa Kagaku Kenkyusho Co Ltd
Original Assignee
Sanwa Kagaku Kenkyusho Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanwa Kagaku Kenkyusho Co Ltd filed Critical Sanwa Kagaku Kenkyusho Co Ltd
Priority to JP8680378A priority Critical patent/JPS5844210B2/en
Publication of JPS5515004A publication Critical patent/JPS5515004A/en
Publication of JPS5844210B2 publication Critical patent/JPS5844210B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To calculate aceto-acetic acid in blood with a high sensitivity simply by making aceto-acetic acid in blood into an azo compound by reaction on p-nitropheny diazonium fluoborate and converting this azo compound to a hydrazo compound and comparing color.
CONSTITUTION: Albuminous substances are eliminated from the blood serum of a tested person, and aceto-acetic acid is made into an azo compound by reaction on p-nitrophenyl diazonium fluoborate in citric acid buffer liquid of pH 4.5. Next, phosphorous acid is added to decompose an excess diazonium salt and convert the azo compound to a hydrazo compound, and this solution has the photo absorptivity measured by a wave length of 390nm, and the measured value is compared with a standard calculation line, thereby calculating aceto-acetic acid in blood. In the figure, the standard calculation line aceto-acetic acid is shown and indicates a clear linearity.
COPYRIGHT: (C)1980,JPO&Japio
JP8680378A 1978-07-18 1978-07-18 Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood Expired JPS5844210B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8680378A JPS5844210B2 (en) 1978-07-18 1978-07-18 Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8680378A JPS5844210B2 (en) 1978-07-18 1978-07-18 Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood

Publications (2)

Publication Number Publication Date
JPS5515004A true JPS5515004A (en) 1980-02-01
JPS5844210B2 JPS5844210B2 (en) 1983-10-01

Family

ID=13896949

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8680378A Expired JPS5844210B2 (en) 1978-07-18 1978-07-18 Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood

Country Status (1)

Country Link
JP (1) JPS5844210B2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4976522A (en) * 1988-05-02 1990-12-11 Olympus Optical Co., Ltd. Objective lens system for endoscopes
US6618205B2 (en) 2001-05-14 2003-09-09 Pentax Corporation Endoscope objective optical system

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6452905U (en) * 1987-09-30 1989-03-31

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4976522A (en) * 1988-05-02 1990-12-11 Olympus Optical Co., Ltd. Objective lens system for endoscopes
US6618205B2 (en) 2001-05-14 2003-09-09 Pentax Corporation Endoscope objective optical system

Also Published As

Publication number Publication date
JPS5844210B2 (en) 1983-10-01

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