JPS5783295A - Determination of vitamin b2 by enzyme - Google Patents

Determination of vitamin b2 by enzyme

Info

Publication number
JPS5783295A
JPS5783295A JP15727980A JP15727980A JPS5783295A JP S5783295 A JPS5783295 A JP S5783295A JP 15727980 A JP15727980 A JP 15727980A JP 15727980 A JP15727980 A JP 15727980A JP S5783295 A JPS5783295 A JP S5783295A
Authority
JP
Japan
Prior art keywords
vitamin
test sample
electron acceptor
enzyme
reacted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15727980A
Other languages
Japanese (ja)
Other versions
JPH0146116B2 (en
Inventor
Kiyoshi Tachibana
Masanori Oka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP15727980A priority Critical patent/JPS5783295A/en
Publication of JPS5783295A publication Critical patent/JPS5783295A/en
Publication of JPH0146116B2 publication Critical patent/JPH0146116B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine vitamin B2 in a test sample quickly with saving energy, by reacting the test sample with an enzyme to oxidize vitamin B2 at its 5'- position and an electron acceptor, followed by measuring the formed reduction type electron acceptor.
CONSTITUTION: A test sample is reacted with an enzyme obtained form Schizophyllum commune to oxideze vitamin B2 at its 5'-position, and an electron acceptor, e.g., 2,6-dichlorophenolindolephenol, Methylene Blue, phenazine methosulfate, etc. After they are reacted in a pH of about 4W8.5 at about 20W50°C for about 5minW48hr, the prepared reduction type electron acceptor is measured directly or after it is introduced to other coloring system, to determine an amount of B2 in the test sample.
COPYRIGHT: (C)1982,JPO&Japio
JP15727980A 1980-11-08 1980-11-08 Determination of vitamin b2 by enzyme Granted JPS5783295A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15727980A JPS5783295A (en) 1980-11-08 1980-11-08 Determination of vitamin b2 by enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15727980A JPS5783295A (en) 1980-11-08 1980-11-08 Determination of vitamin b2 by enzyme

Publications (2)

Publication Number Publication Date
JPS5783295A true JPS5783295A (en) 1982-05-25
JPH0146116B2 JPH0146116B2 (en) 1989-10-05

Family

ID=15646177

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15727980A Granted JPS5783295A (en) 1980-11-08 1980-11-08 Determination of vitamin b2 by enzyme

Country Status (1)

Country Link
JP (1) JPS5783295A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102019107462A1 (en) * 2019-03-22 2020-09-24 Ssi Schäfer Automation Gmbh System and method for the automated checking of the functional status of components of a transport network in an intralogistics system
WO2020235525A1 (en) * 2019-05-22 2020-11-26 アンリツインフィビス株式会社 Test body, and diagnosis system and article inspection device using same

Also Published As

Publication number Publication date
JPH0146116B2 (en) 1989-10-05

Similar Documents

Publication Publication Date Title
JPS5592698A (en) Test strip for glucose determination
CA2160850A1 (en) Apparatus for testing lumber stiffness
JPS51114985A (en) Mothod to analyse urine, etc.
JPS53145620A (en) Hue extractor
DK158995C (en) MIXTURE TO DETERMINE THE PRESENCE OF A COMPOUND IN A SAMPLE AND TEST STRIP IMPROVED WITH THE MIXTURE
JPS57144996A (en) Film for quantitative analysis
JPS56117798A (en) Kit for measuring lipoid peroxide
JPS5783295A (en) Determination of vitamin b2 by enzyme
ES2163890T3 (en) PROCEDURE AND DEVICE FOR THE IDENTIFICATION OF ACTIVE SUBSTANCES.
IE42572L (en) Process for enzymatic analysis
IE45395L (en) Serum amylase assay
JPS5648898A (en) Measurement of activity of angiotensinase
JPS55156865A (en) Organism component measuring compound and its manufacture plus organism component measuring composition, organism component measuring method and organism component measuring kit
JPS5635998A (en) Measurement of amylase activity
JPS5648899A (en) Measurement of activity of angiotensinase
DE68919347T2 (en) MEASURING METHOD OF FRESHNESS AND MEASURING SET THEREFOR.
JPS5633545A (en) Method of quantitatively analyzing acid and total iron ion
JPS55146040A (en) Measuring method of concentration
JPS53122482A (en) Gas analyzer
JPS5614157A (en) Fluorescent substrate and measuring method using it
JPS5637556A (en) Quantitative determination method of hydrogen peroxide
JPS57110197A (en) Determination of hydrogen peroxide
JPS5536759A (en) Measuring method of kallikrein in human urine
JPS6420099A (en) Method for determining free fatty acid and reagent composition for determining free fatty acid useful in said determination
JPS56164796A (en) Measurement of activity of gamma-glutamyltranspeptidase