JPS54154592A - Production of enzyme-fixed mold cells - Google Patents
Production of enzyme-fixed mold cellsInfo
- Publication number
- JPS54154592A JPS54154592A JP6144778A JP6144778A JPS54154592A JP S54154592 A JPS54154592 A JP S54154592A JP 6144778 A JP6144778 A JP 6144778A JP 6144778 A JP6144778 A JP 6144778A JP S54154592 A JPS54154592 A JP S54154592A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- enzyme
- production
- fixed mold
- fix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: A yeast, rigonopsis variabilis, is specifically treated to fix the enzyme in the cells without reduction of the activity and the resulting cells are industrially used.
CONSTITUTION: Trigonopsis bariabilis is subjected to activation treatment, such as treatment with a surface active agent, e.g., Triton X-10, or an organic solvent, e.g., toluene and then, the activated cells are brought into contact with glutaldehyde of 0.01W0.5% concentration in a weak alkali medium at 0W50°C within 2 hr to fix the enzyme, D-aminoacid oxidase, in the cells to prepare the objective cells. The resulting cells are used for a catalyst in the oxidative deamination.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6144778A JPS54154592A (en) | 1978-05-23 | 1978-05-23 | Production of enzyme-fixed mold cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6144778A JPS54154592A (en) | 1978-05-23 | 1978-05-23 | Production of enzyme-fixed mold cells |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54154592A true JPS54154592A (en) | 1979-12-05 |
Family
ID=13171316
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6144778A Pending JPS54154592A (en) | 1978-05-23 | 1978-05-23 | Production of enzyme-fixed mold cells |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54154592A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5424196A (en) * | 1990-12-21 | 1995-06-13 | Antibioticos S.P.A. | Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives |
-
1978
- 1978-05-23 JP JP6144778A patent/JPS54154592A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5424196A (en) * | 1990-12-21 | 1995-06-13 | Antibioticos S.P.A. | Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives |
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