JPS54132295A - Preparation of phosphofructokinase - Google Patents
Preparation of phosphofructokinaseInfo
- Publication number
- JPS54132295A JPS54132295A JP3993378A JP3993378A JPS54132295A JP S54132295 A JPS54132295 A JP S54132295A JP 3993378 A JP3993378 A JP 3993378A JP 3993378 A JP3993378 A JP 3993378A JP S54132295 A JPS54132295 A JP S54132295A
- Authority
- JP
- Japan
- Prior art keywords
- thermus
- phosphofructokinase
- disintegrated
- cells
- dispersion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To prepare the title enzyme useful as a bioactive substance, from a dispersion of disintegrated cells obtained by the culturing of bacteria belonging to Thermus genus.
CONSTITUTION: Bacteria belonging to Thermus genus, e.g. highly thermophilic bacteria, Thermus thermophilus HB8, Thermus flavus BS1, AT61, AT62, etc., are cultured in a nutrient medium for about 6 hours, and collected at the latter period of logarithmic phase. The collected cells are disintegrated by, e.g. ultrasonic treatment. The objective phosphofructokinase is separated and purified from the dispersion of the disintegrated cells by centrifugal separation, ammonium sulfate fractionation, column chromatography, etc. The phosphofructokinase thus obtained is an allosteric enzyme, the activity of which is suppressed allosterically with phosphoenol pyruvic acid, and recovered to the original value with adenosine-diphosphoric acid.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3993378A JPS54132295A (en) | 1978-04-05 | 1978-04-05 | Preparation of phosphofructokinase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3993378A JPS54132295A (en) | 1978-04-05 | 1978-04-05 | Preparation of phosphofructokinase |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54132295A true JPS54132295A (en) | 1979-10-15 |
Family
ID=12566736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP3993378A Pending JPS54132295A (en) | 1978-04-05 | 1978-04-05 | Preparation of phosphofructokinase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54132295A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4442214A (en) * | 1979-08-08 | 1984-04-10 | Development Finance Corporation Of New Zealand | Thermo-stable micro-organism |
NL9400836A (en) * | 1994-05-20 | 1996-01-02 | Rijkslandbouwhogeschool | Stable ADP-dependent kinases, and preparation and use thereof |
-
1978
- 1978-04-05 JP JP3993378A patent/JPS54132295A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4442214A (en) * | 1979-08-08 | 1984-04-10 | Development Finance Corporation Of New Zealand | Thermo-stable micro-organism |
NL9400836A (en) * | 1994-05-20 | 1996-01-02 | Rijkslandbouwhogeschool | Stable ADP-dependent kinases, and preparation and use thereof |
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