JPS54101485A - Preparation of novel nuclease - Google Patents
Preparation of novel nucleaseInfo
- Publication number
- JPS54101485A JPS54101485A JP12711878A JP12711878A JPS54101485A JP S54101485 A JPS54101485 A JP S54101485A JP 12711878 A JP12711878 A JP 12711878A JP 12711878 A JP12711878 A JP 12711878A JP S54101485 A JPS54101485 A JP S54101485A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- extract
- bacillus sphaericus
- endonuclease
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare a nuclease having substrate specificity, which discriminates the DNA of specific species of organisms, and breaks the phosphodiester bond at a specific position of the DNA molecule, by separating and purifying an extract of bacterial cells obtained by the culture of bacteria belonging to Bacillus sphaericus.
CONSTITUTION: Endonuclease-producing bacteria belonging to bacillus sphaericus, e.g. Bacillus sphaericus IAM 1286 strain, are cultured in a nutrient medium at 30W37°C using an aerated and agitated fermentor. The cultured cells are collected between the logarithmic growth phase and the initial stage of the stationary phase, disintegrated and extracted to obtain a cell-free extract. The extract is purified by the combination of removal of nucleic acid, ammonium sulfate fractionation, ion exchange chromatography, gel filtration, etc. to obtain the objective endonuclease. The enzyme breaks the phosphodiester bond of Bacillus phage ϕ105C DNA and coil phage λ DNA to DNA fractions having specific lengths.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12711878A JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12711878A JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8788376A Division JPS5315491A (en) | 1976-07-23 | 1976-07-23 | Preparation of novel nucleicacidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54101485A true JPS54101485A (en) | 1979-08-10 |
JPS5625110B2 JPS5625110B2 (en) | 1981-06-10 |
Family
ID=14952042
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12711878A Granted JPS54101485A (en) | 1978-10-16 | 1978-10-16 | Preparation of novel nuclease |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54101485A (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS50121671A (en) * | 1974-03-07 | 1975-09-23 |
-
1978
- 1978-10-16 JP JP12711878A patent/JPS54101485A/en active Granted
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS50121671A (en) * | 1974-03-07 | 1975-09-23 |
Also Published As
Publication number | Publication date |
---|---|
JPS5625110B2 (en) | 1981-06-10 |
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