JPS54101482A - Preparation of novel nuclease - Google Patents

Preparation of novel nuclease

Info

Publication number
JPS54101482A
JPS54101482A JP12711578A JP12711578A JPS54101482A JP S54101482 A JPS54101482 A JP S54101482A JP 12711578 A JP12711578 A JP 12711578A JP 12711578 A JP12711578 A JP 12711578A JP S54101482 A JPS54101482 A JP S54101482A
Authority
JP
Japan
Prior art keywords
dna
endonuclease
extract
bacillus cereus
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12711578A
Other languages
Japanese (ja)
Other versions
JPS5625107B2 (en
Inventor
Tadahiko Ando
Takehiko Shibata
Shiyukuko Igawa
Tetsuo Kin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN Institute of Physical and Chemical Research
Original Assignee
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RIKEN Institute of Physical and Chemical Research filed Critical RIKEN Institute of Physical and Chemical Research
Priority to JP12711578A priority Critical patent/JPS54101482A/en
Publication of JPS54101482A publication Critical patent/JPS54101482A/en
Publication of JPS5625107B2 publication Critical patent/JPS5625107B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare an endonuclease having high substrate specificity, which discriminates the DNA of specific species of organisms, and breaks the phosphodiester bond at a specific position of the DNA molecule, by fractionating an extract of bacterial cells obtained by the culture of a bacteria belonging to Bacillus cereus.
CONSTITUTION: Endonuclease-producing bacteria, belonging to Bacillus cereus, e.g. Bacillus cereus ATCC 14579 are cultured in a nutrient medium at 30W37°C using an aerated and agitated fermentor. The cultured cells are collected between the logarithmic growth phase and the beginning of the stationary phase, disintegrated, and extracted to obtain a cell-free extract. The extract is purified by the combination of removal of nucleic acid, ammonium sulfate fractionation, ion exchange chromatography, gel filtration, etc., to obtain the objective endonuclease. The enzyme breaks the phosphodiester bonds of Bacillus phage ϕ105C DNA and Coli phage λ DNA to DNA fractions having specific length, and is useful as a biological reagent for determination of the structure and functions of gene DNA, and as an agent for the artificial conversion of a gene.
COPYRIGHT: (C)1979,JPO&Japio
JP12711578A 1978-10-16 1978-10-16 Preparation of novel nuclease Granted JPS54101482A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12711578A JPS54101482A (en) 1978-10-16 1978-10-16 Preparation of novel nuclease

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12711578A JPS54101482A (en) 1978-10-16 1978-10-16 Preparation of novel nuclease

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP8788376A Division JPS5315491A (en) 1976-07-23 1976-07-23 Preparation of novel nucleicacidase

Publications (2)

Publication Number Publication Date
JPS54101482A true JPS54101482A (en) 1979-08-10
JPS5625107B2 JPS5625107B2 (en) 1981-06-10

Family

ID=14951970

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12711578A Granted JPS54101482A (en) 1978-10-16 1978-10-16 Preparation of novel nuclease

Country Status (1)

Country Link
JP (1) JPS54101482A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0248561Y2 (en) * 1985-11-27 1990-12-20

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50121671A (en) * 1974-03-07 1975-09-23

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50121671A (en) * 1974-03-07 1975-09-23

Also Published As

Publication number Publication date
JPS5625107B2 (en) 1981-06-10

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