JPH11511493A - 硫酸化多糖類を得る方法 - Google Patents
硫酸化多糖類を得る方法Info
- Publication number
- JPH11511493A JPH11511493A JP9509916A JP50991697A JPH11511493A JP H11511493 A JPH11511493 A JP H11511493A JP 9509916 A JP9509916 A JP 9509916A JP 50991697 A JP50991697 A JP 50991697A JP H11511493 A JPH11511493 A JP H11511493A
- Authority
- JP
- Japan
- Prior art keywords
- concentration
- free radical
- depolymerization
- reaction mixture
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. a)カッソウ綱から得られる粗製フカンを5〜100mg/ml濃度で含有する反 応混合物のV1量に、金属触媒の存在下、5〜30%濃度の過酸化水素溶液のV2量を 、連続的にかつ 0.5〜10時間のあいだ撹拌下に1分当たり V1/1000〜V1/10の割 合で添加し、反応混合物を水酸化ナトリウムを連続的に加えて6〜8のpHかつ40 〜70℃の温度に保持し、 b)この解重合により得られる多糖類を回収すること を特徴とする、フリーラジカル解重合により硫酸化多糖類を得る方法。 2. 粗製フカンが、反応混合物に10〜50mg/mlの濃度で存在することを特徴と する請求項1記載の方法。 3. 5〜20%の濃度かつ V1/50〜V1/500の割合で過酸化水素溶液を用いること を特徴とする請求項1および2いずれかに記載の方法。 4. 金属触媒が、反応混合物に0.01〜0.1Mの濃度で存在することを特徴とする 請求項1〜3のいずれか1つに記載の方法。 5. クロマトグラフィーが DEAE-セファロースCL6Bカラムで行われる際に、フ リーラジカル解重合の前か後のどちらかに行うことができ、その終わりに、0.8M より大きく、好ましくは1Mより大きいNaCl濃度に相当するイオン強度で溶出さ れる画分を回収するイオン交換クロマトグラフィー工程をさらに含むことを特徴 とする請求項1〜4のいずれか1つに記載の方法。 6. 請求項1〜5のいずれか1つに記載のフリーラジカル解重合の方法により 得ることができることを特徴とする10,000g/モルより低いかそれに等しい分子量 の多糖類画分。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9510045A FR2738009B1 (fr) | 1995-08-24 | 1995-08-24 | Procede d'obtention de polysaccharides sulfates |
FR95/10045 | 1995-08-24 | ||
PCT/FR1996/001314 WO1997008206A1 (fr) | 1995-08-24 | 1996-08-23 | Procede d'obtention de polysaccharides sulfates |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11511493A true JPH11511493A (ja) | 1999-10-05 |
JP4074902B2 JP4074902B2 (ja) | 2008-04-16 |
Family
ID=9482059
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50991697A Expired - Fee Related JP4074902B2 (ja) | 1995-08-24 | 1996-08-23 | 硫酸化多糖類を得る方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US6028191A (ja) |
EP (1) | EP0846129B1 (ja) |
JP (1) | JP4074902B2 (ja) |
DE (1) | DE69602369T2 (ja) |
FR (1) | FR2738009B1 (ja) |
WO (1) | WO1997008206A1 (ja) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2772618B1 (fr) * | 1997-12-18 | 2000-02-18 | Ifremer | Utilisation de fucane comme regulateur de la reconstruction des tissus conjonctifs |
FR2797768B1 (fr) * | 1999-09-01 | 2003-06-13 | Ifremer | Utilisation d'un polysaccharide sulfate de bas poids moleculaire pour l'obtention d'un medicament actif contre la thrombose vasculaire |
KR100369518B1 (ko) * | 2000-02-02 | 2003-01-30 | 김공수 | 저분자 다당류의 제조방법 |
ES2266188T3 (es) | 2000-03-27 | 2007-03-01 | Genetics Institute, Llc | Procedimientos de purificacion de proteinas altamente anionicas. |
DK1714982T3 (da) * | 2001-06-05 | 2009-05-04 | Genetics Inst Llc | Fremgangsmåde til rensning af stærkt anioniske proteiner |
EP1306389B1 (en) | 2001-10-24 | 2004-12-29 | Takara Bio Inc. | Sulfated fucan oligosaccharide |
US20040087543A1 (en) * | 2002-04-25 | 2004-05-06 | Zachary Shriver | Methods and products for mucosal delivery |
US20070036821A1 (en) * | 2004-03-05 | 2007-02-15 | Daniels Bruce A | Seaweed extract composition for retardation of cardiovascular disorders and preservation of healthy cardiovascular function |
KR100729638B1 (ko) | 2005-05-20 | 2007-06-19 | 배태진 | 미역포자엽으로부터 분리한 항혈액응고활성을 가진황산화푸코스다당 및 그 제조방법 |
US20070027107A1 (en) * | 2005-07-29 | 2007-02-01 | Curt Hendrix | Compositions and methods for treating estrogen-dependent diseases and conditions |
WO2007019554A2 (en) * | 2005-08-08 | 2007-02-15 | Momenta Pharmaceuticals, Inc. | Polysaccharides for delivery of active agents |
FR2897538B1 (fr) | 2006-02-23 | 2008-05-02 | Univ Paris Descartes | Utilisation de fucanes a des fins de greffe, d'ingenierie et de regeneration osseuses |
WO2010086696A1 (en) * | 2009-01-28 | 2010-08-05 | Therapol | Low-molecular-weight sulphated polysaccharides as candidates for anti-angiogenic therapy |
US20120093725A1 (en) | 2009-04-10 | 2012-04-19 | Inserm (Institut National De La Sante Et De La Recherche Medicale | Fucoidans as Ligands for the Diagnosis of Degenerative Pathologies |
CN101735336B (zh) * | 2009-11-06 | 2012-07-18 | 深圳海王药业有限公司 | 低聚岩藻糖化糖胺聚糖及其制备方法 |
EP2976099B1 (en) | 2013-03-21 | 2018-08-01 | Institut National de la Sante et de la Recherche Medicale (INSERM) | Aminofucoidan as a vector for fibrinolysis in thrombotic diseases |
US20240207190A1 (en) | 2020-06-09 | 2024-06-27 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Fucoidan-functionalized polysaccharide particles with t-pa for targeted thrombolytic therapy |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4552675A (en) * | 1983-08-19 | 1985-11-12 | Fmc Corporation | Composition and method for treating a subterranean formation |
IT1214609B (it) * | 1985-05-17 | 1990-01-18 | Opocrin Spa | Esosaminoglicani solfati depolimerizzati ad attivita'antitrombotica, fibrinolitica, antinfiammatoria, loro procedimento di preparazione e composizioni farmaceutiche che li contengono. |
FR2648463B1 (fr) * | 1989-06-14 | 1993-01-22 | Inst Fs Rech Expl Mer | Polysaccharides sulfates, agent anticoagulant et agent anticomplementaire obtenus a partir de fucanes d'algues brunes et leur procede d'obtention |
-
1995
- 1995-08-24 FR FR9510045A patent/FR2738009B1/fr not_active Expired - Fee Related
-
1996
- 1996-08-23 DE DE69602369T patent/DE69602369T2/de not_active Expired - Lifetime
- 1996-08-23 US US09/011,750 patent/US6028191A/en not_active Expired - Lifetime
- 1996-08-23 WO PCT/FR1996/001314 patent/WO1997008206A1/fr active IP Right Grant
- 1996-08-23 EP EP96929366A patent/EP0846129B1/fr not_active Expired - Lifetime
- 1996-08-23 JP JP50991697A patent/JP4074902B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US6028191A (en) | 2000-02-22 |
EP0846129B1 (fr) | 1999-05-06 |
FR2738009A1 (fr) | 1997-02-28 |
FR2738009B1 (fr) | 1997-10-31 |
EP0846129A1 (fr) | 1998-06-10 |
DE69602369T2 (de) | 1999-11-18 |
WO1997008206A1 (fr) | 1997-03-06 |
JP4074902B2 (ja) | 2008-04-16 |
DE69602369D1 (de) | 1999-06-10 |
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