JPH11510785A - ヒスチジン部分を有する融合ポリペプチドに作用する抗体 - Google Patents
ヒスチジン部分を有する融合ポリペプチドに作用する抗体Info
- Publication number
- JPH11510785A JPH11510785A JP8525218A JP52521896A JPH11510785A JP H11510785 A JPH11510785 A JP H11510785A JP 8525218 A JP8525218 A JP 8525218A JP 52521896 A JP52521896 A JP 52521896A JP H11510785 A JPH11510785 A JP H11510785A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- histidine
- antibodies
- fusion polypeptide
- amino acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 40
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 39
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 39
- 230000004927 fusion Effects 0.000 title claims abstract description 30
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 11
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 241001465754 Metazoa Species 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 claims description 7
- 230000003053 immunization Effects 0.000 claims description 6
- 238000002649 immunization Methods 0.000 claims description 5
- 238000001262 western blot Methods 0.000 claims description 5
- 238000002965 ELISA Methods 0.000 claims description 4
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 4
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 210000004989 spleen cell Anatomy 0.000 claims description 4
- 238000010166 immunofluorescence Methods 0.000 claims description 3
- 238000001114 immunoprecipitation Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 abstract description 4
- 235000001014 amino acid Nutrition 0.000 description 21
- 150000001413 amino acids Chemical class 0.000 description 21
- 235000014304 histidine Nutrition 0.000 description 17
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 11
- 239000002953 phosphate buffered saline Substances 0.000 description 11
- 241000699670 Mus sp. Species 0.000 description 10
- 239000002671 adjuvant Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 150000002411 histidines Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102100033270 Cyclin-dependent kinase inhibitor 1 Human genes 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 101000944380 Homo sapiens Cyclin-dependent kinase inhibitor 1 Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 240000007711 Peperomia pellucida Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
- C07K16/1054—Lentiviridae, e.g. HIV, FIV, SIV gag-pol, e.g. p17, p24
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- AIDS & HIV (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.ヒスチジン部分を有する融合ポリペプチドに対する抗体。 2.ポリクローナルであることを特徴とする、請求項1記載の抗体。 3.モノクローナルであることを特徴とする、請求項1記載の抗体。 4.DSM(微生物のドイツタイプカルチャーコレクション)でACC 220 7の下で寄託されていることを特徴とする、請求項3記載の抗体。 5.ヒスチジン融合ポリペプチドを用いて動物を免疫し、 (a)ポリクローナル抗体を動物の血清から得る、または (b)モノクローナル抗体を動物の脾臓細胞とミエローマ細胞との融合後に得る ことを特徴とする請求項1〜4いずれか記載の抗体の調製方法。 6.ヒスチジン融合ポリペプチドの混合物を免疫化のために使用することを特徴 とする、請求項5記載の方法。 7.ヒスチジン部分を有する融合ポリペプチドの検出方法における、請求項1〜 4いずれか記載の抗体の使用。 8.検出方法が、ウエスタンブロット、ELISA、免疫蛍光法または免疫沈降 法である請求項7記載の使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19507166.2 | 1995-03-01 | ||
DE19507166A DE19507166C1 (de) | 1995-03-01 | 1995-03-01 | Antikörper gegen ein Histidin-Fusionspolypeptid, das einen Histidin-Anteil aufweist |
PCT/DE1996/000369 WO1996026963A1 (de) | 1995-03-01 | 1996-03-01 | Antikörper gegen ein, einen histidin-anteil aufweisendes fusionspolypeptid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11510785A true JPH11510785A (ja) | 1999-09-21 |
JP3799059B2 JP3799059B2 (ja) | 2006-07-19 |
Family
ID=7755368
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52521896A Expired - Lifetime JP3799059B2 (ja) | 1995-03-01 | 1996-03-01 | ヒスチジン部分を有する融合ポリペプチドに作用する抗体 |
Country Status (9)
Country | Link |
---|---|
US (3) | US6790940B1 (ja) |
EP (1) | EP0815141B2 (ja) |
JP (1) | JP3799059B2 (ja) |
AT (1) | ATE207935T1 (ja) |
CA (1) | CA2217186A1 (ja) |
DE (2) | DE19507166C1 (ja) |
DK (1) | DK0815141T3 (ja) |
ES (1) | ES2166878T3 (ja) |
WO (1) | WO1996026963A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013017423A (ja) * | 2011-07-11 | 2013-01-31 | Yamaguchi Univ | 抗ヒスチジンタグ抗体 |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19507166C1 (de) | 1995-03-01 | 1996-04-18 | Deutsches Krebsforsch | Antikörper gegen ein Histidin-Fusionspolypeptid, das einen Histidin-Anteil aufweist |
DE19728697C1 (de) * | 1997-07-04 | 1999-03-25 | Deutsches Krebsforsch | Humaner Antikörper gegen ein Fusions(poly)peptid bzw. -protein, das einen Anteil von mindestens sechs Histidinen aufweist |
US7794946B1 (en) | 1998-02-04 | 2010-09-14 | Life Technologies Corporation | Microarray and uses therefor |
DE69922365T2 (de) * | 1998-04-30 | 2005-04-14 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Neue methode zur selektion von klonen einer expressionsbibliothek mittels "rearraying" |
US7294504B1 (en) | 2001-12-27 | 2007-11-13 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods and compositions for DNA mediated gene silencing |
EP1587907B1 (en) | 2003-01-07 | 2011-03-02 | Dyax Corporation | Kunitz domain library |
SG187787A1 (en) | 2010-08-10 | 2013-03-28 | Amgen Inc | Dual function in vitro target binding assay for the detection of neutralizing antibodies against target antibodies |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5130416A (en) | 1986-08-13 | 1992-07-14 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant DNA clone containing a genomic fragment of PfHRP-II gene from plasmodium falciparum |
JPH07501711A (ja) * | 1992-09-30 | 1995-02-23 | ドイチェス クレブスフォーシュングスツェントラム スチフトゥング デス エフェントリヒェン レヒツ | p53特異的抗体の検出法 |
DE4339533C2 (de) * | 1993-11-19 | 1997-07-03 | Deutsches Krebsforsch | Nachweisverfahren für hdm-2 spezifische Antikörper |
US5840834A (en) * | 1994-12-21 | 1998-11-24 | Virginia Commonwealth University | Technique for joining amino acid sequences and novel composition useful in immunoassays |
DE19507166C1 (de) * | 1995-03-01 | 1996-04-18 | Deutsches Krebsforsch | Antikörper gegen ein Histidin-Fusionspolypeptid, das einen Histidin-Anteil aufweist |
-
1995
- 1995-03-01 DE DE19507166A patent/DE19507166C1/de not_active Expired - Lifetime
-
1996
- 1996-03-01 EP EP96904728A patent/EP0815141B2/de not_active Expired - Lifetime
- 1996-03-01 US US08/913,139 patent/US6790940B1/en not_active Expired - Lifetime
- 1996-03-01 AT AT96904728T patent/ATE207935T1/de not_active IP Right Cessation
- 1996-03-01 WO PCT/DE1996/000369 patent/WO1996026963A1/de active IP Right Grant
- 1996-03-01 DK DK96904728T patent/DK0815141T3/da active
- 1996-03-01 DE DE59608073T patent/DE59608073D1/de not_active Expired - Fee Related
- 1996-03-01 ES ES96904728T patent/ES2166878T3/es not_active Expired - Lifetime
- 1996-03-01 JP JP52521896A patent/JP3799059B2/ja not_active Expired - Lifetime
- 1996-03-01 CA CA002217186A patent/CA2217186A1/en not_active Abandoned
-
2003
- 2003-10-14 US US10/686,355 patent/US20040105854A1/en not_active Abandoned
-
2009
- 2009-05-14 US US12/465,855 patent/US7713712B2/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013017423A (ja) * | 2011-07-11 | 2013-01-31 | Yamaguchi Univ | 抗ヒスチジンタグ抗体 |
Also Published As
Publication number | Publication date |
---|---|
EP0815141A1 (de) | 1998-01-07 |
EP0815141B1 (de) | 2001-10-31 |
DE19507166C1 (de) | 1996-04-18 |
US20090221040A1 (en) | 2009-09-03 |
ATE207935T1 (de) | 2001-11-15 |
JP3799059B2 (ja) | 2006-07-19 |
US6790940B1 (en) | 2004-09-14 |
DK0815141T3 (da) | 2002-01-21 |
CA2217186A1 (en) | 1996-09-06 |
WO1996026963A1 (de) | 1996-09-06 |
ES2166878T3 (es) | 2002-05-01 |
US20040105854A1 (en) | 2004-06-03 |
US7713712B2 (en) | 2010-05-11 |
DE59608073D1 (de) | 2001-12-06 |
EP0815141B2 (de) | 2009-02-25 |
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