JPH1135429A - Grey hair-proofing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition by formulating plant extract(s) as active ingredient. SOLUTION: This composition, which is intended to promote tyrosinase activity, is obtained by formulating, as active ingredient, at least one kind of extract derived from a plant belonging to the genus Compositae Eclipta (e.g. Eclipta alba Hassk). This composition may contain an ingredient (e.g. swertia exract, carrot extract) usable as a hair tonic ingredient. This composition has only to be in such a form as to be applicable to the skin or scalp such as a liquid, milky lotion or ointment, being in the form of emollient lotion, emollient cream, moisture gel, etc. The optimum content of the plant extract(s) in the composition is pref. 0.01-5 wt.% on a dry basis. When this composition is to be applied to the scalp, the daily dose of the active ingredient is e.g. 0.1-80 mg/adult each time.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a composition for promoting tyrosinase activity or a composition for preventing gray hair, comprising a plant extract as an active ingredient.

[0002]

2. Description of the Related Art As a composition for preventing gray hair, an active substance is screened with reference to the mechanism of gray hair development or, conversely, the mechanism of melanin pigment formation, or the effect of various substances on white hair prevention is examined at random. The use of a wide variety of compounds (or substances) obtained by doing so has been proposed.

[0003] For example, those that improve the ability of melanocytes to produce cyclic AMP (see JP-A-4-124122) and those that activate the production of melanocytes by melanocytes (for example, JP-A-5-78222, JP-A-7-222). -285
874, JP-A-7-316026, etc.), and those which are said to exhibit both a hair-growth effect and a gray-hair preventing effect (JP-A-7-112918, JP-A-7-126).
129, etc.). Most of them contain an extract from animals and plants as an active ingredient, and it has been described that a certain purpose can be achieved.

[0004]

However, taking into account the complexity of the mechanism of gray hair development, and focusing on the diversity of natural products, a novel white hair prevention method utilizing further various components derived from natural products is considered. It would also be desirable to provide a composition. Therefore, an object of the present invention is to provide a white hair preventive composition which has not been conventionally used for preventing gray hair, but has an excellent effect in actual use compared to plant components which have been used for preventing gray hair (or proposed to be used). To provide a composition for use.

[0005]

Means for Solving the Problems The present inventors have conducted basic research on the mechanism of gray hair development and the effects of various compounds or substances at each stage, and have repeatedly studied the effectiveness in actual use. . As a result, it was found that a plant extract belonging to the genus Asteraceae (Eclipta), which can be easily and abundantly obtained, exhibits an excellent tyrosinase activity-promoting activity and an effect of preventing gray hair (or blackening gray hair). . The composition for promoting tyrosinase activity can be used not only for preventing gray hair, but also for self-tanning, for example.

Therefore, according to the present invention, there is provided a composition for promoting tyrosinase activity or a composition for preventing gray hair comprising a plant extract as an active ingredient, wherein the active ingredient is Compositae (Eclipta). ) Are provided. In the present invention, the term “preventing gray hair” means
It should be understood that the term "blackening effect of gray hair" is also used to encompass the concept.

Hereinafter, the configuration of the present invention will be described in detail. The plant belonging to the genus Asteraceae (Compositae) belonging to the genus Eclipta (Eclipta) used in the present invention may be any type of plant as long as it belongs to the genus and meets the purpose of the present invention. A rare plant is Takasaburo (scientific name: Ec
lipta alba Hassk). Extracts from these plants can use leaves, stems, flowers, bark, seeds or fruits, whole plants, but preferably whole plants, leaves, roots.

According to the present invention, each extract can be used alone or in combination. The extract used in the present invention is generally obtained by drying each plant, if necessary, then immersing the plant in an extraction solvent for a certain period of time, or contacting it with an extraction solvent that has been heated to reflux, followed by filtration and concentration. Obtainable. The extraction solvent used includes, but is not limited to, water, especially alcohols such as methanol and ethanol, 1,3-butylene glycol, acetone, ethyl acetate and the like, and mixtures thereof.

[0009] The extract thus obtained can be used as a diluent, anionic surfactant, nonionic surfactant, cationic surfactant commonly used in hair compositions or skin external compositions (cosmetics or drugs). Agents, amphoteric surfactants, higher alcohols, oils, humectants, thickeners, solvents, usability improvers, preservatives, antioxidants, sequestering agents, UV inhibitors, powders, other active substances, colors Formulations can be made by appropriately using agents, flavors and the like according to the intended dosage form. The additives used in the present invention are not necessarily separable by a single function, but will be described below in accordance with normally classified examples.

Examples of the diluent include water, ethanol, isopropyl alcohol, and glycols. Examples of the anionic surfactant include alkyl benzene sulfonate, polyoxyalkylene alkyl sulfate, alkyl sulfate, olefin sulfonate, alkyl phosphate, polyoxyalkylene alkyl ether phosphate, and dialkyl sulfosuccinic acid. Salts and fatty acid salts. Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyhydric alcohol fatty acid partial ester, polyoxyethylene polyhydric alcohol fatty acid partial ester, polyglycerin fatty acid ester, and polyoxyethylene cured castor. Examples include oil derivatives and fatty acid diethanolamide. Examples of the cationic surfactant include tertiary aliphatic amine salts, alkyltrimethylammonium halide, dialkyldimethylammonium halide and alkyldimethylbenzylammonium halide. Examples of the amphoteric surfactant include amidobetaine type and imidazo. Examples include surfactants such as a rhinium betaine type and a sulfobetaine type. Examples of the higher alcohol include cetyl alcohol, stearyl alcohol, and behenyl alcohol. Examples of the oil component include higher fatty acids, solid paraffin, liquid paraffin, silicone oil, high molecular silicone and derivatives thereof, squalane, petrolatum, and ester oil. Examples of the humectant include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol and sorbitol. Examples of the thickener include methyl cellulose, hydroxyethyl cellulose, carrageenan,
Examples include carboxymethyl cellulose and cationized cellulose. Examples of the powder include resin powder such as silica, nylon powder and polyethylene powder.

[0011] The composition for preventing gray hair of the present invention may contain any component which can be used as a component of a hair restorer as long as the effect of the present invention is not adversely affected. Such components include active ingredients and auxiliaries which have been suggested to have a hair-growth effect or the like. For example, plant extracts such as assembly extract, carrot extract, vitamin B6, vitamin E and its derivatives, vitamins such as biotin, pantothenic acid and its derivatives, glycyrrhizic acid and its derivatives, nicotinic acid esters such as benzyl nicotinate , Serine, amino acids such as methionine, cepharanthin, capronium chloride, minoxidil, nicorandil, acetylcholine derivatives, cyclosporins, female hormones such as estradiol, and the like, and mixtures thereof. Furthermore, for example, antibacterial agents such as hinokitiol, hexachlorophene, benzalkonium chloride, cetylpyridinium chloride, undecylenic acid, trichlorocarbanilide and bithionol, cooling agents such as menthol, salicylic acid, zinc and its derivatives, lactic acid and its alkyl esters, etc. Active substances, organic acids such as citric acid, and amino acids such as arginine can also be added to the compositions of the present invention.

The composition of the present invention may be in any form as long as it can be applied to the skin or scalp, such as a liquid, an emulsion, an ointment, etc. Generally, emollient lotion, emollient cream, moisture gel, lotion, Hair tonic, hair liquid, scalp treatment,
Products can be in the form of products called hair cream, aerosol mousse, aerosol spray, hair gel, spray mousse, and the like.

[0013] The optimum amount of plant extract to be included in the composition in these product forms cannot be specified because it varies depending on the form, but is generally 0.005 as dry matter per total composition weight. -20% by weight, preferably 0.01-5% by weight. If the amount is less than 0.005% by weight, the object of the present invention may not be sufficiently achieved. If the amount exceeds 20% by weight, it is not preferable in terms of formulation. Taking the application of the composition of the present invention thus prepared to the head as an example, the dose of the active ingredient is 0.001 to 100 mg, preferably 0.1 to 80 mg per day for adults.
Can be applied.

[0014]

Next, the present invention will be described in more detail with reference to examples. The present invention is not limited by these. The compounding amount is% by weight.

Example 1: Preparation of extract 50 g of whole plant (dry matter) of Takasaburo (Eclipta alba Hassk) was soaked in 7.5 L of ethanol at room temperature for 1 week, and the solvent was distilled off from the extract. 2.905 g of extract was obtained (Example 1).

(Effect Confirmation Test) Each of the extracts obtained as described above was added to dimethyl sulfoxide (DMSO) for 1 hour.
%, This solution was diluted to adjust the concentration, and the following test was performed using the obtained diluted solution.

(1) Cell culture method B16 melanoma cultured cells derived from mice were used. 3
The cells were cultured in a CO2 incubator (95% air, 5% carbon dioxide) at 37 ° C in an Eagle MEM medium containing 5% fetal bovine serum (FBS). After 24 hours of cultivation, the test sample solution is adjusted to a final concentration (concentration in terms of dry extract) of 10
^-3 to 10 ^-5 %, and the culture was further continued for 3 days, and the visual evaluation of the amount of melanin production and the effect of promoting tyrosinase activity were measured by the following methods.

(2) Judgment of Melanin Amount Visualization: Place a diffusion plate on the lid of the well plate, observe the amount of melanin in the cells with an inverted microscope, and compare with the sample without the extract (reference). did. Table 1 shows the results.

<Judgment Criteria> +: Black (melanin content is large) ±: Slightly dark (melanin content is slightly high)-: Control (melanin content is hardly recognized)

(3) Measurement of Tyrosinase Activity The medium in the wells was removed, and the wells were washed twice with 100 μl of PBS. To each well was added in PBS containing 1% Triton -X of 45 [mu] l (Rohm and Haas). The plate was shaken for 1 minute to carefully break the cell membrane, and the absorbance at 475 nm was measured with a microplate reader. This was taken as the absorbance at 0 minutes. Then quickly add 5 μl of 1
A 0 mM L-Dopa solution was added, the mixture was transferred to a 37 ° C. incubator, and reacted for 60 minutes. After the reaction, the plate was vibrated for 1 minute, and the absorbance at the time of 60 minutes (475 n
m) was measured. The increase in the absorbance difference of each extract-added sample with respect to the difference between the absorbance at 0 minutes of the sample to which no plant extract was added (control) and at 60 minutes after the addition of dopa was determined.

[0021]

[Table 1] ──────────────────────────────────── Test Melanin production visual evaluation Tyrosinase activity promotion rate (%) ──────────────────────────────────── concentration (wt%) 10 ^-4 10 ^{- 3} 10 ^-2 10 ^-4 10 ^-3 10 ^-2 ──────────────────────────────────── None Addition----------------------------------- ───

(4) Human Prevention of Gray Hair by Cumulative Application (Test Method)
Forty consecutive males and females aged 0 to 60 used the product of the present invention and the comparative example on the left and right scalp twice a day (morning and night) for four consecutive months, and tested the condition of the application site. Comparison was made before and after to examine the effect of preventing and improving white hair.

While applying the lotion containing the active ingredient of the present invention every day, the ratio of preventing the generation of gray hair was determined before and after 4 months from the start of the application.
The number of white hairs per 0 hairs was counted.

(Judgment Criteria) ++ (Excellent effect): 50% or more of testers whose number after application is less than 80% of the number of gray hair before the start of application. + (Effective): White hair before the start of application. 50% or more of testers whose number after application is less than 90% of the number of test pieces ± (slightly effective): 50 testers whose number after application is less than 100% of the number of gray hairs before the start of application. % Or more-(ineffective): less than 50% of testers whose number after application is less than 100% with respect to the number of gray hairs before the start of application. The results are shown in Table 2.

In this test, various whitening preventing lotions were prepared according to the following composition, and the effect of preventing the whitening by cumulative application was examined.

Ingredients% by weight Test substance 1.0 Polyvinylpyrrolidone / vinyl acetate copolymer 5.0 Preservatives Appropriate amount Fragrance Appropriate amount Ethyl alcohol 30.0 Purified water 62.5 Silicone derivative 0.5 Glycerin 2.0 <Production method> A polymer, a preservative, and a fragrance are added to ethyl alcohol and uniformly dissolved.

An aqueous layer (purified water, silicone derivative, glycerin) which has been dissolved in advance is added and dissolved.

Table 2: Degree of Prevention of White Hair When Each Extract is Added Test Substance No Degree of Prevention of White Hair- Takasaburo Extract ++ Other formulation examples of the composition of the present invention are described below.

Formulation Example 1 Hair tonic (1) Hardened castor oil EO (40 mol) adduct 2.0% by weight (2) Takasaburo extract (dry) 0.1 (3) 95% ethanol 70.0 (4) ) Perfume appropriate amount (5) Purified water residue (Preparation method) Add (1), (2) and (4) to (3),
After stirring and dissolving, add (5) and add the title composition (product)
I got

Formulation Example 2 Hair tonic (1) Glycerin 2.0% by weight (2) L-menthol 0.1 (3) Takasaburo extract (dry matter) 0.5 (4) 95% ethanol 60.0 (5) ) Perfume appropriate amount (6) Purified water residue (Preparation method) Add (1), (2), (3), and (5) to (4), dissolve with stirring, and add (6) to the title. (Product) was obtained.

Formulation Example 3 Hair tonic (1) Polyethylene glycol 200 2.0% by weight (2) L-menthol 0.2 (3) Takasaburo extract (dry) 1.0 (4) 95% ethanol 50.0 (5) Appropriate amount of perfume (6) Residual purified water (Preparation method) (1), (2), (3), and (5) were added to (4), and the mixture was stirred and dissolved, and then (6) was added. To give the title composition (product).

Formulation Example 4 Hair Liquid (1) 15.0% by weight of polyoxypropyl butyl ether (40PO) (2) Polyoxypropyl butyl ether phosphoric acid (40PO) 15.0 (3) Takasaburo extract (dry matter) 0 (4) 1,3-butylene glycol 5.0 (5) 95% ethanol 50.0 (6) Appropriate amount of fragrance (7) Dye, edetic acid Appropriate amount (8) Purified water residue Same as in Preparation Example 1 Prepare.

Formulation Example 5 Scalp treatment (1) 0.5% by weight of 1,3-propylene glycol (2) Pentaerythritol tetra-2-ethylhexanate 1.2 (3) 95% ethanol 60.0 (4) Takasaburo Extract (dry matter) 2.0 (5) Assembly extract 1.0 (6) Appropriate amount of perfume (7) DME / LPG (95/5) Residue Prepared in the same manner as in Formulation Example 1.

Formulation Example 6 Hair cream Phase A (1) Liquid paraffin 5.0% by weight (2) Cetostearyl alcohol 5.5 (3) Vaseline 5.5 (4) Glyceryl monostearate 3.0 (5) EO (20 mol addition) -2-octyldodecyl ether 3.0 (6) Vitamin E acetate 0.05 (7) Propyl paraben 0.3 (8) Fragrance 0.05 B phase (9) Takasaburo extract (dry matter) 0.01 (10) Glycerin 7.0 (11) Dipropylene glycol 20.0 (12) Polyoxyethylene glycol 4000 5.0 (13) Sodium hexametaphosphate 0.005 (14) Purified water residue (Preparation method) The phase A was heated and melted and mixed, and the hot melt mixture of the phase B was added thereto and emulsified by a homomixer to obtain the title composition (product).

Formulation Example 7 Hair Gel (1) 0.7% by weight of carboxyvinyl polymer (2) Polyvinylpyrrolidone 2.0 (3) Glycerin 4.0 (4) Takasaburo extract (dry) 0.05 (5) Water Sodium oxide qs (6) ethyl alcohol 20.0 (7) polyoxyethylene octyl dodecyl ether qs (8) flavor, chelating agent (EDTA) qs (9) purified water residue (preparation method) Carboxyvinyl polymer Disperse in glycerin and some purified water. The other components were dissolved in the remaining purified water and added with stirring to obtain the title composition (product).

Formulation Example 8 Water grease (1) Carboxyvinyl polymer 0.5% by weight (2) Glycerin 50.0 (3) Sodium hydroxide proper amount (4) Ethyl alcohol 10.0 (5) Takasaburo extract (dried) 5.0) (6) Polyoxyethylene octyl dodecyl ether qs (7) flavor, chelating agent (EDTA) qs (8) purified water residue (Preparation method) Same as in Formulation Example 5.

Formulation Example 9 Hairspray (stock solution formulation) (1) Acrylic resin alkanolamine solution (50%) 7.0% by weight (2) Cetyl alcohol 0.1 (3) Silicone oil (methylphenylpolysiloxane) 3 (4) Ethyl alcohol 92.6 (5) Takasaburo extract (dry product) 4.0 (6) Appropriate amount of flavor (filling formulation) (7) Stock solution 50.0 (8) Dimethyl ether 45.0 (9) LPG 5.0 (Preparation method) (1), (2) and (3) were uniformly emulsified with a homomixer. This was added to a solution of other components to prepare a stock solution. For filling, fill the can with the undiluted solution, attach the valve,
Filling with gas gave the title composition (product).

Formulation Example 10 Lotion (1) 1,3-butylene glycol 6.0% by weight (2) glycerin 4.0 (3) oleyl alcohol 0.1 (4) POE (20) sorbitan monolaurate 5 (5) POE (15) Lauryl alcohol ether 0.5 (6) Takasaburo extract (dried product) 1.0 (7) Ethanol 10.0 (8) Fragrance, pigment, preservative, anti-fading agent and buffer (9) Purified water residue (Preparation method) (9) (1) and (2) and (8)
Are dissolved at room temperature to obtain an aqueous phase.
In (7), the fragrance of (8), (3), (4), (5) and (6) are dissolved and mixed and solubilized in the aqueous phase. Thereafter, the color was adjusted with the coloring agent of (8) to obtain the title composition (product).

Formulation Example 11 Emollient lotion (1) 2.0% by weight of stearic acid (2) Cetyl alcohol 1.5 (3) Vaseline 4.0 (4) Squalane 5.0 (5) Takasaburo extract (dry matter) 5.0 (6) Glycerol tri-2-ethylhexanoate 2.0 (7) Sorbitan monooleate 2.0 (8) Dipropylene glycol 5.0 (9) PEG 1500 3.0 (10) Triethanol Amine 1.0 (11) Perfume and preservatives Appropriate amount for each (12) Purified water residue (Preparation method) Use a conventional method.

Formulation Example 12 Emollient cream (1) Cetyl alcohol 5.0% by weight (2) Stearic acid 3.0 (3) Vaseline 5.0 (4) Squalane 10.0 (5) Glycerol tri-2-ethylhexanoic acid Ester 7.0 (6) Takasaburo extract (dried) 0.5 (7) Dipropylene glycol 5.0 (8) Glycerin 5.0 (9) Propylene glycol monostearate 8.0 (10) POE ( 20) Cetyl alcohol ether 3.0 (11) Triethanolamine 1.0 (12) Perfume, preservative and antioxidant Appropriate amount for each (13) Purified water residue (Preparation method) Use a conventional method.

Formulation Example 13 Moisture gel (1) 7.0% by weight of dipropylene glycol (2) PEG 1500 8.0 (3) Takasaburo extract (dry) 0.1 (4) Carboxyvinyl polymer 0.4 (5) ) Methylcellulose 0.2 (6) POE (15) Oleyl alcohol ether 1.0 (7) Potassium hydroxide 0.1 (8) Perfume, pigment, preservative, anti-fading agent and chelating agent Appropriate amount for each (9) Purified water Residue (Preparation method) Use a conventional method.

[0042]

According to the present invention, there is provided a composition excellent in tyrosinase activity accelerating action and gray hair preventing action.

Continued on the front page (72) Inventor Yoshiki Moriya 1050 Nippa-cho, Kohoku-ku, Yokohama-shi, Kanagawa Inside the Shiseido Daiichi Research Center (72) Inventor Nao Kojima 7-5-5 Ginza, Chuo-ku, Tokyo Inside Shiseido (72) Inventor Eiji Ifuku 2-12-1 Fukuura Kanazawa-ku, Yokohama-shi, Kanagawa Prefecture Shiseido Second Research Center Co., Ltd.

Claims (3)

[Claims] 1. A composition for promoting tyrosinase activity, comprising a plant extract as an active ingredient, wherein the active ingredient is:
A composition comprising at least one extract derived from a plant belonging to the genus Eclipta (Compositae). 2. A composition for preventing gray hair, comprising a plant extract as an active ingredient, wherein the active ingredient is an Asteraceae (Comp.
ositae) A composition comprising at least one extract from a plant belonging to the genus Eclipta. 3. A plant belonging to the genus Eclipta (Compositae) belonging to the genus Eclipta, wherein the plant is Eclipta (scientific name: Ecli
3. The composition according to claim 1, which is pta alba Hassk).