JP2002114700A - Melanocyte activator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a melanocyte activator preparation containing a melanocyte activator component as an active ingredient by finding the component for activating the melanocyte, and a method for using the component in gray hair prevention or tanning. SOLUTION: A melanocyte activator containing an extract of a plant belonging to Compositae Wedelia such as Wedelia chinensis MERR as an active ingredient is provided and is used as a gray hair preventing agent, a tanning agent, or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a melanocyte activator useful for preventing gray hair and tanning.

[0002]

2. Description of the Related Art Melanocytes, which are melanin-producing cells, constitute 5 to 10% of epithelial cells which form the basal layer of the epidermis.
To a lesser extent, they continue to be scattered in the basal layer, giving melanin to the surrounding epithelial cell population. Depending on the amount of melanin, the color tone of the skin or hair changes from black to white.

[0003] Typically, reduced melanocyte melanin production is a direct cause of gray hair. In addition, it becomes difficult for a person who wants to make the skin black as desired to make the skin black.

[0004] In particular, for gray hair, most people do not like it, and it is desired to provide an effective inhibitor. With respect to anti-grey-hair agents, a variety of anticancer agents have been obtained by screening active substances or randomly examining the anti-gray action of various substances with reference to the mechanism of gray hair development and the mechanism of melanin pigment formation. The use of ingredients has been proposed.

[0005] For example, those that improve the ability of melanocytes to produce cyclic AMP (see JP-A-4-124122) and those that activate the production of melanin by melanocytes (for example, JP-A-5-78222, JP-A-7-222) -285
874, JP-A-7-316026, etc.), and those exhibiting both a hair-growth effect and a gray-hair preventing effect (JP-A-7-112918, JP-A-7-12612).
No. 9).

[0006]

SUMMARY OF THE INVENTION In view of the close relationship between melanin production and gray hair and skin color, the present inventor has found that by activating melanocytes that control melanin production, it is possible to obtain effects such as prevention of gray hair. With this in mind, the objectives of the present invention have been set.

[0007] That is, the problem to be solved by the present invention is to find a component that activates melanocytes, to provide an agent containing this component as an active component, and to provide a way to utilize the same in prevention of gray hair and tanning. is there.

[0008]

Means for Solving the Problems The present inventors have conducted basic research on the mechanism of gray hair development and the effects of various compounds or substances at each stage, and have repeatedly studied the effectiveness in actual use. . As a result, a plant extract belonging to the genus Asteraceae (Compositae), which is easily available in large quantities, is a powerful effect for activating melanocytes, and has excellent cyclic AMP inducing action and tyrosinase activity promoting action. Was found to be able to demonstrate. And it discovered that this plant extract actually showed a gray hair prevention (or blackening of gray hair) effect.

[0009] That is, the present inventor, in the present application, discloses a melanocyte activator (hereinafter also referred to as the present melanocyte activator) containing an extract of a plant belonging to the genus Compositae (Wedelia) as an active ingredient. provide.

The melanocyte activator of the present invention is an agent which can be used as an anti-whitening agent, a tanning agent and the like due to its mechanism of action. In the present invention, “preventing gray hair”
Is literally used to mean not only the prevention of the generation of white hair, but also the action of blackening the once generated white hair.
Similarly, "tanning" is used as a word meaning that the skin is positively blackened.

[0011]

Embodiments of the present invention will be described below. A. Active ingredient of the present melanocyte activator The raw material of the plant extract used as the active ingredient of the melanocyte activator is, as described above, asteraceae (Compositae).
ae) It is a plant belonging to the genus Nekonoshita (Wedelia) (hereinafter sometimes abbreviated as genus Nekonoshita), and as long as it is not particularly limited.

Cats are a perennial or annual plant with coarse hair widely distributed in the tropics of the world, and about 75 species are known. Examples of the species of the genus Nematoda that can be applied to the present invention and are suitable include, for example, yellow horned sesame (scientific name: Wedelia biflora DC.) And crabwood (scientific name:
Wedeliachinensis MERR), cat nosita (scientific name: Wedelia)
Hemsl.) And the like.

[0013] Extracts from these plants include leaves, stems,
Flowers, bark, seeds or fruits, those derived from whole plants can be used, but those derived from whole plants, leaves or roots can be used.
It is preferably used as a raw material for the extract.

The extract of the genus Caterpillar genus used as an active ingredient of the present melanocyte activator can be obtained by extracting from the above-mentioned raw materials according to a conventional method. That is, the plant to be extracted is dried if necessary, and then immersed in the extraction solvent for a certain period of time, or brought into contact with the extraction solvent that has been heated to reflux, and then the extract is filtered and concentrated. Can be obtained. The extraction solvent to be used is not limited, but water, lower alcohols (especially methanol, ethanol, etc.), polyhydric alcohols (1,3-butylene glycol, etc.), acetone, ethyl acetate, etc. These mixtures are mentioned. Further, at the time of solvent extraction of plants belonging to the genus Cats such as Anemones, the extraction solvent is heated, for example, if the solvent is an ethanol-based solvent,
It is preferable to perform the solvent extraction at a temperature of the solvent of about 80 to 85 ° C., since an extract having strong cyclic AMP inducing ability is obtained.

[0015] One or more extracts of the genus Caterpillar thus prepared can be blended as an active ingredient of the present melanocyte activator. The blending amount of the extract of the genus Caterpillar in the melanocyte activator is
It can be freely selected depending on more specific applications, dosage forms, and the like, and is not particularly limited. If the compounding amount is dared to be specified, the amount of the agent in terms of dry mass is 0.1%.
0001 to 5% by mass, preferably 0.000 to 5% by mass.
0.01 to 1% by mass. If the amount is less than 0.00001% by mass in terms of dry mass, the amount of the agent may be too small to achieve the object of the present invention. Is allowed.

By administering (externally) the melanocyte activator, melanocytes in the vicinity of the administered part can be activated. Although the detailed mechanism has not been fully elucidated, melanocytes can be activated by at least a cyclic AMP inducing action and a tyrosinase activity promoting action.

That is, by administering the present melanocyte activator, cyclic AMP is induced in melanocytes.
Can induce melanin production, and by promoting tyrosinase activity, tyrosine can be used as a basis for melanin production.
-It is possible to accelerate the oxidation reaction to dihydroxyindole.

As described above, the administration of the present melanocyte activator activates the melanocytes in the vicinity of the administered portion, thereby exhibiting a gray hair preventing effect and a tanning effect.

The dosage and administration of the melanocyte activator is as follows:
There is no particular limitation, and it can be appropriately selected depending on the form of the agent, purpose, compounding amount of the active ingredient, and the like. For example,
When the melanocyte activator is a gray hair inhibitor,
In the case of adults, the extract of the extract of the genus Fenus ococcus, which is the active ingredient, can be applied so as to be 0.000001 to 100 mg, preferably 0.001 to 10 mg per day.

B. Embodiments of the present melanocyte activator The melanocyte activator is an agent that intends various effects by activating melanocytes. Typical examples thereof include an anti-whitening agent that activates melanocytes relating to hair to prevent gray hair, and a tanning agent that positively darkens skin for cosmetic reasons and the like.

When the melanocyte activator is a gray hair inhibitor, it is generally in the form of a scalp and hair preparation. When the melanocyte activator is a tanning agent, it is generally in the form of a skin external preparation.

Regardless of whether the melanocyte activator takes any of these forms, in addition to the inclusion of an extract of the genus Nematoda as an active ingredient, a preparation for scalp and hair such as a hair restorer and an external preparation for skin What is already used as a component of the agent, as long as the intended effect of the present invention is not impaired,
It can be blended as needed.

Specifically, diluents (water, ethanol, isopropyl alcohol, glycols, etc.), anionic surfactants (alkyl benzene sulfonate, polyoxyalkylene alkyl sulfate, alkyl sulfate, olefin sulfonic acid) Salt, alkyl phosphate, polyoxyalkylene alkyl ether phosphate,
Dialkyl sulfosuccinates and fatty acid salts), nonionic surfactants (polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyhydric alcohol fatty acid partial ester, polyoxyethylene polyhydric alcohol fatty acid partial ester, polyglycerin fatty acid ester) , Polyoxyethylene hydrogenated castor oil derivatives and fatty acid diethanolamides), cationic surfactants (tertiary aliphatic amine salts, alkyltrimethylammonium halides, dialkyldimethylammonium halides and alkyldimethylbenzylammonium halides), amphoteric surfactants Agents (amidobetaine type, imidazolinium betaine type and sulfobetaine type surfactants), higher alcohols (cetyl alcohol, stearyl alcohol, beheni Alcohol, etc.), oil (higher fatty acids, solid paraffin, liquid paraffin, silicone oil,
Polymeric silicone and its derivatives, squalane, petrolatum, ester oil, etc.), humectants (glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, etc.), thickeners (methyl cellulose, hydroxyethyl cellulose, carrageenan, Carboxymethylcellulose and cationized cellulose, etc.), solvents, usability improvers, preservatives, antioxidants, sequestering agents, UV inhibitors, powders (silica,
A resin powder such as a nylon powder or a polyethylene powder), a coloring agent, a fragrance and the like can be suitably used according to the intended dosage form to form a preparation.

In particular, when the present melanocyte activator is an anti-grey-hairing agent, if necessary, a hair-growing component may be blended to positively impart the hair-growing effect simultaneously with the anti-grey-hair effect. it can.

For example, plant extracts (such as assembly extracts and carrot extracts) which have a hair-growing effect, vitamins (vitamin B ₆ , vitamin E and its derivatives, vitamins such as biotin), pantothenic acid and its derivatives, Glycyrrhizic acid and its derivatives, nicotinic acid esters (benzyl nicotinate, etc.), amino acids (serine, methionine, arginine, etc.) cepharanthin, capronium chloride, minoxidil, nicorandil, acetylcholine derivatives, cyclosporins, female hormone agents (estradiol, etc.) ), Antibacterial agents (hinokitiol, hexachlorophen, benzalkonium chloride, cetylpyridinium chloride, undecylenic acid, trichlorocarbanilide and bithionol, etc.), cooling agents (menthol, etc.), salicylic acid, Lead and its derivatives, active substances (lactic acid and its alkyl esters, etc.), and the like organic acids (such as citric acid).

The form of the melanocyte activator is a liquid,
Emulsifiers, gelling agents, ointments, aerosols, mousses and the like may be used as long as they can be applied to the skin or scalp. In general, it may take a product form called emollient lotion, emollient cream, moisture gel, lotion, hair tonic, hair liquid, scalp treatment, hair cream, aerosol mousse, aerosol spray, hair gel, spray mousse, etc. it can.

[0027]

EXAMPLES Next, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples. The blending amount is mass% based on the blending target.

[Preparation Example] Preparation of extract (1 ) 200 g of whole plant (dry matter) of anemone (Wedelia chinensis MERR) was treated with 3 L of 100% ethanol [based on a special grade reagent of ethanol (hereinafter referred to as the same). )] For 10 days, and the solvent is distilled off from the extract.
10.5 g of extract was obtained (sample 1).

Preparation of extract (2) 240 g of whole plant (dry matter ) of anemone (Wedelia chinensis MERR) was soaked in 1.7 L of 80% ethanol and extracted at 80 to 85 ° C. for 2 hours. The solvent was distilled off from the extract to obtain 15.9 g of the extract (Sample 2).

Preparation of Extract (3 ) 150 g of whole plant (dried material) of anemone (Wedelia chinensis MERR) was added to 1.2 L of 8 L at room temperature for 10 days.
It is immersed in 0% ethanol, the solvent is distilled off from the extract,
6.9 g of extract was obtained (sample 3).

Preparation of Extract (4) 200 g of whole plant (dry matter ) of anemone (Wedelia chinensis MERR) was soaked in 1.5 L of 80% ethanol and extracted at 80 to 85 ° C. for 2 hours. After filtration, the concentration of ethanol was reduced to 50% by vacuum concentration, and
For one day and night, and after folding, the solvent was distilled off to obtain 12.4 g of an extract (sample 4).

Preparation of Extract (5 ) 150 g of whole plant (dried material) of anemone (Wedelia chinensis MERR) was added to 1.2 L of 8 L at room temperature for 10 days.
After immersion in 0% ethanol and filtration, the concentration of ethanol was reduced to 50% by concentration under reduced pressure, the mixture was allowed to stand at 5 ° C. for 24 hours, and after folding, the solvent was distilled off from the extract. 0.3 g of extract was obtained (sample 5).

[Test Example] Effect Confirmation Test Each of the above Samples 1 to 5 was dissolved in dimethyl sulfoxide (DMSO) so as to be 1% (dry mass), and the solution was diluted to adjust the concentration. The following test was performed using the obtained diluted solution.

Confirmation of effect by cell culture method In order to confirm the melanocyte activation effect by the cell culture method, mouse-derived B16 melanoma (cancerous melanocyte) cultured cells were used. Specifically, in a CO ₂ incubator (5% CO ₂ ) at 37 ° C.
Melanoma cells B16 were cultured in Eagle's MEM medium containing 3% fetal bovine serum (FBS).

The cultured melanoma cells were used for (1) a test for evaluating the ability to induce cyclic AMP. (2) (3) After 24 hours of culturing, each sample (1 to 5) was converted to a final concentration (concentration in terms of extracted dry matter) of 1 × 10 ⁻² to 1 × 10 ^−4.
% After addition to each culture system to give
The culture was continued for one day, and the sensation of melanin production was evaluated and the effect of promoting tyrosinase activity was measured.

(1) Evaluation of Cyclic AMP Inducibility 3 × 10 ⁴ cells per well in a 12-well plate
Each time, melanoma B16 cells were seeded and 24 hours later,
Each sample was 1 × 10 ^{-2 at the} final concentration (concentration in terms of extracted dry matter).
Or replace with a sample-containing medium containing 1 × 10 ^-4 mass%,
Incubated at 37 ° C. for 1 hour. The sample-supplemented medium was aspirated, 50 mM Tris HCl 4 mM EDTA solution was added, and the cells were scraped off with a scraper. The cell suspension was boiled at 100 ° C. for 5 minutes and centrifuged at 15000 rpm for 5 minutes. Using the obtained supernatant as a sample, cAMP EIA SYSTEM (Amasham LIFE S
CIENCE) kit, and the ability to induce cyclic AMP was evaluated according to the following criteria. Table 1 shows the results.

<Evaluation Criteria> A: cAMP inducing ability was more than 3 times that of control cells. ：: cAMP inducibility was 2-3 times that of control cells. △ to ｃ: cAMP inducibility was 1.
It was less than 5 to 2 times. Δ: cAMP inducing ability was 1 to 1.
It was less than 5 times. X: cAMP inducibility was less than 1 time of control cells.

Table 1 ──────────────────────────────────── Sample name (extract) Concentration ( %) Cyclic AMP inducibility ──────────────────────────────────── Sample 1 1 × 10 ^-2 ◎ Sample 1 1 × 10 ^-4 ○ to △ Sample 2 1 × 10 ^-2 ◎ Sample 2 1 × 10 ^-4 △ Sample 3 1 × 10 ^-2 △ Sample 3 1 × 10 ^-4 △ Sample 4 1 × 10 ^-2 ◎ Sample 4 1 × 10 ^-4 ◎ Sample 5 1 × 10 ^-2 ○ Sample 5 1 × 10 ^-4 △ ───────────────────────── ───────────

From these results, it was clarified that the ability to induce cyclic AMP in melanocytes was observed in all the extracts of antelope. In addition, it was revealed that the extracts of Sample 1, Sample 2 and Sample 4 were particularly excellent in the ability to induce cyclic AMP in melanocytes. In addition, especially, the anemone bloom is 80-8
Extraction with warming at 5 ° C. resulted in an extract with strong cyclic AMP-inducing ability, which proved to be suitable.

(2) Visibility determination of melanin content A diffusion plate was placed on the lid of the plate of the culture well of the cultured melanoma cells described above, the amount of melanin in the cells was observed with an inverted microscope, and the extract was added. It compared with the sample which did not do (reference). Table 2 shows the results.

<Judgment Criteria> +: Black (mostly melanin) ±: Slightly black (somewhat melanin) −: Control (melanin is hardly recognized)

(3) Measurement of Tyrosinase Activity The medium in the well was removed, and the well was washed twice with 100 μL of PBS. To each well, 45 μL of PBS containing 1% Triton-X (Rohm and Haas) was added. The plate was shaken for 1 minute to carefully break the cell membrane, and the absorbance at 475 nm was measured with a microplate reader. This was taken as the absorbance at 0 minutes. Then, quickly add 5 μL of 10
mM L-Dopa solution (a substrate for tyrosinase) was added to each well and transferred to a 37 ° C. incubator for 6 hours.
The reaction was performed for 0 minutes. After the reaction, the plate was shaken for 1 minute, and the absorbance (475 nm) at 60 minutes was measured. The increase in the absorbance difference of each extract-added sample with respect to the difference between the absorbance at 0 minutes of the sample without the plant extract (control) and at 60 minutes after the addition of dopa was determined as a percentage. Was determined as the tyrosinase activity promotion rate.
The higher the value, the higher the tyrosinase activity. The results are shown in Table 2.

Table 2 ──────────────────────────────────── Test Melanin production visual evaluation Tyrosinase Activity promotion rate (%) 濃度 Concentration (% by mass) 10 ^-4 10 ^-3 10 ^-2 10 ^-4 10 ^-3 10 ^-2 ─────────────────────────────────── ─ No addition---001 Control Kumquat extract---1 247 Sample 1 Kumquat extract--± 1 253 Sample 2 Kumquat extract----1 218 Sample 3 Kumquat extract--± 1 12 60 Sample 4 Anemone extract---1 2 23 Sample 5 ────────────────────────────────── ──

According to the results shown in Table 2, the melanin producing effect appealing to the sight was observed in Samples 2 and 4 and not observed in the other samples, but the promotion of tyrosinase activity was observed.
Found in all samples.

From these results, it was clarified that the tyrosinase activating effect was observed in the extract of Antrodia camphorata. In addition, it was revealed that the melanin-producing effect was remarkable in Samples 2 and 4 so as to appeal visually.

According to the test described above, the extract of antelope was added to
It was revealed that an activation effect at the cell level in melanocytes was observed. Further, it was clarified that a melanin-producing effect was visually recognized, and that a tanning effect was observed in the skin of the anemone extract.

Next, the action of the anemone extract on hair, that is, the effect of preventing gray hair, will be examined. (4) Human gray hair prevention effect test by cumulative application <Test method> As subjects, 15 males and females of 40 to 60 years old with gray hair, twice a day (morning and night) for four consecutive months for each sample (1 ~ 5 and a control sample without the extract of antelope extract)
The left and right scalps were separately used by the half head method, and the state of the application site was compared before and after the test, and the effect of preventing gray hair was examined.

More specifically, while applying a lotion containing each sample of the formulation described later every day, the ratio at which the application of this lotion prevents the generation of gray hair was determined by comparing the ratio of the head before and 4 months after the start of application. Top hair 1000
The number of white hairs per book was counted and evaluated by the evaluation criteria described later.

<Formulation of lotion> Ingredients% by mass Test substance 1.0 Polyvinylpyrrolidone / vinyl acetate copolymer 5.0 Preservatives Appropriate amount Perfume Appropriate amount Ethyl alcohol 30.0 Purified water 62.5 Silicone derivative 0. 5 glycerin 2.0 <Production method> A polymer, a preservative, and a fragrance were added to ethyl alcohol and uniformly dissolved. The aqueous phase (purified water, silicone derivative, glycerin) that had been dissolved in advance was added and dissolved to prepare a lotion.

The test substances were designated as Samples 1 to 5 (Anocula extract (dry matter)).
And 10. As a control, a product in which the test substance was replaced with purified water was used.

<Evaluation Criteria> ++ (Excellent effect): 50% or more of the subjects had less than 80% of the number of white hairs after application with respect to the number of white hairs before the application was started. + (Effective): The number of gray hairs after application to the number of gray hairs before the start of application was less than 90%, and 50% or more of the subjects. ± (Slightly effective): 50% or more of the subjects had less than 100% of the number of gray hairs after application with respect to the number of white hair before the application was started. -(Invalid): The number of gray hairs after application with respect to the number of gray hairs before the start of application was less than 50% for subjects having less than 100%. Table 3 shows the results of the test for preventing gray hair.

Table 3 被 験 Test substance Degree of prevention of gray hair ───────────── ───────────── Control product-Sample 6 + Sample 7 ++ Sample 8 ± Sample 9 ++ Sample 10 + ─────────────────── ──────── The results revealed that the present melanocyte activator had an effect of preventing gray hair. In particular, the agents (Samples 7 and 9) in which Samples 2 and 4 were blended exhibited a suitable gray hair preventing effect.

Hereinafter, examples of the formulation of the present melanocyte activator will be described. In addition, among these preparation examples, preparation examples 1 to 13
Showed a gray hair preventing effect, and Formulation Examples 14 to 17 showed a tanning effect.

[Preparation Example 1] Hair tonic blending component blending amount (% by mass) (1) Hydrogenated castor oil EO (40 mol) adduct 2.0 (2) Kumanoki extract (sample 4 dried product) 0.1 ( 3) 95% ethanol 70.0 (4) Appropriate amount of fragrance (5) Purified water residue <Production method> (1) (2) (4) was added to (3), and dissolved by stirring. In addition, I got a hair tonic.

[Preparation Example 2] Hair tonic blending component blending amount (% by mass) (1) glycerin 2.0 (2) L-menthol 0.1 (3) Kumanoki extract (sample 3 dried product) 0.5 ( 4) 95% ethanol 60.0 (5) Appropriate amount of fragrance (6) Purified water residue <Production method> (1), (2), (3) and (5) were added to (4), and dissolved by stirring. (6) was added to obtain a hair tonic.

[Preparation Example 3] Hair tonic blending component blending amount (% by mass) (1) glycerin 2.0 (2) L-menthol 0.1 (3) decyltetradecyldimethylamine oxide 0.8 (4) Kumanogiku Extract (sample 4 dried product) 0.001 (5) 95% ethanol 70.0 (6) Appropriate amount of perfume (7) Purified water residue <Production method> (5), (1) (2) (3) (4) After adding (6) and stirring and dissolving, (7) was added to obtain a hair tonic.

[Formulation Example 4] Compounding amount of hair tonic compound (% by mass) (1) Glycerin 2.0 (2) L-menthol 0.1 (3) Kuddin extraction liquid 0.5 (4) Kumanogus extract ( (Sample 2 dried product) 0.01 (5) 95% ethanol 70.0 (6) Perfume appropriate amount (7) Purified water residue <Production method> (5), (1) (2) (3) (4) After adding (6) and dissolving with stirring, (7) was added to obtain a hair tonic.

Formulation Example 5 Hair Tonic Compounding Component Blending Amount (% by mass) (1) Glycerin 2.0% by mass (2) L-Menthol 0.1 (3) Panax ginseng extract 1.0 (4) Kumanogus extract (Sample 2 dried product) 0.01 (5) 95% ethanol 65.0 (6) Appropriate amount of perfume (7) Purified water residue <Production method> (5), (1) (2) (3) (4) ) (6) was added and dissolved by stirring, and then (7) was added to obtain a hair tonic.

[Formulation Example 6] Hair tonic compounding ingredients (% by mass) (1) Glycerin 2.0 (2) L-menthol 0.1 (3) Decyltetradecyldimethylamine oxide 0.8 (4) Kuzin Extracted liquid 0.5 (5) Quacharate extract 0.1 (6) Anemone extract (dried sample 4) 0.0001 (7) 95% ethanol 60.0 (8) Perfume qs (9) Purified water Residual <Production method> (7), (1) (2) (3) (4) (5)
After adding (6) and stirring and dissolving, (9) was added to obtain a hair tonic.

Formulation Example 7 Hair Tonic Ingredient Compounding Amount (% by Mass) (1) Polyethylene Glycol 200 2.0 (2) L-Menthol 0.2 (3) Kumanoki Extract (Sample 1 Dried Product) 0 (4) 95% ethanol 50.0 (5) Perfume appropriate amount (6) Purified water residue <Production method> (1) (2) (3) (5) was added to (4),
After stirring and dissolving, (6) was added to obtain a hair tonic.

Formulation Example 8 Hair Liquid Compounding Component Blending Amount (% by mass) (1) Polyoxypropyl butyl ether (40PO) 15.0 (2) Polyoxypropyl butyl ether phosphoric acid (40PO) 15.0 (3) Kumanogiku Extract (sample 4 dried product) 2.0 (4) 1,3-butylene glycol 5.0 (5) 95% ethanol 50.0 (6) Flavor appropriate amount (7) Dye, edetic acid appropriate amount (8) Purified water residue <Production method> (5), (1) (2) (3) (4) (6)
After adding (7) and dissolving with stirring, (8) was added to obtain a hair liquid.

[Formulation Example 9] Scalp treatment compounding components (% by mass) (1) 1,3-propylene glycol 0.5 (2) pentaerythritol tetra-2-ethylhexanate 1.2 (3) 95% Ethanol 60.0 (4) Antelope extract (dried sample 2) 0.05 (5) Assembly extract 1.0 (6) Appropriate amount of flavor (7) DME / LPG (95/5) Residue <Production method> ( To (3), (1), (2), (4), (5), and (6) were added and dissolved by stirring.
(7) was filled to obtain a spray type scalp treatment.

[Formulation Example 10] Amount of hair cream components (% by mass) Phase A (1) Liquid paraffin 5.0 (2) Cetostearyl alcohol 5.5 (3) Vaseline 5.5 (4) Glyceryl monostea Rate 3.0 (5) EO (20 mole addition) -2-octyldodecyl ether 3.0 (6) Vitamin E acetate 0.05 (7) Propyl paraben 0.3 (8) Fragrance 0.05 B phase (9 ) Anemone extract (sample 5 dried product) 4.0 (10) glycerin 7.0 (11) dipropylene glycol 20.0 (12) polyoxyethylene glycol 4000 5.0 (13) sodium hexametaphosphate 0.005 ( 14) Purified water Residue <Production method> Phase A is dissolved and mixed by heating, and the hot-melt mixture of Phase B is added thereto, and emulsified by a homomixer. To obtain a stream.

Formulation Example 11 Hair gel compounding component compounding amount (% by mass) (1) Carboxyvinyl polymer 0.7 (2) Polyvinylpyrrolidone 2.0 (3) Glycerin 4.0 (4) Kumanogus extract (sample 3) (Dried product) 2.0 (5) Sodium hydroxide qs (6) Ethyl alcohol 20.0 (7) Polyoxyethylene octyl dodecyl ether qs (8) Flavor, chelating agent (EDTA) qs (9) Purified water Residue <Production method> Disperse the carboxyvinyl polymer in glycerin and some purified water. Other components were dissolved in the remaining purified water and added with stirring to obtain a hair gel.

Formulation Example 12 Water grease compounding component compounding amount (% by mass) (1) Carboxyvinyl polymer 0.5 (2) Glycerin 50.0 (3) Sodium hydroxide proper amount (4) Ethyl alcohol 10.0 (5) Anemone extract (sample 4 dried product) 3.0 (6) Polyoxyethylene octyl dodecyl ether qs (7) Flavor, chelating agent (EDTA) qs (8) Purified water residue <Preparation method> Carboxyvinyl The polymer was dispersed in glycerin and part of purified water, and the other components were dissolved in the remaining part of purified water and added with stirring to obtain a water grease.

Formulation Example 13 Hair Spray Component Ingredients (% by mass) Stock solution formulation (1) Acrylic resin alkanolamine solution (50%) 7.0 (2) Cetyl alcohol 0.1 (3) Silicone oil (methyl) (Phenylpolysiloxane) 0.3 (4) Ethyl alcohol 92.5 (5) Kumanoki extract (sample 5 dried product) 0.01 (6) Perfume appropriate amount filling formulation (7) Stock solution 50.0 (8) Dimethyl ether 45 0.0 (9) LPG 5.0 <Production method> (1) (2) (3) was uniformly emulsified with a homomixer. This was added to a solution of other components to prepare a stock solution. For filling, a can was filled with a stock solution, a valve was attached, and then gas was filled to obtain a hair spray.

[Formulation Example 14] Blending amount of toner component (% by mass) (1) 1,3-butylene glycol 6.0 (2) glycerin 4.0 (3) oleyl alcohol 0.1 (4) POE ( 20) Sorbitan monolaurate 0.5 (5) POE (15) Lauryl alcohol ether 0.5 (6) Kumanoki extract (sample 4 dried) 1.0 (7) Ethanol 10.0 (8) Fragrance, pigment , Preservative, anti-fading agent and buffer solution (9) Purified water residue <Production method> Dissolve the buffer and anti-fading agent of (1) and (2) and (8) in (9) at room temperature. The aqueous phase was used.
In (7), the fragrance of (8), (3), (4), (5) and (6) are dissolved and mixed and solubilized in the aqueous phase. afterwards,
Toning was performed using the coloring agent (8) to obtain a lotion.

[0068] [Formulation Example 15] Emollient Lotion Ingredients Amount (wt%) (1) Stearic acid 2.0 (2) Cetyl alcohol 1.5 (3) Vaseline 4.0 (4) Squalane 5.0 (5 ) Kumanoki extract (Sample 3 dried product) 5.0 (6) Glycerol tri-2-ethylhexanoate 2.0 (7) Sorbitan monooleate 2.0 (8) Dipropylene glycol 5.0 (9) ) PEG 1500 3.0 (10) Triethanolamine 1.0 (11) Perfume and preservatives Each appropriate amount (12) Purified water residue <Production method> Other ingredients are stirred and heated and dissolved in (12), emollient lotion I got

[Formulation Example 16] Amount of emollient cream compounding component (% by mass) (1) Cetyl alcohol 5.0 (2) Stearic acid 3.0 (3) Vaseline 5.0 (4) Squalane 10.0 (5 ) Glycerol tri-2-ethylhexanoate 7.0 (6) Kumquat extract (sample 1 dried product) 0.5 (7) Dipropylene glycol 5.0 (8) Glycerin 5.0 (9) Propylene glycol monostearin Acid ester 8.0 (10) POE (20) Cetyl alcohol ether 3.0 (11) Triethanolamine 1.0 (12) Perfume, preservative and antioxidant Appropriate amount (13) Purified water residue <Production method> (13), (6) (7) (8) (10) (1
1) (12) was added, and the mixture was dissolved by heating and kept at 70 ° C. (aqueous phase). The oil phase is added to the aqueous phase, pre-emulsified, homogenized uniformly with a homomixer, and then emulsified.
After cooling to 0 ° C., an emollient cream was obtained.

[Formulation Example 17] Moisture gel blending component blending amount (% by mass) (1) dipropylene glycol 7.0 (2) PEG 1500 8.0 (3) Kumanoki extract (sample 3 dried product) 0.1 ( 4) Carboxyvinyl polymer 0.4 (5) Methyl cellulose 0.2 (6) POE (15) Oleyl alcohol ether 1.0 (7) Potassium hydroxide 0.1 (8) Fragrance, coloring matter, preservative, anti-fading agent (9) Purified water residue <Preparation method> A carboxyvinyl polymer is dispersed in glycerin and a part of purified water, and this dispersion is dissolved in other components in the remaining purified water. The mixture was added with stirring to obtain a moisture gel.

[0071]

According to the present invention, there is provided an agent having an excellent melanocyte activating action which can be used as a graying inhibitor or a tanning agent.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Eiji Ifuku 2-2-1 Hayabuchi, Tsuzuki-ku, Yokohama-shi, Kanagawa Prefecture Inside Shiseido Research Center (Shin-Yokohama) (72) Inventor Koji Kobayashi Yokohama-shi, Kanagawa 2-2-1 Hayabuchi, Tsuzuki-ku Shiseido Research Center (Shin-Yokohama) Co., Ltd. (72) Inventor Masahiro Tajima 2-2-1 Hayabuchi, Tsuzuki-ku, Yokohama-shi, Kanagawa Shiseido Research Center (Shin-Yokohama) F term (reference) 4C083 AA111 AA112 AB032 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC402 AC422 AC432 AC442 AC482 AC532 AC542 AC562 AC902 AD042 AD072 AD092 AD112 AD152 AD262 AD532 AD662 CC04 CC05 CC33 DD08 DD22 DD23 DD27 DD31 DD41 DC42 AC24 AC01 AC05 CA03 MA63 NA14 ZA92 ZC02 ZC41

Claims (4)

[Claims] [Claim 1] Asteraceae (Compositae)
a melanocyte activator comprising an extract of a plant belonging to lia) as an active ingredient. 2. The melanocyte activator is an anti-whitening agent.
The melanocyte activator according to claim 1. 3. The genus Compositae (Wede)
lia) is a plant belonging to the anemone tree (scientific name: Wedelia chin)
The melanocyte activator according to claim 1 or 2 which is an ensis MERR). 4. A genus of the family Asteraceae (Compositae)
lia) is 0.0001 of the agent in terms of dry mass.
The melanocyte activator according to any one of claims 1 to 3, wherein the melanocyte activator is contained in an amount of from 1 to 1% by mass.