JPH10503846A - 電極およびその分析における使用 - Google Patents
電極およびその分析における使用Info
- Publication number
- JPH10503846A JPH10503846A JP8506319A JP50631996A JPH10503846A JP H10503846 A JPH10503846 A JP H10503846A JP 8506319 A JP8506319 A JP 8506319A JP 50631996 A JP50631996 A JP 50631996A JP H10503846 A JPH10503846 A JP H10503846A
- Authority
- JP
- Japan
- Prior art keywords
- polymer
- electrode
- enzyme
- concentration
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 溶液中の物質の濃度を示すための方法であって、伝導率の異なる酸化状態 および還元状態のいずれか一方の状態にあるポリマーで被覆された第1の電極構 造と、第2の別の対電極との間に溶液中で交流電圧をかけ、ポリマー被覆の伝導 率の変化を測定し、該測定の結果はポリマーの状態を示し、したがって溶液中の 前記物質の濃度を示すことを特徴とする方法。 2. 第1の測定は溶液中の物質の非存在下で実施され、前記溶液中における物 質の干渉についてのバックグラウンド読み取り値を提供することを特徴とする請 求の範囲第1項に記載の方法。 3. 酵素はポリマー被膜に固定され、前記方法は溶液中における前記酵素の基 質の濃度を示すためのものであることを特徴とする請求の範囲第1項に記載の方 法。 4. 前記第1の電極は、基質とポリマー薄膜との間で電荷を移動させるために ポリマーまたは酵素被膜に関連したメディエータを備えることを特徴とする請求 の範囲第3項に記載の方法。 5. 第1の測定は前記酵素の非存在下で実施され、前記溶液中における物質の 干渉についてのバックグラウンド読み取り値を提供することを特徴とする請求の 範囲第3項または第4項に記載の方法。 6. 酵素の基質はポリマー被膜に固定され、前記方法は溶液中における酵素の 濃度を示すためのものであることを特徴とする請求の範囲第1項または第2項に 記載の方法。 7. ポリマーはポリ(アニリン)を有することを特徴とする請求の範囲第1項 乃至第 6項のいずれか1つに記載の方法。 8. ポリマー薄膜の厚さは0.1〜1μmの範囲にあることを特徴とする請求の範 囲第1項乃至第7項のいずれか1つに記載の方法。 9. 第1の電極構造は、表面に離散的ポリマー被膜が使用された複数の電極を 備え、各電極に別々に交流電圧が印可され、各ポリマー被膜も伝導率の状態を測 定することによって、溶液中の複数の物質の濃度を測定することを特徴とする請 求の範囲第1項乃至第8項のいずれか1つに記載の方法。 10.請求の範囲第1項乃至第9項のいずれか1つに記載の方法において使用さ れる回路の単極を形成する電極構造であって、ポリマー薄膜が被覆された電極を 備え、ポリマーは伝導率の異なる酸化状態および還元状態のいずれか一方の状態 にあることを特徴とする電極構造。 11.ポリマーが被覆される露出面を有する細長い電極を備えることを特徴とす る請求の範囲第10項に記載の電極構造。 12.ポリマー薄膜は、酵素が固定される組成物によって被覆されていることを 特徴とする請求の範囲第10項または第11項に記載の電極構造。 13.前記電極は、基質に印刷された絶縁誘電層と層間接続された1本以上の炭 素電極を有する炭素マイクロバンド電極を有し、ポリマーは前記マイクロバンド 電極の炭素の露出端に被覆されていることを特徴とする請求の範囲第10項乃至 第12項のいずれか1つに記載の電極構造。 14.前記ポリマー薄膜の厚さは0.1〜1μmの範囲にあることを特徴とする請求 の範囲第10項乃至第13項のいずれか1つに記載の電極構造。 15.離散的ポリマー薄膜で被覆された複数の電極を有することを特徴とする請 求の範囲第10項乃至第14項のいずれか1つに記載の電極構造。 16.酵素は離散的ポリマー薄膜の1つにオーバーコートされていることを特徴 とする請求の範囲第15項に記載の電極構造。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9415499.4 | 1994-08-01 | ||
GB9415499A GB9415499D0 (en) | 1994-08-01 | 1994-08-01 | Electrodes and their use in analysis |
PCT/GB1995/001818 WO1996004398A1 (en) | 1994-08-01 | 1995-08-01 | Electrodes and their use in analysis |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10503846A true JPH10503846A (ja) | 1998-04-07 |
Family
ID=10759219
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8506319A Ceased JPH10503846A (ja) | 1994-08-01 | 1995-08-01 | 電極およびその分析における使用 |
Country Status (9)
Country | Link |
---|---|
US (1) | US5849174A (ja) |
EP (1) | EP0775214B1 (ja) |
JP (1) | JPH10503846A (ja) |
AT (1) | ATE251673T1 (ja) |
AU (1) | AU3182395A (ja) |
CA (1) | CA2196380A1 (ja) |
DE (1) | DE69531897D1 (ja) |
GB (1) | GB9415499D0 (ja) |
WO (1) | WO1996004398A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008504881A (ja) * | 2004-07-01 | 2008-02-21 | ヴィヴォメディカル, インコーポレイテッド | 非侵襲性グルコース測定 |
JP2012023224A (ja) * | 2010-07-15 | 2012-02-02 | National Institute Of Advanced Industrial & Technology | 多孔質性電極を用いた電気化学デバイス |
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-
1994
- 1994-08-01 GB GB9415499A patent/GB9415499D0/en active Pending
-
1995
- 1995-08-01 DE DE69531897T patent/DE69531897D1/de not_active Expired - Lifetime
- 1995-08-01 AU AU31823/95A patent/AU3182395A/en not_active Abandoned
- 1995-08-01 JP JP8506319A patent/JPH10503846A/ja not_active Ceased
- 1995-08-01 AT AT95927815T patent/ATE251673T1/de not_active IP Right Cessation
- 1995-08-01 EP EP95927815A patent/EP0775214B1/en not_active Expired - Lifetime
- 1995-08-01 CA CA002196380A patent/CA2196380A1/en not_active Abandoned
- 1995-08-01 WO PCT/GB1995/001818 patent/WO1996004398A1/en active IP Right Grant
- 1995-08-01 US US08/776,716 patent/US5849174A/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008504881A (ja) * | 2004-07-01 | 2008-02-21 | ヴィヴォメディカル, インコーポレイテッド | 非侵襲性グルコース測定 |
JP2012023224A (ja) * | 2010-07-15 | 2012-02-02 | National Institute Of Advanced Industrial & Technology | 多孔質性電極を用いた電気化学デバイス |
Also Published As
Publication number | Publication date |
---|---|
US5849174A (en) | 1998-12-15 |
CA2196380A1 (en) | 1996-02-15 |
EP0775214B1 (en) | 2003-10-08 |
GB9415499D0 (en) | 1994-09-21 |
EP0775214A1 (en) | 1997-05-28 |
ATE251673T1 (de) | 2003-10-15 |
WO1996004398A1 (en) | 1996-02-15 |
AU3182395A (en) | 1996-03-04 |
DE69531897D1 (de) | 2003-11-13 |
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