JPH10323168A - Anticancer material comprising extract of fuscoporia obliqua cultured in sawdust - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a medicine suitable for oral administration or food containing an active component obtained by preparing a Fuscoporia obliqua mycelium having an anticancer action equivalent to a natural product by an artificial culture using a sawdust medium of birches and extracting from the mycelium. SOLUTION: This anticancer material comprises an extract of Fuscoporia obliqua. Sawdust of Betula platyphylla var. japonica or Betula ermani are mixed with calcium hydroxide and rice bran, and shaken, and then mixed with water so that the water content is made to be about 70% of whole of the mixture. When a central temperature of the sawdust medium lowers to below 20 deg.C, pure mycelia of the Fuscoporia obliqua are planted and cultured. Grown mycelia are subjected to a boiling extraction treatment or a solvent extraction treatment and extracted active components are made into a medicine suitable for oral administration or food.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a medicament or health comprising an extract of birch mycelium cultured on a medium containing, as a main essential medium, a mycelium of cultured birch, especially birch sawdust (wood chips). The present invention relates to an anticancer substance suitable for oral ingestion as a food.

[0002]

[Background of the Invention] Birch mushroom [scientific name: Fuscoporia obli
qua (Fr.) Aoshima] is naturally found on trees in the mountains and grows into a coal-like black mass, but originally inhibits tree growth as a tree cancer. It is a mushroom. The present inventor has conducted various studies on this mushroom, which has generally been treated as a bad person as a disease of a tree, as to whether there is no way to use it effectively or whether there is a corporate use method with food suitability. As a result, they have found that the mycelium of B. oleracea has an excellent anticancer effect, and that the mycelium has food suitability to give food and drink an anticancer effect.

Birch mushrooms grown and adhered to birch species in nature have been found to have a cancer-suppressing effect by themselves. Due to poor processing suitability when processing into food, and because it takes a lot of labor and long time to collect a certain amount of the same thing, using natural products as a base is not suitable for commercialization in the first place Met.

[0004] For example, when birch mushroom powder is used as a substitute for tea, the natural product is hard with the appearance of a piece of coal, it takes a long time to extract components, and it takes a long time to pulverize it even in a food. However, there was a problem that the flavor was not good.

[0005]

Therefore, the present invention is based on the finding that birch mushrooms have an anticancer effect, and it has been determined that they contain various components equivalent to those of naturally occurring birch mushrooms by a specific culture method. Cultured in large quantities and constantly throughout the year, collected, and the active ingredients are extracted to produce anticancer substances suitable for oral ingestion, that is, ingestible cancer suppressants and foods (functional foods). The purpose is to:

[0006]

SUMMARY OF THE INVENTION In order to achieve the above object, the present invention relates to a special mushroom called birch mushroom, which is cultured using a specific sawdust culture method separately established by the present inventors, which is equivalent to a naturally occurring birch mushroom. The birch mycelium containing various components, a liquid obtained by boiled extraction or solvent extraction, or a powder obtained by drying it as an active ingredient, with or without adding additives as appropriate And a liquid antimicrobial agent that can be taken, and the liquid active ingredient or the cultured mycelium is dried under appropriate (non-discoloring) temperature conditions to obtain a powder, which is then mixed with various foods to have an anticancer function. Food.

[0007] The method of cultivating the mycelium of B. aeruginosa which is specifically required to obtain the anticancer substance (anticancer agent / anticancer functional food) of the present invention forms the basis of the present invention. .

Preparation of culture medium : In order to prepare a culture medium uniquely suited for cultivation of birch mushrooms, four pieces of birch (birch or birch birch) wood chips, or sawdust, are placed in a mini-container having a capacity of 432 cm ³ , and 100 cc of slaked lime are added thereto. ,
Further, half a cup of rice bran is added in the same mini-container and stirred, and water is added so that the water content is about 70% of the whole mixture. At this time, another commercially available hyphal growth promoter safe to the animal body may be added. (For example, wheat bran)

The above mixture is placed in a heat-resistant bag or container and heated so as to be sterile. Usually put in a steam steamer (retort container may be used) at 100 ° C for 8 hours.
This steaming is for sterilization and does not elute and remove various woody components originally contained in birch or birch birch, particularly compounds or polysaccharides having a phenolic hydroxyl group or an alkoxy group. The sterilized medium as described above is used as a sawdust medium, which is cooled prior to inoculation. During cooling,
Care should be taken not to enter any germs as the outside air is sucked into the bag or container.

Inoculation : When the center temperature of the sawdust medium drops to the growth temperature of the birch mycelium, preferably 20 ° C. or less,
Inoculate a pure hypha of birch. Create a room where the surroundings can maintain a sterile environment, and cover the bag or container containing the medium. Instruments such as spoons for inoculation are baked and sterilized to prevent germs. Worker's hands should be disinfected with alcohol or the like, put on a mask, put on a white coat, etc., so as to prevent falling and mixing of various bacteria. At the time of inoculation, a hole having a diameter of about 2 to 3 cm may be opened downward (in the case of a bag, so as not to break the bag) in the center of the medium floor.

If the medium is about 15 cm high and one side of the medium is about 10 cm square, a hole having a diameter of about 2 to 3 cm is formed in the center at about 10 cm.
It is preferred that the mycelium be grown at a depth of about 1 to allow the mycelium to grow on the sawdust medium more quickly. At the time of inoculation, it is convenient to disperse the mycelium at the hole so that the mycelium is scattered on the upper surface of the medium. Even in the case of a large medium (assuming a depth of 15 cm), a hole having a diameter of 2 to 3 cm and a depth of about 10 cm is preferably formed in one 10 cm square.

As a comparative example, an experiment was conducted using sawdust of trees other than birch. In other words, using a medium made of sawdust such as deciduous trees (eg, beech and oak) and fir pine, the same method as that performed on the birch medium was tested.
In the medium of sawdust other than these birch species, the hypha of birch was not able to grow. From this, it is recognized that it is necessary to specifically use birch trees, especially birch and birch sawdust (wood chips) as the main essential material of the culture medium in order to artificially grow the mycelium of the birch. .

Growth : The medium inoculated as described above is at 13 ° C.
It is good to keep the temperature condition from to 33 ° C. Mycelium does not grow well, even if it is too cold or hot. As a guideline for cultivation on a company scale, it is better to control the temperature with about 20 ° C as a guide,
An indoor temperature of about 20 ° C. is preferable because germs and mold are less scattered, and the danger that the culture medium is violated by mold during culture is reduced.

The culture placed in the container is placed on a shelf with good air circulation, and is irrigated once or twice a day. The room humidity is maintained so as to maintain 80% or more. It is advisable to spray water twice a day so that the floor does not dry out. Although it depends on the size of the sawdust medium, the hypha usually grows white in the medium in about four months and fills the entire surface of the medium.

Collection : The grown mycelium is appropriately collected, processed according to a specific purpose (for example, extraction of an active ingredient, concentration or dilution of an extract, dry powdering, baking, etc.) and then as a drug. Or it can be used as a functional food. Mycelium cultured in white birch waste medium is easier to pulverize by freeze-drying and the like than mycelium cultured in a liquid medium, and the powder can be kept in a dry state for a long time and has good storage stability. There are advantages. The use of the birch sawdust medium as described above suggests that the resulting birch mycelium contains a potent active ingredient comparable to that of natural products, as described in the mouse experiments described below.

The effect of the birch mycelium obtained by the above-described method for cultivating birch mushrooms on the specific birch mushrooms, in particular, on the anticancer properties, in particular, the ability to suppress cancer by oral ingestion, was confirmed by mouse experiments.

Test purpose : Fucoporia obl
In vivo of extract from iqua (Fr.) Aoshima
The antitumor effect is evaluated in a system using a mouse experimental tumor.

[0018]Test method1. Experimental animal BALB / c mouse (male) 5-6 weeks old 2. Experimental tumor Meth A fibrosarcoma (methylcholanthrene-induced glandular fibrosis
Tumor) 3. Administration method of birch mushroom extract 3-1 Preparation of test solution: specimen (mycelium collected from culture medium)
3 g of distilled water (300 g)
At 100 ° C for 20 minutes. Filter the extract
After that, it was stored at -20 ° C until use. In addition, engraftment of hypha
A similar extract from sawdust without grease was used as a control.
Was. 3-2 Administration route: oral ingestion 4. Therapeutic experiment Tumor cells (in the case of Meth A 2 × 1) in BALB / c mice
0⁶) Was implanted subcutaneously in the right flank, 9 days after Meth A transplantation
More mice were given birch extract (treatment group) or
The light extract (control group) was orally freely ingested. Twice a week, each
The tumor volume of the mouse was measured, and the control group and the treatment group were compared. 5.Measurement of tumor volume Measure the major axis and minor axis of the tumor mass with calipers and use the following formula
Calculated. Tumor volume (mm^Two) = 1/2 × (major axis) × (minor axis)^Two  6. Statistical significance test Mann-Whitney U-test (P <0.05)

Comparative Results : Treatment Experiment for Meth A FIG. 1 shows the tumor growth curves of the control group and the treatment group. Tumor volume of treatment group was statistically significant from day 16 after tumor implantation
(P <0.05). Note: The change in tumor volume in the group that had free access to tap water instead of the extract was similar to the control group in this experiment.

Conclusion : The anti-tumor effect of the birch mushroom extract of the present invention cultured in a specific medium was evaluated by ingesting a tumor-bearing mouse freely orally. Tren-induced fibrosarcoma).

Thus, it is recognized that the extract of B. amanita mycelium cultured by the above-mentioned specific culture method has a clear anticancer effect as shown in FIG. 1 by the method of oral free intake. The birch mycelium obtained in this particular birch sawdust medium is extremely important and significant in that mouse experiments were performed by oral, not gavage, administration of the mycelium extract. It has properties (taste, aroma, etc.) suitable for natural ingestion from the mouth as medicines and foods, and shows that medicinal ingredients act strongly in vivo. (Drugs taken freely by mouth are usually diminished in efficacy due to stomach acid and physiological reactions in the body. If you do not have a strong medicinal component, it will often be ineffective even if you take it by mouth.) The nature that it can be taken naturally means that the cultured mycelium used in the present invention can be freely taken orally as an anticancer food or drug (can be taken).
It has a wide range of applications that can be processed as a substance.

According to the present invention, the mycelium of B. oleracea obtained by the specific culturing method as described above is collected, and the active ingredient in liquid form is obtained by extraction by boiling or extraction with a solvent. The actual example of boiled-out extraction is the test method described above.
Basically the same as described in section 3 of the method , except that the type of water used (whether or not distilled water), the ratio of water to mycelium, the extraction temperature, and the extraction time are particularly limited. is not. The storage temperature of the extract is likewise not limiting. Birch mushroom extract, as it is, diluted or undiluted,
Can be taken orally. On the contrary, when the extract is boiled and extracted with a large amount of water, the extract can be concentrated. These diluted, undiluted or concentrated liquids are the anticancer substances of the present invention. Alternatively, the extract may be powdered by freeze drying or heat drying, and the powder may be taken together with or dissolved in water. In this case, the powder itself or its aqueous solution is the anticancer substance of the present invention.

As an example of the use as an anticancer agent of the present invention, for humans, for example, for stomach cancer, the body weight is 60 kg.
It is recognized that there is an anti-cancer effect when decoction is taken with the same amount of water, with about 6 kg (20 g / day) per month as a guideline for a standard person. Drinking naturally as an aqueous solution is very significant for clothing. The cultured mycelium of the birch mushroom used in the present invention, compared to a conventionally known liquid culture,
For example, powderization by freeze-drying method is easy,
Since the powder can be kept dry for a long period of time, it is excellent in commercialization.

There are various applications of the anticancer substance of the present invention as a food, but in order to make it a food contaminant, first, a sawdust culture of birch is dried by heating or drying under vacuum at a low temperature. At this time, the culture of the birch mushroom may be appropriately heat-sterilized at 100 ° C. and then dried. If the heat drying is not performed at a temperature that does not cause discoloration, the flavor is not good and food suitability is impaired. Care must be taken as drying at high temperatures will result in a burning odor and reduced active ingredients.

When the dried mycelium of the above-described cultured birch is obtained, for example, packed in a tea bag, mushroom tea that can be easily commercialized is obtained. Since this mushroom tea can be taken on a daily basis, it is also convenient for healthy living. Since the dried culture is in the form of particles, it is easy to carry out various post-processing, and if necessary, further pulverized or extracted to obtain various foods or drinks (in this specification, drinks and drinks). (Collectively referred to as "foods").

Examples of foods which can be mixed with the cultured birch extract of the present invention include gum, caramel, candy, chocolate, jam, noodles, miso, drinks, pet food, meat dumplings, and the flow of sick people. Food, seasonings (miso, soy sauce, soup ingredients, etc.), bread, donut,
Egg fried, sausage, ham, fish paste, seaweed tsukudani, dango, kibi-dango, wine, beer, shochu, whiskey, soft drink, fruit or vegetable juice, green tea, bancha, matcha, oolong tea, and the like. By mixing the above-mentioned foods with the powder or extract of the cultured dried birch mushroom of the present invention, it does not cause off-flavors or the like, is easy to ingest, and is a convenient functional food having an anticancer effect. It can be.

[0027]

As described above, the present invention paves the way for utilizing pathogens of trees, which have not been considered in the past, as anti-cancer substances for animals. Based on a simple artificial propagation method,
Anticancer substances (drugs) suitable for oral ingestion that are not obtained by conventional liquid culture, and are collected and processed in mass production of mycelium containing active ingredients that are as powerful as those of natural products. Food), it can have a significant effect on the maintenance and promotion of human and other animal health.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows the results of a tumor treatment experiment using a mouse to which a birch mushroom culture of the present invention was orally freely ingested (black circles).
Is a graph showing a comparison with the control example (open circles), in which the vertical axis represents tumor volume (mm ² ) and the horizontal axis represents days after tumor implantation.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI C12R 1: 645)

Claims (1)

[Claims] Claims: 1. A natural birch tree birch tree selected from the group consisting of birch and birch birch is used as a main essential medium material and grown and grown on a medium prepared so as not to impair the components originally contained therein. Anticancer properties suitable for oral or oral ingestion as a drug or food, with liquid or dry powder obtained by boiling or solvent-extracting the mycelium of birch mushrooms containing various components equivalent to those produced in Japan. material.