JPH0840824A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic for improving skin roughness. CONSTITUTION:The characteristic of the cosmetic comprises containing secretory immunoglobulin A and lactoferrin. The secretory immunoglobulin A is contained in a mucosal secretory solution and plays an important role on biophylaxis in a local immune mechanism. The lactoferrin is a glucoprotein and takes iron ions from bacteria to exhibit an antibacterial effect. The preparation forms of the cosmetic include creams, lotions, emulsions, foundations, lip sticks, eye shadows, and hair liquids.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a cosmetic containing secretory immunoglobulin A and lactoferrin.

[0002]

2. Description of the Related Art Infectious dermatitis such as pyoderma pyoderma and pyoderma pyoderma is caused by Pseudomonas aeruginosa such as Staphylococcus aureus and Streptococcus. Further, it is known that one of the causes of atopic dermatitis is Staphylococcus aureus that grows on the skin. Therefore, external preparations for skin to which antibiotics, bactericides and antibacterial agents have been added have been conventionally used for their treatment. In addition, there has been no conventional cosmetic containing a substance that suppresses the growth of only Staphylococcus aureus.

[0003]

However, some of the bactericides and antiseptics have a problem in safety for human body due to skin irritation, and the external preparations containing these agents are suppuratives. Not only fungi and general bacteria but also a bactericidal effect against skin resident bacteria such as Staphylococcus epidermidis, it may sometimes break the delicate balance of skin resident bacteria by daily use, sometimes from the viewpoint of so-called ecology It has been pointed out that it causes an unfavorable situation. For example, Staphylococcus epidermidis has a skin-purifying action of degrading triglyceride in sebum by the action of its lipase and keeping the pH on the skin acidic. Therefore, it is possible to selectively suppress Pseudomonas aeruginosa such as Staphylococcus aureus and Streptococcus without affecting the Staphylococcus epidermidis without using such antibiotics, bactericides or antibacterial agents, and affect the living body. There has been a search for highly safe substances without giving.

It has been conventionally known that secretory immunoglobulin A and lactoferrin are contained in mucosal secretions in the digestive tract and upper respiratory tract and have bactericidal and bactericidal effects against pathogenic microorganisms. We revealed that secretory immunoglobulin A and lactoferrin are also secreted in the sweat of the skin,
Furthermore, it was discovered that these have a selective growth inhibitory effect on Staphylococcus aureus among the indigenous epidermis. Therefore, it is presumed that Staphylococcus aureus is hardly observed in the skin-resident bacteria of healthy people.

Further, even in a healthy person, Staphylococcus aureus easily grows when dry, especially in winter, and the skin is apt to become rough. It is considered that this is because the amount of secreted immunoglobulin A and lactoferrin secreted on the skin is small due to the small amount of sweating. Therefore, it was confirmed that external use of this secretory immunoglobulin A and lactoferrin improves rough skin and the present invention has been completed.

[0006]

That is, the present invention is a cosmetic composition containing secretory immunoglobulin A and lactoferrin. The secretory immunoglobulin A used in the present invention is composed of two molecules of immunoglobulin A and a secretory compo-nent.
It is an immunoglobulin with a molecular weight of about 385,000 bound to J chain, which is contained in mucosal secretions and plays an important role in the biological defense mechanism as a local immune mechanism. Lactoferrin is a glycoprotein having a molecular weight of about 88,000 and exhibits a bactericidal effect by depriving bacteria of iron ions. Both secretory immunoglobulin A and lactoferrin are most abundant in human milk. In addition, it does not matter whether it is a human or an animal. This external preparation was separated and purified from this milk (Bennet R, et al .: A solid-phase radioimunoassay f
or the measurement of lactoferrin in human plasma:
Variations with age, sex, and disease. J Lab Clin
Med88: 156,1976) or a commercially available product can be used.

The amount of secretory immunoglobulin A and lactoferrin used in the cosmetic of the present invention is 0.0001 to 10% by weight, preferably 0.001 to 5% by weight, based on the total composition of each cosmetic. To be done. If it is less than 0.0001% by weight, the Staphylococcus aureus inhibitory effect is poor, and if it exceeds 10% by weight, it becomes difficult to maintain the stability of the product.

The cosmetics of the present invention include morphologically creams, lotions, emulsions, foundations, lipsticks, eye shadows, liquid hair styling products and the like.

[0009] The cosmetics of the present invention have the usual ingredients such as
Oily ingredients (eg squalane, petrolatum, olive oil, jojoba oil), surfactants (eg sorbitan sesquioleate, glyceryl monostearate, sodium lauryl sulfate), moisturizers (propylene glycol, glycerin), alcohols (cetyl alcohol) , Thickeners, preservatives, ultraviolet absorbers, colorants, fragrances and the like can be added.

[0010]

[Examples] Next, more detailed description will be given with reference to Examples.
The present invention is not limited to this. The blending amount is% by weight.

Example 1 Cream (1) Squalane 11.5 (2) Cetyl alcohol 2.5 (3) Polyoxyethylene (20) 1.0 Sorbitan monostearate (4) Polyoxyethylene (20) 2. 5 Cetyl ether (5) 1,3 Butylene glycol 4.0 (6) Propylene glycol 3.5 (7) Methyl paraoxybenzoate 0.03 (8) Secretory immunoglobulin A 3.0 (9) Lactoferrin 3.0 (10) Add purified water to make 100. Components (1) to (4) are dissolved by heating and mixed, and the mixture is kept at 70 ° C. to form an oil phase. Components (5) to (7) are uniformly dispersed in (10) and kept at 75 ° C to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify and disperse, and after cooling to 40 ° C., the component (8),
(9) is added and dissolved, and the product is cooled again to 30 ° C.

Example 2 Cream (1) Squalane 5.0 (2) Olive Oil 5.5 (3) Stearic Acid 3.0 (4) Beeswax 2.0 (5) Octyldodecyl Myristate 3.5 (6) Polyoxyethylene (20) 2.5 Cetyl ether (7) Behenyl alcohol 1.5 (8) Glycerin monostearate 2.5 (9) 1, 3 Butylene glycol 8.5 (10) Lactoferrin 0.1 (11) Secretion Immunoglobulin A 0.1 (12) Purified water is added to 100. The components (1) to (8) are dissolved by heating and mixed, and the mixture is kept at 70 ° C. to obtain an oil phase. The components (9) and (12) are kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify and disperse, and the mixture is cooled to 40 ° C., then the components (10) and (11) are added and dissolved, and the mixture is cooled to 30 ° C. again to obtain a product.

Example 3 Lipstick (1) Castor oil 45.0 (2) Olive alcohol 25.0 (3) Beeswax 5.0 (4) Paraffin wax 130F 10.2 (5) Candelilla wax 4.2 ( 6) Carnauba wax 2.2 (7) Titanium dioxide 2.0 (8) Red No. 202 0.5 (9) Red No. 204 2.5 (10) Red No. 227 A1 Lake 2.5 (11) Orange No. 201 0 .2 (12) Secretory immunoglobulin A 0.2 (13) Lactoferrin 0.3 (14) Perfume 0.2 Components (7) to (11) are dispersed in a part of the component (1) to form a pigment base. To do. Next, the components (2) to (6) and the rest of the component (1) are melted at 80 to 85 ° C., the pigment base and the components (12) to (14) are added, and the mixture is stirred, deaerated and put into a predetermined container. It is poured into and cooled and solidified to obtain a product.

Example 4 Milky liquid foundation (1) Stearic acid 2.4 (2) Monostearate propylene glycol 2.0 (3) Cetostearyl alcohol 0.2 (4) Liquid lanolin 2.0 (5) Fluid Paraffin 3.0 (6) Isopropyl myristate 8.5 (7) Butyl paraoxybenzoate 0.01 (8) Purified water 58.6 (9) Sodium carboxymethyl cellulose 0.2 (10) Bentonite 0.5 (11) Propylene glycol 4.0 (12) Triethanolamine 1.1 (13) Methyl paraoxybenzoate 0.02 (14) Titanium oxide 8.0 (15) Talc 4.0 (16) Color pigment 5.0 (17) Secretory immunoglobulin A 0.05 (18) Lactoferrin 0.05 (19) Perfume 0.1 component ( 0) is good swell component (8). The component (9) in which the component (11) is dispersed is added to and dissolved therein, and then the components (12) and (13) are dissolved to form an aqueous phase. Components (1) to (7) are melted by heating and kept at 80 ° C. to form an oil phase. Ingredients (1
Add 6), stir with homomixer and keep at 75 ° C. The oil phase is added to this aqueous phase while stirring and cooled, and the components (17) to (19) are added at 45 ° C., and the product is obtained after stirring and cooling.

[0015]

The secretory immunoglobulin A according to the present invention
The improvement effect of the cosmetics containing lactoferrin and lactoferrin on rough skin will be described with reference to test examples.

Experimental Example 1 Proliferation of human-derived secretory immunoglobulin A and lactoferrin (manufactured by Sigma) for three species of Staphylococcus aureus, Staphylococcus epidermidis, and Micrococcus luteurs 12708 of the genus Micrococcus The suppression effect was examined. The way is
1 mg of secretory immunoglobulin A and lactoferrin mixed in equal amounts of secretory immunoglobulin A and lactoferrin in tryptosoy broth medium (Eiken Chemical Co., Ltd.) inoculated with 10,000 bacteria per ml. The effect of inhibiting the growth of these three types of skin-resident bacteria was added and observed 3 and 6 hours after inoculation. as a result,
As shown in Table 1, the mixture of secretory immunoglobulin A and lactoferrin showed no increase in Staphylococcus aureus among the three kinds of bacteria tested, and therefore,
It was revealed that a mixture of secretory immunoglobulin A and lactoferrin has a selective growth inhibitory effect only on S. aureus.

[0017]

[Table 1] Proliferation amount: multiple of the number of bacteria at the time of inoculation sIgA = secretory IgA, LTF = lactoferrin A: Staphylococcus aureus B: Staphylococcus epidermidis C: Micrococcus

Experimental Example 2 A rough skin improvement test was carried out by using the cream of Example 1 for 10 days every day against 20 women with rough skin as a control. In the test, the cream of Example 1 was used on one side of the face by the half blind method, and the one from which secretory immunoglobulin A and lactoferrin were removed from Example 1 was used as a comparative control on one side. The judgment was made based on the degree of improvement compared with the control.

(Criteria for Judgment) 1. Remarkably improved. 2, improved. 3, slightly improved. 4. No improvement was observed.

[0020]

[Table 2] Table 2 Results ─────────────────── Judgment number of people ──────────────────── 14 (20%) 2 11 (55) 3 3 (15) 4 2 (10) ───────────────────

As shown in Table 2, the cream containing secretory immunoglobulin A and lactoferrin was found to have a skin roughening improving effect of 75%, and it was found that the cosmetic composition of the present invention is effective for rough skin.

[Safety] Primary irritation test in rabbits with 20% aqueous solutions of secretory immunoglobulin A and lactoferrin, sensitization test in guinea pigs, eyelid irritation test in rabbit eyes As a result, it was confirmed that no positive reaction was observed, and that it was safe.

Claims (1)

[Claims] 1. A cosmetic comprising a secretory immunoglobulin A and lactoferrin.