JPH08298888A - Fused hybrid mushroom from matsutake and shiitake mushrooms and its primordium cultivation and culturing process therefor - Google Patents

Abstract

PURPOSE: To economically mass-produce mushrooms of a fused fungus of MATSUTAKE fungus and SHIITAKE fungus by mix-inoculating MATSUTAKE fungi and SHIITAKE fungi in a desired culture medium at a prescribed ratio. CONSTITUTION: The saw dust of broad-leaf trees is mixed with clay and leaf mold, further vitamins and minerals are admixed thereto and the mixture is disinfected with a small amount of slaked line to prepare the culture medium. The powdery MATSUTAKE fungi and SHIITAKE fungi are inoculated to this culture medium at a desired ratio and kept at a room temperature ranging from 15 deg.C to 25 deg.C whereby the medium surface is unevenly covered with white mycelia. The cultivation is further continued and the medium is colored in dark brown on its surface. Then, the temperature in the cultivation chamber is kept at a temperature roughly less than 5 deg.C higher than the above-stated room temperature but lower than 28 deg.C to continue the cultivation for desired days to form primordia of the fungi. Further, the culture mixture is taken out, washed with water, transferred into the mycelia cultivator which is controlled to 20 deg.C for a few days whereby the mushrooms grow out of the culture medium surface breaking the coating membrane.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a fused mushroom of matsutake fungi and shiitake fungi, a method for culturing an primordia of the fused mushroom, and a cultivation method thereof.

[0002]

2. Description of the Related Art Conventionally, it has been impossible to say that a fused mushroom of matsutake fungus, which is a mycorrhizal fungus that parasitizes pine roots, and shiitake fungus, which has a deadly parasitic character.

[0003]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention The present inventor prays to obtain a mushroom that is a fusion of Matsutake fungi and Shiitake fungi, and to obtain a mushroom that can be easily cultured and can be artificially cultivated in a mass-produced manner. As a result of the experiment, we succeeded in obtaining this fused mushroom.
That is,

[0004]

[Means for Solving the Problems] Matsutake fungi and Shiitake fungi were mixed and inoculated into a required medium at a required ratio to obtain a fused mushroom of Matsutake fungi and Shiitake fungi. This fused mushroom has a lighter scent than real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, and the stem is thick and long with a good chewy texture and good taste.

In this fused mushroom of Matsutake fungi and Shiitake fungi, the mixing ratio of the aforementioned Matsutake fungi and Shiitake fungi is 2: 2 by weight.
It is preferably 8 to 5: 5. The matsutake fungi and the shiitake fungi may be in the form of powder or liquid.

The method of culturing the primordia of the fused mushroom of matsutake fungus and shiitake fungus of the present invention is applied to the sawdust of broad-leaved trees such as oak, oak, beech, and shii. Then 2
Commercially available white clay and humus (for each kg, 400 g in total) are mixed, and vitamins, minerals, sugars, etc. are added as nutrients, and a small amount of slaked lime is mixed and mixed. Together, they were put in a plastic bag and sterilized to form a medium, and the medium was inoculated with powdered Matsutake fungi and Shiitake fungi at a required ratio (2: 8 to 5: 5 by weight), and the room temperature was set to 15 ° to 25 ° C (preferably 18
The temperature of the medium is covered with white hyphae about 2 months after the temperature is kept at 20 to 20 ° C.), and the surface of the medium becomes uneven. After further culturing, the temperature of the medium becomes dark brown after about 3 months. It is higher than the room temperature by about 5 ℃ or less, but 28 ℃
Lower temperature (preferably 20 ° to 25 ° C)
It is said that mushroom primordia are formed on the surface of the medium by further culturing for the required number of days (about 2 weeks or more).

[0007] As described above, the method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungus of the present invention comprises mixing sawdust of broad-leaved wood with white clay and mulch soil, and adding vitamins, minerals, sugars, etc. Slaked lime is mixed and sterilized to form a medium, and the medium is inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio and kept at an indoor temperature of 15 to 25 ° C. It is covered with and irregularities are formed on the surface of the medium,
After further culturing, the surface of the medium becomes dark brown. After that, the room temperature is higher than the room temperature by about 5 ° C. or lower but lower than 28 ° C., and further cultivated for the required number of days. After the mushroom primordium is formed on the medium, the medium is taken out, washed with water, transferred to a mycelial culture room, and the room temperature is adjusted to 12 ° to 20 ° C (preferably 15 to 18 ° C) to form a film on the medium surface after several days. It is said that mushrooms are generated by breaking.

After the fused mushroom is generated as described above and grown to a required size in about 4 to 5 days, the fungus bed can be reused. That is, the bacterial bed after collecting the fused mushrooms is rested for about 2 weeks, then soaked in water for several days and drained, and the room temperature is set at 12 to 20 ° C.
By adjusting the temperature (preferably 15 to 18 ° C.) and culturing, mushrooms are generated again from the surface of the medium after several days (4 to 5 days). In this way, mushrooms can be obtained up to 3 times.

[0009]

According to the present invention, as described above, a fused mushroom of matsutake fungi and shiitake fungi is obtained for the first time. The scent is lighter than that of real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, with thick and long stems, sufficient fiber, and a good chewy texture and good taste. As for this fused matsutake mushroom, a small one can be obtained in an amount of 20 g to 30 g and a large one can be obtained in an amount of 50 g to 80 g. The yield is 300g from one bacterial bed
The harvest of 700g is expected and it is a new variety of mushrooms. If the air-conditioning facility is installed in the mushroom cultivation room, it can be cultivated throughout the year and the mushrooms can be harvested daily.

[0010]

EXAMPLE The fused mushroom of Matsutake and Shiitake mushrooms according to the present invention is as described above, and an example of the culturing and cultivating method of the primordium will be described below. Kunugi, oak, ▲
About 2 kg of sawdust (size 2 mm to 3 mm) of broad-leaved trees such as beech and shii (white bean), white soil (commercially available as Tomiyama Horticultural) and pine forest mullet are 400 g each in equal amounts. Mix rice and add to this as rice bran 2
00 g, sugar corn 20 to 30 g, bran 100 g, calcium chloride [CaCl ₂ .2H ₂ as food additive
O manufacturer Katayama Chemical Co., Ltd.] 5 g, oil cake 100
g, and 5-8 g of Ebios (manufactured by Asahi Breweries, Ltd.) as vitamin B are mixed, and slaked lime [Ca (O
H) ₂ ] 5 to 10 g are added, water is added, and the mixture is sufficiently stirred with a mixer and stored in a plastic container such as a plastic bag and sterilized under normal pressure,
The medium is naturally cooled to room temperature.

Next, the mouth of the plastic bag is opened, and powdered matsutake fungi and shiitake fungi are mixed and inoculated into the upper part of the medium at a weight ratio of 2: 8. As for the matsutake fungi and the shiitake fungi, the folds (folds) on the back of the umbrellas of the matsutake and shiitake were taken, and the hyphae were grown on an agar medium to which liquid glucose was added as a nutrient, and used.

Immediately after completion of the inoculation with the mushroom fungus, the mouth of the plastic bag is closed and the room temperature is maintained at 18 ° to 20 ° C. for culturing. After about 2 months, the medium was covered with white hyphae, and pit-shaped bumps and ridges were formed on the surface of the medium. After further culturing, the surface of the medium became dark brown after about 3 months. After this,
When culturing was carried out at a room temperature of 20 ° to 25 ° C, a mushroom primordium was formed on the surface of the medium in 3 weeks.

After the mushroom primordium is thus formed on the surface of the culture medium, the plastic bag is broken, the medium is taken out and washed with water to prepare for mushroom generation. That is, by moving to a mycelium culture room (bacterial bed culture shelf) and adjusting the room temperature to 15 to 18 ° C., one week later, the film was broken from the medium surface and mushrooms were generated.

After the fused mushrooms were generated as described above and grown to a required size in about 4 to 5 days, the fungal beds were reusable. That is, the bacterial bed after collecting the fused mushrooms was rested for about 2 weeks, soaked in water for 2 to 3 days, and drained, and the room temperature was set at 15 ° to 1 ° C.
By adjusting the temperature to 8 ° C. and culturing, mushrooms were generated again from the medium surface after 4 to 5 days. In this way, mushrooms were obtained up to 3 times. In the above-mentioned example, the fusion ratio of Matsutake fungi and Shiitake fungi was 2: 8, but the experiment in which the fusion ratio was 5: 5 was also conducted, and the same as the above except that the yield was reduced. Results were obtained.

[0015]

Industrial Applicability According to the present invention, as described above, a fused mushroom of Matsutake fungi and Shiitake fungi is obtained for the first time. The scent is lighter than that of real matsutake mushrooms, but its shape is similar to that of real matsutake mushrooms, with thick and long stems, sufficient fiber, and a good chewy texture and good taste. This fused matsutake mushroom is 20g to 30g for a small one and 50g to 80 for a large one.
g is also obtained. According to the method of the present invention, 300 g to 700 g can be harvested from one bacterial bed, and if an air conditioning facility is provided in the mushroom cultivation room, year-round cultivation can be performed and daily mushroom harvesting is possible. It has a great feature that it is suitable for mass production.

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[Procedure amendment]

[Submission date] July 14, 1995

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be amended] Claims

[Correction method] Change

[Correction content]

[Claims]

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0006

[Correction method] Change

[Correction content]

[0006] The method for cultivating a basal mushroom of fused mushroom of Shiitake fungus and Matsutake fungus of the present invention comprises inoculating the medium with Matsutake fungus and Shiitake fungus in a required ratio and keeping the room temperature at 15 to 25 ° C. Incubate until the medium is covered with white hyphae (preferably afterwards the room temperature is about 2 ° C to about 6 ° C higher than before but 28 ° C).
It is said that the temperature of the medium is lower than 0 ° C.), the surface of the medium becomes blackish brown in color, and a film is formed, and after further culturing for a required number of days, a mushroom primordium is formed on the surface of the medium. In addition,
The temperature range of 15 ° C. to 25 ° C. is set in this initial culture because the growth of mycelia becomes remarkably poor at 15 ° C. or lower, and the growth of mycelia may be inhibited at 25 ° C. or higher. . Further, by increasing the room temperature after the medium is covered with white hyphae by about 2 to 6 ° C, the growth of hyphae is promoted and the productivity is improved.
However, when the temperature is 29 ° C to 30 ° C or higher, mycelial growth is inhibited. As the medium, sawdust of broad-leaved trees such as oak, oak, oak, beech, and shii (for example, 2 kg) is commercially available for home gardening. A mixture of white clay and mullet (400 g in equal amount in total) is added to this to contain vitamins, minerals, sugars, etc. as nutrients, and a small amount of slaked lime is mixed and mixed in a plastic bag for sterilization. It is better to use a medium. The medium was inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio (weight ratio 2: 8 to 5: 5) and inoculated at a room temperature of 15 ° C.
After being kept at -25 ° C (preferably 18 ° C to 20 ° C), the medium is covered with white hyphae after about 2 months and the surface of the medium becomes uneven, and after further culturing, the surface of the medium becomes dark brown after about 3 months. After coloring, the room temperature is higher than the room temperature by about 5 ° C. or lower but lower than 28 ° C. (preferably 20 ° C. to 25 ° C.), and the required number of days (about 2 weeks). Above) By cultivation, mushroom primordia are formed on the surface of the medium.

[Procedure 3]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction content]

The method for cultivating a fused mushroom of matsutake fungus and shiitake bacterium of the present invention comprises inoculating the medium with matsutake bacterium and shiitake bacterium at a required ratio and keeping the room temperature at 15 to 25 ° C. Incubate until covered with white hyphae (preferably thereafter, by keeping the room temperature at about 2 ° C to about 6 ° C higher than before but lower than 28 ° C), the surface color of the medium is dark brown After a film is formed and the mushroom primordium is formed on the surface of the medium by further culturing for the required number of days, the medium is taken out, washed with water, and transferred to a mycelial culture room, where the room temperature is 12 to 20 ° C.
It is said that the mushroom is generated by breaking the film from the surface of the medium after several days by adjusting the temperature to ℃. Thus, as a medium, it is preferable to mix sawdust of hardwood with white clay and mulch and to add vitamins, minerals, sugars, etc., and to mix a small amount of slaked lime to sterilize the medium. . Powdered Matsutake fungi and Shiitake fungi are mixed and inoculated into the culture medium at a required ratio, and the culture medium is covered with white hyphae when the room temperature is maintained at 15 ° C to 25 ° C, resulting in unevenness on the culture medium surface. After the medium surface turns blackish brown, the room temperature is higher than the room temperature by about 5 ° C. or lower but lower than 28 ° C., and further cultivated for a required number of days so that the mushroom surface is not changed. After being formed, the medium is taken out and washed with water, and then transferred to a mycelial culture room, and the room temperature is kept at 12 ° C to 20 ° C.
By adjusting the temperature to a temperature (preferably 15 to 18 ° C.), mushrooms are generated by breaking the film from the surface of the medium after a few days.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Shogo Koishi 1-21-13, Shiohamacho, Hikoshima, Shimonoseki City

Claims (11)

[Claims] 1. A fused mushroom of matsutake fungi and shiitake fungi obtained by inoculating a required medium with matsutake fungi and shiitake fungi in a required ratio. 2. The fused mushroom of matsutake fungus and shiitake bacterium according to claim 1, wherein the fusion ratio of matsutake bacterium and shiitake bacterium is 2: 8 to 5: 5 by weight ratio. 3. Mixing white clay and mulch with hardwood sawdust,
In addition to containing vitamins, minerals, sugars, etc., this was mixed with a small amount of slaked lime and sterilized to form a medium, and the medium was inoculated with powdered matsutake fungi and shiitake fungi in the required proportions and the room temperature. After keeping the medium at 15 ° to 25 ° C, the medium is covered with white hyphae to form irregularities on the medium surface, and after further culturing, the medium surface becomes dark brown, and then the room temperature is about 5 ° C higher than the room temperature. A fused mushroom of Matsutake fungi and Shiitake fungi, characterized in that mushroom primordia are formed on the surface of the medium by culturing for a required number of days at a temperature below 28 ° C but below 28 ° C Primordium culture method 4. A mixture of white clay and mulch into hardwood sawdust,
In addition to containing vitamins, minerals, sugars, etc., this was mixed with a small amount of slaked lime and sterilized to form a medium, and the medium was inoculated with powdered matsutake fungi and shiitake fungi in the required proportions and the room temperature. After keeping the medium at 15 ° to 25 ° C, the medium is covered with white hyphae to form irregularities on the medium surface, and after further culturing, the medium surface becomes dark brown, and then the room temperature is about 5 ° C higher than the room temperature. After the temperature is higher than 28 ° C but lower than 28 ℃, after further culturing for a required number of days to form mushroom primordia, the medium is taken out, washed with water, transferred to a mycelial culture room, and the room temperature is kept at 12 ° C. ° ~ 20
A method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungus, characterized in that the mushroom is generated by breaking the film from the surface of the medium after several days by adjusting the temperature to ℃. 5. The method for cultivating a fused mushroom according to claim 4, resting the fungal bed after collecting mushrooms for about 2 weeks, soaking it in water for several days, and draining it to obtain the room temperature. A method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi, characterized in that mushrooms are generated again from the surface of the medium after several days by adjusting the temperature to 12 ° to 20 ° and culturing. 6. The method is characterized in that the room temperature is maintained at 18 ° C. to 20 ° C. when the medium is inoculated with powdered Matsutake fungi and Shiitake fungi in a required ratio and cultivated. The method for culturing a primordium of a fused mushroom of Pleurotus matsutake and Shiitake bacterium according to claim 3, 7. The room temperature for culturing until the medium surface turns blackish brown after the medium is covered with white hyphae and the medium surface has irregularities is set to 20 ° C. to 25 ° C. 4. A method for cultivating a primordium of a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 3, characterized in that 8. The room temperature at the time of culturing by mixing and inoculating the medium with powdered matsutake fungi and shiitake fungi in a required ratio is maintained at 18 ° C. to 20 ° C. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4, 9. The room temperature for culturing until the medium surface turns blackish brown after the medium is covered with white hyphae and the surface of the medium has irregularities is set to 20 ° C. to 25 ° C. 5. A method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4, characterized in that 10. The room temperature when the medium is taken out, washed with water and then transferred to a mycelium culturing site for culturing, after the mushroom primordium is formed on the surface of the medium is set to 15 ° C. to 18 ° C. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 4, 11. The room temperature when mushrooms are regenerated after collecting the mushrooms by the method for cultivating the fused mushrooms according to claim 4, wherein the room temperature is 15 ° C. to 18 ° C. The method for cultivating a fused mushroom of Matsutake fungi and Shiitake fungi according to claim 5.