JPH08208407A - Brown rot-controlling agent of cultured laver - Google Patents

Abstract

PURPOSE: To obtain a laver brown rot-controlling agent which is a combination of ketoglutaric acid with a specific acid, manifests high controlling effect in a short time without damage to cultured laver and can avoid environmental pollution because the controlling effect is almost equal to that using a combination of high concentration acids concentrated combination even if it is a combination of diluted acids. CONSTITUTION: This cultured laver brown rot-controlling agent is obtained by formulating at least one kind selected from the group consisting of adipic, succinic, monochloracetic, dichloracetic, trichloracetic, citric, gluconic, fumaric, formic, lactic, itaconic, phytic, phosphoric, hydrochloric, tartaric, sulfuric, nitric, maleic, malic, propionic, acetic, glutaric and butyric acids to an aqueous solution of ketoglutaric acid, and to provide a brown rot-controlling method.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a red rot fungicide for seaweed.

[0002]

2. Description of the Related Art In seaweed aquaculture, a method of acid treatment has been adopted in order to control miscellaneous algae, that is, Aonori, Ulva and Diatom. The price of seaweed will drop if aonori is mixed in the product. Various acids such as hydrochloric acid, phosphoric acid, citric acid, malic acid, phytic acid are used as these acids. Citric acid is mainly used because it is recognized as a food additive and is inexpensive.

1-2% of citric acid (pH about 2.0-2.
The processing of 5 to 10 minutes is performed in 2). In addition, it has been confirmed that a red rot disease is exterminating, and treatment with the above-mentioned concentration can exterminate within 1 to 2 minutes. Therefore, treatment is actually performed for 5 to 6 minutes.

A disease called red rot disease occurs rapidly when the seaweed fronds grow and can finally be harvested, and it spreads to all fishing grounds within a few days, causing the fronds to die and flow out. Therefore, the damage is great. The cause of this disease is said to be parasites of the genus Pythium or related fungi, and these microorganisms mainly grow at a temperature of around 15 ° C and are propagated by organisms or organic matter produced by the killing and decomposition of organisms. It is considered that the presence of a large amount of fronds on the sea surface satisfies the favorable growth conditions.

The red-rot fungus grows in a hyphae in the parasite,
The parasites feed and grow, and the vigorous hyphae form a large number of zoospores at the ends, resulting in a large number of zoospores in the sac. When the sac matures, it breaks and releases zoospores, which then swim in the water and attach to new parasites.
It becomes hyphae again and immediately eats parasite cells. It is normal for the damaged leaflets to be cut into pieces and fall off, which is said to be several hundred times as much damage as industrially directly eaten.

In Japanese Patent Publication No. Sho 46-35873, P-
There has been proposed a method for controlling erythema rot of red sea bream using oxybenzoic acid or its ester. This kills all bacteria, including useful bacteria, up to now with the use of antibiotics, reverse soaps, etc., but not only has a low ability to prevent mold and yeast, it is also harmless to humans. It depends on what is not.

Japanese Unexamined Patent Publication (Kokai) No. 50-12125 discloses a saturated fatty acid carboxylic acid having 1 to 4 carbon atoms, a saturated or unsaturated carboxylic acid having 2 to 4 carbon atoms, glycolic acid, lactic acid, tartaric acid, malic acid, citric acid. The algaecide containing 1 type, or 2 or more types of the organic carboxylic acid selected from is disclosed. Although this document exclusively describes the action of germicidal algae, there is no description of red-rot fungi.

Japanese Patent Publication No. 60-13647 discloses that it contains citric acid in an amount of 0.3 to 5.0% by weight and has a pH of 1.0 to 6.
Nori algae to be immersed in the treatment liquid of No. 0, extermination and prevention of disease are described.

In Japanese Patent Publication No. 60-13648, a pH of 1. is obtained by adding an inorganic acid such as hydrochloric acid, nitric acid, sulfuric acid or phosphoric acid.
A seaweed aquaculture method for controlling weeds and diseases, which is 0 to 4.0, is described.

JP-B-60-21950 discloses a fertilizer for seaweed cultivation containing phytic acid or a salt thereof as an active ingredient, a preventive effect against red rot disease, and a diatom exterminating effect.

Japanese Unexamined Patent Publication (Kokai) No. 57-8722 discloses a seaweed aquaculture method in which a seaweed net is treated with a treatment solution containing phosphoric acid or a salt thereof to remove and prevent diseases such as weeds and red rot. It is disclosed.

Japanese Unexamined Patent Publication (Kokai) No. 60-87202 discloses a fungicide for seawater bacteria of edible seaweed containing adipic acid as an active ingredient.

Japanese Unexamined Patent Publication (Kokai) No. 60-244245 discloses a method of algicidal treatment in which a fumaric acid in a saturated amount or more is present, and at least one of malic acid, citric acid, tartaric acid and succinic acid is used in combination. There is also a statement to the effect.

Japanese Patent Laid-Open No. 60-248121 discloses algae and acupuncture fungi by immersing in a treatment solution containing one or more of halogenated carboxylic acids such as chloroacetic acid, dichloroacetic acid and trichloroacetic acid. A seaweed aquaculture method for exterminating illness, red spot disease, etc. is disclosed.

Japanese Unexamined Patent Publication (Kokai) No. 62-21784 discloses a liquid fertilizer which is also used as a seaweed algaicide for laver cultivation, which is composed of an acidic buffer solution containing dilute hydrochloric acid as a main component, and nitrates, ammonium salts and phosphates.

Japanese Unexamined Patent Publication (Kokai) No. 62-190103 discloses a repellent for phytopathogenic fungi of the genus Pythium and Olpidiopsis, which is a causative agent of red rot and acupuncture fungus, containing gluconic acid as an active ingredient.

Japanese Unexamined Patent Publication (Kokai) No. 1-279805 discloses a repellent for algae and bacteria containing monosodium fumarate and / or monopotassium fumarate as an active ingredient.

Japanese Patent Application Laid-Open No. 2-25404 discloses a pesticide containing fumaric acid and / or fumarate and at least one selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, potassium carbonate and potassium hydrogen carbonate. Disinfectants for algae and bacteria which are dissolved in water or seawater to form monosodium fumarate and / or monopotassium fumarate are disclosed.

Japanese Unexamined Patent Publication No. 2-291218 (Japanese Patent Publication No.
(JP-A-47810), there is disclosed an algae killing method in which fumaric acid in an amount exceeding the saturated amount is allowed to exist in a state of being covered with a coating means having water permeability, and an object to be treated is dipped in the treatment liquid. .

As will be described later, the extermination of algae such as blue-green algae depends exclusively on the pH of the treatment solution. Therefore, the known hydrochloric acid, phosphoric acid, phytic acid, tartaric acid, citric acid, malic acid, Whether any acid such as succinic acid or gluconic acid is used alone or in combination, it is 9 to 9 at a pH of about 2.
You can get rid of it in 10 minutes.

[0021] However, since red rot fungi have a high infectivity when they are generated, they must be quickly treated to completely kill them. However, since infection from the sea occurs immediately, the seaweed will be destroyed at intervals of 2 to 6 days unless it is treated.

Nori producers average 100 to 200 seaweed nets on average. With the current treatment, it takes a minimum of 5 minutes to process 100 seams. However, since it takes 8 hours or more, 30 to 40 sheets can be processed per day. Therefore, as the spread of red rot becomes more severe, the control of red rot fungus is delayed, and the production of seaweed ends with no production of rotten seaweed. Therefore, there is a strong demand for the development of a drug capable of controlling red rot bacteria in a shorter time.

[0023]

SUMMARY OF THE INVENTION An object of the present invention is to provide a preparation capable of controlling red rot fungi in a short time without damaging the seaweed itself.

[0024]

[Means for Solving the Problems] As a result of intensive research to solve the above problems, it was found that ketoglutaric acid effectively acts on red-rot fungi and can be eliminated in an extremely short time. I have already applied for Japanese Patent Application No. 5-200843. As a result of further research, it was found that the combined use of ketoglutaric acid with adipic acid, lactic acid, phosphoric acid, etc. significantly increased the bactericidal activity of the acid itself, and also reduced the degree of damage to seaweed. Therefore, they have completed the present invention by finding that they can be treated at a low concentration for a short time and can reduce environmental pollution.

That is, the present invention is as follows. Aqueous solution of ketoglutaric acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid, fumaric acid, formic acid, lactic acid, itaconic acid, phytic acid, phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid, nitric acid And a red rot control agent for cultured seaweed, which comprises at least one selected from the group consisting of maleic acid, malic acid, propionic acid, acetic acid, glutaric acid, and butyric acid.

Currently, a muzzle boat has been developed and a short-time treatment (1
There is a fishing ground where treatment within minutes is performed, and by using ketoglutaric acid in combination with adipic acid, lactic acid, phosphoric acid, etc., it can be used at a low concentration and the amount of acid used can be reduced. In the case of use on a mobbing ship, if an inorganic acid such as hydrochloric acid or phosphoric acid is used, a concentration of 2 to 8% is required. Therefore, by using the present invention, the amount of acid used can be considerably reduced, and the environmental pollution load on the ocean can be reduced.

When ketoglutaric acid is used alone, 1
A concentration of 1.0% or more is required for controlling red-rot fungi in a treatment within minutes (shown in Japanese Patent Application No. 5-200843). As shown in the examples, ketoglutaric acid 0.
When it is less than 5%, it takes more than 1 minute to get rid of red rot bacteria.
By combining with other acids such as lactic acid and phosphoric acid, the treatment can be carried out in a shorter time, and the treatment agent can be sufficiently used even for the treatment within 1 minute.

The pesticide of the present invention comprises ammonium nitrate, ammonium chloride, monoammonium phosphate as a nitrogen source,
Urea, sodium nitrate, nitric acid and the like can be used in combination with 1 potassium phosphate, 1 sodium phosphate, inositol 6-phosphate phosphate and phosphoric acid as a phosphorus source. Particularly, when phosphoric acid and phosphate are used together, the damage of seaweed can be alleviated and soft seaweed can be produced.

The pesticide of the present invention is used by a method of immersing the seaweed culture net infected with red rot in the pesticide or by spraying the pesticide on the culture net. In this way, by contacting the laver that has been infected with the disease with the pesticide, the laver is not damaged and the disease-causing bacteria, adherent bacteria, diatoms, and algae are selectively exterminated. Treatment of aquaculture nets that do not contain disease is also carried out as a preventive measure.

[0030]

EXAMPLES The present invention will be described in more detail with reference to examples and comparative examples, but the present invention is not limited to these examples. In the following test examples,
The mark indicates that there is no disorder of laver and the red rot fungus is completely eradicated, and the symbol indicates that there is no disorder of laver and red rot is considerably suppressed, both of which are examples. is there.
Other marks are comparative examples.

Test Example 1 0.01 W of ketoglutaric acid
/ W% seawater solution, phytic acid (50%), phosphoric acid (7
5%), hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, itaconic acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, propionic acid, acetic acid, glutaric acid, Butyric acid 0.01, 0.05, 0.1, 0.2, 0.3
After dipping the seaweed thalli infected with red rot fungus for a certain period of time in the adjustment solution added to W / W%, wash it with sea water and observe the condition after 2 days of static culture under a microscope. The extermination effect was investigated. The evaluation is shown in Table 1, and the results are shown in Table 2
It shows in Table 5.

[0032]

[Table 1]

[0033]

[Table 2]

[0034]

[Table 3]

[0035]

[Table 4]

[0036]

[Table 5]

Test Example 2 0.1 W of ketoglutaric acid
Phytic acid (50%), phosphoric acid (75%) in W% seawater
%), Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, itaconic acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, propionic acid, acetic acid, glutaric acid, Butyric acid 0.01, 0.05, 0.1, 0.2, 0.3W respectively
/ W% was added to the adjusted solution to soak the seaweed thalli infected with red rot fungus for a certain period of time, then washed with sea water, and the condition after 2 days of static culture was observed under a microscope. The extermination effect was investigated. The results are shown in Tables 6 to 9.

[0038]

[Table 6]

[0039]

[Table 7]

[0040]

[Table 8]

[0041]

[Table 9]

Test Example 3 0.3 W of ketoglutaric acid /
Phytic acid (50%), phosphoric acid (75%) in W% seawater
%), Hydrochloric acid (36%), lactic acid (90%), formic acid (90
%), Tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, citric acid, gluconic acid, fumaric acid, itaconic acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, propionic acid, acetic acid, glutaric acid, Butyric acid, 0.01, 0.05, 0.1, 0.2, 0.3 respectively
After dipping the seaweed thalli infected with red rot fungus for a certain period of time in the adjustment solution added to have W / W%, wash with seawater,
The state after 2 days of static culture was observed under a microscope to investigate the effect of controlling red rot bacteria. The results are shown in Tables 10-13.

[0043]

[Table 10]

[0044]

[Table 11]

[0045]

[Table 12]

[0046]

[Table 13]

(Test Example 4) (Comparative Example) Phytic acid (50%), phosphoric acid (75%), hydrochloric acid (36%)
%), Lactic acid (90%) formic acid (90%), tartaric acid, sulfuric acid,
Nitric acid, maleic acid, malic acid, citric acid, gluconic acid,
Fumaric acid, itaconic acid, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, propionic acid, acetic acid, glutaric acid, butyric acid 0.01, 0.05, 0.
A 1, 0.2, 0.3 W / W% seawater solution was prepared, and the seaweed leaf bodies infected with red rot fungus were immersed for a certain period of time, then washed with seawater, and the state after 2 days of static culture was examined under a microscope. Observe at
The effect of controlling red rot fungus was investigated. The results are shown in Table 14 to Table 1.
7 shows.

[0048]

[Table 14]

[0049]

[Table 15]

[0050]

[Table 16]

[0051]

[Table 17]

(Test Example 5) Red sea rot-infected leaves were added to a seawater solution of the following combination obtained by adding two or more kinds of acids to ketoglutaric acid for 5 seconds, 10 seconds, 20 seconds ... 60 seconds, 90 seconds.
Second, 120 seconds immersion treatment, then wash with seawater and static culture 2
The condition after a day was observed under a microscope to investigate the effect of controlling red rot fungus. The combination formulations are shown in Table 18 below. Table 19 shows the control effect of the red rot fungus.

[0053]

[Table 18]

[0054]

[Table 19]

Test Example 6 Various acids used in combination with ketoglutaric acid in the present invention 0.01%, 0.05
%, 0.1%, 0.2%, 0.3%, when the ketoglutaric acid concentration was changed to 0%, 0.01%, 0.1%, 0.3%, red rot control The results of measuring the time are shown in Tables 20-23. It is clear that the extermination time of each type of acid is significantly shortened as compared with the case where ketoglutaric acid is not present.

[0056]

[Table 20]

[0057]

[Table 21]

[0058]

[Table 22]

[0059]

[Table 23]

[0060]

EFFECTS OF THE INVENTION By using ketoglutaric acid in combination with other acids such as adipic acid, succinic acid, lactic acid, phytic acid and phosphoric acid, the seaweed is not damaged and the bactericidal power of the acid itself is significantly increased, which is extremely short. Was able to exterminate the red-rot fungus.
Since the amount of acid used can be significantly reduced, environmental pollution to the ocean can be greatly reduced.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI technical display area A01N 59/26

Claims (2)

[Claims] 1. An aqueous solution of ketoglutaric acid is added to adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid, fumaric acid, formic acid, lactic acid, itaconic acid, phytic acid, phosphoric acid, hydrochloric acid, Tartaric acid,
An agent for controlling red rot of cultured seaweed, which comprises at least one selected from the group consisting of sulfuric acid, nitric acid, maleic acid, malic acid, propionic acid, acetic acid, glutaric acid, and butyric acid. 2. A ketoglutaric acid aqueous solution (including part or all of seawater) having a concentration of 0.3 W / W% or less, adipic acid, succinic acid, monochloroacetic acid, dichloroacetic acid, trichloroacetic acid, citric acid, gluconic acid. , Fumaric acid, formic acid, lactic acid, itaconic acid, phytic acid, phosphoric acid, hydrochloric acid, tartaric acid, sulfuric acid, nitric acid, maleic acid, malic acid, propionic acid, acetic acid, glutaric acid, butyric acid, at least one selected from the group consisting of Acid concentration of 0.01 to 0.3 W /
A method for exterminating a disease of cultured seaweed characterized by immersing the cultured seaweed in this mixed solution or spraying this solution on the cultured seaweed, by setting the W% range corresponding to the required treatment time.