JPH08205855A - Medium - Google Patents

Abstract

PURPOSE: To obtain a powder medium capable of omitting labor of weighing the powder medium in preparing a medium, preventing the powder medium from scattering, facilitating throwing the powder medium into a dissolving container, further checking moisture absorption and preventing moisture absorption during preservation after opening a packing material. CONSTITUTION: This medium is obtained by separately packing a powder medium in an amount for use for once, optionally packing doubly the powder medium with a moisture-resistant packing material and making the moisture-resistant packing material resealable. The powder medium is prepared by sealing and packing a fixed volume of a powder medium as a liquid medium or an agar medium with a packing material having moisture resistance. The medium is obtained by sealing and packing plural packed bags prepared by packing a fixed volume of the powder medium as a liquid medium or an agar medium with a water vapor-resistant packing material. The medium is obtained by sealing and packing a fixed volume of a powder medium as a liquid medium or an agar medium with a water-soluble film having thermally fusing properties and sealing and packing one or plural bags of the sealed and packaged bags with a moisture-resistant packing material.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a culture medium. More specifically, the present invention relates to a medium that does not require weighing of the medium when producing the medium.

[0002]

2. Description of the Related Art A typical medium for culturing microorganisms, cells, plants and the like is a liquid medium used in a liquid state and an agar medium obtained by solidifying the liquid medium with agar. Generally, these media are commercially available as powdered media in metal containers, plastic containers, or bags, although these media vary depending on the type of the media. Then, at the time of use, it is necessary to weigh out a required amount of powder medium according to the amount of liquid medium or agar medium required, and after weighing, dissolve in a predetermined amount of water, and most of the medium is further sterilized. To use from.

However, since the powder medium is fine particles,
There is a problem that it scatters at the time of weighing, stains the scale and the laboratory table, and scatters when the powder medium is put into a container for dissolving the powder medium. This problem has been solved by changing the powder medium from fine particles to particles having a large particle size in some culture media, but many culture media remain conventional fine particles.
Furthermore, if the container for dissolving the powdered medium is a container with a narrow opening such as an Erlenmeyer flask, the powdered medium adheres to the opening at the time of charging or spills out of the container, which may cause a problem that the container cannot be charged well. is there.

The commercially available powder medium is 100
It is often packaged in units of g or multiples of it, but when actually used it is 100 ml as a liquid medium or agar medium.
Since the powder medium is weighed (not a multiple of 100 g) so as to be 1 liter (liter) or the like, when the powder medium is newly opened, it is almost always left unused.
However, since the powder medium has a high hygroscopic property, the powder medium left over after the opening absorbs moisture during storage, and even if there is no quality problem, it is difficult to weigh it or dissolve it in water during subsequent use. There is a problem of becoming.

That is, it suffices if there is a powder medium packaged only for one time use, but such a medium has not yet been marketed.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above problems, and saves the labor of weighing the powder medium at the time of producing the medium and can prevent the powder medium from scattering, It is an object of the present invention to provide a powdery medium that can be easily put into a container for dissolving a medium and can prevent moisture absorption during storage after opening.

[0007]

[Means for Solving the Problems] In order to solve the above-mentioned problems, according to the present invention, a powder medium is packaged in each usage amount and, if necessary, double-packed with a moisture-proof packaging material. ,
What makes the moisture-proof packaging material resealable is a medium in which a fixed volume of a powder medium as a liquid medium or an agar medium is hermetically packed with a moisture-proof packaging material, and a liquid medium or agar medium. Is a culture medium in which a plurality of packaging bags for packaging a fixed volume of powder medium are sealed and packaged with a moisture-proof packaging material, and a fixed volume of powder medium as a liquid medium or agar medium is heat-fusible. It is a culture medium which is hermetically packaged with a water-soluble film having, and one or more of which are hermetically packaged with a moisture-proof packaging material.

The present invention will be described in detail below.

[0009] Generally, when a medium is produced in an Erlenmeyer flask, a Sakaguchi flask, etc., the production amount is 100 per container.
It is often produced in 100 ml units such as ml and 200 ml. On the other hand, in the case of a large capacity such as a fermentation culture tank, it is often produced in a unit of liter (l). In addition, a flask with a volume of 500 ml or 1 l is often used, and the amount of medium produced is mainly 10 to 30% of the volume of the liquid medium and 50 to 80% of the agar medium. There is almost no limit to the final volume of the medium, which is determined by each person.

Therefore, for example, in a powder medium for liquid medium, 100 ml, 200 ml, 30 ml of liquid medium are used.
The required amount of the medium can be obtained by packaging 0 ml and 1 l of the powdered medium as one batch and using them in combination as needed. For agar medium, 300 ml as agar medium, 4
00 ml, 500 ml, 1 liter of powder medium for each 1
It can be packaged as a batch. The amount of the powder medium to be packaged as one batch does not have to be limited to the above amount and can be freely determined according to the use conditions.

The powder medium may be packaged by measuring the amount used once and packaging with a plastic film, and the method is not particularly limited. Also, the material of the plastic film to be used may be a single film or a laminated film as long as it can be heat-sealed, and any film in terms of material can be used, but it prevents moisture absorption during storage. Therefore, a laminated film containing a moisture-proof material such as an aluminum foil, an aluminum vapor deposition film, or a metal oxide vapor deposition film is preferable.

When the plastic film for packaging the powder medium is not moisture-proof, a plurality of these packaging bags may be double-wrapped with a moisture-proof packaging material. In that case, the moisture-proof packaging material needs to have a re-sealing property in order to prevent moisture absorption of the remaining portion. Specifically, for example, as shown in FIGS. 1 and 2, two laminated films (1) (1) containing the above-mentioned moisture-proof material are overlapped and the side edges are sealed (2). A bag, a metal can, or the like may be used, and in the case of a bag, a resealing mechanism (hereinafter referred to as a chuck) (3) for fitting a convex portion and a concave portion as shown in FIGS. 1 and 2 may be provided. When a container such as a metal can is used, the main body and the lid may be fitted to each other to be resealed.

As a packaging material for the powder medium, a water-soluble film can be used as long as it has a heat-sealing property. As such a film, there is a film containing polyvinyl alcohol as a main component. However, when a water-soluble film is used, since the water-soluble film does not have moisture resistance, it is necessary to double-wrap even a single bag with a moisture-proof packaging material. The double wrapping method may be performed in the same way as the above-mentioned method, but when double wrapping only one bag, it will be used up once, that is, it is not necessary to store it, so it is necessary to add resealability. There is no.

The powder medium may be packed in any form, but it is necessary to make it easy to use.
Specifically, in the case where a water-soluble film is not used, in order to facilitate loading into a container having a narrow opening such as a flask, a stick-like packaging form as shown in FIG. ) A straight line or V
It is preferable to impart easy opening property to the bag, such as by providing a notch (5) such as a letter shape. Also, when using a water-soluble film, it is necessary to make it into a size and shape that can be directly put into a flask or the like.

The packaged powder medium is opened in one bag or a plurality of bags according to the amount of the medium prepared at the time of use, the powder medium is put into a container such as a flask or a fermentation tank, and then the usual medium preparation method is used. The medium may be prepared by the same method. If it is packaged with a water-soluble film, it may be placed in the container for producing the medium as it is without opening it. In this case, the water-soluble film is dissolved in water for dissolving the medium together with the powdered medium which is the content, does not remain as a foreign substance, and does not affect the components (microorganisms, cells) to be cultured, which is a problem. It doesn't.

There is no limitation on the type of target medium, and the present invention can be applied to all mediums handled as powder.

[0017]

As described above, according to the present invention, the powder medium is packaged in a quantity to be used once, so that it is easy to put it in a medium-producing container such as a flask without weighing it, and it is left unused. do not do. Further, in the case where a plurality of packages are collectively packaged, moisture resistance during storage is maintained by imparting resealability to the packaging material for double packaging. Further, in the case of hermetically packaging with a water-soluble film, it is not necessary to open the flask when it is charged into the flask, and it can be directly charged into the container for medium preparation without opening.

[0018]

【Example】

<Example 1> 18.0 g of powdered normal broth medium was made up of polyethylene terephthalate (PET) / adhesive / adhesive / aluminum foil (AL) / polyethylene (PE) 4
The bag was hermetically sealed in a bag of 0 mm × 100 mm, and a V-shaped cut was made for opening. Powdered normal broth medium 18.0
g corresponds to 1000 ml of the liquid medium. The packaged powdered medium was stored at room temperature for 6 months and no moisture absorption occurred. In addition, the two bags were opened, and the powder medium was put into a fermentation culture tank having a volume of 3000 ml to obtain 2000 m.
Although an ordinary broth medium (1) was prepared, the solubility in water and the performance as a medium (growth of microorganisms) were the same as those of the conventional medium, and there was no problem at all.

<Example 2> Powdered tryptic soy broth medium was hermetically packed in a stick-shaped bag made of expanded polypropylene (OPP) / PE and having the shape shown in Fig. 3, and the four bag sizes and powders shown in Table 1 were used. A combination of medium filling amounts was prepared, and a linear cut was made in the bag for opening. These bags were further packed with OPP /
Adhesive / Aluminum evaporation unstretched polypropylene (CP
P), which is a double-packing bag having a zipper having the shape shown in FIG. In addition, 3.0 g of powdered tryptic soy broth medium was used as 10 g of the liquid medium.
It corresponds to 0 ml. Each double package was opened, and each one bag of the powder medium was taken out, resealed with a zipper and stored at room temperature for 6 months, but no moisture absorption of the powder medium was observed. After 6 months, 100 ml each in a 500 ml flask using the packed powder medium,
200 ml of tryptic soy broth medium was added,
300 ml and 400 m in a 00 ml flask, respectively.
Although the tryptosoy broth medium of 1 was prepared, it was easy to put the powder medium into the flask, the solubility in water, the performance as a medium (growth of microorganisms) was equivalent to that of the conventional medium,
There was no problem.

[0020]

[Table 1]

Example 3 15.6 g of powdered potato dextrose agar (PDA) medium was added to 25 mm × 100 mm.
Stick-shaped polyvinyl alcohol (PVA) film (Hiselon S type manufactured by Nigo Film Co., Ltd.)
After wrapping with the bag, the size of OPP /
The package was hermetically sealed in a bag of adhesive / silicon oxide vapor deposition CPP. In addition, 15.6 g of powdered PDA medium was used as agar medium 4
Equivalent to 00 ml. This double-wrapped powder medium is
Almost no moisture was absorbed at room temperature for 6 months. Also, two bags of this powder medium together with a bag of PVA were put into a 1000 ml flask to prepare an 800 ml agar medium.
The film was completely dissolved and had no effect on the properties of the PDA medium.

<Example 4> 4.7 g of powdered standard agar medium
After packaging (corresponding to 200 ml of agar medium) in a 15 mm × 80 mm stick-shaped bag made of the same PVA film as used in Example 3, the 100 bags were put into a steel can with a lid having a diameter of 120 mm and a height of 200 mm. It was placed in two layers and stored at room temperature. Two bags were put into a 500 ml flask every two days, and 400 ml of the standard agar medium could be used up without moisture absorption until the end.

[0023]

EFFECTS OF THE INVENTION The medium of the present invention does not need to be weighed at the time of use because the powder medium is packaged for each standard amount of use. Further, in the case of a small volume, since the packaging form is a stick, the powder medium can be easily charged into a container having a narrow opening such as a flask. Furthermore, since a moisture-proof material is used as the packaging material, it is possible to prevent moisture absorption during storage.

Further, by making the packaging material of the powder medium a water-soluble material, the whole bag can be put into a container for medium preparation, and not only the weighing but also the opening of the bag is unnecessary.

As described above, the culture medium of the present invention is a powder medium having excellent handling properties as compared with the conventional powder medium.

[0026]

[Brief description of drawings]

FIG. 1 is a front view showing an example of the shape of a bag for double packaging when performing double packaging of the present invention.

FIG. 2 is an explanatory view showing an example of a re-sealing mechanism for a double-wrapping bag when performing double-wrapping according to the present invention.

FIG. 3 is an explanatory view showing an example of a packaging form of the powder medium of the present invention.

[Explanation of symbols]

 1 Laminated film 2 Seal part 3 Re-sealing mechanism 4 Vertical seal part 5 Notch

 ─────────────────────────────────────────────────── ─── Continued Front Page (72) Inventor Toshimi Yamamoto 1-5-1 Taito, Taito-ku, Tokyo Toppan Printing Co., Ltd.

Claims (5)

[Claims] 1. A medium in which a fixed volume of a powder medium as a liquid medium or an agar medium is hermetically packed with a moisture-proof packaging material. 2. A medium in which a plurality of packaging bags in which a fixed amount of powder medium as a liquid medium or an agar medium is packaged are hermetically packaged with a moisture-proof packaging material. 3. A fixed volume of a powder medium as a liquid medium or an agar medium is hermetically packaged with a water-soluble film having a heat-sealing property, and one or more bags thereof are hermetically packaged with a moisture-proof packaging material. Culture medium. 4. The culture medium according to claim 3, wherein the water-soluble film having a heat-fusing property is a film containing polyvinyl alcohol as a main component. 5. The culture medium according to claim 1, wherein the moisture-proof packaging material has a resealing property.