JPH0782136A - Pack base and its production - Google Patents

Abstract

PURPOSE:To produce an excellent pack base containing activities of enzymes such as protease and lipase, useful for beautification of skin from the extraction residue of a plant ash which had not no use hitherto. CONSTITUTION:A cultured mixture obtained by adding an aqueous solution of acetic acid or brewed vinegar to ash obtained by ashing a plat and neutralizing the ash, inoculating Aspergillus oryzae yeast into the residue obtained when the resultant mineral component is extracted into a solution layer and then culturing the yeast is used as this back base.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a culture obtained by inoculating and culturing a residue obtained when a plant is incinerated by adding an acetic acid aqueous solution to the ash to neutralize and extract mineral components into a solution layer. It relates to the utilization of objects as a pack base.

[0002]

2. Description of the Related Art Mineral solutions obtained by neutralizing ash obtained by ashing plants with aqueous acetic acid and extracting mineral components into a solution layer are commercially available as a rejuvenating / revitalizing agent for vegetables etc. Kosho 61-8721).

In the field of cosmetics, among proteases and carbohydrate-degrading enzymes, those having good stability are used for facial cleansers, packs and the like. In addition, various amino acids are used with the expectation of the effect of restoring hydration to the aged or hardened epidermis (Tetsusaku Ikeda, Ed., "Cosmetics" 13th edition, Nanzandou, 1978, p. 178). ). It is also known that kojic acid and its analogs obtained by culturing microorganisms of the genus Aspergillus and the like exhibit a melanin production inhibitory effect and can be used as a fair skin cosmetic (JP-A-53-3538, JP-B-53-3538). 56-18569, other related patent applications).

[0004]

One of the objects to be solved by the present invention is to add a large amount of acetic acid aqueous solution to ash obtained by ashing a plant to neutralize and extract a large amount of mineral components into a solution layer. It is to make effective use of the generated residue. Furthermore, it was aimed to develop an excellent pack base by utilizing the residue.

[0005]

[Means for Solving the Problems] When an aqueous acetic acid solution is added to ash obtained by ashing a plant to neutralize and extract mineral components into a solution layer, if the supernatant is to be left standing, Liquid separation requires a long period of about 2 weeks (Japanese Patent Publication No. 61-87).
21, page 10, right column, top line 10). That is, ash neutralized by adding an aqueous acetic acid solution contains an extremely fine solid substance. It has not been possible to analyze in detail what the solid material is, but it is presumably predominantly a silicate such as kaolinite which is insoluble in acetic acid.

[0006] It was found that molds grow when the solid substance obtained by allowing to stand and separating as described above is left at room temperature.
Although it is a natural phenomenon to consider that the residual acetic acid is assimilated, it was found that the ash residue can be used as a culture medium for microorganisms, and the idea for achieving the present invention was obtained. Hereinafter, detailed description will be given according to each step of claim 1.

(A) Step of separating undissolved residue from plant ash: The plant ash used as a raw material for the pack base of the present invention may be either grass or wood, but industrially, it is a by-product during charcoal production. It is easy to obtain the charcoal ash and the ash of the oil palm empty bunch that is incinerated at the palm oil manufacturing plant. 1-8% to the ash
Neutralize by adding the acetic acid aqueous solution or brewed vinegar mentioned above until carbon dioxide gas is not generated. Separation of the solution layer and the solid layer (undissolved residue) of the reaction product may be carried out by any method such as decantation, filtration and centrifugation. For example, if the reaction product is sieved with a sieve of 200 to 300 mesh and the section passing through the sieve is centrifuged to separate the solid layer, the particle size is 2
A mud-like residue with a uniform particle size of about 10 μm can be obtained.

(B) Step of inoculating and culturing Koji bacterium: To the residue obtained in (a) above, a calculated amount of commercially available kaolin is added to adjust the water content to 50 to 60%, and an aqueous acetic acid solution is added to the extract. When used, it is supplemented with a nitrogen source such as about 0.1% (ratio to dry matter) of sodium nitrate and ammonium sulfate and mixed. When using brewed vinegar, it is not necessary to supplement the nitrogen source. Then, the mixture is sterilized and cooled at 120 ° C. for 15 minutes, and spores of Aspergillus oryzae are sprayed, and then placed in an incubator (a tray with an aluminum lid or an automatic koji making machine) to a thickness of about 2 to 3 cm, and 30 ° C. Culture at. The microorganisms used here are Aspergillus flavus ( Aspe
rgillus oryzae ) is preferred. This is because commercially available seed koji is easily available and has excellent safety. Among the commercially available seed koji, those having a strong neutral protease activity, and further, a mutant strain having a white spore (conidia) color may be selected and used. The culture period is 5 to 6 days. When the culture period is extended to 2-3 weeks, ferric salt coloring substances such as kojic acid increase (Shuichi Kanda et al., Journal of Japan Brewing Vol. 86, 884).
~ P. 885, 1991).

(C) Drying / powdering step: The above culture is vacuum dried at 60 ° C. or lower and pulverized into a fine powder. Dry
For crushing and sieving, a commonly used method is appropriately combined.

[0010]

【Example】

Example 1: 10 kg of charcoal ash produced as a by-product during the production of charcoal was added with stirring a 5% aqueous solution of acetic acid to neutralize carbon dioxide gas until it no longer generated. The neutralized solution was passed through a 200-mesh screen to remove coarse contaminants, and the mixture was centrifuged to obtain 12.4 kg of a solid layer (undissolved residue) (water content 59%). Kaolin (2 kg) and sodium nitrate (10 g) were added to and mixed with the mixture (water content adjusted to about 50%). The mixture was placed in an autoclave and sterilized at 120 ° C for 15 minutes. After cooling down, seed koji 1
An aluminum tray (60 x
90 cm) was spread and accommodated, and it was placed in a thermostatic chamber at 30 ° C.
After 6 days of culturing, the product was taken out from the tray, dried, pulverized and sieved in the usual manner to obtain 6.4 kg of a pack base. The enzyme activity of the pack base was as follows, and the color reaction with ferric chloride was weak. Neutral protease 2500 units / g Lipase 360 units / g [Measurement method and enzyme unit] Protease was measured by the Folin method at pH 7.0, and the tyrosine equivalent amount was 1 mmol / min as 1 unit. The pH of lipase is measured by the alkali titration method.
Measured at 7.0, 1 micromol fatty acid liberation / min
The unit was used. (Edited by Michio Kozaki, "Handbook of Enzyme Utilization", Jijijinkan, 1980)

Example 2: 3 kg of ash obtained by incinerating an empty bunch of oil palm was neutralized and dissolved by adding brewed vinegar, and after centrifugation, this time, as in Example 1, without adding a nitrogen source of sodium nitrate. To give 1.3 Kg of the pack base. Enzyme activity is protease 4300 units / g, lipase 83
It was 0 unit / g.

Example 3: An example of a formulation for producing a powdered pack product using the pack bases shown in Examples 1 and 2 is shown below. <Prescription example> Pack base of the present invention 50.0% Talc 20.0 Zinc white 19.0 Olive oil 2.0 Polyoxyethylene (40 mol) sorbitan monolauric acid ester 1.0 Propylene glycol 8.0 Fragrance Suitable Amount Preservative Appropriate amount The above product is kneaded with lotion, emulsion, fruit juice, honey, etc. according to the user's skin condition or preference, and applied as a paste. At that time, it is also effective to use a commercially available enzyme drink (for example, trade name, Bioteam manufactured by Japan Biological Science Institute Co., Ltd.) as the water for kneading.

[0013]

As described above, according to the present invention,
An excellent pack base can be produced from the residue of the plant ash extracted with an aqueous solution of acetic acid, which has not been discarded until now.
Further, by inoculating and culturing the extract residue with Koji bacteria, enzyme activities such as protease and lipase which are useful for beautifying the skin can be contained, and the acetic acid odor of the residue is eliminated so that the pack base It can also have a scent of Koji which is preferable to many Japanese.

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Claims (3)

[Claims] 1. A method for producing a pack base, which comprises combining the following steps. (A) A step of ashing the plant, neutralizing the ash by adding an aqueous solution of acetic acid to extract the mineral components into a solution layer, and separating the residue (b) A step of inoculating and culturing the residue with Aspergillus oryzae (C) Step of drying and powdering the culture of Koji bacteria 2. The method for producing a pack base according to claim 1, wherein the acetic acid aqueous solution is brewed vinegar. 3. A pack base produced by the production method according to claim 1.