JPH06505562A - 組換えヒト組織因子及び精製された天然及び合成のリン脂質からのプロトロンビン時間試薬の製造 - Google Patents
組換えヒト組織因子及び精製された天然及び合成のリン脂質からのプロトロンビン時間試薬の製造Info
- Publication number
- JPH06505562A JPH06505562A JP5507008A JP50700893A JPH06505562A JP H06505562 A JPH06505562 A JP H06505562A JP 5507008 A JP5507008 A JP 5507008A JP 50700893 A JP50700893 A JP 50700893A JP H06505562 A JPH06505562 A JP H06505562A
- Authority
- JP
- Japan
- Prior art keywords
- tissue factor
- reagent
- prothrombin time
- phospholipid
- reagents
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1277—Processes for preparing; Proliposomes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/745—Assays involving non-enzymic blood coagulation factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/04—Phospholipids, i.e. phosphoglycerides
Abstract
Description
Claims (7)
- 1.プロトロンビン時間試薬であって、(a)ヒト組織因子のアミノ酸配列を実 質的に有する組換え蛋白質と、 (b)前記蛋白質を活性化するに十分な量のリン脂質と、(c)緩衝剤組成物と 、そして (d)該組換え蛋白質を活性化するに十分な量のカルシウムイオンとからなるも のである、プロトロンビン時間試薬。
- 2.プロトロンビン時間試薬であって、(a)ヒト組織因子の細胞質部分に実質 的に対応するアミノ酸配列を有する組換え蛋白質と、 (b)前記蛋白質を活性化するに十分な量のリン脂質と、(c)緩衝剤組成物と 、そして (d)該組換え蛋白質を活性化するに十分な量のカルシウムイオンとからなるも のである、プロトロンビン時間試薬。
- 3.プロトロンビン時間試薬であって、(a)ヒト組織因子のアミノ酸配列を実 質的に有する組換え蛋白質と、 (b)前記蛋白質を活性化するに十分な量のリン脂質混合物と、(c)緩衝剤組 成物と、そして (d)該組換え蛋白質を活性化するに十分な量のカルシウムイオンとからなるも のである、プロトロンビン時間試薬。
- 4.該リン脂質がフォスファチジルコリン及びフォスファチジルセリンよりなる 群より選ばれるものである、請求項1又は2に記載のプロトロンビン時間試薬。
- 5.リン脂質の該混合物がフォスファチジルコリン及びフォスファチジルセリン よりなる群より選ばれるものである、請求項3に記載のプロトロンビン時間試薬 。
- 6.該リン脂質が合成のものである、請求項1又は2に記載のプロトロンビン時 間試薬。
- 7.組織因子を含有する脂質ミセルを製造するための方法であって、 (a)該リン脂質を界面活性剤中に溶解させ、(b)組織因子を、該溶解させた リン脂質と組み合せ、(c)組み合わされたリン脂質及び組織因子混合物を、接 線流システム中に、前記混合物が前記システムの膜と接触するような方法で置き 、そして (d)該システムを緩衝液で洗い流すことよりなる方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US77129491A | 1991-10-04 | 1991-10-04 | |
US771,294 | 1991-10-04 | ||
PCT/US1992/008281 WO1993007492A1 (en) | 1991-10-04 | 1992-09-25 | Preparation of prothrombin time reagents from recombinant human tissue factor and purified natural and synthetic phospholipids |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH06505562A true JPH06505562A (ja) | 1994-06-23 |
JP3416778B2 JP3416778B2 (ja) | 2003-06-16 |
Family
ID=25091357
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50700893A Expired - Lifetime JP3416778B2 (ja) | 1991-10-04 | 1992-09-25 | 組換えヒト組織因子及び精製された天然及び合成のリン脂質からのプロトロンビン時間試薬の製造 |
Country Status (11)
Country | Link |
---|---|
US (1) | US5625036A (ja) |
EP (1) | EP0565665B1 (ja) |
JP (1) | JP3416778B2 (ja) |
AT (1) | ATE163768T1 (ja) |
AU (1) | AU663343B2 (ja) |
CA (1) | CA2097199C (ja) |
DE (1) | DE69224622T2 (ja) |
DK (1) | DK0565665T3 (ja) |
ES (1) | ES2115679T3 (ja) |
NZ (1) | NZ244597A (ja) |
WO (1) | WO1993007492A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003516525A (ja) * | 1999-12-10 | 2003-05-13 | デイド・ベーリング・インコーポレイテッド | 予め作製されたリポソームへの精製膜蛋白質の再構成 |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5418141A (en) * | 1994-05-06 | 1995-05-23 | Avocet Medical, Inc. | Test articles for performing dry reagent prothrombin time assays |
IL115849A0 (en) * | 1994-11-03 | 1996-01-31 | Merz & Co Gmbh & Co | Tangential filtration preparation of liposomal drugs and liposome product thereof |
AT403437B (de) * | 1995-02-15 | 1998-02-25 | Immuno Ag | Physiologisch aktive gewebefaktor-präparation |
CA2236155A1 (en) * | 1995-11-22 | 1997-05-29 | Oklahoma Medical Research Foundation | Assay for blood clotting factors viii and ix |
US5858724A (en) * | 1996-07-16 | 1999-01-12 | Pel-Freez | Recombinant rabbit tissue factor |
US6379975B1 (en) | 1996-11-27 | 2002-04-30 | T.A.C. Thrombosis And Coagulation Aktiebolag | Methods and reagents for determining protein S |
DE69832628T2 (de) * | 1997-04-23 | 2006-09-07 | Instrumentation Laboratory S.P.A. | Rekombinant-kaninchengewebefaktor basiertes prothrombinzeitreagenz |
AU9114598A (en) * | 1997-08-26 | 1999-03-16 | University Of North Carolina At Chapel Hill, The | Method of monitoring blood low molecular weight heparin and heparin |
US6245573B1 (en) | 1998-02-12 | 2001-06-12 | University Of Medicine And Dentistry Of New Jersey | Rapid assessment of the coagulant activity of blood |
EP1031830B8 (fr) | 1999-02-23 | 2009-12-09 | Asulab S.A. | Système électrochimique pour la détermination d'un temps de coagulation du sang |
US6733985B1 (en) * | 1999-05-19 | 2004-05-11 | International Technidyne Corporation | Preparation of stable liquid and dried synthetic prothrombin time reagents |
US6699718B1 (en) * | 1999-09-03 | 2004-03-02 | Roche Diagnostics Corporation | Method, reagent and test cartridge for determining clotting time |
US6800298B1 (en) * | 2000-05-11 | 2004-10-05 | Clemson University | Biological lubricant composition and method of applying lubricant composition |
US6743596B1 (en) | 2000-10-27 | 2004-06-01 | Biomerieux, Inc. | Method of determining global coagulability hemostatic potential |
US6645768B1 (en) * | 2000-10-27 | 2003-11-11 | Biomerieux, Inc. | Reagent and kit for determining global coagulability and hemostatic potential |
US6855509B2 (en) | 2000-12-19 | 2005-02-15 | Instrumentation Laboratory Company | Protein S functional assay and kit therefor |
US6596543B2 (en) | 2001-03-22 | 2003-07-22 | Dade Behring Inc. | Use of liposomes of defined composition and size for the preparation of prothrombin time reagents |
US7358337B2 (en) * | 2002-08-16 | 2008-04-15 | International Technidyne Corporation | Assay for low molecular weight heparin |
US7049087B2 (en) | 2002-11-05 | 2006-05-23 | Lifescan, Inc. | Method for manufacturing a tissue factor-based prothrombin time reagent |
AU2003282351A1 (en) * | 2002-11-12 | 2004-06-03 | Inverness Medical Switzerland Gmbh | Photometric determination of coagulation time in undiluted whole blood |
US7604932B2 (en) * | 2002-11-13 | 2009-10-20 | Haematologic Technologies, Inc. | Assay for tissue factor in a sample |
US20050221414A1 (en) * | 2004-03-31 | 2005-10-06 | Katalin Varadi | Kit for measuring the thrombin generation in a sample of a patient's blood or plasma |
US7148067B2 (en) * | 2004-08-31 | 2006-12-12 | The Board Of Trustees Of The University Of Illinois | Thromboplastin reagents |
US20080260858A1 (en) * | 2005-02-16 | 2008-10-23 | The Board Of Trustees Of The University Of Illnois | Universal Procoagulant |
AT501650A1 (de) * | 2005-02-22 | 2006-10-15 | Technoclone Ges M B H | Verfahren zur bestimmung der gerinnungsaktivierung sowie gerät zur durchführung des verfahrens |
DE602006021290D1 (de) | 2005-03-04 | 2011-05-26 | Univ Illinois | Modulator von coagulationskaskaden und fibrinolytischen kaskaden |
JP5073270B2 (ja) | 2006-11-14 | 2012-11-14 | シスメックス株式会社 | 凝固検査用試薬及びその製造方法 |
EP2040073A1 (en) * | 2007-09-20 | 2009-03-25 | Iline Microsystems, S.L. | Microfluidic device and method for fluid clotting time determination |
US8821861B2 (en) | 2007-10-05 | 2014-09-02 | The Board Of Trustees Of The University Of Illinois | Fibrin sealant |
US20100297257A1 (en) * | 2007-11-09 | 2010-11-25 | National Institutes Of Health (Nih), U.S. Dept. Of Health And Human Services (Dhhs) | Anticoagulant antagonist and hemophillia procoagulant |
WO2011144953A1 (en) * | 2010-05-18 | 2011-11-24 | Diagon Kft. | Procedure for producing recombinant human tissue factor |
CN103003441A (zh) | 2010-05-28 | 2013-03-27 | 戴阿冈有限公司 | 脂质体的两相制备方法及其在制造诊断试剂中的应用 |
CN102608337B (zh) * | 2011-04-22 | 2014-05-14 | 武汉塞力斯生物技术有限公司 | 凝血酶原时间测定试剂盒及其制备方法 |
CN112481355B (zh) * | 2020-11-16 | 2023-05-30 | 武汉市长立生物技术有限责任公司 | 液体型凝血酶原时间测定试剂盒及其制备方法 |
CN115508569A (zh) * | 2022-09-15 | 2022-12-23 | 上海太阳生物技术有限公司 | 蛋白s试剂及检测试剂盒 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4289498A (en) * | 1979-01-08 | 1981-09-15 | Ortho Diagnostics, Inc. | One-stage prothrombin assay and compositions useful therein |
US4461833A (en) * | 1982-06-23 | 1984-07-24 | University Patents, Inc. | Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract |
US4721572A (en) * | 1985-07-12 | 1988-01-26 | Miles Laboratories, Inc. | Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices |
SE458342B (sv) * | 1987-07-06 | 1989-03-20 | Alfa Laval Ab | Centrifugalseparator innefattande en rotor med en separeringskammare bestaaende av tvaa avdelningar |
US4865984A (en) * | 1988-02-08 | 1989-09-12 | Mount Sinai School Of Medicine Of The City University Of New York | Dynamic continuous flow enzyme reactor |
US5472850A (en) * | 1991-04-10 | 1995-12-05 | Oklahoma Medical Research Foundation | Quantitative clotting assay for activated factor VII |
SE464135B (sv) * | 1989-07-14 | 1991-03-11 | Kabivitrum Ab | Foerfarande foer bestaemning av funktionell aktivitet av fritt protein s eller protein c i ett plasmaprov |
US5314695A (en) * | 1990-11-13 | 1994-05-24 | Corvas International, Inc. | Tissue factor based prothrombin time reagent |
-
1992
- 1992-09-25 JP JP50700893A patent/JP3416778B2/ja not_active Expired - Lifetime
- 1992-09-25 AT AT92921544T patent/ATE163768T1/de active
- 1992-09-25 AU AU27692/92A patent/AU663343B2/en not_active Expired
- 1992-09-25 DE DE69224622T patent/DE69224622T2/de not_active Expired - Lifetime
- 1992-09-25 DK DK92921544T patent/DK0565665T3/da active
- 1992-09-25 CA CA002097199A patent/CA2097199C/en not_active Expired - Lifetime
- 1992-09-25 WO PCT/US1992/008281 patent/WO1993007492A1/en active IP Right Grant
- 1992-09-25 EP EP92921544A patent/EP0565665B1/en not_active Expired - Lifetime
- 1992-09-25 ES ES92921544T patent/ES2115679T3/es not_active Expired - Lifetime
- 1992-10-02 NZ NZ244597A patent/NZ244597A/xx not_active IP Right Cessation
-
1995
- 1995-01-10 US US08/371,052 patent/US5625036A/en not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003516525A (ja) * | 1999-12-10 | 2003-05-13 | デイド・ベーリング・インコーポレイテッド | 予め作製されたリポソームへの精製膜蛋白質の再構成 |
Also Published As
Publication number | Publication date |
---|---|
CA2097199C (en) | 2001-05-08 |
EP0565665A1 (en) | 1993-10-20 |
JP3416778B2 (ja) | 2003-06-16 |
US5625036A (en) | 1997-04-29 |
DE69224622D1 (de) | 1998-04-09 |
WO1993007492A1 (en) | 1993-04-15 |
DE69224622T2 (de) | 1998-11-05 |
AU663343B2 (en) | 1995-10-05 |
DK0565665T3 (da) | 1998-09-28 |
CA2097199A1 (en) | 1993-04-05 |
NZ244597A (en) | 1993-09-27 |
AU2769292A (en) | 1993-05-03 |
ATE163768T1 (de) | 1998-03-15 |
ES2115679T3 (es) | 1998-07-01 |
EP0565665B1 (en) | 1998-03-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPH06505562A (ja) | 組換えヒト組織因子及び精製された天然及び合成のリン脂質からのプロトロンビン時間試薬の製造 | |
DE60012785T2 (de) | Rekonstruktion gereinigter membranproteine in vorgefertigten liposomen | |
KR100196107B1 (ko) | 조직인자를 베이스로 하는 프로트롬빈시간 시약 및 그 제조방법 | |
ES2255157T3 (es) | Reactivo de tiempo de protrombina basado en el factor tisular recombinante de conejo. | |
Hong et al. | Modulation of membrane fusion by calcium-binding proteins | |
WO2006031387A1 (en) | Thromboplastin reagents | |
JPS61142467A (ja) | 脂質診断学用の管理血清もしくは検定用血清及びその製造法 | |
US6596543B2 (en) | Use of liposomes of defined composition and size for the preparation of prothrombin time reagents | |
EP1185690A1 (en) | Preparation of stable liquid and dried synthetic prothrombin time reagents | |
EP3225991B1 (en) | Reagent for prothrombin time measurement, method for production thereof, and method for measurement of prothrombin time | |
Tans et al. | Lipid phase transitions and procoagulant activity | |
US6183979B1 (en) | Preparation of dried synthetic prothrombin time reagents | |
Fernández-Montalván et al. | μ-Calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation | |
CA2112192A1 (en) | Process for reactivating purified membrane proteins | |
Esmon et al. | Lupus anticoagulants, thrombosis and the protein C system | |
Mayer et al. | Membrane lateral phase separation induced by proteins of the prothrombinase complex | |
JP3993649B2 (ja) | 精製された膜タンパク質の凍結による再活性化方法 | |
JP2008088103A (ja) | Ii、vii、ix、x因子測定用試薬及びその製造方法 | |
Vardhanabhuti et al. | Preparation of liposomes with asymmetric distribution of phosphyatidylinositol 4, 5-bisphosphate across the bilayer |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090411 Year of fee payment: 6 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090411 Year of fee payment: 6 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313111 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090411 Year of fee payment: 6 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090411 Year of fee payment: 6 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100411 Year of fee payment: 7 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110411 Year of fee payment: 8 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120411 Year of fee payment: 9 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120411 Year of fee payment: 9 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130411 Year of fee payment: 10 |
|
EXPY | Cancellation because of completion of term | ||
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20130411 Year of fee payment: 10 |