JPH0648915A - External agent composition for ultraviolet protection and wrinkle prevention - Google Patents

Abstract

PURPOSE:To provide an external agent composition compounded with a water- insoluble elastin, exhibiting excellent ultraviolet-protection effect and resultant wrinkle-preventing effect, having high safety and useful for cream, milky lotion, face lotion, foundation, etc. CONSTITUTION:The external agent composition having excellent ultraviolet- protection effect and wrinkle-preventing effect and having high safety is produced by compounding a water-insoluble elastin obtained e.g. by extracting bovine connective tissue with an alkali or a modifying agent such as guanidine- hydrochloric acid. The amount of the water-insoluble elastin in the composition is preferably about 0.01-10% based on the total composition. The composition can be used in the form of cream, milky lotion, face lotion, foundation, ointment, etc. The composition can be properly compounded with water-soluble or oil- soluble base, a preservative, a perfume, a pH modifier, etc., according to the form of the agent.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly safe external preparation composition having an excellent UV protection effect and a resulting wrinkle prevention effect by incorporating water-insoluble elastin.

[0002]

2. Description of the Related Art As human skin ages, wrinkles and sagging occur due to atrophy and diminished elasticity. It is generally said that aging progresses by two factors. One is controlled by the internal factor of the cell, that is, genetic factor, which is called endogenous aging or physiological aging, and the other is the influence of external factors, such as nutrition, environment, endocrine and stress. It is caused by and is called extrinsic aging or pathological aging.

Recently, attention has been paid to the harmfulness of ultraviolet rays, and attention has been paid to the idea that ultraviolet rays are one of the external factors that promote aging of the skin. When observing changes in the skin due to long-term exposure to sunlight, the skin becomes dry,
It can be seen that it has a diffusely brownish tone and increases in thickness, forming stiff wrinkles.

In the skin exposed to sunlight for a long period of time, especially ultraviolet rays, elastic fibers (consisting of elastin protein) originally present in the dermis are abnormally increased, and so-called sunlight elastic fibrosis changes in the skin occur. This increase in elastic fibers mainly appears in the upper layer of the dermis, and when the degree of exposure to ultraviolet rays is strong, not only the increase in these fibers but also degeneration into lumps covers the upper layer of the dermis widely. Such changes have been considered to have been caused only by the degeneration and increase of elastic fibers by ultraviolet rays, but the present inventors have taken a step further and thought that this change does not allow ultraviolet rays to enter deeper than this part of the dermis. He had the idea that he was acting as a barrier. This idea is similar to the idea that ultraviolet rays increase melanin in the epidermis, but this is because the increased melanin prevents ultraviolet rays from entering deeper skin (dermis). Such changes in elastic fibers are convenient for preventing the deep penetration of ultraviolet rays, but they also have a bad appearance in that the skin becomes rough and deep wrinkles are formed.

Therefore, the present inventor has thought that the ultraviolet rays can be blocked by covering the skin surface with water-insoluble elastin, which is a component of the elastic fiber. That is, water-insoluble elastin, which is an elastic fiber that increases as a biological reaction upon exposure to ultraviolet rays, is applied to the surface of the skin in advance to block the ultraviolet rays, thereby preventing the ultraviolet rays from entering not only the epidermis but also the dermis. Increase in elastic fibers,
It is intended to prevent the appearance of deep wrinkles caused by this.

With respect to the prevention or treatment of wrinkles, the application of natural antioxidants such as α-tocopherol and β-carotene, scavengers for free radicals (peroxides), ultraviolet scattering agents and absorbents is known. . Further, in recent years, it has been reported that retinoic ascid has a remarkable effect of improving wrinkles. However, the effects of the cosmetics containing these substances are not sufficient, and there is a problem in safety, which is not satisfactory.

[0007]

Means for Solving the Problems The present inventor believes that these changes in the skin tissue are one of the biological defense responses to sun exposure, and if elastin is applied to the skin, deep skin (dermis) due to ultraviolet rays It was thought that the protein denaturation does not occur and wrinkles can be prevented, and as a result of intensive studies, it was found that wrinkles due to ultraviolet rays can be prevented by applying water-insoluble elastin, and the present invention was completed.

That is, the present invention relates to a UV protection and wrinkle external preparation composition which is characterized by containing water-insoluble elastin, is excellent in UV protection and wrinkle prevention effects, and is highly safe.

The water-insoluble elastin used in the present invention is obtained from connective tissue by alkali extraction, a method using an autoclave, an extraction using a denaturing agent such as guanidine-hydrochloric acid, and the like. It can be confirmed by amino acid analysis, presence of cross-linking due to lysine residue, and the like. The water-insoluble elastin is crushed by a crusher (jet mill) and classified to a predetermined particle size. The particle size of the water-insoluble elastin used in the present invention is in the range of 0.1 to 150 μm, preferably 0.5 to 50 μm. Production Example 1 shows a preparation example of water-insoluble elastin.

Production Example 1 Preparation of Water-Insoluble Elastin 100 g of bovine connective tissue is heated in 0.1 N NaOH 101 at 95 ° C. for 45 minutes. The reaction solution is filtered, and the filter paper residue is washed three times with water. Then, it is dried with a mixed solution of alcohol and ether. The mixing ratio of alcohol and ether is gradually increased from 90:10, and finally ether alone is dried. After drying, ether is distilled off to obtain about 10 g of water-insoluble elastin. Then, it was pulverized to produce water-insoluble elastin having an average particle size of 5 μm.

The amount of water-insoluble elastin to be added to the wrinkle-prevention external preparation composition of the present invention is not particularly limited, but it is preferably 0.01 to 10% based on the total amount of the final composition. If it is less than 0.01%, the effect is not sufficiently exhibited, and 1
Even if the content is 0% or more, the effect corresponding to the content cannot be obtained.

Examples of dosage forms to which the present invention can be applied include creams, emulsions, lotions, foundations, ointments and the like. The UV protection and anti-wrinkle external preparation composition of the present invention can be appropriately blended with a water-soluble and oil-soluble base, a preservative, a perfume, a pH adjuster, etc., depending on the dosage form, within a range to achieve the purpose. .

[0013]

The present invention will be described in detail below with reference to examples and comparative examples. The present invention is not limited to the examples below. Unless otherwise specified,% in the examples is% by weight.
Indicates. The UV protection test, wrinkle test and safety test in the examples were carried out by the following methods.

(Ultraviolet protection test) 1) Ultraviolet irradiation test After applying a sample (0.2 mg / cm ² ) to the back of a hairless mouse, 6 MED of UVB was irradiated with Toshiba FL-20 · SE to observe the occurrence of erythema. . 2) Ultraviolet transmission test A sample (0.2 mg / cm ² ) was applied to freeze-dried pig skin, which is said to be close to human skin, and the skin permeability of ultraviolet rays was measured with a monochromator manufactured by JASCO Corporation.

(Wrinkle prevention test) The back of the mouse was shaved, and 2 days a day
Ultraviolet rays were irradiated for a period of time and one month, and the presence or absence of a wrinkle preventive effect was judged by visual observation.

(Safety test) A practical test was conducted by 20 test subjects. As the test method, the subjects were asked to normally use the sample, and the number of subjects who had an itch such as itchiness and redness immediately after application was compared.

Example 1 Cream (composition) (%) 1. Liquid paraffin 15.0 2. Vaseline 8.0 3. Monoguri 3.0 4. POE sorbitan monooleate 2.0 5. Glycerin 10.0 6. Sodium lauryl sulfate 1.0 7. Water-insoluble elastin of Production Example 1.0 1.0 8. Water 60.0 (Production method) 1.1 to 4 are uniformly dissolved at about 80 ° C. 11.5 to 8 are uniformly mixed and dispersed at about 80 ° C. After II is added to I and emulsified, the mixture is cooled to room temperature to obtain the UV protection and wrinkle cream of the present invention.

Comparative Example 1 Cream A cream for comparison is obtained in the same manner as in Example 1 except that 1.0% of water-insoluble elastin is replaced with water.

Comparative Example 2 Cream 1.0% water-insoluble elastin was added to α-tocopherol 1.
A cream for comparison is obtained in the same manner as in Example 1 except that 0% is substituted.

Comparative Example 3 Cream Prepared in the same manner as in Example 1 except that 1.0% of water-insoluble elastin was replaced with 1.0% of retinoic acid,
Get a cream for comparison.

With respect to the creams of Example 1 and Comparative Examples 1 to 3, the above-mentioned ultraviolet protection test, wrinkle test and safety test were carried out. As a result, in the ultraviolet protection test 1), Example 1 did not cause erythema and showed a considerably excellent blocking effect. Also, in the ultraviolet protection test 2), as shown in FIG. 1, UVB (wavelength 290 to 320 nm) and UVA
A blocking effect was observed at both wavelengths (320 to 400 nm). Furthermore, in the wrinkle prevention test, a remarkable wrinkle prevention effect was observed, and no wrinkle was generated even after irradiation with ultraviolet rays for one month.

As a result of the wrinkle test, Comparative Example 1 containing no water-insoluble elastin and 1.0% of α-tocopherol
Comparative Example 2 in which was added had no wrinkle-preventing effect, and wrinkles were observed to occur one month after UV irradiation. Retinoic assid 1.
Comparative Example 3 containing 0% had the same wrinkle-preventing effect as in Example 1, but in the safety test, 16 subjects complained of symptoms such as redness, itching, and thickening of the skin, which was a safety problem. was there. Regarding Example 1 and Comparative Examples 1 and 2, there were no symptoms that caused a problem in the safety test.

Example 2 Lotion (composition) (%) 1. Ethyl alcohol 8.0 2. POE hydrogenated castor oil 1.0 3. Fragrance 0.1 4. Paraben 0.1 5. Glycerin 10.0 6. Citric acid 0.02 7. Sodium citrate 0.08 8. Water-insoluble elastin of Production Example 0.1 9. Water 80.6 (manufacturing method) I. 1 to 4 are uniformly mixed and dissolved. II. 5 to 7 and 9 are mixed and dissolved uniformly. After adding II to I and mixing, 8 is uniformly mixed and dispersed,
A lotion of the present invention of the type in which water-insoluble elastin floats is obtained.

Example 3 Foundation (composition) (%) 1. Titanium oxide 10.0 2. Talc 42.0 3. Mica 31.0 4. Yellow iron oxide 3.0 5. Red iron oxide 1.0 6. Carbon black 0.5 7. 7. Water-insoluble elastin of Production Example 10.0 8. Squalane 2.0 9. Paraben 0.5 (Production method) 1 to 7 are uniformly mixed, then 8, 9 are added, further mixed and die-molded to obtain the foundation of the present invention.

Example 4 Ointment (composition) (%) 1. Vaseline 60.0 2. Polyethylene glycol 4000 25.0 3. Polyethylene glycol 400 13.0 4. Water-insoluble elastin 2.0 of Production Example 1 (Production method) 1 to 3 were dissolved under heating, and 4 was kneaded.
Cool to room temperature to obtain the ointment of the present invention. Examples 2 to
As a result of performing various tests on No. 4 in the same manner as in Example 1, all exhibited excellent ultraviolet ray protection effect and wrinkle prevention effect, and there was no particular problem in safety.

[0026]

Industrial Applicability As described above, it is clear that the ultraviolet protection and wrinkle external preparation composition of the present invention has excellent ultraviolet protection and wrinkle protection effects and high safety.

[0027]

[Brief description of drawings]

FIG. 1 shows the results of UV transmittance of each cream of Example 1 and Comparative Example 1.

Claims (1)

[Claims] 1. A composition for external use for UV protection and wrinkles, which comprises water-insoluble elastin.