JPH06181636A - Growth activating agent for lawn grass - Google Patents

Abstract

PURPOSE:To obtain a growth activating agent for lawn grass free from danger which exerts adverse influence upon human body and natural circumstance by use and consisting essentially of natural raw materials and a method for activating growth of lawn grass using a growth activating agent of lawn grass free from danger which exerts adverse influence upon human body and natural circumstance. CONSTITUTION:(1) A growth activating agent for lawn grass comprising a chitosan-decomposed material and acetic acid as active ingredients, (2) a growth activating agent for lawn grass comprising a chitosan-decomposed material, acetic acid and alfalfa green leaf deprotein supernatant as active ingredients and (3) a method for activating lawn grass using the activating agent of these lawn grasses.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a lawn grass activator comprising a chitosan degradation product and acetic acid or an alfalfa green leaf deprotein supernatant as an active ingredient as a third ingredient, or a method for activating such lawn grass. .

[0002]

2. Description of the Related Art At present, it is widely practiced to spray a growth activator on turf grass for the purpose of curing the turf grass. However, the majority of such growth activators often contain an ingredient that is harmful to the human body and the natural environment. Therefore, when spraying this onto turf grass on a golf course, for example, it had to be sprayed in a form that takes into consideration the human body and the natural environment. Moreover, even if such a form of spraying is performed, it cannot be denied that there is some adverse effect on the human body and the natural environment.

[0003] Despite such circumstances, a lawn grass growth activator whose active ingredient has no possibility of adversely affecting the human body and the natural environment has not yet been found.

[0004]

SUMMARY OF THE INVENTION Therefore, an object of the present invention is to provide a growth activator for turfgrass, which is mainly composed of a natural material which does not have a harmful effect on the human body and the natural environment, and an adverse effect on the human body and the natural environment. It is intended to provide a method for activating the growth of turfgrass using a turfgrass growth activator which is not dangerous.

[0005]

Means for Solving the Problems As a result of intensive studies to solve the above problems, the present inventor has decomposed chitosan, a growth activator for turfgrass, characterized by containing a decomposed product of chitosan and acetic acid as active ingredients. , Acetic acid, and alfalfa green leaf deproteinization supernatant are contained as active ingredients.The lawn grass growth activator is mainly composed of natural materials that have no risk of adversely affecting the human body or the natural environment when used. The present invention has been completed by discovering that it is a growth activator for turfgrass. Furthermore, the present invention also provides a method for activating lawn grass growth using these turf grass growth activators.

The present invention will be described in detail below. (1) The turfgrass growth activator of the present invention has a synergistic growth-promoting effect when a chitosan degradation product having a plant growth-promoting effect and acetic acid (synthetic acetic acid, acetic acid-containing solution, vinegar, etc.) are mixed and used in turfgrass. It was discovered by focusing on the increase. Chitosan is deacetylated chitin contained in crustaceans such as shrimp and crab and cell walls of microorganisms, and also has a low degree of polymerization obtained by degrading chitosan (hereinafter referred to as a chitosan degradation product). Are known.

It is known that chitosan and chitosan degradation products promote the growth of plants [Journal of the Japanese Society of Agricultural Chemistry, Vol. 62, No. 8, pages 1238 to 1240 (1988), Polymer Science Technology, Vol. 38, pages 45 to 59 (1988)]. In practical terms, a seed germination promoting method using low molecular weight chitin or chitosan (Japanese Patent Laid-Open No. 3-19
8702) has been proposed. The decomposed product of chitosan used in the present invention is obtained by decomposing the above-mentioned chitosan into an appropriate molecular weight using an acid or an enzyme. The acid used for acid decomposition of chitosan may be either an organic acid or an inorganic acid. Examples of organic acids include acetic acid, lactic acid, citric acid, malic acid and the like; examples of inorganic acids include hydrochloric acid, sulfuric acid and the like.

The enzyme used for enzymatic decomposition is
Examples thereof include chitosanase, papain, cellulase, acid protease, pectinase and the like. The acid decomposition method of chitosan is illustrated in JP-A 1-128775 and the like, and the enzymatic decomposition of chitosan is illustrated in JP-A 1-291799 and the like. Chitosan degradation products obtained by these methods can be used in the present invention. The degree of decomposition of chitosan is not particularly limited, but those having a molecular weight of about 10,000 to 50,000 are preferable.

Next, acetic acid used in the present invention is a concept including not only synthetic acetic acid and acetic acid-containing liquid but also vinegar and the like.
An example of the acetic acid-containing liquid is the vinegar solution described in Japanese Patent No. 959222. Using such a vinegar solution contains acetate in addition to acetic acid,
It is preferable in that it causes less chemical damage than the case of using synthetic acetic acid or vinegar.

The amount of the acetic acid added to the growth activator of the turfgrass of the present invention is 0.25 to 1 part by weight of the above-mentioned chitosan decomposition product.
It is about 4 parts by weight, preferably about 1 part by weight. In addition, when acetic acid is added, it is preferable to increase the pH so that the pH is as close to neutral as possible in the pH range where the decomposition product of chitosan does not precipitate, because the chemical damage can be suppressed. Specifically, it is usually preferable to adjust the pH to around 5. (2) Furthermore, by adding alfalfa green leaf deproteinization supernatant as the third component, the growth activating effect of turfgrass is further enhanced.

The alfalfa green leaf deproteinization supernatant (hereinafter referred to as "brown juice") used in the present invention is obtained by heating and removing proteins from alfalfa which is used as feed or food. Flat 1-
No. 191397. Regarding the utilization of such brown juice in plants, utilization as a fertilizer and the like has been tried so far, but utilization as a lawn grass growth activator has not been studied at all. The amount of the brown juice blended in the turfgrass growth activator of the present invention is 2 to
It is about 20% by weight, preferably about 10% by weight.

The turfgrass growth activator of the present invention is used in the form of a liquid, powder, granule or the like, with the addition of a suitable carrier and the like. Moreover, you may add a transfer agent as needed. There are various usage methods and usage concentrations of this growth activator, but in the case of the liquid agent, the concentration of acetic acid is adjusted at the time of use to be about 0.002 to 0.2% by weight, and the spraying period for spraying on turfgrass is usually 5 to 5. 20
It may be determined every day while observing the growth activation effect of turfgrass.

Finally, the kind of turfgrass for which the turfgrass growth activator of the present invention can be used is not particularly limited. Specific examples thereof include, but are not limited to, Korseishiba, bentgrass, ryegrass, and the like.

[0014]

[Example 1]

(1) Preparation of chitosan decomposition product Dissolve 60 g of chitosan (manufactured by Tokyo Kasei Kogyo Co., Ltd.) in 1 L of a 3.0 wt% acetic acid solution to prepare a chitosan solution at 121 ° C.
It was hydrolyzed by heating under pressure for 60 minutes. Then, the pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan decomposition product (A). (2) Preparation of turfgrass growth activator of the present invention 5 parts by weight of the chitosan degradation product (A) obtained in (1) above, 2 parts by weight of vinegar having an acetic acid concentration of 15% by weight and 2 parts by weight of pure water are mixed, A lawn grass activator (A) was obtained. (3) Preparation of medium containing chitosan degradation product and acetic acid Chitosan degradation product obtained in (1) above in 1/2 concentration MS medium
(A) (added 1/2000 parts by weight), vinegar having an acetic acid concentration of 15% by weight (added 1/5000 parts by weight), and the lawn grass growth activator (A) obtained in (2) above. ) (Each containing 1/1000 parts by weight) is added, the pH is adjusted to 5.8 with sodium hydroxide, and 10 ml is dispensed into each test tube.
It was autoclaved at 15 ° C for 15 minutes. (4) Germination test Bentgrass (variety name: Highland Bent) seeds were sterilized with a sodium hypochlorite solution (in Table 1, Group I was treated for 15 minutes, Group II was treated for 10 minutes), and sterilized one by one Seeded. After that, germination and growth were performed under the dark condition at 25 ° C. The germination rate 30 days after sowing was investigated. The results are shown in Table 1. As is clear from the table, a germination promoting effect was observed in the chitosan degradation product (A) and the turfgrass growth activator-added group. Moreover, since the germination promoting effect was greater in the turfgrass growth activator-added group, it was clarified that the germination promoting effect of the chitosan degradation product was increased in the presence of acetic acid.

[0015]

[Table 1]

Example 2 (1) Preparation of Degradation Product of Chitosan 6.0% of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 0.2N hydrochloric acid.
The mixture was dissolved by mixing so as to have a weight percentage, and this was used as a chitosan solution, and heated and heated at 100 ° C. for 120 minutes for hydrolysis. Then, the pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan decomposition product (B). (2) Preparation of acetic acid-containing liquid To 1 L of brewed vinegar having an acetic acid concentration of 10% by weight, 10 g of eggshell, 7.0 g of sodium bicarbonate and 3.33 g of sodium hydroxide were added and mixed. The acetic acid concentration of this product was 8% by weight. (3) Preparation of brown juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at 130 ° C is fed into the juice to coagulate the protein, followed by centrifugation for the purpose. I got brown juice. The details of the preparation process are as follows.
The description was made in JP-A-04-53416. (4) Preparation of lawn grass growth activator of the present invention 5 parts by weight of the chitosan degradation product (B) obtained in (1) above, 4 parts by weight of the acetic acid-containing solution obtained in (2) above and 2 parts by weight of pure water were mixed. Then, a lawn grass growth activator (B) was obtained.

Further, the chitosan decomposition product obtained in the above (1)
(B) 5 parts by weight, and 4 parts by weight of the acetic acid-containing solution obtained in (2) above.
And 2 parts by weight of the brown juice obtained in (3) above.
Then, the lawn grass growth activator (C) was obtained. (5) Growth promotion test Hard soil is placed in a plastic pot (18 cm in diameter and 22 cm in depth).
Fill with 5 g of perennial ryegrass (Lolium perenn
e) Seeds were sown and covered with vermiculite. Sowing
From the 9th day after seeding, 5 parts by weight of the chitosan degradation product obtained in (1) above
Solution containing 300 parts of pure chitosan and 5 parts by weight of pure product
Double-diluted solution and turfgrass obtained in (4) above diluted 300-fold
Fertilizers (B) and (C) were applied to the leaves 4 times a week (scattering).
Cloth amount 2L / m ²). 1 week after the final application (36 days of cultivation period)
In an artificial weather device (12-hour photoperiod, 25 ° C (20 during dark treatment)
C))). Then adjust the plant height and longest root length.
I checked. In the control area, tap water was sprayed.

Results: As shown in Table 2, there was no difference in plant height, but in terms of root length, the root length was clearly promoted in the chitosan degradation product-containing solution and the turfgrass growth activator sprayed area. . The promotion of root length was especially remarkable in the turfgrass growth activator sprayed area. No phytotoxicity was observed in any of the plots.

[0019]

[Table 2]

Example 3 (1) Preparation of Decomposed Product of Chitosan 5.0N of 0.2N hydrochloric acid was mixed with 5.0 of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.).
The mixture was dissolved by mixing so as to have a weight percentage, and this was used as a chitosan solution, and heated and heated at 100 ° C. for 120 minutes for hydrolysis. Then, the pH was adjusted to 5.0 with sodium hydroxide to obtain a chitosan decomposition product (B). (2) Preparation of brown juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at 130 ° C is fed into the juice to coagulate the protein, followed by centrifugation for the purpose. I got brown juice. (3) Preparation of lawn grass growth activator of the present invention 3 parts by weight of the chitosan degradation product (C) obtained in (1) above, acetic acid concentration
1 part by weight of 15% vinegar and 1 part by weight of pure water were mixed to obtain a lawn grass growth activator (D).

Further, the decomposed product of chitosan obtained in the above (1)
(C) 3 parts by weight, 1 part by weight of vinegar having an acetic acid concentration of 15% by weight, and 1 part by weight of the brown juice obtained in (2) above are mixed,
A lawn grass activator (E) was obtained. (4) Growth promotion test From the curing zone of Koshi-shiba, the Koshi-shiba was transferred to a pot (1/5000 are), and 3 parts by weight of the decomposed product of chitosan obtained in (1) above and 2 parts by weight of pure chitosan were contained. The 100-fold diluted solution and the turfgrass growth activators (D) and (E) obtained in (3) above were sprayed 5 times every 2 weeks (first spraying: October 15, 1990). Then, it sampled and investigated the dry weight of foliage and the dry weight of root. In the control area, tap water was sprayed (spraying volume ² L / m ² ).

Results: As shown in Table 3, both the dry matter weight of the foliage and the dry matter weight of the roots increased compared to the untreated plot. Especially growth activator
The increase in (D) and (E) spraying plots was remarkable. No chemical damage was observed in any of the plots during the test.

[0023]

[Table 3]

Example 4 (1) Preparation of Decomposed Product of Chitosan 5.0% by weight of chitosan (manufactured by Tokyo Chemical Industry Co., Ltd.) was added to 2.0
It was dissolved in 100 ml of a weight% lactic acid solution. Next, add 0.2 g of enzyme preparation Maceroteam 2S (manufactured by Yakult Honsha Co., Ltd.) at 45 ° C.
The reaction was carried out in a constant temperature water tank for 6 hours with slow stirring. After completion of the reaction, the enzyme was inactivated by keeping it in boiling water for 5 minutes. Then adjust to pH 5.0 with sodium hydroxide.
To obtain a decomposed product of chitosan (D). (2) Preparation of brown juice The cut alfalfa is squeezed with a squeezing machine to obtain green juice, and steam at 130 ° C is fed into the juice to coagulate the protein, followed by centrifugation for the purpose. I got brown juice. (3) Preparation of turfgrass growth activator of the present invention 3 parts by weight of the chitosan degradation product (D) obtained in (1) above, 1 part by weight of vinegar having an acetic acid concentration of 15% by weight and 1 part by weight of pure water were mixed, and turfgrass was mixed. A growth activator (F) was obtained.

The decomposed product of chitosan obtained in the above (1)
(D) 3 parts by weight, 1 part by weight of vinegar having an acetic acid concentration of 15% by weight, and 1 part by weight of the brown juice obtained in (2) above were mixed to obtain a turfgrass growth activator (G). (4) Growth promotion test In a curing area of bentgrass, a 100-fold diluted solution of a chitosan degradation product-containing solution obtained by mixing 3 parts by weight of the chitosan degradation product obtained in (1) and 2 parts by weight of pure, and (3) above A 100-fold diluted solution of the obtained lawn grass growth activators (F) and (G) was sprayed five times every two weeks (first spraying: January 30, 1990). After that, 20 samples were sampled with a hole cutter, and the longest root length was investigated. In the control area, tap water was sprayed.

Results: As shown in Table 4, the longest root length of the treated plots increased compared to the untreated plots. Especially growth activator (F),
(G) The increase in the application area was remarkable. No chemical damage was observed in any of the plots during the test.

[0027]

[Table 4]

[0028]

EFFECTS OF THE INVENTION According to the present invention, a lawn grass growth activator containing a natural material as a main component, which has no risk of adversely affecting the human body and the natural environment, and growth of turf grass having no risk of adversely affecting the human body and the natural environment. A method for activating lawn grass growth using an activator is provided.

Claims (3)

[Claims] 1. A growth activator for turfgrass, which comprises a chitosan degradation product and acetic acid as active ingredients. 2. A turfgrass growth activator comprising a chitosan degradation product, acetic acid, and alfalfa green leaf deproteinization supernatant as active ingredients. 3. A method for activating the growth of turfgrass, which comprises applying the turfgrass growth activator according to claim 1 or 2.