JPH0578314B2 - - Google Patents
Info
- Publication number
- JPH0578314B2 JPH0578314B2 JP61265090A JP26509086A JPH0578314B2 JP H0578314 B2 JPH0578314 B2 JP H0578314B2 JP 61265090 A JP61265090 A JP 61265090A JP 26509086 A JP26509086 A JP 26509086A JP H0578314 B2 JPH0578314 B2 JP H0578314B2
- Authority
- JP
- Japan
- Prior art keywords
- layer
- oxidized
- dry
- coenzyme
- detection reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001514 detection method Methods 0.000 claims description 37
- 239000003153 chemical reaction reagent Substances 0.000 claims description 34
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 29
- 239000005515 coenzyme Substances 0.000 claims description 29
- 150000001875 compounds Chemical class 0.000 claims description 28
- 229920001477 hydrophilic polymer Polymers 0.000 claims description 28
- 239000000872 buffer Substances 0.000 claims description 22
- 238000010979 pH adjustment Methods 0.000 claims description 18
- 238000004458 analytical method Methods 0.000 claims description 16
- 229960003966 nicotinamide Drugs 0.000 claims description 14
- 235000005152 nicotinamide Nutrition 0.000 claims description 14
- 239000011570 nicotinamide Substances 0.000 claims description 14
- 101710088194 Dehydrogenase Proteins 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 230000027756 respiratory electron transport chain Effects 0.000 claims description 11
- 238000011161 development Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 7
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 5
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- 238000003892 spreading Methods 0.000 description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 19
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- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 9
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- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- FBWNMEQMRUMQSO-UHFFFAOYSA-N tergitol NP-9 Chemical compound CCCCCCCCCC1=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCO)C=C1 FBWNMEQMRUMQSO-UHFFFAOYSA-N 0.000 description 3
- JEOLGCWRLRXABW-UHFFFAOYSA-N 2,5-diphenyl-2h-tetrazol-2-ium;chloride Chemical compound [Cl-].C1=CC=CC=C1C1=[NH+]N(C=2C=CC=CC=2)N=N1 JEOLGCWRLRXABW-UHFFFAOYSA-N 0.000 description 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- MUVQIIBPDFTEKM-UHFFFAOYSA-N 2-aminobutane-1,3-diol Chemical compound CC(O)C(N)CO MUVQIIBPDFTEKM-UHFFFAOYSA-N 0.000 description 2
- 108010029731 6-phosphogluconolactonase Proteins 0.000 description 2
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 2
- 108010082126 Alanine transaminase Proteins 0.000 description 2
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 2
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 2
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 description 2
- 108020005199 Dehydrogenases Proteins 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 description 2
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 description 2
- 102000057621 Glycerol kinases Human genes 0.000 description 2
- 108700016170 Glycerol kinases Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical group NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 2
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- 239000002202 Polyethylene glycol Substances 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- 239000002280 amphoteric surfactant Substances 0.000 description 2
- 150000001540 azides Chemical class 0.000 description 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 2
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- 235000011187 glycerol Nutrition 0.000 description 2
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- 239000002904 solvent Substances 0.000 description 2
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- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
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- RPZANUYHRMRTTE-UHFFFAOYSA-N 2,3,4-trimethoxy-6-(methoxymethyl)-5-[3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxyoxane;1-[[3,4,5-tris(2-hydroxybutoxy)-6-[4,5,6-tris(2-hydroxybutoxy)-2-(2-hydroxybutoxymethyl)oxan-3-yl]oxyoxan-2-yl]methoxy]butan-2-ol Chemical compound COC1C(OC)C(OC)C(COC)OC1OC1C(OC)C(OC)C(OC)OC1COC.CCC(O)COC1C(OCC(O)CC)C(OCC(O)CC)C(COCC(O)CC)OC1OC1C(OCC(O)CC)C(OCC(O)CC)C(OCC(O)CC)OC1COCC(O)CC RPZANUYHRMRTTE-UHFFFAOYSA-N 0.000 description 1
- YZIXDSVRVOZUAZ-UHFFFAOYSA-N 2-(1,2-dihydrotetrazol-1-ium-3-yl)-4,5-dimethyl-1,3-thiazole;bromide Chemical compound [Br-].S1C(C)=C(C)N=C1N1N=C[NH2+]N1 YZIXDSVRVOZUAZ-UHFFFAOYSA-N 0.000 description 1
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Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26509086A JPS63119694A (ja) | 1986-11-06 | 1986-11-06 | é žååè£é µçŽ å«æ也åŒåæèŠçŽ |
| DE8787114791T DE3783851T2 (de) | 1986-10-09 | 1987-10-09 | Integrales, mehrschichtiges element fuer analyse. |
| US07/107,674 US5063153A (en) | 1986-10-09 | 1987-10-09 | Integral multilayer analytical element |
| EP87114791A EP0264079B1 (en) | 1986-10-09 | 1987-10-09 | Integral multilayer analytical element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP26509086A JPS63119694A (ja) | 1986-11-06 | 1986-11-06 | é žååè£é µçŽ å«æ也åŒåæèŠçŽ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63119694A JPS63119694A (ja) | 1988-05-24 |
| JPH0578314B2 true JPH0578314B2 (ja) | 1993-10-28 |
Family
ID=17412458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP26509086A Granted JPS63119694A (ja) | 1986-10-09 | 1986-11-06 | é žååè£é µçŽ å«æ也åŒåæèŠçŽ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63119694A (ja) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2614124B2 (ja) * | 1989-12-08 | 1997-05-28 | å¯å£«åçãã€ã«ã æ ªåŒäŒç€Ÿ | äžäœåå€å±€åæèŠçŽ |
| JP5812672B2 (ja) * | 2011-04-28 | 2015-11-17 | ã¢ãŒã¯ã¬ã€æ ªåŒäŒç€Ÿ | ä¹³é žè±æ°ŽçŽ é µçŽ æž¬å®çšè©Šéšç |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5991896A (ja) * | 1982-11-18 | 1984-05-26 | Konishiroku Photo Ind Co Ltd | éå åè£é µçŽ æ€åºçšå€å±€åæçŽ å |
| JPS6043400A (ja) * | 1983-07-25 | 1985-03-07 | ã€âã¹ããã³ãã³ããã¯ãã«ã³ããâ | ã¢ã«ã«ãªãã¹ãã¢ã¿âãŒåæçšä¹ŸåŒåæèŠçŽ |
-
1986
- 1986-11-06 JP JP26509086A patent/JPS63119694A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5991896A (ja) * | 1982-11-18 | 1984-05-26 | Konishiroku Photo Ind Co Ltd | éå åè£é µçŽ æ€åºçšå€å±€åæçŽ å |
| JPS6043400A (ja) * | 1983-07-25 | 1985-03-07 | ã€âã¹ããã³ãã³ããã¯ãã«ã³ããâ | ã¢ã«ã«ãªãã¹ãã¢ã¿âãŒåæçšä¹ŸåŒåæèŠçŽ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63119694A (ja) | 1988-05-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |