JPH0577450B2 - - Google Patents
Info
- Publication number
- JPH0577450B2 JPH0577450B2 JP1277039A JP27703989A JPH0577450B2 JP H0577450 B2 JPH0577450 B2 JP H0577450B2 JP 1277039 A JP1277039 A JP 1277039A JP 27703989 A JP27703989 A JP 27703989A JP H0577450 B2 JPH0577450 B2 JP H0577450B2
- Authority
- JP
- Japan
- Prior art keywords
- hyaluronic acid
- acid derivative
- group
- emulsion
- product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical class CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 83
- 239000000839 emulsion Substances 0.000 claims description 34
- 125000002252 acyl group Chemical group 0.000 claims description 9
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 6
- 239000003381 stabilizer Substances 0.000 claims description 5
- 229910052783 alkali metal Inorganic materials 0.000 claims description 2
- 150000001340 alkali metals Chemical group 0.000 claims description 2
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 31
- 239000000047 product Substances 0.000 description 26
- 229920002674 hyaluronan Polymers 0.000 description 23
- 229960003160 hyaluronic acid Drugs 0.000 description 22
- 239000002502 liposome Substances 0.000 description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 18
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 18
- 238000000034 method Methods 0.000 description 18
- 239000004094 surface-active agent Substances 0.000 description 17
- -1 trehalose lipid Chemical class 0.000 description 16
- 239000000203 mixture Substances 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 14
- 235000014113 dietary fatty acids Nutrition 0.000 description 12
- 239000000194 fatty acid Substances 0.000 description 12
- 229930195729 fatty acid Natural products 0.000 description 12
- 238000002835 absorbance Methods 0.000 description 11
- 239000007795 chemical reaction product Substances 0.000 description 11
- 238000003756 stirring Methods 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 238000010438 heat treatment Methods 0.000 description 10
- 238000002834 transmittance Methods 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 229910052742 iron Inorganic materials 0.000 description 9
- 238000004945 emulsification Methods 0.000 description 8
- 229930014626 natural product Natural products 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- DEGAKNSWVGKMLS-UHFFFAOYSA-N calcein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(CN(CC(O)=O)CC(O)=O)=C(O)C=C1OC1=C2C=C(CN(CC(O)=O)CC(=O)O)C(O)=C1 DEGAKNSWVGKMLS-UHFFFAOYSA-N 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 229960002378 oftasceine Drugs 0.000 description 7
- 239000008213 purified water Substances 0.000 description 7
- 239000003549 soybean oil Substances 0.000 description 7
- 235000012424 soybean oil Nutrition 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 239000003876 biosurfactant Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 150000002148 esters Chemical class 0.000 description 6
- 238000005063 solubilization Methods 0.000 description 6
- 230000007928 solubilization Effects 0.000 description 6
- 230000006641 stabilisation Effects 0.000 description 6
- 238000011105 stabilization Methods 0.000 description 6
- 230000000087 stabilizing effect Effects 0.000 description 6
- 238000005160 1H NMR spectroscopy Methods 0.000 description 5
- 125000003047 N-acetyl group Chemical group 0.000 description 5
- 102000011420 Phospholipase D Human genes 0.000 description 5
- 108090000553 Phospholipase D Proteins 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000004530 micro-emulsion Substances 0.000 description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 5
- 229920000053 polysorbate 80 Polymers 0.000 description 5
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 4
- 102000004882 Lipase Human genes 0.000 description 4
- 108090001060 Lipase Proteins 0.000 description 4
- 239000004367 Lipase Substances 0.000 description 4
- 229920002385 Sodium hyaluronate Polymers 0.000 description 4
- 150000008065 acid anhydrides Chemical class 0.000 description 4
- 239000000654 additive Substances 0.000 description 4
- 229910001854 alkali hydroxide Inorganic materials 0.000 description 4
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 4
- 235000011114 ammonium hydroxide Nutrition 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 230000001804 emulsifying effect Effects 0.000 description 4
- 239000000787 lecithin Substances 0.000 description 4
- 235000010445 lecithin Nutrition 0.000 description 4
- 229940067606 lecithin Drugs 0.000 description 4
- 235000019421 lipase Nutrition 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- 229940010747 sodium hyaluronate Drugs 0.000 description 4
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 4
- 229930186217 Glycolipid Natural products 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000005481 NMR spectroscopy Methods 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 229920001222 biopolymer Polymers 0.000 description 3
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000008034 disappearance Effects 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000002198 insoluble material Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 235000019198 oils Nutrition 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- JQWAHKMIYCERGA-UHFFFAOYSA-N (2-nonanoyloxy-3-octadeca-9,12-dienoyloxypropoxy)-[2-(trimethylazaniumyl)ethyl]phosphinate Chemical compound CCCCCCCCC(=O)OC(COP([O-])(=O)CC[N+](C)(C)C)COC(=O)CCCCCCCC=CCC=CCCCCC JQWAHKMIYCERGA-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N Heavy water Chemical compound [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 2
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 125000004018 acid anhydride group Chemical group 0.000 description 2
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical group O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000004359 castor oil Substances 0.000 description 2
- 235000019438 castor oil Nutrition 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- ARBOVOVUTSQWSS-UHFFFAOYSA-N hexadecanoyl chloride Chemical compound CCCCCCCCCCCCCCCC(Cl)=O ARBOVOVUTSQWSS-UHFFFAOYSA-N 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002525 ultrasonication Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- RZRNAYUHWVFMIP-KTKRTIGZSA-N 1-oleoylglycerol Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(O)CO RZRNAYUHWVFMIP-KTKRTIGZSA-N 0.000 description 1
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- APLNAFMUEHKRLM-UHFFFAOYSA-N 2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]-1-(3,4,6,7-tetrahydroimidazo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1=NN=C(O1)CC(=O)N1CC2=C(CC1)N=CN2 APLNAFMUEHKRLM-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- NONFLFDSOSZQHR-UHFFFAOYSA-N 3-(trimethylsilyl)propionic acid Chemical compound C[Si](C)(C)CCC(O)=O NONFLFDSOSZQHR-UHFFFAOYSA-N 0.000 description 1
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- AFWTZXXDGQBIKW-UHFFFAOYSA-N C14 surfactin Natural products CCCCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 AFWTZXXDGQBIKW-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical class NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical group O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- MSWZFWKMSRAUBD-QZABAPFNSA-N beta-D-glucosamine Chemical group N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-QZABAPFNSA-N 0.000 description 1
- 239000003012 bilayer membrane Substances 0.000 description 1
- 239000003613 bile acid Substances 0.000 description 1
- 125000004063 butyryl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- RZRNAYUHWVFMIP-HXUWFJFHSA-N glycerol monolinoleate Natural products CCCCCCCCC=CCCCCCCCC(=O)OC[C@H](O)CO RZRNAYUHWVFMIP-HXUWFJFHSA-N 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000004820 halides Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- LHOWRPZTCLUDOI-UHFFFAOYSA-K iron(3+);triperchlorate Chemical compound [Fe+3].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O LHOWRPZTCLUDOI-UHFFFAOYSA-K 0.000 description 1
- GJMIMISVJLOUGG-UHFFFAOYSA-K iron(3+);triperchlorate;hexahydrate Chemical compound O.O.O.O.O.O.[Fe+3].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O GJMIMISVJLOUGG-UHFFFAOYSA-K 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 229940074096 monoolein Drugs 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 150000007519 polyprotic acids Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- RPACBEVZENYWOL-XFULWGLBSA-M sodium;(2r)-2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate Chemical compound [Na+].C=1C=C(Cl)C=CC=1OCCCCCC[C@]1(C(=O)[O-])CO1 RPACBEVZENYWOL-XFULWGLBSA-M 0.000 description 1
- 239000004045 soybean oil emulsion Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 description 1
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 description 1
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
Description
ïŒæè¡åéïŒ
æ¬çºæã¯ããã¢ã«ãã³é
žèªå°äœãããªãæ°èŠãª
ä¹³åå®å®å€ã«é¢ãããã®ã§ããã ïŒåŸæ¥æè¡åã³ãã®åé¡ç¹ïŒ é·éã¢ã«ãã«åºãæããå€å¡©åºé žãç³è質ãªã©
ã®åŸ®çç©ç±æ¥çé¢æŽ»æ§å€ã®æ§èœããæè¿ã泚ç®ã
ããŠããããããã¯ããããŸã§ã«ãããã€ãç¥ã
ããŠãã倩ç¶ç©ç³»ã®çé¢æŽ»æ§ç©è³ªãšãšãã«ãã€ãª
ãµãŒãã¢ã¯ã¿ã³ããšç§°ããããšãã§ãããããã
ãã€ãªãµãŒãã¢ã¯ã¿ã³ãã®çš®é¡ãšããŠã¯ãç³è質
ç³»ãšããŠããã¬ãããŒã¹ãªããããã©ã ããªãã
ãããœãããªãããããªãããçïŒèèªé žç³»ãš
ããŠãã³ãªããã³ãŒã«é žãã¹ãã¯ãªã¹ããŒã«é žã
ã¢ã¬ãªãã³é žçïŒã¢ããé žãããããç³»ãšããŠã
âã¢ã·ã«ã¢ããé žããµãŒãã¢ã¯ãã³ããªã«ãã
ã³ãªãããçïŒãã€ãªããªããŒç³»ãšããŠããšãã«
ã¶ã³ããªãããªãµãã«ã©ã€ãçïŒãã®ä»ãšããŠã
ãªã³è質ãèæ±é žããµããã³çãç¥ãããŠããã
ãããã¯ãåæ£ãä¹³åãå¯æº¶åã湿最ã浞éãæŽ
æµãªã©ã®æ¬æ¥ã®çé¢æŽ»æ§ã«å ããŠãçç掻æ§ãè¬
ç掻æ§ãéå±ã€ãªã³ææäœçšãå¢ç²ãé žåéå ã
é²éã殺èãªã©ã®æçšãªäœçšãçºæ®ãããå¯èœæ§
ãé«ããã®ã§ãããããããããããã€ãªãµãŒã
ã¢ã¯ã¿ã³ãã®çäœã«ãããå«æéãçäœããã®å
éããããŠãçç£ã³ã¹ãã«æªã æªè§£æ±ºãªåé¡ãæ®
ãããŠãããååŠè¬åãšããŠæ¯èŒç倧éãã€å®äŸ¡
ã«äŸçµŠããããŸã§ã«ã¯è³ã€ãŠããªãã®ãçŸå®ã§ã
ãã äžæ¹ã§ã¯ã倩ç¶ç©ããã®èªå°äœãšããŠãã¢ã·ã«
åãããããé žçŽ ä¿®é£ŸãŒã©ãã³ãã¢ã«ãã«ã°ãªã³
ã·ããã©ããªã³ãããžã³ã®èªå°äœãã¢ã¹ã³ã«ãã³
é žã®èèªé žãšã¹ãã«ããããŠãã·ãšç³èèªé žãšã¹
ãã«çã®æ¯èŒçæ±çšåã®èšããçé¢æŽ»æ§ç©è³ªãé
çºãããŠãããããã倩ç¶ç©ããã®èªå°äœã¯ã倧
éãã€å®äŸ¡ã«åŸãããã倩ç¶ç©ãææãšããçäœ
ç±æ¥çé¢æŽ»æ§ç©è³ªã®æš¡å£ç©ãšãããããã倧éã
ã€å®äŸ¡ã«åŸãããã倩ç¶ç©ã¯ãæè¿ã®ãã€ãªãã¯
ãããžãŒã®èããé²æ©ãšãšãã«å¢å ããŠããŠã
ããçŸåšãçäœæåã®æš¡å£ãšããŠæ§ç¯ããã€ã€ã
ã倩ç¶ç©ããã®èªå°äœã¯ãäžè¿°ããæ§ã«ãã¢ãã
é žããããããç³é¡ãææãšãããã®ãå€ããã
æè¿ç¹ã«ãå·¥æ¥çã«æ¥µããŠçŽåºŠã®é«ãå€ç³äœãåŸ
ãããšãå¯èœãšãªãããã®å©çšãèå³ãããã®ãš
ãããŠããã å€ç³äœã«çé¢æŽ»æ§å€ãšããŠã®ç¹æ§ã®åŽé¢ãæã
ããããšã¯ãäžè¿°ã®ãã€ãªããªããŒç³»ãã€ãªãµãŒ
ãã¢ã¯ã¿ã³ãã§ãããšãã«ã¶ã³ããªãããªãµãã«
ã©ã€ãã人çºçã«äœãåºãããšã§ãããäžã€ãçš
ããå€ç³äœãéžæããããšã«ãããçäœç±æ¥çé¢
掻æ§å€ãããããã«å€ãã®ç¹æ§ãæãããã®ãåµ
補ãåŸãå¯èœæ§ãããã ãŸããè¿å¹Žãä¹³åã«é¢ããæ°å€ãã®ç 究éçºã
ãªãããæ°èŠã®ä¹³åå€åã³ä¹³åæè¡ã®é²æ©ã¯èã
ããå®å®æ§ã®éåžžã«è¯ãä¹³åçµæç©ãå€çš®ç£æ¥å
éã§åºãå©çšãããããã«ãªã€ãããããããã®
å€ãã¯åæãããçé¢æŽ»æ§å€ïŒéã€ãªã³æ§ãéœã€
ãªã³æ§ãé°ã€ãªã³æ§åã¯äž¡æ§ã®ãã®ïŒã䜿çšãã
ãã®ã§ãããäžè¬æ¶è²»è éã§ã¯å®å šã®é¢ã§ã®å€ã
ã®äžå®æãããããŠããããããŠãæè¿ã®å€©ç¶ã
èªç¶ãžã®å¿åãåæ ãã倩ç¶ç©ãããã¯å€©ç¶ç©ã
ãèªå°ããããããã倩ç¶ç³»çé¢æŽ»æ§å€ãçšãã
ããããã«ãªã€ãŠãããããããå®å šå€©ç¶ç©ãšã
ãŠã¯ãã¬ã·ãã³ããããå©çšãããŠããã ãã§ã
ã²ããå©çšãããŠæ¥ã€ã€ããã·ãšç³èèªé žãšã¹ã
ã«åã³ã¢ããé žç³»çé¢æŽ»æ§å€ã¯ã倩ç¶ç©æ§æèŠçŽ
ãææã«ããŠãããšããããšã§ããããããã·ãš
ç³èèªé žãšã¹ãã«åã³ã¢ããé žç³»çé¢æŽ»æ§å€ã¯ã
äžè¿°ã®ãã€ãªãµãŒãã¢ã¯ã¿ã³ãã®çš®é¡ãšããŠã¯ã
ç³è質系åã³ã¢ããé žç³»ã®æš¡å£ç©ãšãããããšã
ããããã€ãªããªããŒç³»ã«ããã€ãŠã¯ãæåãªå€©
ç¶æ§æèŠçŽ ãååŠä¿®é£Ÿãããããªæ§é ã®çé¢æŽ»æ§
å€ã¯åŸãããŠããªãããã®ãããªé«ååç³»çé¢æŽ»
æ§å€ã¯ãäžè¬çã«æ°Žæº¶æ§é«ååã®æããåæ£å®å®
åãç²åºŠèª¿æŽäœçšçãæåŸ ã§ããåŸãããä¹³åçµ
æç©ã«å¹ åºãçšéãæãããããšãã§ããã ïŒçºæã®èª²é¡ïŒ æ¬çºæã¯ãçäœç±æ¥ã®å®å šæ§ã®é«ãæ°èŠãªä¹³å
å®å®å€ãæäŸããããšããã®èª²é¡ãšããã ïŒèª²é¡ã解決ããããã®ææ®µïŒ æ¬çºæè ãã¯ãåèšèª²é¡ã解決ãã¹ãéæç 究
ãéããçµæãç¹å®ã®ãã¢ã«ãã³é žèªå°äœããã
ããä¹³åå®å®åäœçšãæããããšãèŠåºããæ¬çº
æãå®æããã«è³ã€ãã ããªãã¡ãæ¬çºæã«ããã°ãäžèšäžè¬åŒïŒïŒ
åã³äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°
äœã®äžããéžã°ããä¹³åå®å®å€ãæäŸãããã
ä¹³åå®å®å€ã«é¢ãããã®ã§ããã ïŒåŸæ¥æè¡åã³ãã®åé¡ç¹ïŒ é·éã¢ã«ãã«åºãæããå€å¡©åºé žãç³è質ãªã©
ã®åŸ®çç©ç±æ¥çé¢æŽ»æ§å€ã®æ§èœããæè¿ã泚ç®ã
ããŠããããããã¯ããããŸã§ã«ãããã€ãç¥ã
ããŠãã倩ç¶ç©ç³»ã®çé¢æŽ»æ§ç©è³ªãšãšãã«ãã€ãª
ãµãŒãã¢ã¯ã¿ã³ããšç§°ããããšãã§ãããããã
ãã€ãªãµãŒãã¢ã¯ã¿ã³ãã®çš®é¡ãšããŠã¯ãç³è質
ç³»ãšããŠããã¬ãããŒã¹ãªããããã©ã ããªãã
ãããœãããªãããããªãããçïŒèèªé žç³»ãš
ããŠãã³ãªããã³ãŒã«é žãã¹ãã¯ãªã¹ããŒã«é žã
ã¢ã¬ãªãã³é žçïŒã¢ããé žãããããç³»ãšããŠã
âã¢ã·ã«ã¢ããé žããµãŒãã¢ã¯ãã³ããªã«ãã
ã³ãªãããçïŒãã€ãªããªããŒç³»ãšããŠããšãã«
ã¶ã³ããªãããªãµãã«ã©ã€ãçïŒãã®ä»ãšããŠã
ãªã³è質ãèæ±é žããµããã³çãç¥ãããŠããã
ãããã¯ãåæ£ãä¹³åãå¯æº¶åã湿最ã浞éãæŽ
æµãªã©ã®æ¬æ¥ã®çé¢æŽ»æ§ã«å ããŠãçç掻æ§ãè¬
ç掻æ§ãéå±ã€ãªã³ææäœçšãå¢ç²ãé žåéå ã
é²éã殺èãªã©ã®æçšãªäœçšãçºæ®ãããå¯èœæ§
ãé«ããã®ã§ãããããããããããã€ãªãµãŒã
ã¢ã¯ã¿ã³ãã®çäœã«ãããå«æéãçäœããã®å
éããããŠãçç£ã³ã¹ãã«æªã æªè§£æ±ºãªåé¡ãæ®
ãããŠãããååŠè¬åãšããŠæ¯èŒç倧éãã€å®äŸ¡
ã«äŸçµŠããããŸã§ã«ã¯è³ã€ãŠããªãã®ãçŸå®ã§ã
ãã äžæ¹ã§ã¯ã倩ç¶ç©ããã®èªå°äœãšããŠãã¢ã·ã«
åãããããé žçŽ ä¿®é£ŸãŒã©ãã³ãã¢ã«ãã«ã°ãªã³
ã·ããã©ããªã³ãããžã³ã®èªå°äœãã¢ã¹ã³ã«ãã³
é žã®èèªé žãšã¹ãã«ããããŠãã·ãšç³èèªé žãšã¹
ãã«çã®æ¯èŒçæ±çšåã®èšããçé¢æŽ»æ§ç©è³ªãé
çºãããŠãããããã倩ç¶ç©ããã®èªå°äœã¯ã倧
éãã€å®äŸ¡ã«åŸãããã倩ç¶ç©ãææãšããçäœ
ç±æ¥çé¢æŽ»æ§ç©è³ªã®æš¡å£ç©ãšãããããã倧éã
ã€å®äŸ¡ã«åŸãããã倩ç¶ç©ã¯ãæè¿ã®ãã€ãªãã¯
ãããžãŒã®èããé²æ©ãšãšãã«å¢å ããŠããŠã
ããçŸåšãçäœæåã®æš¡å£ãšããŠæ§ç¯ããã€ã€ã
ã倩ç¶ç©ããã®èªå°äœã¯ãäžè¿°ããæ§ã«ãã¢ãã
é žããããããç³é¡ãææãšãããã®ãå€ããã
æè¿ç¹ã«ãå·¥æ¥çã«æ¥µããŠçŽåºŠã®é«ãå€ç³äœãåŸ
ãããšãå¯èœãšãªãããã®å©çšãèå³ãããã®ãš
ãããŠããã å€ç³äœã«çé¢æŽ»æ§å€ãšããŠã®ç¹æ§ã®åŽé¢ãæã
ããããšã¯ãäžè¿°ã®ãã€ãªããªããŒç³»ãã€ãªãµãŒ
ãã¢ã¯ã¿ã³ãã§ãããšãã«ã¶ã³ããªãããªãµãã«
ã©ã€ãã人çºçã«äœãåºãããšã§ãããäžã€ãçš
ããå€ç³äœãéžæããããšã«ãããçäœç±æ¥çé¢
掻æ§å€ãããããã«å€ãã®ç¹æ§ãæãããã®ãåµ
補ãåŸãå¯èœæ§ãããã ãŸããè¿å¹Žãä¹³åã«é¢ããæ°å€ãã®ç 究éçºã
ãªãããæ°èŠã®ä¹³åå€åã³ä¹³åæè¡ã®é²æ©ã¯èã
ããå®å®æ§ã®éåžžã«è¯ãä¹³åçµæç©ãå€çš®ç£æ¥å
éã§åºãå©çšãããããã«ãªã€ãããããããã®
å€ãã¯åæãããçé¢æŽ»æ§å€ïŒéã€ãªã³æ§ãéœã€
ãªã³æ§ãé°ã€ãªã³æ§åã¯äž¡æ§ã®ãã®ïŒã䜿çšãã
ãã®ã§ãããäžè¬æ¶è²»è éã§ã¯å®å šã®é¢ã§ã®å€ã
ã®äžå®æãããããŠããããããŠãæè¿ã®å€©ç¶ã
èªç¶ãžã®å¿åãåæ ãã倩ç¶ç©ãããã¯å€©ç¶ç©ã
ãèªå°ããããããã倩ç¶ç³»çé¢æŽ»æ§å€ãçšãã
ããããã«ãªã€ãŠãããããããå®å šå€©ç¶ç©ãšã
ãŠã¯ãã¬ã·ãã³ããããå©çšãããŠããã ãã§ã
ã²ããå©çšãããŠæ¥ã€ã€ããã·ãšç³èèªé žãšã¹ã
ã«åã³ã¢ããé žç³»çé¢æŽ»æ§å€ã¯ã倩ç¶ç©æ§æèŠçŽ
ãææã«ããŠãããšããããšã§ããããããã·ãš
ç³èèªé žãšã¹ãã«åã³ã¢ããé žç³»çé¢æŽ»æ§å€ã¯ã
äžè¿°ã®ãã€ãªãµãŒãã¢ã¯ã¿ã³ãã®çš®é¡ãšããŠã¯ã
ç³è質系åã³ã¢ããé žç³»ã®æš¡å£ç©ãšãããããšã
ããããã€ãªããªããŒç³»ã«ããã€ãŠã¯ãæåãªå€©
ç¶æ§æèŠçŽ ãååŠä¿®é£Ÿãããããªæ§é ã®çé¢æŽ»æ§
å€ã¯åŸãããŠããªãããã®ãããªé«ååç³»çé¢æŽ»
æ§å€ã¯ãäžè¬çã«æ°Žæº¶æ§é«ååã®æããåæ£å®å®
åãç²åºŠèª¿æŽäœçšçãæåŸ ã§ããåŸãããä¹³åçµ
æç©ã«å¹ åºãçšéãæãããããšãã§ããã ïŒçºæã®èª²é¡ïŒ æ¬çºæã¯ãçäœç±æ¥ã®å®å šæ§ã®é«ãæ°èŠãªä¹³å
å®å®å€ãæäŸããããšããã®èª²é¡ãšããã ïŒèª²é¡ã解決ããããã®ææ®µïŒ æ¬çºæè ãã¯ãåèšèª²é¡ã解決ãã¹ãéæç 究
ãéããçµæãç¹å®ã®ãã¢ã«ãã³é žèªå°äœããã
ããä¹³åå®å®åäœçšãæããããšãèŠåºããæ¬çº
æãå®æããã«è³ã€ãã ããªãã¡ãæ¬çºæã«ããã°ãäžèšäžè¬åŒïŒïŒ
åã³äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°
äœã®äžããéžã°ããä¹³åå®å®å€ãæäŸãããã
ãåã
ãåã
ïŒåèšåŒäžãR1ã¯ã¢ã·ã«åºãæå³ããR2ã¯ã
ãããã·ã«åºãâOMåºïŒåŒäžãïŒã¯ã¢ã«ã«ãªé
å±ååãæå³ããïŒåã¯âNH2åºãæå³ããã
ããŠïœã¯ïŒä»¥äžã®æŽæ°ãæå³ããïŒ åèšR1ã§è¡šããããã¢ã·ã«åºã®ççŽ ååæ°ã¯ã
ïŒã30ã奜ãŸããã¯ïŒã20ã§ããããã®ãããªã¢
ã·ã«åºãšããŠã¯ãã¢ã»ãã«åºãããããªãã«åºã
ãããªã«åºãããã·ãã€ã«åºçã®äœçŽã¢ã·ã«åºã®
ä»ãåçš®é«çŽã¢ã·ã«åºãå å«ããããé«çŽã¢ã·ã«
åºã¯ã飜ååã¯äžé£œåé«çŽèèªé žãäŸãã°ãã©ãŠ
ãªã³é žãããªã¹ãã³é žããã«ããã³é žãã¹ãã¢ãª
ã³é žããªã¬ã€ã³é žããªãã€ã³é žçããèªå°ããã
ãšãã§ããã æ¬çºæã«ããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«
ãã³é žèªå°äœã¯ããã¢ã«ãã³é žåã¯ãã®å¡©ãèèª
é žåã¯ãã®ãã©ã€ããšåå¿ãããããšã«ãã€ãŠåŸ
ãããšãã§ããããã®åå¿ïŒã¢ã·ã«ååå¿ïŒã«ã
ããŠã¯ããã®èèªé žã®ã«ã«ããã·ã«åºããã¢ã«ã
ã³é žã®ïŒ€âã°ã«ã¯ãã³é žéšåã®æ°Žé žåºåã³ïŒ€âã°
ã«ã³ãµãã³éšåã«çµåããããããã·ã¡ãã«åºã®
æ°Žé žåºãšåå¿ããŠããã¢ã«ãã³é žã®ååå ã«ã¢ã·
ã«åºãå°å ¥ãããããã¢ã«ãã³é žã®ååéã¯ïŒäž
ã200äžã§ããããäžè¬çä¹³åå®å®å€ãšããŠã¯ã
ååéïŒäžã10äžã®ãã¢ã«ãã³é žã®äœ¿çšã奜ãŸã
ãã ãŸããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é ž
èªå°äœã«ãããŠãR2ãâNH2åºã§ãããã®ã¯ã
äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°äœã
ã¢ã³ã¢ãã¢æ°Žã§åŠçããããšã«ãã€ãŠåŸãããšã
ã§ãããããã«ãäžè¬åŒïŒïŒã§è¡šãããããã¢
ã«ãã³é žèªå°äœã«ãããŠãR2ãããããã·åºã§
ãããã®ã¯ãäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ã
ã³é žèªå°äœãæ°Žé žåã¢ã«ã«ãªæ°Žæº¶æ¶²ã§åŠçããã
ãšã«ãã€ãŠãåŸãããšãã§ããã äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°äœ
ãåŸãããã®æ¹æ³ãããå ·äœçã«ç€ºããšãäŸã
ã°ãåºçºç©è³ªã®ãã¢ã«ãã³é žãç¡æ°Žãžã¡ãã«ãã«
ã ã¢ãããªã©ã®æº¶æ¶²äžã«äŸãã°50âãªãã60âã®
å æž©äžã§åæ£ãããã次ã«ç¡æ°Žããªãžã³ãšç¡æ°Žãž
ã¡ãã«ãã«ã ã¢ãããªã©ã®æº¶åªã«èèªé žãã©ã€ã
ãããããã溶解ãããã®ãå ãã50ã60âã§æ°
æéåŒç¶ã宀枩ã§æ°æéãªããæ°æ¥éæ¹æããªã
ãåå¿ããããåŸãããåå¿çæç©ã¯ãããåžžæ³
ã«ããæŽæµã粟補ããããšã«ãããäžè¬åŒïŒïŒ
ã§è¡šãããããã¢ã«ãã³é žèªå°äœã§åŸããããäž
è¬åŒïŒïŒã«ãããŠãR2ãâOMåºã§ãããã®
ã¯ãäžè¬åŒïŒïŒã«ãããŠR2ãâOHåºã§ããã
ã®ãæ°Žé žåã¢ã«ã«ãªãšåå¿ãããããšã«ãã€ãŠåŸ
ãããšãã§ããã åè¿°ããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³
é žèªå°äœã¯ããããå æ°Žå解åŠçããããšã«ã
ããã°ã«ã¯ãã³é žéšã«ãšã¹ãã«çµåããŠããèèª
é žç¡æ°Žç©ãè±é¢ããŠéé¢ã«ã«ãã³é žã«å€æããã
ãã¢ã«ãã³é žèªå°äœãšããããšãã§ãããããå ·
äœçã«ã¯ãããããšã¹ãã«çµåã«ããé žç¡æ°Žç©ã
ã¯ãããã«ã ã«åæ£ããæ°Žé žåãããªãŠã ãªã©ã®
æ°Žé žåã¢ã«ã«ãªãå°éå ããŠå®€æž©ã§æ°æéåå¿ã
ããããšã«ããéé¢ã«ã«ãã³é žã®åœ¢æ ãæããã
ã¢ã«ãã³é žèªå°äœãåŸãããšãã§ããããŸããã
ã®å Žåãæ°Žé žåã¢ã«ã«ãªã«ä»£ããŠã¢ã³ã¢ãã¢æ°Žã
çšããããšã«ãããã«ã«ãã³é žã¢ããã®åœ¢æ ãæ
ããèªå°äœãåŸãããšãã§ããããã®ããã«ããŠ
åŸãããçæç©ãåžžæ³ã«ãã€ãŠç²Ÿè£œããããšã«ã
ãææã®ãã¢ã«ãã³é žèªå°äœãšããããšãã§ã
ãã æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãèµ€å€ç·åž
åã¹ãã¯ãã«ïŒKBræ³ïŒãH1âNMRã¹ãã¯ãã«
ïŒæº¶åªéæ°Žãå éšæšæºTMSPïŒã«ãã€ãŠåºå®ã
ãããŸãçæç©äžã®ã¢ã·ã«åºã®å«æçã¯åå¿ã¢ã«
æ¯ãåå¿æž©åºŠåã³åå¿æéãæŽã«ã¢ã«ã«ãªåŠçæ¡
件çã«ãã€ãŠèª¿æŽããããšãã§ããã ïŒçºæã®å¹æïŒ æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãçé¢æŽ»æ§
äœçšãæããç¹ã«ãä¹³åäœçšãšåæã«ä¹³åçµæç©
å®å®åãããäœçšãæãããã®ã§ããã äžè¬ã«ãä¹³åçµæç©ã«ãããŠã¯ãä¹³åå€ãšãšã
ã«ãïŒäŸ¡ã¢ã«ã³ãŒã«ãå€äŸ¡ã¢ã«ã³ãŒã«ãå©å€ãšã
ãŠæ·»å ããŠããããæ¬çºæã®ãã¢ã«ãã³é žèªå°äœ
ã¯ããã®ååå ã«æ°Žé žåºãæããŠãããããã¢ã«
ã³ãŒã«é¡ãšã®çµåããç¹ã«èæ ®ããªããŠããå®å®
æ§ã®ããä¹³åçµæç©ãäžããããŸãçšãããã¢ã«
ãã³é žèªå°äœã®ååéãéžæããäºã«ãããä¹³å
çµæç©ã®ç²åºŠã®èª¿æŽãå¯èœã§ãããå®éã®å粧å
ã®è£œå圢æ ã«ãããŠãä¹³ååãžã®ãã¢ã«ãã³é žé
åãšãã圢ããæ¯èŒç容æã§ããäžãåæã«ä»æ
åãšã®çµã¿åããã«ãå¹ ãã§ãããšããå©ç¹ãåŸ
ãããã ããã«ãæ¬çºæã®ãã¢ã«ãã³é žèªå°äœã¯ãéåžž
ã«ããããä¹³åèœåã³ãã®å®å®åäœçšããããç¹
ã«ãåç¬ã§ã®ïŒ¯ïŒïŒ·åä¹³åç³»ãžã®æ·»å ã«ãããŠã
æ¯èŒçååéã®äœããã¢ã«ãã³é žèªå°äœã«ããè¯
奜ãªä¹³åå®å®æ§ãåŸãããããã¢ã«ãã³é žèªå°äœ
ã®ååéã倧ãããããšä¹³åç²åã®åéãèµ·ãã€
ãŠãä¹³åç³»ãäžå®å®ã«ãªãçã®åé¡ãããã®ã§ã
ã¢ã«ãã³é žèªå°äœã®äœ¿çšã«ããã€ãŠã¯ããã®åå
éããä¹³åç²åãµã€ãºåã³ä¹³åæ¹æ³ã®éžæãé©åœ
ã«è¡ãããšéèŠã§ããããããã®ããšãèæ ®ãã
ããšã«ãããå®å®æ§ã®ããä¹³åç³»ãåŸãããšãã§
ãããä¹³åç³»ã®å®å®åããã€ã¯ããšãã«ãžãšã³å
ã«ããè¡ãå Žåã«ã¯ãä¹³åç²åã®è¡šé¢ç©ã®å¢å€§ã«
ãããçé¢æŽ»æ§å€ã®äœ¿çšéã®å¢å ãå¿ èŠãšããã
ããæ¬çºæã®ãã¢ã«ãã³é žèªå°äœã«ããå®å®åã§
ã¯ããã®ãããªçé¢æŽ»æ§å€ã®äœ¿çšéã®å¢å ã¯é²æ¢
ããããããªãã¡ãç¹ã«è£ 眮çã«é倧ãªãã€ã¯ã
ãšãã«ãžãšã³åãå³ããªããšãä»»æã«éžæããã
ãã¢ã«ãã³é žèªå°äœã«ãããä¹³åç³»ã®å®å®åãå³
ãããšãã§ãããããŸãäžå®ã®ä¹³åæ¹æ³ãéžæã
ãéã«ããã¢ã«ãã³é žèªå°äœã®ååãµã€ãºã®éžæ
ã«ãããä¹³åç²åç³»ã®åäžæ§åã³ä¹³åç²ååŸãå¶
埡ããããšãå¯èœã§ããã æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãä¹³åç©ã®
äžã€ã®åœ¢æ ã§ãããªããœãŒã ã®å®å®åã«ãããã
å¹æã瀺ãããã¢ã«ãã³é žããªããœãŒã ã®è¡šé¢è¢«
èŠã®ç®çã§çšããäŸã¯ãç¹éæ59â210013å·å ¬å ±
ã«ç€ºãããŠãããããã®å Žåã¯ãã«ã«ããã·ã¡ã
ã«åãã¢ã«ãã³é žãšãªããœãŒã æ§æã¬ã·ãã³ãšã®
éé»çãªçµåãè¡ã€ãŠããã«éãããåšå²ã®PHã
å¡©æ¿åºŠã«ãããã®è¢«èŠã¯å®¹æã«è§£é¢ããŠããŸãã
æ¬çºæã®ãã¢ã«ãã³é žèªå°äœã氎溶æ§è¢å è²çŽ ã®
ã«ã«ã»ã€ã³ãå å ãããªããœãŒã ã®è¢«èŠã«çšãã
æã«ã¯ããã¹ããªããŒãŒïŒ€ã«ãã€ãŠãããããã
ã«ã«ã»ã€ã³ã®æŸåºãæå¶ãããã æ¬çºæã®ãã¢ã«ãã³é žèªå°äœã¯ãä¹³åå€åã³ä¹³
åå®å®å€ãšããŠãä¹³åçµæç©ãæ±ãåéãäŸã
ã°ãå粧åãå»è¬åãå»è¬éšå€éšããã€ã¬ã¿ãªãŒ
補åãæŽæµå€ãé£åçã®åéã«æå©ã«å©çšãã
ãã ïŒå®æœäŸïŒ æ¬çºæãå®æœäŸã«ãã€ãŠæŽã«è©³çŽ°ã«èª¬æããã åèäŸ ïŒ 500mlã®ãã¹åãã©ã¹ã³äžã§ãã¢ã«ãã³é žãã
ãªãŠã ïŒå¹³åååé2000000ïŒ0.2ïœïŒ0.2ïŒ401ïŒ
5.0Ã10-4ã¢ã«ç³åäœïŒãç¡æ°Žãžã¡ãã«ãã«ã ã¢
ãã200mlã«50ã60âã§å æž©ããªããåæ£ããã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããç¡æ°Žã
ãªãžã³1.6gïŒ2.0Ã10-2ã¢ã«ïŒåã³ãã«ããã€ã«ã¯
ãã©ã€ã4.5gïŒ2.0Ã10-2ã¢ã«ïŒãç¡æ°Žãžã¡ãã«ã
ã«ã ã¢ããã®é©éã«æº¶è§£ãããã®ãå ããããã
ã60âã§ïŒæéä¿ã¡ããã®åŸå®€æž©ã«ãŠïŒã±æéæŸ
眮ãããïŒã±æåŸãåå¿æ··åç©ãå床60âã«30å
éå æž©ããå·åŽããªããã¡ã«æžæ¿ç©ãæ¿Ÿéããã
ãã®åå¿çæç©ãã¢ã»ãã³åã³ã¯ãããã«ã ã§æŽ
æµããæžå§äžãå ç±ä¹Ÿç¥ããã 也ç¥åŸã®åå¿çæç©ã®IRåã³NMRã枬å®ã
ãããã®åå¿çæç©ã®IRåæçµæã«ãããŠã¯ã
1740cm-1ã«ã«ã«ããã«åºã®åžåãèŠãããããã«
ãããšã¹ãã«åœ¢æã®ç¢ºèªãããããŸãåæã«1810
cm-1ã«ãåžåãèªããã°ã«ã¯ãã³é žéšã®ã«ã«ãã
ã·ã«åºã«é žç¡æ°Žç©ã圢æãããŠããããšã確èªã
ãããŸãããã®åå¿çæç©ã®NMRåæçµæã«ã
ããŠã¯ã0.9ppmåã³1.28ppmã«ãã«ããã€ã«åº
ã®ãããã³ã®ããŒã¯ã確èªããã ãŸãããã®åå¿çæç©ã«ãããèèªé žãšã¹ãã«
ãååšããããšãããããããµã é žéæ³ã«ãã€ãŠ
次ã®ããã«ããŠç¢ºèªããããŸãè©ŠæãšããŠåå¿ç
æç©ïŒmgã粟秀ãããšã¿ããŒã«0.6mlã«æžæ¿ããã
ãã®æžæ¿æ¶²ã«ãå¥ã«èª¿æŽããã¢ã«ã«ãªæ§ãããã
ã·ã«ã¢ãã³æ¶²1.25mlãæ·»å ãã65âã§ïŒåéå ç±
ããã ãªããã¢ã«ã«ãªæ§ããããã·ã«ã¢ãã³æ¶²ã¯æ¬¡ã®
ããã«ããŠèª¿æŽãããå¡©é žããããã·ã«ã¢ãã³
2gã粟補氎2.5mlã«æº¶è§£ããç¡æ°Žãšã¿ããŒã«ã§50
mlãšãããã®ã液ãšããæ°Žé žåãããªãŠã 4g
ã粟補氎2.5mlã«æº¶è§£ããç¡æ°Žãšã¿ããŒã«50mlãš
ãããã®ã液ãšããã䜿çšçŽåã«ïŒ¡æ¶²ãšïŒ¢æ¶²ãš
ãçéæ··åããçããå¡©åãããªãŠã ãé å¿é€å»
ããŠäœ¿çšæ¶²ãšããã 次ã«ãåèš65âã§å ç±ããåå¿æ¶²ã宀枩ã§ïŒå
éå·åŽããåŸããã®åå¿æ¶²ã«éå¡©çŽ é žé溶液ïŒé
å¡©çŽ é žç¬¬äºéå æ°Žå¡©5gã70ïŒ éå¡©çŽ é ž10mlãšç²Ÿ
補氎10mlã«æº¶è§£ããå·ãšã¿ããŒã«ã§100mlãšãã
ãã®ãå·æä¿å液ãšãããã®ä¿å液ïŒmlãš70ïŒ é
å¡©çŽ é žïŒmlãåããå·ãšã¿ããŒã«ã§100mlãšãã
ãã®ïŒ3.15mlãæŽã«æ·»å ããŠãããæ¹æããŠ30å
åŸã«ã530nmã«ãããçºè²åžå 床ã枬å®ãããæ¯
èŒã®ããã®è©Šæãå«ãŸãªããã©ã³ã¯ã®åžå 床ã
0.072ã§ããã«ã®ã«å¯Ÿããè©ŠæïŒmgãå«æããã
ã®ã®åžå 床ã¯1.79ã§ããããšã枬å®ãããèèªé ž
ãšã¹ãã«ã®ååšã確èªãããã ãã®åå¿çæç©ã§ãããã«ããã€ã«åºå°å ¥ãã¢
ã«ãã³é žèªå°äœïŒã¯æ¬¡ã®ãããªååæ§é ãæãã
ããšã確èªãããããŸããã®ãã®ã¯æ°Žäžæº¶æ§ã®ã
ã®ã§ãã€ãã
ãããã·ã«åºãâOMåºïŒåŒäžãïŒã¯ã¢ã«ã«ãªé
å±ååãæå³ããïŒåã¯âNH2åºãæå³ããã
ããŠïœã¯ïŒä»¥äžã®æŽæ°ãæå³ããïŒ åèšR1ã§è¡šããããã¢ã·ã«åºã®ççŽ ååæ°ã¯ã
ïŒã30ã奜ãŸããã¯ïŒã20ã§ããããã®ãããªã¢
ã·ã«åºãšããŠã¯ãã¢ã»ãã«åºãããããªãã«åºã
ãããªã«åºãããã·ãã€ã«åºçã®äœçŽã¢ã·ã«åºã®
ä»ãåçš®é«çŽã¢ã·ã«åºãå å«ããããé«çŽã¢ã·ã«
åºã¯ã飜ååã¯äžé£œåé«çŽèèªé žãäŸãã°ãã©ãŠ
ãªã³é žãããªã¹ãã³é žããã«ããã³é žãã¹ãã¢ãª
ã³é žããªã¬ã€ã³é žããªãã€ã³é žçããèªå°ããã
ãšãã§ããã æ¬çºæã«ããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«
ãã³é žèªå°äœã¯ããã¢ã«ãã³é žåã¯ãã®å¡©ãèèª
é žåã¯ãã®ãã©ã€ããšåå¿ãããããšã«ãã€ãŠåŸ
ãããšãã§ããããã®åå¿ïŒã¢ã·ã«ååå¿ïŒã«ã
ããŠã¯ããã®èèªé žã®ã«ã«ããã·ã«åºããã¢ã«ã
ã³é žã®ïŒ€âã°ã«ã¯ãã³é žéšåã®æ°Žé žåºåã³ïŒ€âã°
ã«ã³ãµãã³éšåã«çµåããããããã·ã¡ãã«åºã®
æ°Žé žåºãšåå¿ããŠããã¢ã«ãã³é žã®ååå ã«ã¢ã·
ã«åºãå°å ¥ãããããã¢ã«ãã³é žã®ååéã¯ïŒäž
ã200äžã§ããããäžè¬çä¹³åå®å®å€ãšããŠã¯ã
ååéïŒäžã10äžã®ãã¢ã«ãã³é žã®äœ¿çšã奜ãŸã
ãã ãŸããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é ž
èªå°äœã«ãããŠãR2ãâNH2åºã§ãããã®ã¯ã
äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°äœã
ã¢ã³ã¢ãã¢æ°Žã§åŠçããããšã«ãã€ãŠåŸãããšã
ã§ãããããã«ãäžè¬åŒïŒïŒã§è¡šãããããã¢
ã«ãã³é žèªå°äœã«ãããŠãR2ãããããã·åºã§
ãããã®ã¯ãäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ã
ã³é žèªå°äœãæ°Žé žåã¢ã«ã«ãªæ°Žæº¶æ¶²ã§åŠçããã
ãšã«ãã€ãŠãåŸãããšãã§ããã äžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³é žèªå°äœ
ãåŸãããã®æ¹æ³ãããå ·äœçã«ç€ºããšãäŸã
ã°ãåºçºç©è³ªã®ãã¢ã«ãã³é žãç¡æ°Žãžã¡ãã«ãã«
ã ã¢ãããªã©ã®æº¶æ¶²äžã«äŸãã°50âãªãã60âã®
å æž©äžã§åæ£ãããã次ã«ç¡æ°Žããªãžã³ãšç¡æ°Žãž
ã¡ãã«ãã«ã ã¢ãããªã©ã®æº¶åªã«èèªé žãã©ã€ã
ãããããã溶解ãããã®ãå ãã50ã60âã§æ°
æéåŒç¶ã宀枩ã§æ°æéãªããæ°æ¥éæ¹æããªã
ãåå¿ããããåŸãããåå¿çæç©ã¯ãããåžžæ³
ã«ããæŽæµã粟補ããããšã«ãããäžè¬åŒïŒïŒ
ã§è¡šãããããã¢ã«ãã³é žèªå°äœã§åŸããããäž
è¬åŒïŒïŒã«ãããŠãR2ãâOMåºã§ãããã®
ã¯ãäžè¬åŒïŒïŒã«ãããŠR2ãâOHåºã§ããã
ã®ãæ°Žé žåã¢ã«ã«ãªãšåå¿ãããããšã«ãã€ãŠåŸ
ãããšãã§ããã åè¿°ããäžè¬åŒïŒïŒã§è¡šãããããã¢ã«ãã³
é žèªå°äœã¯ããããå æ°Žå解åŠçããããšã«ã
ããã°ã«ã¯ãã³é žéšã«ãšã¹ãã«çµåããŠããèèª
é žç¡æ°Žç©ãè±é¢ããŠéé¢ã«ã«ãã³é žã«å€æããã
ãã¢ã«ãã³é žèªå°äœãšããããšãã§ãããããå ·
äœçã«ã¯ãããããšã¹ãã«çµåã«ããé žç¡æ°Žç©ã
ã¯ãããã«ã ã«åæ£ããæ°Žé žåãããªãŠã ãªã©ã®
æ°Žé žåã¢ã«ã«ãªãå°éå ããŠå®€æž©ã§æ°æéåå¿ã
ããããšã«ããéé¢ã«ã«ãã³é žã®åœ¢æ ãæããã
ã¢ã«ãã³é žèªå°äœãåŸãããšãã§ããããŸããã
ã®å Žåãæ°Žé žåã¢ã«ã«ãªã«ä»£ããŠã¢ã³ã¢ãã¢æ°Žã
çšããããšã«ãããã«ã«ãã³é žã¢ããã®åœ¢æ ãæ
ããèªå°äœãåŸãããšãã§ããããã®ããã«ããŠ
åŸãããçæç©ãåžžæ³ã«ãã€ãŠç²Ÿè£œããããšã«ã
ãææã®ãã¢ã«ãã³é žèªå°äœãšããããšãã§ã
ãã æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãèµ€å€ç·åž
åã¹ãã¯ãã«ïŒKBræ³ïŒãH1âNMRã¹ãã¯ãã«
ïŒæº¶åªéæ°Žãå éšæšæºTMSPïŒã«ãã€ãŠåºå®ã
ãããŸãçæç©äžã®ã¢ã·ã«åºã®å«æçã¯åå¿ã¢ã«
æ¯ãåå¿æž©åºŠåã³åå¿æéãæŽã«ã¢ã«ã«ãªåŠçæ¡
件çã«ãã€ãŠèª¿æŽããããšãã§ããã ïŒçºæã®å¹æïŒ æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãçé¢æŽ»æ§
äœçšãæããç¹ã«ãä¹³åäœçšãšåæã«ä¹³åçµæç©
å®å®åãããäœçšãæãããã®ã§ããã äžè¬ã«ãä¹³åçµæç©ã«ãããŠã¯ãä¹³åå€ãšãšã
ã«ãïŒäŸ¡ã¢ã«ã³ãŒã«ãå€äŸ¡ã¢ã«ã³ãŒã«ãå©å€ãšã
ãŠæ·»å ããŠããããæ¬çºæã®ãã¢ã«ãã³é žèªå°äœ
ã¯ããã®ååå ã«æ°Žé žåºãæããŠãããããã¢ã«
ã³ãŒã«é¡ãšã®çµåããç¹ã«èæ ®ããªããŠããå®å®
æ§ã®ããä¹³åçµæç©ãäžããããŸãçšãããã¢ã«
ãã³é žèªå°äœã®ååéãéžæããäºã«ãããä¹³å
çµæç©ã®ç²åºŠã®èª¿æŽãå¯èœã§ãããå®éã®å粧å
ã®è£œå圢æ ã«ãããŠãä¹³ååãžã®ãã¢ã«ãã³é žé
åãšãã圢ããæ¯èŒç容æã§ããäžãåæã«ä»æ
åãšã®çµã¿åããã«ãå¹ ãã§ãããšããå©ç¹ãåŸ
ãããã ããã«ãæ¬çºæã®ãã¢ã«ãã³é žèªå°äœã¯ãéåžž
ã«ããããä¹³åèœåã³ãã®å®å®åäœçšããããç¹
ã«ãåç¬ã§ã®ïŒ¯ïŒïŒ·åä¹³åç³»ãžã®æ·»å ã«ãããŠã
æ¯èŒçååéã®äœããã¢ã«ãã³é žèªå°äœã«ããè¯
奜ãªä¹³åå®å®æ§ãåŸãããããã¢ã«ãã³é žèªå°äœ
ã®ååéã倧ãããããšä¹³åç²åã®åéãèµ·ãã€
ãŠãä¹³åç³»ãäžå®å®ã«ãªãçã®åé¡ãããã®ã§ã
ã¢ã«ãã³é žèªå°äœã®äœ¿çšã«ããã€ãŠã¯ããã®åå
éããä¹³åç²åãµã€ãºåã³ä¹³åæ¹æ³ã®éžæãé©åœ
ã«è¡ãããšéèŠã§ããããããã®ããšãèæ ®ãã
ããšã«ãããå®å®æ§ã®ããä¹³åç³»ãåŸãããšãã§
ãããä¹³åç³»ã®å®å®åããã€ã¯ããšãã«ãžãšã³å
ã«ããè¡ãå Žåã«ã¯ãä¹³åç²åã®è¡šé¢ç©ã®å¢å€§ã«
ãããçé¢æŽ»æ§å€ã®äœ¿çšéã®å¢å ãå¿ èŠãšããã
ããæ¬çºæã®ãã¢ã«ãã³é žèªå°äœã«ããå®å®åã§
ã¯ããã®ãããªçé¢æŽ»æ§å€ã®äœ¿çšéã®å¢å ã¯é²æ¢
ããããããªãã¡ãç¹ã«è£ 眮çã«é倧ãªãã€ã¯ã
ãšãã«ãžãšã³åãå³ããªããšãä»»æã«éžæããã
ãã¢ã«ãã³é žèªå°äœã«ãããä¹³åç³»ã®å®å®åãå³
ãããšãã§ãããããŸãäžå®ã®ä¹³åæ¹æ³ãéžæã
ãéã«ããã¢ã«ãã³é žèªå°äœã®ååãµã€ãºã®éžæ
ã«ãããä¹³åç²åç³»ã®åäžæ§åã³ä¹³åç²ååŸãå¶
埡ããããšãå¯èœã§ããã æ¬çºæã«ãããã¢ã«ãã³é žèªå°äœã¯ãä¹³åç©ã®
äžã€ã®åœ¢æ ã§ãããªããœãŒã ã®å®å®åã«ãããã
å¹æã瀺ãããã¢ã«ãã³é žããªããœãŒã ã®è¡šé¢è¢«
èŠã®ç®çã§çšããäŸã¯ãç¹éæ59â210013å·å ¬å ±
ã«ç€ºãããŠãããããã®å Žåã¯ãã«ã«ããã·ã¡ã
ã«åãã¢ã«ãã³é žãšãªããœãŒã æ§æã¬ã·ãã³ãšã®
éé»çãªçµåãè¡ã€ãŠããã«éãããåšå²ã®PHã
å¡©æ¿åºŠã«ãããã®è¢«èŠã¯å®¹æã«è§£é¢ããŠããŸãã
æ¬çºæã®ãã¢ã«ãã³é žèªå°äœã氎溶æ§è¢å è²çŽ ã®
ã«ã«ã»ã€ã³ãå å ãããªããœãŒã ã®è¢«èŠã«çšãã
æã«ã¯ããã¹ããªããŒãŒïŒ€ã«ãã€ãŠãããããã
ã«ã«ã»ã€ã³ã®æŸåºãæå¶ãããã æ¬çºæã®ãã¢ã«ãã³é žèªå°äœã¯ãä¹³åå€åã³ä¹³
åå®å®å€ãšããŠãä¹³åçµæç©ãæ±ãåéãäŸã
ã°ãå粧åãå»è¬åãå»è¬éšå€éšããã€ã¬ã¿ãªãŒ
補åãæŽæµå€ãé£åçã®åéã«æå©ã«å©çšãã
ãã ïŒå®æœäŸïŒ æ¬çºæãå®æœäŸã«ãã€ãŠæŽã«è©³çŽ°ã«èª¬æããã åèäŸ ïŒ 500mlã®ãã¹åãã©ã¹ã³äžã§ãã¢ã«ãã³é žãã
ãªãŠã ïŒå¹³åååé2000000ïŒ0.2ïœïŒ0.2ïŒ401ïŒ
5.0Ã10-4ã¢ã«ç³åäœïŒãç¡æ°Žãžã¡ãã«ãã«ã ã¢
ãã200mlã«50ã60âã§å æž©ããªããåæ£ããã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããç¡æ°Žã
ãªãžã³1.6gïŒ2.0Ã10-2ã¢ã«ïŒåã³ãã«ããã€ã«ã¯
ãã©ã€ã4.5gïŒ2.0Ã10-2ã¢ã«ïŒãç¡æ°Žãžã¡ãã«ã
ã«ã ã¢ããã®é©éã«æº¶è§£ãããã®ãå ããããã
ã60âã§ïŒæéä¿ã¡ããã®åŸå®€æž©ã«ãŠïŒã±æéæŸ
眮ãããïŒã±æåŸãåå¿æ··åç©ãå床60âã«30å
éå æž©ããå·åŽããªããã¡ã«æžæ¿ç©ãæ¿Ÿéããã
ãã®åå¿çæç©ãã¢ã»ãã³åã³ã¯ãããã«ã ã§æŽ
æµããæžå§äžãå ç±ä¹Ÿç¥ããã 也ç¥åŸã®åå¿çæç©ã®IRåã³NMRã枬å®ã
ãããã®åå¿çæç©ã®IRåæçµæã«ãããŠã¯ã
1740cm-1ã«ã«ã«ããã«åºã®åžåãèŠãããããã«
ãããšã¹ãã«åœ¢æã®ç¢ºèªãããããŸãåæã«1810
cm-1ã«ãåžåãèªããã°ã«ã¯ãã³é žéšã®ã«ã«ãã
ã·ã«åºã«é žç¡æ°Žç©ã圢æãããŠããããšã確èªã
ãããŸãããã®åå¿çæç©ã®NMRåæçµæã«ã
ããŠã¯ã0.9ppmåã³1.28ppmã«ãã«ããã€ã«åº
ã®ãããã³ã®ããŒã¯ã確èªããã ãŸãããã®åå¿çæç©ã«ãããèèªé žãšã¹ãã«
ãååšããããšãããããããµã é žéæ³ã«ãã€ãŠ
次ã®ããã«ããŠç¢ºèªããããŸãè©ŠæãšããŠåå¿ç
æç©ïŒmgã粟秀ãããšã¿ããŒã«0.6mlã«æžæ¿ããã
ãã®æžæ¿æ¶²ã«ãå¥ã«èª¿æŽããã¢ã«ã«ãªæ§ãããã
ã·ã«ã¢ãã³æ¶²1.25mlãæ·»å ãã65âã§ïŒåéå ç±
ããã ãªããã¢ã«ã«ãªæ§ããããã·ã«ã¢ãã³æ¶²ã¯æ¬¡ã®
ããã«ããŠèª¿æŽãããå¡©é žããããã·ã«ã¢ãã³
2gã粟補氎2.5mlã«æº¶è§£ããç¡æ°Žãšã¿ããŒã«ã§50
mlãšãããã®ã液ãšããæ°Žé žåãããªãŠã 4g
ã粟補氎2.5mlã«æº¶è§£ããç¡æ°Žãšã¿ããŒã«50mlãš
ãããã®ã液ãšããã䜿çšçŽåã«ïŒ¡æ¶²ãšïŒ¢æ¶²ãš
ãçéæ··åããçããå¡©åãããªãŠã ãé å¿é€å»
ããŠäœ¿çšæ¶²ãšããã 次ã«ãåèš65âã§å ç±ããåå¿æ¶²ã宀枩ã§ïŒå
éå·åŽããåŸããã®åå¿æ¶²ã«éå¡©çŽ é žé溶液ïŒé
å¡©çŽ é žç¬¬äºéå æ°Žå¡©5gã70ïŒ éå¡©çŽ é ž10mlãšç²Ÿ
補氎10mlã«æº¶è§£ããå·ãšã¿ããŒã«ã§100mlãšãã
ãã®ãå·æä¿å液ãšãããã®ä¿å液ïŒmlãš70ïŒ é
å¡©çŽ é žïŒmlãåããå·ãšã¿ããŒã«ã§100mlãšãã
ãã®ïŒ3.15mlãæŽã«æ·»å ããŠãããæ¹æããŠ30å
åŸã«ã530nmã«ãããçºè²åžå 床ã枬å®ãããæ¯
èŒã®ããã®è©Šæãå«ãŸãªããã©ã³ã¯ã®åžå 床ã
0.072ã§ããã«ã®ã«å¯Ÿããè©ŠæïŒmgãå«æããã
ã®ã®åžå 床ã¯1.79ã§ããããšã枬å®ãããèèªé ž
ãšã¹ãã«ã®ååšã確èªãããã ãã®åå¿çæç©ã§ãããã«ããã€ã«åºå°å ¥ãã¢
ã«ãã³é žèªå°äœïŒã¯æ¬¡ã®ãããªååæ§é ãæãã
ããšã確èªãããããŸããã®ãã®ã¯æ°Žäžæº¶æ§ã®ã
ã®ã§ãã€ãã
ãåã
ïŒåŒäžãã¯ãã«ããã€ã«åºãæå³ããïœã¯
5000ã§ããïŒ åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªãããïŒèŠå®æ°Žé žåãããªãŠã 液ããã¹ããŒ
ã«ããããã«ãŠïŒæ»Žå ããã宀枩ã«ãŠæ¹æãç¶
ãã12æéåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçº
也åºç©ãåŸãããã®ä¹Ÿåºç©ã«ç²Ÿè£œæ°Ž30mlãå ãã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããïŒèŠå®
å¡©é žã«ãŠPHã7.0調æŽããããã®åŸã60âã§å æž©
溶解ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ãããæ¿Ÿ
液ãå ç±ä¹Ÿç¥ãããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³
é žèªå°äœïŒãåŸãã ãã®çæç©ã®IR枬å®ãè¡ã€ãçµæã1810cm-1
ã®åžåã®æ¶å€±ãš1610cm-1ã®åžåã®ååºçŸã«ããã
é žç¡æ°Žç©ã®å解ãšã«ã«ãã³é žãããªãŠã ã®åçã
確èªããããŸãã1745cm-1ã®åžåã®æžå°ãããé ž
ç¡æ°Žç©ã®æ¶å€±ãšãšãã«ãšã¹ãã«ã®éšåçãªå解ã
確èªãããããã«ããã®çæç©ã®ãããããµã é ž
éæ³ã«ããçºè²åžå 床ã¯ãè©ŠæéïŒmgã§0.315ãš
ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ãã
ãïŒåã®ïŒãšãšã¹ãã«ã®å°å ¥æ¯çã¯å°ãããªã€ã
ããšã確èªããã åèšãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ
ã¯æ¬¡ã®ãããªååŠæ§é ãæããããšã確èªããã
ãŸãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæ°Žæº¶æ§ã瀺ã
ãã
5000ã§ããïŒ åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªãããïŒèŠå®æ°Žé žåãããªãŠã 液ããã¹ããŒ
ã«ããããã«ãŠïŒæ»Žå ããã宀枩ã«ãŠæ¹æãç¶
ãã12æéåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçº
也åºç©ãåŸãããã®ä¹Ÿåºç©ã«ç²Ÿè£œæ°Ž30mlãå ãã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããïŒèŠå®
å¡©é žã«ãŠPHã7.0調æŽããããã®åŸã60âã§å æž©
溶解ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ãããæ¿Ÿ
液ãå ç±ä¹Ÿç¥ãããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³
é žèªå°äœïŒãåŸãã ãã®çæç©ã®IR枬å®ãè¡ã€ãçµæã1810cm-1
ã®åžåã®æ¶å€±ãš1610cm-1ã®åžåã®ååºçŸã«ããã
é žç¡æ°Žç©ã®å解ãšã«ã«ãã³é žãããªãŠã ã®åçã
確èªããããŸãã1745cm-1ã®åžåã®æžå°ãããé ž
ç¡æ°Žç©ã®æ¶å€±ãšãšãã«ãšã¹ãã«ã®éšåçãªå解ã
確èªãããããã«ããã®çæç©ã®ãããããµã é ž
éæ³ã«ããçºè²åžå 床ã¯ãè©ŠæéïŒmgã§0.315ãš
ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ãã
ãïŒåã®ïŒãšãšã¹ãã«ã®å°å ¥æ¯çã¯å°ãããªã€ã
ããšã確èªããã åèšãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ
ã¯æ¬¡ã®ãããªååŠæ§é ãæããããšã確èªããã
ãŸãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæ°Žæº¶æ§ã瀺ã
ãã
ãåã
ïŒåŒäžãã¯ãã«ããã€ã«åºãæå³ããïœã¯
5000ã§ããïŒã åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã«åèäŸïŒãšåæ§ã®ã¢ã«ã«ãªåŠçãããããåŠ
çæéãïŒæéãšããããã®çæç©ããã«ããã€
ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãšããã ãã®çæç©ã®IR枬å®ãè¡ã€ãçµæãåèäŸïŒ
ãšåæ§ã«é žç¡æ°Žç©ã¯æ¶å€±ããããšãæèªãããã
ãšã¹ãã«ã®æ®åçã¯ã1745cm-1ã®åžåã®æžå°ã®çš
床ãåèäŸïŒããå°ãªãããšããé«ããªã€ãŠã
ããããã«ãã®çæç©ã®ãããããµã é žéæ³ã«ã
ãçºè²åžå 床ã¯ãè©ŠæéïŒmgã§0.855ãšãã«ãã
ã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ãããïŒåã®
ïŒã®ãšã¹ãã«å°å ¥çã瀺ããããŸãããã®ãã®ã¯
ïŒïŒšâNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢ã»ã
ã«åºã®æ¯çã¯ïŒïŒ34ã§ãã€ãã ãã®ãã¢ã«ãã³é žèªå°äœïŒãããã«ããã€ã«åº
å°å ¥ãã¢ã«ãã³é žèªå°äœïŒãšåãååŠæ§é ãæã
ãããšã確èªãããããŸãããã®ãã®ã¯æ°Žã«å¯Ÿã
ãŠè¯å¥œãªæ°Žæº¶æ§ã瀺ããã åèäŸ ïŒ 500mlã®ãã¹åãã©ã¹ã³äžã§ãã¢ã«ãã³é žãã
ãªãŠã ïŒå¹³åååé2000000ïŒ0.5ïœïŒ0.5ïŒ401ïŒ
1.25Ã10-3ã¢ã«ç³åäœïŒãç¡æ°Žãžã¡ãã«ãã«ã ã¢
ãã200mlã«50ã60âã§å æž©ããªããåæ£ããã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããç¡æ°Žã
ãªãžã³11.3gïŒïŒÃ10-2ã¢ã«ïŒåã³ãã«ããã€ã«ã¯
ãã©ã€ã3.9gïŒïŒÃ10-2ã¢ã«ïŒãç¡æ°Žãžã¡ãã«ã
ã«ã ã¢ããã®é©éã«æº¶è§£ãããã®ãå ããããã
ã60âã§ïŒæéä¿ã¡ããã®åŸå®€æž©ã«ãŠïŒæ¥éæŸçœ®
ãããïŒæ¥åŸãåå¿æ··åç©ãå床60âã«30åéå
æž©ããå·åŽããªããã¡ã«æžæ¿ç©ãæ¿Ÿéããããã®
åå¿çæç©ãã¢ã»ãã³åã³ã¯ãããã«ã ã§æŽæµ
ããæžå§äžãå ç±ä¹Ÿç¥ããã ãã®åå¿çæç©ã®IRãšNMRã®æž¬å®çµæããã
åèäŸïŒã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°
äœïŒãšåãæ§é ã®ãã®ã§ããããçæ°Žæ§åºã®å°å ¥
çãæããã«äœãããšã確èªããããã®çæç©ã®
ãããããµã é žéæ³ã«ããçºè²åžå 床ã¯ãè©Šæé
ïŒmgã§0.360ãšãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é ž
èªå°äœïŒã®ãããïŒåã®ïŒã®ãšã¹ãã«å°å ¥çã瀺
ããã ãã®åå¿çæç©ããã«ããã€ã«åºå°å ¥ãã¢ã«ã
ã³é žèªå°äœïŒãšããããã®ãã®ã¯æ°Žã«å¯ŸããŠäžæº¶
æ§ã瀺ããã åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªããã28ïŒ ã¢ã³ã¢ãã¢æ°Žããã¹ããŒã«ããã
ãã«ãŠïŒæ»Žããããã宀枩ã«ãŠæ¹æãç¶ãã12æ
éåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçºä¹Ÿåºç©ã
åŸãããããžç²Ÿè£œæ°Ž30mlãå ãã60âã§å 枩溶解
ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ããã濟液ã ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªããã28ïŒ ã¢ã³ã¢ãã¢æ°Žããã¹ããŒã«ããã
ãã«ãŠïŒæ»Žããããã宀枩ã«ãŠæ¹æãç¶ãã12æ
éåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçºä¹Ÿåºç©ã
åŸãããããžç²Ÿè£œæ°Ž30mlãå ãã60âã§å 枩溶解
ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ããã濟液ã
å ç±ä¹Ÿç¥ãããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒãåŸãã ãã®çæç©ã®IRåæçµæã«ãããŠã1810cm-1
ã®åžåã®æ¶å€±ã«ãããé žç¡æ°Žç©ã®å解ã確èªãã
ãšåæã«ã1640cm-1ãã1690cm-1ã®åžå垯ã«å€å
ããããã°ã«ã¯ãã³é žã®ã«ã«ããã·ã«åºãäžçŽã¢
ããïŒCONH2ïŒã圢æããŠããããšã確èªã
ãããã®çæç©ã®ãããããµã é žéæ³ã«ããçºè²
åžå 床ã¯ãè©ŠæéïŒmgã§0.010ãšãã«ããã€ã«å°
å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ããã170åã®ïŒã§ã
ã€ãããŸãããã®ãã®ã®1âNMRã«ãããã«
ããã€ã«åºïŒïŒ®âã¢ã»ãã«åºã®æ¯çã¯ïŒïŒ118ã§
ãã€ãããã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒã¯ãäžèšååŠæ§é ãæããããšã確èªãã
ãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ã
ãã
5000ã§ããïŒã åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã«åèäŸïŒãšåæ§ã®ã¢ã«ã«ãªåŠçãããããåŠ
çæéãïŒæéãšããããã®çæç©ããã«ããã€
ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãšããã ãã®çæç©ã®IR枬å®ãè¡ã€ãçµæãåèäŸïŒ
ãšåæ§ã«é žç¡æ°Žç©ã¯æ¶å€±ããããšãæèªãããã
ãšã¹ãã«ã®æ®åçã¯ã1745cm-1ã®åžåã®æžå°ã®çš
床ãåèäŸïŒããå°ãªãããšããé«ããªã€ãŠã
ããããã«ãã®çæç©ã®ãããããµã é žéæ³ã«ã
ãçºè²åžå 床ã¯ãè©ŠæéïŒmgã§0.855ãšãã«ãã
ã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ãããïŒåã®
ïŒã®ãšã¹ãã«å°å ¥çã瀺ããããŸãããã®ãã®ã¯
ïŒïŒšâNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢ã»ã
ã«åºã®æ¯çã¯ïŒïŒ34ã§ãã€ãã ãã®ãã¢ã«ãã³é žèªå°äœïŒãããã«ããã€ã«åº
å°å ¥ãã¢ã«ãã³é žèªå°äœïŒãšåãååŠæ§é ãæã
ãããšã確èªãããããŸãããã®ãã®ã¯æ°Žã«å¯Ÿã
ãŠè¯å¥œãªæ°Žæº¶æ§ã瀺ããã åèäŸ ïŒ 500mlã®ãã¹åãã©ã¹ã³äžã§ãã¢ã«ãã³é žãã
ãªãŠã ïŒå¹³åååé2000000ïŒ0.5ïœïŒ0.5ïŒ401ïŒ
1.25Ã10-3ã¢ã«ç³åäœïŒãç¡æ°Žãžã¡ãã«ãã«ã ã¢
ãã200mlã«50ã60âã§å æž©ããªããåæ£ããã
ãã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æããªãããç¡æ°Žã
ãªãžã³11.3gïŒïŒÃ10-2ã¢ã«ïŒåã³ãã«ããã€ã«ã¯
ãã©ã€ã3.9gïŒïŒÃ10-2ã¢ã«ïŒãç¡æ°Žãžã¡ãã«ã
ã«ã ã¢ããã®é©éã«æº¶è§£ãããã®ãå ããããã
ã60âã§ïŒæéä¿ã¡ããã®åŸå®€æž©ã«ãŠïŒæ¥éæŸçœ®
ãããïŒæ¥åŸãåå¿æ··åç©ãå床60âã«30åéå
æž©ããå·åŽããªããã¡ã«æžæ¿ç©ãæ¿Ÿéããããã®
åå¿çæç©ãã¢ã»ãã³åã³ã¯ãããã«ã ã§æŽæµ
ããæžå§äžãå ç±ä¹Ÿç¥ããã ãã®åå¿çæç©ã®IRãšNMRã®æž¬å®çµæããã
åèäŸïŒã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°
äœïŒãšåãæ§é ã®ãã®ã§ããããçæ°Žæ§åºã®å°å ¥
çãæããã«äœãããšã確èªããããã®çæç©ã®
ãããããµã é žéæ³ã«ããçºè²åžå 床ã¯ãè©Šæé
ïŒmgã§0.360ãšãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é ž
èªå°äœïŒã®ãããïŒåã®ïŒã®ãšã¹ãã«å°å ¥çã瀺
ããã ãã®åå¿çæç©ããã«ããã€ã«åºå°å ¥ãã¢ã«ã
ã³é žèªå°äœïŒãšããããã®ãã®ã¯æ°Žã«å¯ŸããŠäžæº¶
æ§ã瀺ããã åèäŸ ïŒ ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªããã28ïŒ ã¢ã³ã¢ãã¢æ°Žããã¹ããŒã«ããã
ãã«ãŠïŒæ»Žããããã宀枩ã«ãŠæ¹æãç¶ãã12æ
éåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçºä¹Ÿåºç©ã
åŸãããããžç²Ÿè£œæ°Ž30mlãå ãã60âã§å 枩溶解
ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ããã濟液ã ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒ100
mgã50ml容ãã¹åãã©ã¹ã³ã«åããã¯ãããã«ã
30mlã«åæ£ããããã°ãããã¯ã¹ã¿ãŒã©ãŒã§æ¹æ
ããªããã28ïŒ ã¢ã³ã¢ãã¢æ°Žããã¹ããŒã«ããã
ãã«ãŠïŒæ»Žããããã宀枩ã«ãŠæ¹æãç¶ãã12æ
éåŸãããŒã¿ãªãŒãšããã¬ãŒã¿ãŒã§èžçºä¹Ÿåºç©ã
åŸãããããžç²Ÿè£œæ°Ž30mlãå ãã60âã§å 枩溶解
ãããæ°Žäžæº¶æ§ç©ã¯æ¿Ÿéã«ããé€å»ããã濟液ã
å ç±ä¹Ÿç¥ãããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒãåŸãã ãã®çæç©ã®IRåæçµæã«ãããŠã1810cm-1
ã®åžåã®æ¶å€±ã«ãããé žç¡æ°Žç©ã®å解ã確èªãã
ãšåæã«ã1640cm-1ãã1690cm-1ã®åžå垯ã«å€å
ããããã°ã«ã¯ãã³é žã®ã«ã«ããã·ã«åºãäžçŽã¢
ããïŒCONH2ïŒã圢æããŠããããšã確èªã
ãããã®çæç©ã®ãããããµã é žéæ³ã«ããçºè²
åžå 床ã¯ãè©ŠæéïŒmgã§0.010ãšãã«ããã€ã«å°
å ¥ãã¢ã«ãã³é žèªå°äœïŒã®ããã170åã®ïŒã§ã
ã€ãããŸãããã®ãã®ã®1âNMRã«ãããã«
ããã€ã«åºïŒïŒ®âã¢ã»ãã«åºã®æ¯çã¯ïŒïŒ118ã§
ãã€ãããã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒã¯ãäžèšååŠæ§é ãæããããšã確èªãã
ãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ã
ãã
ãåã
ïŒåŒäžãã¯ãã«ããã€ã«åºãæå³ããïœã¯
5000ã§ããïŒ åèäŸ ïŒ åèäŸïŒã«ãããŠããã¢ã«ãã³é žãããªãŠã ãš
ããŠå¹³åååéçŽ20000ã®ãã®ãçšãã以å€ã¯å
æ§ã«ããŠåŒ(1)ïŒäœããïœïŒ50ïŒã§è¡šãããããã«
ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãåŸããã
ã®ãã®ã®ãããããµã é žéæ³ã«ããçºè²åžå 床
ã¯ãïŒmgåœã0.083ã瀺ããããŸãããã®ãã®ã®1
âNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢ã»ãã«
åºã®æ¯çã¯ïŒïŒ33ã§ãã€ãããã®ãã®ã¯æ°Žã«å¯Ÿã
ãŠããäžæº¶æ§ã瀺ããã åèäŸ ïŒ åèäŸïŒã«ãããŠããã«ããã€ã«åºå°å ¥ãã¢ã«
ãã³é žèªå°äœãšããŠãåèäŸïŒã§åŸããããã®ïŒ
ãçšãã以å€ã¯åæ§ã«ããŠåŒ(2)ïŒäœããïœïŒ50ïŒ
ã§è¡šãããããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒãåŸãããã®ãã®ã®ãããããµã é žéæ³ã«
ããçºè²åžå 床ã¯ãïŒmgåœã0.006ã瀺ããããŸ
ãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ã
ãã åèäŸ ïŒ åèäŸïŒã«ãããŠããã«ããã€ã«åºå°å ¥ãã¢ã«
ãã³é žèªå°äœãšããŠãåèäŸïŒã§ç€ºãããã®ïŒã
çšãã以å€ã¯åæ§ã«ããŠåŒ(2)ïŒäœããïœïŒ50ïŒã§
è¡šãããããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°
äœïŒãåŸãããã®ãã®ã®ãããããµã é žéæ³ã«ã
ãçºè²åžå 床ã¯ãïŒmgåœã0.084ã瀺ããããŸãã
ãã®ãã®ã®1âNMRã«ãããã«ããã€ã«åºïŒ
âã¢ã»ãã«åºã®æ¯çã¯ïŒïŒ55ã§ãã€ãããã®ã
ã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ããã åèäŸ ïŒ åèäŸïŒã«ãããŠããã¢ã«ãã³é žãããªãŠã ãš
ããŠãå¹³åååéçŽ20000ã®ãã®ãçšãã以å€ã¯
åæ§ã«ããŠåŒ(1)ïŒäœããïœïŒ50ïŒã§ç€ºãããæ§é
ã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãåŸ
ãããã®ãã®ã®ãããããµã é žéæ³ã«ããçºè²åž
å 床ã¯ãïŒmgåœã0.032ã瀺ããããŸãããã®ã
ã®ã®1âNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢
ã»ãã«åºã®æ¯çã¯ïŒïŒ37ã§ãã€ãããã®ãã®ã¯æ°Ž
ã«å¯ŸããŠããäžæº¶æ§ã瀺ããã å®æœäŸ ïŒ åèšåèäŸïŒã§åŸããã«ããã€ã«åãã¢ã«ãã³
é žïŒä»¥äžãHA2ââNAãšãç¥èšããïŒã®0.5
ïŒ ã®æ°Žæº¶æ¶²ã調補ããããã«å€§è±æ²¹ããã®çµæ¿åºŠ
ã0.01ïŒ0.02ïŒ0.03åã³0.04ïŒïŒ ïŒã«ãªãããã«å
ãã倧è±æ²¹ã«å¯Ÿãããã«ããã€ã«åãã¢ã«ãã³é ž
ã®å¯æº¶åèœã枬å®ãããæ¯èŒãšããŠãããªãªãã·
ãšãã¬ã³åããã·æ²¹ïŒæ¥å ã±ãã«ã«ãº(æ ª)補ã
HC060ïŒåã³ããªãªãã·ãšãã¬ã³ãœã«ãã¿ã³ã¢
ããªã¬ã€ã³ïŒTween80ïŒãåãã0.5ïŒ ã®æ¿åºŠã§
çšããå¯Ÿç §ãšããŠç¡æ·»å ã§ã®å¯æº¶åèœã®æž¬å®ãè¡
ã€ããå¯æº¶åèœã®æž¬å®ã¯ã溶液ã40Wã3minã®
è¶ é³æ³¢åŠçããåŸãããã«æ³¢é·640nmã®å ãç §å°
ãããã®ééçã枬å®ããããšã«ããè¡ã€ããé
éçã®å€§ãããã®ã»ã©å¯æº¶åèœã倧ããããšã瀺
ãããã®çµæãè¡šâïŒã«ç€ºãã ãã®è¡šâïŒã«ç€ºããçµæãããããããã«ãã
ãªãªãã·ãšãã¬ã³åããã·æ²¹åã³Tween80ã¯ã
倧è±æ²¹æ¿åºŠãäžæããã«ã€ããŠãééçã®äœäžã¯
ããŸãèªããããªãã€ããããã«å¯ŸããHA2â
âNAã§ã¯ééçã®å€§ããªäœäžããããå¯Ÿç §ãš
ã»ãŒåæ§ã§ãã€ãããã®ããšããããã«ããã€ã«
åãã¢ã«ãã³é žã«ã¯å€§è±æ²¹ã«å¯Ÿããå¯æº¶åèœã¯ãª
ããšãããããããã以äžã®å®æœäŸã§ã瀺ããã
ããã«æ²¹ãšæ°Žã®åé¢ã¯ãªããå¯æº¶åèœããªããšã
ãçµæã¯ãããªãã¡ãä¹³åç²ååŸãããå°ãããª
ã€ãŠããªãããšã瀺ããŠãããå®éã倧è±æ²¹ã®çµ
æ¿åºŠ0.04ïŒ ã«ãããä¹³åç²ååŸã®æ¯èŒã«ãããŠã
Tween80ã§ã¯145nmã«å¯ŸããHA2ââNAã§
265nmãšå€§ããã€ãããã®ããšããããã«ããã€
ã«åãã¢ã«ãã³é žãä¹³åå®å®æ§ã«å¯äžããããšã¯
æããã§ãããä¹³åå®å®åã«ãã€ã¯ããšãã«ãžãš
ã³åãå¿ ããšãå¿ èŠãšãããªãããšãããããåŸ
ã€ãŠãæ¬çºæã®ãã¢ã«ãã³é žèªå°äœãçšããããš
ã«ããããã€ã¯ããšãã«ãžãšã³åè£ çœ®çç¥ããæ©
械çããšãã«ã®ãŒçãªæ倱ãé²ãããšãã§ããã
5000ã§ããïŒ åèäŸ ïŒ åèäŸïŒã«ãããŠããã¢ã«ãã³é žãããªãŠã ãš
ããŠå¹³åååéçŽ20000ã®ãã®ãçšãã以å€ã¯å
æ§ã«ããŠåŒ(1)ïŒäœããïœïŒ50ïŒã§è¡šãããããã«
ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãåŸããã
ã®ãã®ã®ãããããµã é žéæ³ã«ããçºè²åžå 床
ã¯ãïŒmgåœã0.083ã瀺ããããŸãããã®ãã®ã®1
âNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢ã»ãã«
åºã®æ¯çã¯ïŒïŒ33ã§ãã€ãããã®ãã®ã¯æ°Žã«å¯Ÿã
ãŠããäžæº¶æ§ã瀺ããã åèäŸ ïŒ åèäŸïŒã«ãããŠããã«ããã€ã«åºå°å ¥ãã¢ã«
ãã³é žèªå°äœãšããŠãåèäŸïŒã§åŸããããã®ïŒ
ãçšãã以å€ã¯åæ§ã«ããŠåŒ(2)ïŒäœããïœïŒ50ïŒ
ã§è¡šãããããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèª
å°äœïŒãåŸãããã®ãã®ã®ãããããµã é žéæ³ã«
ããçºè²åžå 床ã¯ãïŒmgåœã0.006ã瀺ããããŸ
ãããã®ãã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ã
ãã åèäŸ ïŒ åèäŸïŒã«ãããŠããã«ããã€ã«åºå°å ¥ãã¢ã«
ãã³é žèªå°äœãšããŠãåèäŸïŒã§ç€ºãããã®ïŒã
çšãã以å€ã¯åæ§ã«ããŠåŒ(2)ïŒäœããïœïŒ50ïŒã§
è¡šãããããã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°
äœïŒãåŸãããã®ãã®ã®ãããããµã é žéæ³ã«ã
ãçºè²åžå 床ã¯ãïŒmgåœã0.084ã瀺ããããŸãã
ãã®ãã®ã®1âNMRã«ãããã«ããã€ã«åºïŒ
âã¢ã»ãã«åºã®æ¯çã¯ïŒïŒ55ã§ãã€ãããã®ã
ã®ã¯æ°Žã«å¯ŸããŠè¯å¥œãªæº¶è§£æ§ã瀺ããã åèäŸ ïŒ åèäŸïŒã«ãããŠããã¢ã«ãã³é žãããªãŠã ãš
ããŠãå¹³åååéçŽ20000ã®ãã®ãçšãã以å€ã¯
åæ§ã«ããŠåŒ(1)ïŒäœããïœïŒ50ïŒã§ç€ºãããæ§é
ã®ãã«ããã€ã«åºå°å ¥ãã¢ã«ãã³é žèªå°äœïŒãåŸ
ãããã®ãã®ã®ãããããµã é žéæ³ã«ããçºè²åž
å 床ã¯ãïŒmgåœã0.032ã瀺ããããŸãããã®ã
ã®ã®1âNMRã«ãããã«ããã€ã«åºïŒïŒ®âã¢
ã»ãã«åºã®æ¯çã¯ïŒïŒ37ã§ãã€ãããã®ãã®ã¯æ°Ž
ã«å¯ŸããŠããäžæº¶æ§ã瀺ããã å®æœäŸ ïŒ åèšåèäŸïŒã§åŸããã«ããã€ã«åãã¢ã«ãã³
é žïŒä»¥äžãHA2ââNAãšãç¥èšããïŒã®0.5
ïŒ ã®æ°Žæº¶æ¶²ã調補ããããã«å€§è±æ²¹ããã®çµæ¿åºŠ
ã0.01ïŒ0.02ïŒ0.03åã³0.04ïŒïŒ ïŒã«ãªãããã«å
ãã倧è±æ²¹ã«å¯Ÿãããã«ããã€ã«åãã¢ã«ãã³é ž
ã®å¯æº¶åèœã枬å®ãããæ¯èŒãšããŠãããªãªãã·
ãšãã¬ã³åããã·æ²¹ïŒæ¥å ã±ãã«ã«ãº(æ ª)補ã
HC060ïŒåã³ããªãªãã·ãšãã¬ã³ãœã«ãã¿ã³ã¢
ããªã¬ã€ã³ïŒTween80ïŒãåãã0.5ïŒ ã®æ¿åºŠã§
çšããå¯Ÿç §ãšããŠç¡æ·»å ã§ã®å¯æº¶åèœã®æž¬å®ãè¡
ã€ããå¯æº¶åèœã®æž¬å®ã¯ã溶液ã40Wã3minã®
è¶ é³æ³¢åŠçããåŸãããã«æ³¢é·640nmã®å ãç §å°
ãããã®ééçã枬å®ããããšã«ããè¡ã€ããé
éçã®å€§ãããã®ã»ã©å¯æº¶åèœã倧ããããšã瀺
ãããã®çµæãè¡šâïŒã«ç€ºãã ãã®è¡šâïŒã«ç€ºããçµæãããããããã«ãã
ãªãªãã·ãšãã¬ã³åããã·æ²¹åã³Tween80ã¯ã
倧è±æ²¹æ¿åºŠãäžæããã«ã€ããŠãééçã®äœäžã¯
ããŸãèªããããªãã€ããããã«å¯ŸããHA2â
âNAã§ã¯ééçã®å€§ããªäœäžããããå¯Ÿç §ãš
ã»ãŒåæ§ã§ãã€ãããã®ããšããããã«ããã€ã«
åãã¢ã«ãã³é žã«ã¯å€§è±æ²¹ã«å¯Ÿããå¯æº¶åèœã¯ãª
ããšãããããããã以äžã®å®æœäŸã§ã瀺ããã
ããã«æ²¹ãšæ°Žã®åé¢ã¯ãªããå¯æº¶åèœããªããšã
ãçµæã¯ãããªãã¡ãä¹³åç²ååŸãããå°ãããª
ã€ãŠããªãããšã瀺ããŠãããå®éã倧è±æ²¹ã®çµ
æ¿åºŠ0.04ïŒ ã«ãããä¹³åç²ååŸã®æ¯èŒã«ãããŠã
Tween80ã§ã¯145nmã«å¯ŸããHA2ââNAã§
265nmãšå€§ããã€ãããã®ããšããããã«ããã€
ã«åãã¢ã«ãã³é žãä¹³åå®å®æ§ã«å¯äžããããšã¯
æããã§ãããä¹³åå®å®åã«ãã€ã¯ããšãã«ãžãš
ã³åãå¿ ããšãå¿ èŠãšãããªãããšãããããåŸ
ã€ãŠãæ¬çºæã®ãã¢ã«ãã³é žèªå°äœãçšããããš
ã«ããããã€ã¯ããšãã«ãžãšã³åè£ çœ®çç¥ããæ©
械çããšãã«ã®ãŒçãªæ倱ãé²ãããšãã§ããã
ãè¡šã
å®æœäŸ ïŒ
åçš®ãã«ããã€ã«åãã¢ã«ãã³é
žã®0.5ïŒ
ã®æ°Ž
溶液ã調補ããçµæ¿åºŠ0.5ïŒ ã®å€§è±æ²¹ã«å¯Ÿããä¹³
åèœãšãã®çµæå®å®æ§ã枬å®ãããä¹³åæ¹æ³ã¯ã
æ¯å¹ 25cmãæ¯å60åã§ïŒåéæ¯çªããããšã«ãã
å®æœãããä¹³åç©ã¯ã10ml容ã¬ã©ã¹è£œã·ãªã³ãžã«
10mlãã€å ¥ããã·ãªã³ãžã®å 端ãäžã«ããŠæŸçœ®ã
ãããã®åŸãçµæçã«äžå±€2.5mlãéãã«åãã
æ³¢é·600nmã«ãããééçã枬å®ããããã®éé
çã®çµæå€åãå°ããã»ã©ä¹³åç©ã®å®å®æ§ã®ãã
ããšã瀺ãã ãã«ããã€ã«åãã¢ã«ãã³é žãšããŠHAââ
NAïŒHA5ââNAïŒHA2ââNAïŒHA2â
ã®è©ŠæãçšããããŸããæ¯èŒã®ããã«ãæªä¿®é£Ÿ
ãã¢ã«ãã³é žãšããŠHAïŒHA5ïŒHA2ãçšãã
ãŸããTween80ãçšããŠåæ§ã®å®éšãè¡ã€ãã
ããã«ãç¡æ·»å ã®å®éšãè¡ã€ãããã®çµæãè¡šâ
ïŒã«ç€ºãããHA5ââNAïŒHA2ââNAïŒ
HA2âã¯æ¿åºŠã®å€åãã»ãšãã©ãªãã®ã«å¯Ÿãã
ç¡æ·»å åã³HA5ïŒHA2ã§ã¯èããæ¿åºŠã®äžæã
芳å¯ããããTween80ã§ã¯äžçšåºŠã®æ¿åºŠå€åã§
ãã€ãããªãHAåã³HAââNAã«ãããŠã¯ã
ä¹³åçŽåŸã®å±€åé¢ã極ããŠæ©ããééçã®æž¬å®ã«
äŸããªãã€ãã ããã§çšããHAãšã¯å¹³åååé200äžãHA5
ãšã¯å¹³åååéïŒäžãHA2ãšã¯å¹³åååéïŒäž
ã®ãã¢ã«ãã³é žãèšãããŸãHAââNAïŒ
HA5ââNAïŒHA2ââNAãšã¯ãHAïŒ
HA5åã³HA2ã®ãã¢ã«ãã³é žããèªå°ãããäž
è¬åŒïŒïŒã§ç€ºããããã¢ã«ãã³é žèªå°äœã®ããš
ã§ãããã§ã¯ãåèäŸïŒã«åŸã€ãŠåŸããããã¢ã«
ãã³é žèªå°äœã®Naå¡©ãçšãããããã«ãHA2â
ãšã¯äžè¬åŒïŒïŒã§ç€ºãããèªå°äœã®ããšã§ã
ããåèäŸïŒã§åŸããããã®ãçšããã以äžã®äº
ãããååéãæ°äžçšåºŠã®äžè¬åŒïŒïŒåã³
ïŒïŒã§ç€ºããããã¢ã«ãã³é žèªå°äœã«ã¯ã極ã
ãŠè¯å¥œãªä¹³åèœåã³å®å®åæ§èœããããšãããã
ãªããå¹³åååéã200äžçšåºŠã®ãã®ã«ã€ããŠã¯ã
æ²¹ã®éžæåã³ä¹³åæ¹æ³ã®çµã¿åããã«ãããè¯å¥œ
ãªçµæãåŸãããšãã§ããã
溶液ã調補ããçµæ¿åºŠ0.5ïŒ ã®å€§è±æ²¹ã«å¯Ÿããä¹³
åèœãšãã®çµæå®å®æ§ã枬å®ãããä¹³åæ¹æ³ã¯ã
æ¯å¹ 25cmãæ¯å60åã§ïŒåéæ¯çªããããšã«ãã
å®æœãããä¹³åç©ã¯ã10ml容ã¬ã©ã¹è£œã·ãªã³ãžã«
10mlãã€å ¥ããã·ãªã³ãžã®å 端ãäžã«ããŠæŸçœ®ã
ãããã®åŸãçµæçã«äžå±€2.5mlãéãã«åãã
æ³¢é·600nmã«ãããééçã枬å®ããããã®éé
çã®çµæå€åãå°ããã»ã©ä¹³åç©ã®å®å®æ§ã®ãã
ããšã瀺ãã ãã«ããã€ã«åãã¢ã«ãã³é žãšããŠHAââ
NAïŒHA5ââNAïŒHA2ââNAïŒHA2â
ã®è©ŠæãçšããããŸããæ¯èŒã®ããã«ãæªä¿®é£Ÿ
ãã¢ã«ãã³é žãšããŠHAïŒHA5ïŒHA2ãçšãã
ãŸããTween80ãçšããŠåæ§ã®å®éšãè¡ã€ãã
ããã«ãç¡æ·»å ã®å®éšãè¡ã€ãããã®çµæãè¡šâ
ïŒã«ç€ºãããHA5ââNAïŒHA2ââNAïŒ
HA2âã¯æ¿åºŠã®å€åãã»ãšãã©ãªãã®ã«å¯Ÿãã
ç¡æ·»å åã³HA5ïŒHA2ã§ã¯èããæ¿åºŠã®äžæã
芳å¯ããããTween80ã§ã¯äžçšåºŠã®æ¿åºŠå€åã§
ãã€ãããªãHAåã³HAââNAã«ãããŠã¯ã
ä¹³åçŽåŸã®å±€åé¢ã極ããŠæ©ããééçã®æž¬å®ã«
äŸããªãã€ãã ããã§çšããHAãšã¯å¹³åååé200äžãHA5
ãšã¯å¹³åååéïŒäžãHA2ãšã¯å¹³åååéïŒäž
ã®ãã¢ã«ãã³é žãèšãããŸãHAââNAïŒ
HA5ââNAïŒHA2ââNAãšã¯ãHAïŒ
HA5åã³HA2ã®ãã¢ã«ãã³é žããèªå°ãããäž
è¬åŒïŒïŒã§ç€ºããããã¢ã«ãã³é žèªå°äœã®ããš
ã§ãããã§ã¯ãåèäŸïŒã«åŸã€ãŠåŸããããã¢ã«
ãã³é žèªå°äœã®Naå¡©ãçšãããããã«ãHA2â
ãšã¯äžè¬åŒïŒïŒã§ç€ºãããèªå°äœã®ããšã§ã
ããåèäŸïŒã§åŸããããã®ãçšããã以äžã®äº
ãããååéãæ°äžçšåºŠã®äžè¬åŒïŒïŒåã³
ïŒïŒã§ç€ºããããã¢ã«ãã³é žèªå°äœã«ã¯ã極ã
ãŠè¯å¥œãªä¹³åèœåã³å®å®åæ§èœããããšãããã
ãªããå¹³åååéã200äžçšåºŠã®ãã®ã«ã€ããŠã¯ã
æ²¹ã®éžæåã³ä¹³åæ¹æ³ã®çµã¿åããã«ãããè¯å¥œ
ãªçµæãåŸãããšãã§ããã
ãè¡šã
å®æœäŸ ïŒ
ä¹³åæ¹æ³ãšããŠè¶
é³æ³¢æ³ãçšããïŒïŒ
倧è±æ²¹ã«
察ããä¹³åå®å®æ§ã«ã€ããŠæž¬å®ããããã«ããã€
ã«åãã¢ã«ãã³é žãšããŠHAââNAïŒHA2â
âNAã0.5ïŒ ã®æ¿åºŠã§äœ¿çšãããä¹³åå®å®æ§ã®
枬å®ã¯ã倧èªæ²¹ïŒïŒ ãšæ·»å å€0.5ïŒ ãæ·»å ããæ°Ž
ãã40WãïŒåéã®è¶ é³æ³¢åŠçããåŸãããä¹³å
ç©ãç®çãä»ãè©Šéšç®¡ã«å ¥ãã宀枩ã«ãŠé眮ãã
çµæçã«åœ¢æããŠããæ°Žå±€éšåã®é·ãã枬ãããš
ã«ããè¡ã€ããè¡šâïŒã«ããã®æ°Žå±€éšåã®é·ãã
å šé·ã§å²ã€ãæ°å€ã«100ãæãããã®ãåé¢åºŠ
ïŒïŒ ïŒãšããŠç€ºããã è¡šâïŒã®çµæãããããããã«ãHA2ââ
NAãå ãããã®ã¯æ¥µããŠè¯å¥œãªå®å®æ§ã瀺ã
ããHAââNAãå ãããã®ã¯ç¡æ·»å ãšæ¯ã¹
ãŠãèããäžå®å®æ§ã瀺ããããã®ããšã¯ãHA
ââNAã®ååéã200äžãšå€§ããã®ã«å¯Ÿãã
ä¹³åç²åã匷å¶çã«å°ããããããšããããã
HAââNAååã«ããä¹³åç²åéã®æ¶æ©ãå
éå¡ã®åœ¢æãä¿é²ãããã®ãšèãããããã®ããš
ããããHAââNAãçšããŠä¹³åå®å®åãè¡
ãã«éãã匷å¶çãªãã€ã¯ããšãã«ãžãšã³åãæœ
ãããšã¯éå¹æã§ããããšããããã
察ããä¹³åå®å®æ§ã«ã€ããŠæž¬å®ããããã«ããã€
ã«åãã¢ã«ãã³é žãšããŠHAââNAïŒHA2â
âNAã0.5ïŒ ã®æ¿åºŠã§äœ¿çšãããä¹³åå®å®æ§ã®
枬å®ã¯ã倧èªæ²¹ïŒïŒ ãšæ·»å å€0.5ïŒ ãæ·»å ããæ°Ž
ãã40WãïŒåéã®è¶ é³æ³¢åŠçããåŸãããä¹³å
ç©ãç®çãä»ãè©Šéšç®¡ã«å ¥ãã宀枩ã«ãŠé眮ãã
çµæçã«åœ¢æããŠããæ°Žå±€éšåã®é·ãã枬ãããš
ã«ããè¡ã€ããè¡šâïŒã«ããã®æ°Žå±€éšåã®é·ãã
å šé·ã§å²ã€ãæ°å€ã«100ãæãããã®ãåé¢åºŠ
ïŒïŒ ïŒãšããŠç€ºããã è¡šâïŒã®çµæãããããããã«ãHA2ââ
NAãå ãããã®ã¯æ¥µããŠè¯å¥œãªå®å®æ§ã瀺ã
ããHAââNAãå ãããã®ã¯ç¡æ·»å ãšæ¯ã¹
ãŠãèããäžå®å®æ§ã瀺ããããã®ããšã¯ãHA
ââNAã®ååéã200äžãšå€§ããã®ã«å¯Ÿãã
ä¹³åç²åã匷å¶çã«å°ããããããšããããã
HAââNAååã«ããä¹³åç²åéã®æ¶æ©ãå
éå¡ã®åœ¢æãä¿é²ãããã®ãšèãããããã®ããš
ããããHAââNAãçšããŠä¹³åå®å®åãè¡
ãã«éãã匷å¶çãªãã€ã¯ããšãã«ãžãšã³åãæœ
ãããšã¯éå¹æã§ããããšããããã
ãè¡šã
å®æœäŸ ïŒ
ãã«ããã€ã«åãã¢ã«ãã³é
žã®0.5ïŒ
ã§ã®äœ¿çš
æ¿åºŠã«ããåŸãããçµæ¿åºŠïŒïŒ 倧è±æ²¹ãšãã«ãžãš
ã³ã®çååŠçåŽé¢ã§ã®å®å®æ§ã枬å®ããããšãã«
ãžãšã³ã®èª¿è£œã¯ã40Wã30åéã®è¶ é³æ³¢åŠçã«ã
ãå®æœãããåŸããããšãã«ãžãšã³ã¯ãåå å 床
èšã§ïŒã100ïŒ ã®æž¬å®ã¬ã³ãžã«å ¥ãæ§ã20åã«ç²Ÿ
補氎ã§åžéãã枬å®çšè©Šæãšãããé µçŽ åå¿ã¯è©Š
æïŒmlã«å¯ŸãããªããŒãŒïŒæ±æŽéžé (æ ª)補ïŒ23åäœ
ãæ·»å åŸã37âã«å æž©ããããšã«ããè¡ãªããšãš
ãã«ãçµæçã«æ³¢é·600nmã«ãããééçã®çµæ
å€åã枬å®ããããã®çµæãè¡šâïŒã«ç€ºãããã«
ããã€ã«åãã¢ã«ãã³é žãšããŠHA5ââNAïŒ
HA2ââNAãçšããã è¡šâïŒã®çµæãããããããã«ãç¡æ·»å ãšãã«
ãžãšã³ã«æ¯ã¹ãŠãHA5ââNAïŒHA2ââ
NAã«ãããšãã«ãžãšã³ã¯ãééçã®å€åãå°ã
ãã€ããè¡šâïŒã«ç€ºãã枬å®å€ã¯ãããããããª
ããŒãŒãå ããªããšãã«ãžãšã³ã®çµæçãªééç
ã®å€åãå·®ãåŒããŠãããã®ã§ãããªã°ãªã»ãªã
ããªããŒãŒäœçšã«ããå æ°Žå解ããããšãã«ãžãš
ã³ã®åœ¢æ å€åãåŒãèµ·ãããŠããããšã瀺ããã
ããŠããã®ãªããŒãŒäœçšã«å¯Ÿããæµææ§ããã«ã
ãã€ã«åãã¢ã«ãã³é žæ·»å ãšãã«ãžãšã³ã¯ç€ºãã
ãã®ã§ããã
æ¿åºŠã«ããåŸãããçµæ¿åºŠïŒïŒ 倧è±æ²¹ãšãã«ãžãš
ã³ã®çååŠçåŽé¢ã§ã®å®å®æ§ã枬å®ããããšãã«
ãžãšã³ã®èª¿è£œã¯ã40Wã30åéã®è¶ é³æ³¢åŠçã«ã
ãå®æœãããåŸããããšãã«ãžãšã³ã¯ãåå å 床
èšã§ïŒã100ïŒ ã®æž¬å®ã¬ã³ãžã«å ¥ãæ§ã20åã«ç²Ÿ
補氎ã§åžéãã枬å®çšè©Šæãšãããé µçŽ åå¿ã¯è©Š
æïŒmlã«å¯ŸãããªããŒãŒïŒæ±æŽéžé (æ ª)補ïŒ23åäœ
ãæ·»å åŸã37âã«å æž©ããããšã«ããè¡ãªããšãš
ãã«ãçµæçã«æ³¢é·600nmã«ãããééçã®çµæ
å€åã枬å®ããããã®çµæãè¡šâïŒã«ç€ºãããã«
ããã€ã«åãã¢ã«ãã³é žãšããŠHA5ââNAïŒ
HA2ââNAãçšããã è¡šâïŒã®çµæãããããããã«ãç¡æ·»å ãšãã«
ãžãšã³ã«æ¯ã¹ãŠãHA5ââNAïŒHA2ââ
NAã«ãããšãã«ãžãšã³ã¯ãééçã®å€åãå°ã
ãã€ããè¡šâïŒã«ç€ºãã枬å®å€ã¯ãããããããª
ããŒãŒãå ããªããšãã«ãžãšã³ã®çµæçãªééç
ã®å€åãå·®ãåŒããŠãããã®ã§ãããªã°ãªã»ãªã
ããªããŒãŒäœçšã«ããå æ°Žå解ããããšãã«ãžãš
ã³ã®åœ¢æ å€åãåŒãèµ·ãããŠããããšã瀺ããã
ããŠããã®ãªããŒãŒäœçšã«å¯Ÿããæµææ§ããã«ã
ãã€ã«åãã¢ã«ãã³é žæ·»å ãšãã«ãžãšã³ã¯ç€ºãã
ãã®ã§ããã
ãè¡šã
å®æœäŸ ïŒ
ä¹³åçµæç©ã®å®å®åãæ€èšããäžã§ãä¹³å圢æ
ã®ã²ãšã€ã§ãããªããœãŒã ã®å®å®åããæè¿ã泚
ç®ãããŠãããããã§ãã«ããã€ã«åãã¢ã«ãã³
é žã§è¢«èŠããã¬ã·ãã³ãªããœãŒã ã®ãã¹ããªããŒ
ãŒäœçšæµææ§ãæ€èšããã ãŸãã粟補åµé»ã¬ã·ãã³ïŒæåæ(æ ª)補ïŒãšã³ã¬
ã¹ãããŒã«ã®ã¯ãããã«ã ïŒã¡ã¿ããŒã«æº¶æ¶²ã補
ããäžå®éããã¹åãã©ã¹ã³ã«åããèžçºä¹Ÿåºã
ãããã®åŸã0.4mMã®ã«ã«ã»ã€ã³æ°Žæº¶æ¶²ïŒmlã§
æ°Žåãããã«ããã¯ã¹æ³ã«ãŠç²Ÿè£œåµé»ã¬ã·ãã³å
ã³ã³ã¬ã¹ãããŒã«ã®çµæ¿åºŠïŒãã€ã¯ãã¢ã«ãšãªã
ãªããœãŒã ã調補ããã次ãã§ãã»ãã¢ãŒã¯ã¹ïŒ§
â75ã«ã©ã ã«ãŠããªããœãŒã å æ°Žå±€ã«æªå°å ¥ã®ã«
ã«ã»ã€ã³ãé€å»ãããåŸãããã«ã«ã»ã€ã³å°å ¥ãª
ããœãŒã ãžã®ãã«ããã€ã«åãã¢ã«ãã³é žè¢«èŠ
ã¯ããã«ããã€ã«åãã¢ã«ãã³é žèªå°äœãç²æ«ã®
ãŸãŸãªããœãŒã ãžçµæ¿åºŠïŒãïŒmgïŒmlãšãªããã
æ·»å ããçŽïŒæéç·©åã«æ¹æããããã¹ããªããŒ
ãŒïŒ€ã«å¯Ÿããæµææ§ã®è©Šéšã¯ããªããœãŒã è©ŠæïŒ
mlã«å¯Ÿãããã¹ããªããŒãŒïŒ€ïŒæ±æŽéžé (æ ª)補ïŒ23
åäœãæ·»å ãããªããœãŒã å æ°Žå±€ããæŸåºããŠã
ã氎溶æ§è¢å è²çŽ ã«ã«ã»ã€ã³ã®è¢å ãå±èµ·æ³¢é·
480nmãè¢å æ³¢é·510nmã«ãŠçµæçã«æž¬å®ããã
ãªããå æ°Žå±€ã§å°å ¥ãããŠããã«ã«ã»ã€ã³ã®ç·é
ã¯ã10ïŒ TritonXâ100ã®0.05mlãå ãããªããœ
ãŒã ãç Žå£ããããšã§æ±ããããã®çµæãè¡šâïŒ
ã«ç€ºãããã®å Žåããã«ããã€ã«åãã¢ã«ãã³é ž
èªå°äœãšããŠãHAââNAïŒHA5ââNA
åã³HA2ââNAãçšããããŸããå¯Ÿç §ãšããŠ
被èŠããªããªããœãŒã ãè©Šéšã«äŸãããè¡šâïŒã«
瀺ããçµæãããããããã«ã被èŠããªããªããœ
ãŒã ã«ãããŠãå æ°Žå±€äžã§æ¿åºŠæ¶å ããŠããã«ã«
ã»ã€ã³ã¯ãå šå°å ¥éã®90ïŒ è¿ããŸã§æŸåºãããã
ããã«å¯Ÿãããã«ããã€ã«åãã¢ã«ãã³é žèªå°äœ
被èŠãªããœãŒã ã§ã¯20ã30ïŒ ãŸã§ããæŸåºãããª
ãã€ãããã¹ããªããŒãŒïŒ€ã¯ãã¬ã·ãã³ã®ã³ãªã³
ãšã¹ãã«ãå æ°Žå解ãããªããœãŒã ã®äºååèæ§
é ã«ä¹±ããçãããããã®ã§ããã®çµæãå æ°Žå±€
å°å ¥ç©ãæŸåºããŠãããã®ã§ããããã€ãŠããã«
ããã€ã«åãã¢ã«ãã³é žã¯ããªããœãŒã ã被èŠã
ãããšã§ãã¹ããªããŒãŒïŒ€æµææ§ãæ®çºãããªã
ãœãŒã èæ§é ã®å®å®åããããããã®ã§ããã
ã®ã²ãšã€ã§ãããªããœãŒã ã®å®å®åããæè¿ã泚
ç®ãããŠãããããã§ãã«ããã€ã«åãã¢ã«ãã³
é žã§è¢«èŠããã¬ã·ãã³ãªããœãŒã ã®ãã¹ããªããŒ
ãŒäœçšæµææ§ãæ€èšããã ãŸãã粟補åµé»ã¬ã·ãã³ïŒæåæ(æ ª)補ïŒãšã³ã¬
ã¹ãããŒã«ã®ã¯ãããã«ã ïŒã¡ã¿ããŒã«æº¶æ¶²ã補
ããäžå®éããã¹åãã©ã¹ã³ã«åããèžçºä¹Ÿåºã
ãããã®åŸã0.4mMã®ã«ã«ã»ã€ã³æ°Žæº¶æ¶²ïŒmlã§
æ°Žåãããã«ããã¯ã¹æ³ã«ãŠç²Ÿè£œåµé»ã¬ã·ãã³å
ã³ã³ã¬ã¹ãããŒã«ã®çµæ¿åºŠïŒãã€ã¯ãã¢ã«ãšãªã
ãªããœãŒã ã調補ããã次ãã§ãã»ãã¢ãŒã¯ã¹ïŒ§
â75ã«ã©ã ã«ãŠããªããœãŒã å æ°Žå±€ã«æªå°å ¥ã®ã«
ã«ã»ã€ã³ãé€å»ãããåŸãããã«ã«ã»ã€ã³å°å ¥ãª
ããœãŒã ãžã®ãã«ããã€ã«åãã¢ã«ãã³é žè¢«èŠ
ã¯ããã«ããã€ã«åãã¢ã«ãã³é žèªå°äœãç²æ«ã®
ãŸãŸãªããœãŒã ãžçµæ¿åºŠïŒãïŒmgïŒmlãšãªããã
æ·»å ããçŽïŒæéç·©åã«æ¹æããããã¹ããªããŒ
ãŒïŒ€ã«å¯Ÿããæµææ§ã®è©Šéšã¯ããªããœãŒã è©ŠæïŒ
mlã«å¯Ÿãããã¹ããªããŒãŒïŒ€ïŒæ±æŽéžé (æ ª)補ïŒ23
åäœãæ·»å ãããªããœãŒã å æ°Žå±€ããæŸåºããŠã
ã氎溶æ§è¢å è²çŽ ã«ã«ã»ã€ã³ã®è¢å ãå±èµ·æ³¢é·
480nmãè¢å æ³¢é·510nmã«ãŠçµæçã«æž¬å®ããã
ãªããå æ°Žå±€ã§å°å ¥ãããŠããã«ã«ã»ã€ã³ã®ç·é
ã¯ã10ïŒ TritonXâ100ã®0.05mlãå ãããªããœ
ãŒã ãç Žå£ããããšã§æ±ããããã®çµæãè¡šâïŒ
ã«ç€ºãããã®å Žåããã«ããã€ã«åãã¢ã«ãã³é ž
èªå°äœãšããŠãHAââNAïŒHA5ââNA
åã³HA2ââNAãçšããããŸããå¯Ÿç §ãšããŠ
被èŠããªããªããœãŒã ãè©Šéšã«äŸãããè¡šâïŒã«
瀺ããçµæãããããããã«ã被èŠããªããªããœ
ãŒã ã«ãããŠãå æ°Žå±€äžã§æ¿åºŠæ¶å ããŠããã«ã«
ã»ã€ã³ã¯ãå šå°å ¥éã®90ïŒ è¿ããŸã§æŸåºãããã
ããã«å¯Ÿãããã«ããã€ã«åãã¢ã«ãã³é žèªå°äœ
被èŠãªããœãŒã ã§ã¯20ã30ïŒ ãŸã§ããæŸåºãããª
ãã€ãããã¹ããªããŒãŒïŒ€ã¯ãã¬ã·ãã³ã®ã³ãªã³
ãšã¹ãã«ãå æ°Žå解ãããªããœãŒã ã®äºååèæ§
é ã«ä¹±ããçãããããã®ã§ããã®çµæãå æ°Žå±€
å°å ¥ç©ãæŸåºããŠãããã®ã§ããããã€ãŠããã«
ããã€ã«åãã¢ã«ãã³é žã¯ããªããœãŒã ã被èŠã
ãããšã§ãã¹ããªããŒãŒïŒ€æµææ§ãæ®çºãããªã
ãœãŒã èæ§é ã®å®å®åããããããã®ã§ããã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ äžèšäžè¬åŒïŒïŒåã³äžè¬åŒïŒïŒã§è¡šãã
ãããã¢ã«ãã³é žèªå°äœã®äžããéžã°ããä¹³åå®
å®å€ã ãåã ãåã ïŒåèšåŒäžãR1ã¯ã¢ã·ã«åºãæå³ããR2ã¯ã
ãããã·ã«åºãâOMåºïŒåŒäžãïŒã¯ã¢ã«ã«ãªé
å±ååãæå³ããïŒåã¯âNH2åºãæå³ããã
ããŠïœã¯ïŒä»¥äžã®æŽæ°ãæå³ããïŒ
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1277039A JPH03143540A (ja) | 1989-10-26 | 1989-10-26 | æ°èŠãªä¹³åå®å®å€ |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1277039A JPH03143540A (ja) | 1989-10-26 | 1989-10-26 | æ°èŠãªä¹³åå®å®å€ |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH03143540A JPH03143540A (ja) | 1991-06-19 |
JPH0577450B2 true JPH0577450B2 (ja) | 1993-10-26 |
Family
ID=17577931
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1277039A Granted JPH03143540A (ja) | 1989-10-26 | 1989-10-26 | æ°èŠãªä¹³åå®å®å€ |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH03143540A (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3563440B2 (ja) * | 1994-05-16 | 2004-09-08 | çååŠå·¥æ¥æ ªåŒäŒç€Ÿ | ã¢ã·ã«åãã¢ã«ãã³é žã®è£œé æ³ |
JP3556975B2 (ja) * | 1994-08-11 | 2004-08-25 | æ ªåŒäŒç€Ÿè³çå | ã¢ã»ãã«åãã¢ã«ãã³é žã®è£œé æ¹æ³åã³ç²Ÿè£œæ¹æ³ |
WO1996005233A1 (fr) * | 1994-08-11 | 1996-02-22 | Shiseido Co., Ltd. | Acide hyaluronique acetyle a faible poids moleculaire, emollient, et procedes d'obtention et de purification de cet acide |
AU740111B2 (en) | 1996-09-27 | 2001-11-01 | Jagotec Ag | Hyaluronic drug delivery system |
EP1110971B1 (en) * | 1999-12-14 | 2004-08-11 | Kibun Food Chemifa Co., Ltd. | Propylene glycol hyaluronate and agent for external use to skin using the same |
US6710038B1 (en) | 1999-12-14 | 2004-03-23 | Kibun Food Chemifa Co., Ltd. | Emulsification method using propylene glycol hyaluronate |
JP2003012445A (ja) * | 2001-06-26 | 2003-01-15 | Kanebo Ltd | å粧æ |
US7179530B2 (en) | 2001-12-03 | 2007-02-20 | Rebac Gmbh | Antimicrobial composite material |
JP2005239687A (ja) * | 2004-02-27 | 2005-09-08 | Nobuhiko Yui | å¢èå æäžè¬ |
WO2008150832A1 (en) * | 2007-05-31 | 2008-12-11 | Sanofi-Aventis | Assay methods for identifying agents that modify the activity of nape-pld or abh4 |
JP5530400B2 (ja) * | 2011-05-24 | 2014-06-25 | ããŠãŒããŒæ ªåŒäŒç€Ÿ | ä¹³åå€ |
WO2013031313A1 (ja) * | 2011-09-01 | 2013-03-07 | æ ªåŒäŒç€Ÿ è³çå | ãã¢ã«ã©ãŒååŠççšçµæç© |
-
1989
- 1989-10-26 JP JP1277039A patent/JPH03143540A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
JPH03143540A (ja) | 1991-06-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2006294207B2 (en) | Aryl/alkyl succinic anhydride hyaluronan derivatives | |
JPH0577450B2 (ja) | ||
Mukhopadhyay et al. | Structure and dynamics of a molecular hydrogel derived from a tripodal cholamide | |
Dey et al. | Impact of organic polar solvents (DMSO and DMF) on the micellization and related behavior of an anionic (AOT), cationic (CEM2AB) and cationic gemini surfactant (16-5-16) | |
Laurent et al. | Synthesis and surface-active properties of uronic amide derivatives, surfactants from renewable organic raw materials | |
Zhi et al. | Synthesis, adsorption and aggregation properties of new saccharide-cationic surfactants | |
CN110997069B (zh) | äœäžºä¹³ååååæ£å©åçéŒ æç³èè¡çç© | |
Gabdrakhmanov et al. | Supramolecular design of biocompatible nanocontainers based on amphiphilic derivatives of a natural compound isosteviol | |
JPH069707A (ja) | é«ã¢ã»ãã«åçãã¢ã«ãã³é žåã³ãã®è£œé æ¹æ³ | |
JP2020041146A (ja) | çé¢æŽ»æ§å€çµæç© | |
Li et al. | Micellization of glucose-based surfactants with different counter ions and their interaction with DNA | |
Halasová et al. | Hyaluronan-surfactant interactions in physiological solution studied by tensiometry and fluorescence probe techniques | |
JPH0755961B2 (ja) | æ°èŠãªãã¢ã«ãã³é žèªå°äœåã³ãã®è£œé æ¹æ³ | |
Zhang et al. | Micellization of lactosylammonium surfactants with different counter ions and their interaction with DNA | |
Pérez et al. | A synthetic alternative to natural lecithins with antimicrobial properties | |
JP2000143704A (ja) | ãããªãªãŽç³èªå°äœ | |
Zhong et al. | Phycocyanin-chitosan complex stabilized emulsion: preparation, characteristics, digestibility, and stability | |
CN116536379A (zh) | äžç§æµ·è»ç³èèªé žåé ¯åäºé ¯çå¶å€æ¹æ³åå ¶åºçš | |
Lakhrissi et al. | Surface and selfâaggregation properties of bisâbenzimidazolones derivatives of Dâglucose | |
Lin et al. | Synthesis and cytotoxicity of quercetin/hyaluronic acid containing ether block segment | |
VÄrÄÅteanu et al. | Aspects regarding the synthesis and surface properties of some glycine based surfactants | |
JPH09136896A (ja) | ã¢ã·ã«åãããã·ãŠã¯ããŒã¹ã¢ãã«ã«ãã³é žé¡ | |
JP2713359B2 (ja) | ç³è質åçé¢æŽ»æ§å€ | |
TWI708636B (zh) | 幟äžèé£-èºåºé žåçé¢æŽ»æ§åãå ¶è£œåæ¹æ³èæçš | |
JP3217450B2 (ja) | ãªã³è質å°èäœçšå®å®åå€ |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |