JPH0577028B2 - - Google Patents
Info
- Publication number
- JPH0577028B2 JPH0577028B2 JP27611885A JP27611885A JPH0577028B2 JP H0577028 B2 JPH0577028 B2 JP H0577028B2 JP 27611885 A JP27611885 A JP 27611885A JP 27611885 A JP27611885 A JP 27611885A JP H0577028 B2 JPH0577028 B2 JP H0577028B2
- Authority
- JP
- Japan
- Prior art keywords
- cell
- hematoporphyrin
- dna
- piperazyl
- benzimidazolyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 239000003153 chemical reaction reagent Substances 0.000 claims description 23
- UJKPHYRXOLRVJJ-MLSVHJFASA-N CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C Chemical compound CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C UJKPHYRXOLRVJJ-MLSVHJFASA-N 0.000 claims description 22
- 229960003569 hematoporphyrin Drugs 0.000 claims description 22
- 238000010186 staining Methods 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 16
- 239000012192 staining solution Substances 0.000 claims description 16
- 239000007850 fluorescent dye Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 238000005259 measurement Methods 0.000 claims description 5
- UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 claims description 4
- 229960003151 mercaptamine Drugs 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 4
- 238000000691 measurement method Methods 0.000 claims 2
- 235000002639 sodium chloride Nutrition 0.000 claims 2
- 239000004094 surface-active agent Substances 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 239000012752 auxiliary agent Substances 0.000 claims 1
- 125000003785 benzimidazolyl group Chemical group N1=C(NC2=C1C=CC=C2)* 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 59
- 108020004414 DNA Proteins 0.000 description 30
- 102000053602 DNA Human genes 0.000 description 30
- 206010028980 Neoplasm Diseases 0.000 description 19
- 201000011510 cancer Diseases 0.000 description 19
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 13
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 10
- 210000000805 cytoplasm Anatomy 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 7
- 210000004698 lymphocyte Anatomy 0.000 description 7
- 210000004940 nucleus Anatomy 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 230000029087 digestion Effects 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000116 DAPI staining Methods 0.000 description 3
- 208000002495 Uterine Neoplasms Diseases 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000012757 fluorescence staining Methods 0.000 description 3
- 206010046766 uterine cancer Diseases 0.000 description 3
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 238000010867 Hoechst staining Methods 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 230000006727 cell loss Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000000093 cytochemical effect Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005562 fading Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 230000013777 protein digestion Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 2
- HYZJCKYKOHLVJF-UHFFFAOYSA-N 1H-benzimidazole Chemical compound C1=CC=C2NC=NC2=C1 HYZJCKYKOHLVJF-UHFFFAOYSA-N 0.000 description 1
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000000224 granular leucocyte Anatomy 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- -1 phenyl indole compound Chemical class 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27611885A JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27611885A JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62135769A JPS62135769A (ja) | 1987-06-18 |
| JPH0577028B2 true JPH0577028B2 (de) | 1993-10-25 |
Family
ID=17565043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27611885A Granted JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62135769A (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0782009B2 (ja) * | 1988-03-24 | 1995-09-06 | 工業技術院長 | 細胞融合方法及び装置 |
| JPH02168161A (ja) * | 1988-12-22 | 1990-06-28 | Nikon Corp | 細胞の良・悪性判定装置 |
| JP2688002B2 (ja) * | 1989-10-03 | 1997-12-08 | 浜松ホトニクス 株式会社 | 細胞内遺伝情報消去方法およびその装置 |
| US6001573A (en) * | 1996-06-14 | 1999-12-14 | Packard Bioscience B.V. | Use of porphyrins as a universal label |
| EP0812920A1 (de) * | 1996-06-14 | 1997-12-17 | Packard Instrument B.V. | Verwendung von Porphyrinen bei einem Verfahren zur Instrumental-Erkennung |
-
1985
- 1985-12-10 JP JP27611885A patent/JPS62135769A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62135769A (ja) | 1987-06-18 |
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