JPH05244952A - リボ核酸の特異的生産方法 - Google Patents

リボ核酸の特異的生産方法

Info

Publication number
JPH05244952A
JPH05244952A JP4254548A JP25454892A JPH05244952A JP H05244952 A JPH05244952 A JP H05244952A JP 4254548 A JP4254548 A JP 4254548A JP 25454892 A JP25454892 A JP 25454892A JP H05244952 A JPH05244952 A JP H05244952A
Authority
JP
Japan
Prior art keywords
template
nucleic acid
promoter
oligonucleotide
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4254548A
Other languages
English (en)
Japanese (ja)
Inventor
Udo Reischl
ウード・ライシュル
Ruediger Rueger
リューディガー・リューガー
Christoph Kessler
クリストフ・ケスラー
Rudolf Seibl
ルドルフ・ザイブル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JPH05244952A publication Critical patent/JPH05244952A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP4254548A 1991-09-26 1992-09-24 リボ核酸の特異的生産方法 Pending JPH05244952A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4132133-2 1991-09-26
DE4132133A DE4132133A1 (de) 1991-09-26 1991-09-26 Verfahren zur spezifischen herstellung von ribonukleinsaeuren

Publications (1)

Publication Number Publication Date
JPH05244952A true JPH05244952A (ja) 1993-09-24

Family

ID=6441593

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4254548A Pending JPH05244952A (ja) 1991-09-26 1992-09-24 リボ核酸の特異的生産方法

Country Status (5)

Country Link
US (1) US5369003A (enExample)
EP (1) EP0534345A2 (enExample)
JP (1) JPH05244952A (enExample)
CA (1) CA2078906A1 (enExample)
DE (1) DE4132133A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837453A (en) * 1992-05-13 1998-11-17 Geron Corporation Telomerase activity assays
US6200753B1 (en) * 1993-06-03 2001-03-13 Intelligene Ltd. Detection of nucleic acid sequences
US5871914A (en) * 1993-06-03 1999-02-16 Intelligene Ltd. Method for detecting a nucleic acid involving the production of a triggering RNA and transcription amplification
IL105894A (en) * 1993-06-03 1997-02-18 Asher Nathan Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
US5804380A (en) * 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
US5863726A (en) * 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
IL111857A (en) * 1994-12-02 1999-09-22 Intelligene Ltd Detection of nucleic acid sequences
JPH11507839A (ja) * 1995-06-07 1999-07-13 ジェロン コーポレイション テロメラーゼ活性検定
US5914229A (en) * 1996-06-14 1999-06-22 Sarnoff Corporation Method for amplifying a polynucleotide
US20050136411A1 (en) * 2003-12-22 2005-06-23 Collins Patrick J. Methods and compositions for linear mRNA amplification from small RNA samples

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0427074A2 (en) * 1989-11-09 1991-05-15 Miles Inc. Nucleic acid amplification employing transcribable hairpin probe

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
WO1989001050A1 (en) * 1987-07-31 1989-02-09 The Board Of Trustees Of The Leland Stanford Junior University Selective amplification of target polynucleotide sequences
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
HU216105B (hu) * 1988-12-16 1999-04-28 Akzo Nobel N.V. Amplifikációs eljárás önfenntartó szekvenciareplikációs rendszerrel
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0427074A2 (en) * 1989-11-09 1991-05-15 Miles Inc. Nucleic acid amplification employing transcribable hairpin probe

Also Published As

Publication number Publication date
DE4132133A1 (de) 1993-04-01
EP0534345A3 (enExample) 1995-05-24
EP0534345A2 (de) 1993-03-31
US5369003A (en) 1994-11-29
CA2078906A1 (en) 1993-03-27

Similar Documents

Publication Publication Date Title
JP2522888B2 (ja) 核酸配列の特異的増幅方法
JP3644976B2 (ja) 核酸の増幅の改良法
EP0519338B1 (en) Improved methods for nucleic acid amplification
US5270184A (en) Nucleic acid target generation
JP2807202B2 (ja) 核酸の高感度検出方法
US6063604A (en) Target nucleic acid sequence amplification
JP3936798B2 (ja) Ｒｎａ標的配列の増幅方法
JP2846018B2 (ja) 核酸配列の増幅および検出
US5112734A (en) Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
US6030774A (en) Method for introducing defined sequences at the 3' end of polynucleotides
US6090556A (en) Method for quantitatively determining the expression of a gene
US5627054A (en) Competitor primer asymmetric polymerase chain reaction
US6100024A (en) Methods and compositions for nucleic acid detection by target extension and probe amplification
JPH048293A (ja) ライゲーシヨン可能なヘアピンプローブ及びその転写を用いた核酸の増巾方法
JP3909010B2 (ja) 高度ダイナミックレンジを有する定量的多重ｐｃｒ
EP2491146A1 (en) Amplification primers with non-standard bases for increased reaction specificity
JPH05244952A (ja) リボ核酸の特異的生産方法
WO2004050911A2 (en) Method for lowering both sequence variations and increase of base lines effects in diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
JPH04211400A (ja) 修飾核酸の製造方法
JPH1066589A (ja) ホウ素化ヌクレオチドを用いた鎖置換増幅
WO1994017206A1 (en) Method for amplifying nucleic acid sequences
JPH06327500A (ja) 核酸配列の増幅方法および検出方法
US6001610A (en) Method for the particularly sensitive detection of nucleic acids
JPH05211873A (ja) 核酸鋳型の特異的増殖方法および核酸の決定方法
EP0791075A2 (en) Use of spermidine to relieve inhibition of ligase chain reaction in a clinical test sample