JPH05229956A - Antioxidant - Google Patents

Abstract

PURPOSE:To obtain an antioxidant containing an extract of Carthamus Tinctorius L. with an solvent, excellent in safety and further having action capable of inhibiting activity of hyaluronidase. CONSTITUTION:The objective antioxidant contains an extract obtained by extracting Carthamus Tinctorius L. which is a plant belonging to Dicotyledoneae, Sympetalae, Campanulales, Compositae, Carthamus with a hydrophilic organic solvent (e.g. ethanol) as an active ingredient. The extract of Carthamus Tinctorius L. has strong antioxidant action and is a natural product and has been for long years used as an emmenagogue and an blood cleaning agent in treatment for women's disease, oversensitive condition to cold, climacteric disturbance, etc., and has high safety to human. The agent retains lubricating property and softness of skin and has effect capable of suppressing decomposition of hyaluronic acid preventing outer force and bacterial cell.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antioxidant, and further relates to an antioxidant effective in inhibiting the activity of hyaluronidase.

[0002]

2. Description of the Related Art Vegetation is a flower of dicotyledonous plant, joint-venture sub-net, Cryptera, and the scientific name of the family Asteraceae is Carthamus Tinctorius L. safflower or Klenoai. Safflower is used as a blood purifying drug for the treatment of women's diseases, chills, and menopause. It has also been used as a pigment for lipsticks since ancient times.

At present, the antioxidants mainly used are butylhydroxyanthony (BHA), butylhydroxytoluene (BHT), vitamin E and its derivatives. BHA and BHT are synthetic products, and as a general common sense nowadays, the use of synthetic products is subject to severe criticism because long-term safety is not guaranteed. It's starting. Also vitamin E
Synthetic products and derivatives thereof are included in natural products such as rice bran and have sufficient safety, but substances having a relatively weak antioxidant action and having a strong oxidizing action are desired.

[0004]

The object of the present invention is to have a strong antioxidative effect, and it has been confirmed that it is a natural product and has been used internally for many years in the human body, and its safety to the human body has been confirmed. It is to provide an antioxidant that exerts effects other than the above.

[0005]

Means for Solving the Problems As a result of screening the substances for solving the above problems from natural substances such as plants, animals and foods, the present inventors have found that the solvent extract of safflower has a strong antioxidant action. The present invention was completed by finding out that they have.

That is, the present invention is an antioxidant containing a solvent extract of safflower as an active ingredient.

[0007] Safflower grows naturally in Japan, is easily available, and is marketed as a raw material for pharmaceuticals. The safflower is extracted with a hydrophilic organic solvent, and as the solvent, generally ethanol, methanol, butanol, acetone or a mixture thereof can be used. It is also possible to replace part of it with water. Also,
Polyhydric alcohols such as 1,3 butylene glycol and glycerin can also be used. Judging from the effects of hyaluronidase activity inhibition and antioxidant, extraction with an aqueous solution of about 50% ethanol was appropriate.

The antioxidant of the present invention can also be used in combination with other antioxidants, such as vitamin E, the conchiolin hydrolyzate of Japanese Patent Application No. 2-138457 by the present applicant, and the Japanese Patent Application. It can also be used in combination with the extract from cashew nuts of JP-A-3-217484. There is no particular limitation on the target compounded as an antioxidant, and cosmetics and the like are effective because they also have an antioxidant effect after application, that is, this antioxidant effect is effective not only in the preparation but also after application to the skin. Therefore, it also has an antioxidant effect on sebum. Further, the dosage form of the cosmetic is not particularly limited, and it can be blended in creams, lotions, packs and the like.

[0009]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

In the examples, production examples of the antioxidant of the present invention used will be shown. (Production Example 1) 200 g of ethanol was added to 10 g of safflower and left for 5 days. This was filtered with No5C filter paper and freeze-dried.

(Production Example 2) 200 g of 50% ethanol was added to 10 g of safflower and left for 5 days. This was filtered with No5C filter paper and freeze-dried.

(Example 1) Lotion Olive oil 0.5 Ethanol extract of safflower of Production Example 1 0.5 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) Hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

Example 2 Cream A Squalane 20.0 Olive Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerin Monostearate 1.0 Sorbitan Monostearate 2 .0 50% ethanol extract of safflower of Production Example 1 1.0 B Purified water 47.9 Polyoxyethylene (20 E.O.) sorbitan monostearate 2.0 Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 Glycerin 5.0 1.0% sodium hyaluronate aqueous solution 5.0 Methyl paraoxybenzoate 0.1 Weigh A and B respectively, heat to 70 ° C., and gradually add A to B with stirring After that, while stirring slowly, 30
Cooled to ° C.

(Antioxidant test) The following test solutions were prepared in 50 ml test tubes with screw caps. Specimen 5 mg 1% ethanolic linoleic acid solution 10 ml 0.1 M, pH 7.0 phosphate buffer 10 ml purified water 5 ml This is left in a constant temperature bath at 40 ° C while being shielded from light. The following measurements were taken before 4 days, 8 days, 12 days, and 17 days before putting this in a constant temperature bath. 0.125 ml of test solution, 12.125 ml of 75% ethanol, and 0.125 ml of 30% ammonium thiocyanate were added, stirred and left for 3 minutes, then 0.02N
0.125 ml of 3.5% ferric chloride aqueous solution of HC1 was added, and the mixture was stirred and allowed to stand for 3 minutes, and then the absorbance was measured at a wavelength of 500 nm. The cell length was 10 mm, and the control cell was a blank in which the test solution was replaced with water. (The same sample was measured three times and averaged) The measurement results are shown in Table 1 together with the results of the water of the control example, Vitamin E of Comparative Example 1, and BHT of Comparative Example 2.

[0015]

[Table 1]

(Hyaluronidase activity inhibition test) Hyaluronidase is widely distributed in the living body and is also present in the skin.
As its name implies, it decomposes hyaluronic acid. Hyaluronic acid is a linear polymeric polysaccharide in which β-D-N-acetylglucosamine and β-D-glucuronic acid are alternately linked, and is a glycosaminoglycan that is widely present in connective tissues of mammals along with chondroitin sulfate. It is a kind. As a function of hyaluronic acid in connective tissue, it retains water in the intercellular spaces, forms a jelly-like matrix in the tissue to retain cells, keeps skin's lubricity and flexibility, and exerts external force (mechanical force). Injury) and bacterial infections. Therefore, suppressing the activity of this hyaluronidase suppresses the degradation of hyaluronic acid, the stability of hyaluronic acid during the use of this formulation, the stability of hyaluronic acid in the formulation after application to the skin, and the hyaluronic acid present in the skin. It is also considered to contribute to the stability of the acid itself.

(Test Method) 6 g of 0.4% sodium hyaluronate 0.1M (pH 6.0) phosphate buffer solution was weighed and left in a 37 ° C. constant temperature water bath for 5 minutes. 0.1 wt / v% aqueous solution (if it is difficult to dissolve, add ethanol to dissolve it, then add purified water and evaporate to remove ethanol.
1.0 ml (adjusted to be 1 wt / v%) was added and stirred, and 0.01% hyaluronidase (manufactured by Sigma, beef testis, type I-S) 0.1M (pH 6.0) phosphate buffer solution was added. 1 ml was added and immediately stirred, and 6 ml was placed in an Ostwald viscometer placed in a constant temperature water bath at 37 ° C. The viscosity was measured after 1 minute, 5 minutes, 10 minutes, 20 minutes, and 40 minutes. Viscosity after 1 minute is set to 100 and displayed as an index. As a control, pure water was added instead of the sample solution and the same measurement was performed. The final concentration of the sample in this test is 0.0125
%. The results of this measurement are shown in Table 2.

[0018]

[Table 2] From these results, it can be seen that the product extracted with the 50% ethanol aqueous solution of Production Example 2 has a particularly large effect of suppressing hyaluronidase activity.

(Panel test) Eight women were asked to continuously use the embodiment and comparative example for one month using their right and left faces. Lotions and creams were used in combination in both Examples and Comparative Examples. The comparative example is obtained by removing the manufacturing example from the example. The results are shown in Table 3. 1 = Comparative example is better 4 = Example is better 2 = Comparative example is better 5 = Example is better 3 = No difference

[0020]

[Table 3]

[0021]

EFFECT OF THE INVENTION The antioxidant of the present invention has a strong antioxidant action, is a natural product, and has been applied to the human body for many years for internal use, and its safety to the human body has been confirmed. Although there is a slight difference depending on the extraction solvent, a hyaluronidase activity suppressing effect is recognized in addition to the antioxidant effect. When added to cosmetics and the like, it contributes to the antioxidant of sebum and the stability of hyaluronic acid present in the skin, and exhibits excellent effects.

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Claims (1)

[Claims] 1. An antioxidant comprising a safflower solvent extract as an active ingredient.