JPH05130839A - Feed for silkworm and method for breeding - Google Patents

Abstract

PURPOSE:To obtain a feed for silkworm capable of breeding silkworms inexpensively, efficiently and constantly throughout yarn without using mulberry leaves, comprising a mixture of specific chlorophyll and a specific polysaccharide. CONSTITUTION:The objective feed for silkworm comprising a mixture of chlorophyll obtained by extraction of plant leaves such as cherry leaves, rice leaves, tea leaves or mulberry leaves or chlorophyll on the market and a polysaccharide obtained by extracting embryo buds, soybean protein or powder of marine alga such as sea mustard besides plant leaves with an organic acid (e.g. malic acid). The feed is preferably mixed with a chlorophyll stabilizer composed of a substance prepared by reacting chlorophyll with vitamin C or a solution having dissolved the chlorophyll in water or an organic solvent is directly sprayed upon silkworms or plant leaves to breed silkworms.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a silkworm artificial rearing feed and a rearing method for replacing mulberry leaf which is a natural feed.

[0002]

2. Description of the Related Art Due to the industrial problem of cocoon production, artificial feeding has been considered in place of the traditional live mulberry leaf breeding. As a result, breeding costs, breeding space, etc. (1-5th) Artificial breeding is difficult, and a production method was devised in which the larvae (1st-3rd) were fed with artificial feeds, and when they were in the silkworm period, they were fed with raw mulberry leaves to take cocoons. It has been put to practical use. According to this method, the feed cost, the breeding space are small, and the labor saving is achieved.

However, the cocoons obtained by this method are disadvantageous in that they are slightly smaller than those cultivated by mulberry, and this artificial feed contains about 20 to 30% of mulberry powder (raw mulberry leaves). It contains dried and crushed ones, hereinafter referred to as MLP). Without this MLP, smooth growth cannot be achieved, and the resulting cocoons are small. Furthermore, this MLP has the highest unit price among feed ingredients, which is one of the obstacles to the production cost. The greatest concern is to eliminate this MLP and to achieve sufficient growth, and many studies have been made so far, but this problem could not be solved.

On the other hand, studies have also been conducted on active ingredients of mulberry leaves, such as cellulose, sugar, inositol, β-sitosterol, isoquercitrin, potassium phosphate, morin, chlorogenic acid, choline, gallic acid and oleic acid. The ingredients have been removed. Such components are not peculiar to mulberry leaves, but are universally present in the plant kingdom. However, with a feed containing only these components, the growth of silkworms is insufficient, and satisfactory growth is possible only by mixing MLP. From these facts, it can be seen that mulberry leaves and MLPs still contain unknown components that are important for the growth of silkworms.

[0005]

[Problems to be Solved by the Invention] MLP contains components essential for the growth of silkworms, and it is important to elucidate the components and apply them to create good feed.
In the current feed using MLP, it takes a lot of time and effort to produce MLP from mulberry leaves, and it has the highest unit price among artificial feed materials. Therefore, ML
If the active ingredient in place of P can be isolated, the breeding performance will be improved and an inexpensive feed can be supplied. Further, mulberry leaves also contain a growth inhibitor, and in the case of mulberry, it is said to be hemicellulose, but it is expected that these substances will also be removed in accordance with the pursuit of active ingredients.

[0006] Mulberry leaves have "incentives" and "bites" for silkworms
There are three factors that cause actions such as "swallow", and these are each of the above components, and the methanol extract of mulberry leaves has been considered effective for a long time, but this fraction was further extracted with ether. Β-sitosterol was taken out of the plant and designated as a "bite" factor. This judgment is because when silkworm soaked β-sitosterol into agar, the silkworm acted as a “bite”, but this judgment confused the progress of subsequent research, and β-sitosterol was added to artificial feed. However, the action of "biting" did not occur, and the action of "biting" occurred by inserting MLP in it. β-sitosterol had only the nutritional effect of sterol.

The inventors of the present invention first of all used MLP instead of mulberry leaves.
Starting with the examination of a methanol extract that is considered to be effective using the above as the raw material, it was found that there are silkworm growth promoting components other than mulberry leaves, and the silkworm feed containing the growth promoting substance as a main component. And a breeding method.

[0008]

In order to achieve the above object, the silkworm feed of the present invention comprises chlorophyll obtained by extracting from plant leaves such as cherry leaves, rice leaves, tea leaves, mulberry leaves, or algae, or commercially available chlorophyll. It is characterized by comprising a mixture of chlorophyll and a polysaccharide obtained by extracting and processing plant leaves, germs, soybean powder, seaweed powder such as seaweed and the like with an organic acid. It should be noted that it is preferable to add to this feed, as a chlorophyll stabilizer, a reaction product of chlorophyll such as vitamin C, polyphenol aldehyde, polyphenolic acid, and sulfur-containing amino acid.

The present invention is also characterized in that, as a method for rearing silkworms, a solution prepared by dissolving the above chlorophyll in water or an organic solvent is directly sprayed on silkworms or mulberry leaves.

[0010]

[Function] It was found that chlorophyll, a dark oily substance extracted from plant leaves or algae, is a component that promotes the growth of silkworms. Furthermore, this chlorophyll contains plant leaves, germs, soybean powder, seaweed powder such as wakame, etc. It was found that mixing polysaccharides obtained by extraction treatment with acid produces a synergistic effect on silkworms.

Chlorophyll is contained in many plants, algae, etc., and it is possible to easily extract it by selecting a cost-effective one from the raw materials thereof, and to obtain a polysaccharide in the same manner. Is. Since chlorophyll can also be stored, it is cheaper than mulberry powder and can reduce feed costs. In addition, by using an appropriate amount and mixing of chlorophyll stabilizing substances, efficient breeding can be performed regardless of the season, and large cocoons can be obtained, which can change the production system of cocoons and raw silk. It is a thing.

[0012]

EXAMPLES Next, the examples of the present invention will be described in detail. First, MLP was used as a raw material instead of mulberry leaves, and water was added to a methanol extract which was considered to be effective.
After -80%, the precipitate was redissolved in a solvent such as petroleum ether and the solvent removed to give a dark oil.

When the absorption spectrum of this oily substance was measured, it was found that it was very similar to the known spectrum of chlorophyll a, and paper chromatography (hereinafter referred to as PCG) of the oily substance was used as known data. When the color and the Rf value were examined by comparison, it was found that in addition to chlorophyll a, chlorophyll b, and pheophoitin a, carotene was contained.

Since carotene removed by the dioxane method is also effective as a feed, carotene may be removed from the active substance. Further, as a result of the breeding test, since addition of magnesium salt is effective, pheophytin can also be removed, and among the above oily substances, chlorophyll a and chlorophyll b were found to be effective substances as feed.

Further, commercially available chlorophyll oil (manufactured by Nacalai Tesque KK, No. 086-18) has different peculiar odor due to different manufacturing raw materials, but it has a characteristic maximum absorption spectrum of chlorophyll, and PCG also shows chlorophyll a and Even when it was fed for breeding, showing the presence of phoeophytin a, it was eaten well without evading and showed good growth.

Not only mulberry leaves but also dark oily substances obtained by treating the ordinary leaves such as cherry blossoms, Japanese tea, rice, etc. in the same manner as above show breeding results almost similar to those of mulberry leaves, and their absorption spectra also. The characteristic chlorophyll spectrum is shown. Therefore, it was revealed that chlorophyll promotes silkworm growth even if the raw material is changed, and that chlorophyll a and chlorophyll b are effective. Hereinafter, the dark oily substance is referred to as chlorophyll (CHL).

Breeding of silkworms was carried out as follows. That is, various CHLs to be tested are mixed with the basic feed to be described later, water is added and kneaded into the petri dish, the petri dish is sterilized, and then washed separately with 70% alcohol, then at 25 ° C. For 10 days, the hatched silkworms were swept onto the bait and left in a constant temperature bath at 28 ° C., and after 1 week, the silkworms were taken out and the weight of the silkworms was measured.

When silkworms are raised by this method, their weight is not yet sufficient, and they are smaller than those of mulberry leaves. Then, as a result of various studies, it was found that another important component was required in addition to CHL. Then, the component was taken out from MLP as follows.

That is, when water in which citric acid (hereinafter referred to as CA) is dissolved is added to mulberry leaves or MLP, and the mixture is extracted by heating and then filtered, a large amount of methanol is added to cause precipitation. This precipitate was mixed with basic feed and bred, but the effect was small and it did not grow much. However, it was found that when CHL was added to the basic feed together with this precipitate and raised, the breeding performance was better than that of CHL alone. That is, CHL
It was found that there is a cooperative growth effect of the silkworm between this and this precipitation, and the presence of both of them increases the growth effect.

The acid used for this acid decomposition is preferably an organic acid, and in addition to CA, malic acid, succinic acid, fumaric acid, maleic acid and the like can be used. It is not good to use a mineral acid such as hydrochloric acid or sulfuric acid. As decomposition materials, in addition to MLP, mulberry leaves, etc., general leaves such as tea leaves and cherry leaves, germs such as rice and wheat, defatted soybean powder, okara, alfalfa, chlorella, spirulina, wakame seaweed Seaweed powder such as kelp and kelp treated with an organic acid can be used, and the cooperative breeding effect with CHL can be improved.

The product produced by acid decomposition was anthrone reaction positive and showed a paste-like viscosity when water was added, so it was found to be a saccharide, and the infrared spectrum also revealed that it has a large number of OH groups. As a result of the breeding test, glucose, maltose, sucrose, lactose and the like having small molecular weights have no growth effect, and dextrin and starch have no synergistic growth effect. At this time, only the degradation product of MLP used as a subject showed the effect.

As a result of measuring the approximate molecular weight by gel filtration, defatted soybean powder, okara, germ, seaweed powder,
It was revealed that the saccharides obtained from alfalfa, MLP and the like are all polysaccharides having a higher molecular weight than maltose and glucose. Hereinafter, this precipitate is referred to as a polysaccharide. The fact that silkworms have a co-breding effect between CHL and polysaccharides is a fact not known until now.

As can be seen from the fact that CHL plays a major role in photosynthesis, CHL is sensitive to light and is liable to undergo various chemical changes, and dilute acid treatment removes the central magnesium and replaces it with hydrogen. Changes to pheophytin. Also, it is decomposed by alkali to generate phytol, and the pigment body becomes chlorophyllin. Pheophytin and chlorophyllin are further decomposed into various compounds having a porphyrin nucleus.

Not only the above chemical changes due to acids and alkalis, but also subtle changes due to changes in pH. That is, the change in the absorption spectrum due to the change in pH is stable in neutral to weakly alkaline, but the position of the maximum absorption spectrum and the change in absorbance are strongly alkaline (pH 10.
Not only does it increase to 0) and the maximum decreases, but there are some places where the maximum itself disappears.

In PCG, a large change in color appears, and as a result of the experiment, the one obtained by using a buffer solution having a pH of 7.4 to 10.04 is stable in both color and Rf value, but is completely different on the acidic side. The green color of CHL almost disappeared, and the gray color of pheophytin was conspicuous. Thus, due to the change in pH, C
A change in the internal structure of HL is shown, indicating that CHL is unstable. Therefore, in order to show the constant physiological action of the feed, it is desirable that the silkworm is in a stable state even when it enters the body, and in order to fully exert the constant physiological activity of the feed, it is necessary to stabilize CHL. Is desired.

Then, as a result of various studies on the stabilization of CHL, it was found that vitamin C, polyphenol aldehyde, polyphenolic acid, sulfur-containing compounds, especially sulfur-containing amino acids have a stabilizing effect. CHL like this
When the substance to be stabilized is soluble in methanol, the substance is dissolved in an extract containing CHL, and then water is added to precipitate CHL. If is soluble in water, dissolve in water,
The aqueous solution was added to a methanol extract containing CHL to obtain C
HL was deposited. After that, as described above, it was taken out after being dissolved in a solvent such as petroleum ether.

Stabilization of CHL with vitamin C was performed as follows. Since vitamin C has strong acidity, a sodium salt (hereinafter referred to as VC-Na) having the same effect was used, but a fatty acid ester can also be used. This VC
-Na was dissolved in water and a buffer solution having various pH changes, and this was added to a CHL methanol extract solution to obtain a precipitate. The absorption spectrum of each of these was measured and PCG was performed.

The absorption spectrum shows an absorption maximum at the same position as when VC-Na is not added in the case of acidity, but it becomes 430 nm when a buffer solution of pH 7.4 and pH 10.0 is added. A clear absorption maximum with a large extinction coefficient appears in
It is believed that this was caused by the addition of VC-Na. The absorption coefficient becomes higher and the existence becomes clearer when the buffer solution of strongly alkaline pH 10.0 is added. This 43
It was found that the absorption coefficient at the absorption maximum at 210 nm in the ultraviolet region also increased in parallel with the absorption maximum at 0 nm. When VC-Na is not added, the shape of the absorption curve is distorted in the case where the buffer solution of pH 10.0 is added, compared to the addition of VC-Na, which enables stable CHL and improves stability. I found out that

Further, when looking at PCG, the pheophytin appearing in gray disappeared completely, which proved that the VC-Na prevented the magnesium of CHL from separating from CHL and made it stable. When the pH 7.4 buffer is used, blue-green CHL-a and yellow-green CHL-b are clearly visible, and when the pH 10.0 buffer is used, C
The green color of HL spreads over the chromatogram. Acidity-At pH 6.0, browning of the feed can be seen when the feed containing MLP is mixed with acidic substances such as VC and CA.
The brown color appeared in the CHL-a position as it was.

As described above, when VC-Na was added, the chromatogram prevented the formation of pheophytin when compared with the case of simply adding water, and when made alkaline, green CHL was obtained.
It was found that CHL was stabilized by the addition of VC-Na. This also appears in the breeding performance,
This is also supported by the fact that the breeding performance of alkaline CHL treated with VC-Na is improved.

It was found that polyphenol aldehyde, polyphenolic acid and the like also have an action of stabilizing CHL, and the stabilization of CHL by these was performed as follows. In this case, dissolve the aldehyde or acid in a methanol extract containing CHL, and add water or
A pH buffer solution was added, the reaction was carried out at each pH, the reaction solution was extracted with petroleum ether, and then the petroleum ether was removed to take out the reaction product.

In order to find the optimum pH condition for the reaction, protocatechualdehyde was used to take out the reaction product under each pH condition, and its properties were examined. When this was observed by PCG, the one obtained by reacting by adding a buffer solution of pH 7.4 showed a stable green color, and on the acidic side than that, the spot of gray pheophytin from which magnesium was detached was the same as before. It was found that when the alkalinity became strong, it became unstable again and decomposed to produce gray fephytin. Where this point is different from the case of vitamin C, in the case of pitamin C, decomposition did not occur even if the alkalinity became strong.

Therefore, the respective aldehydes and acids are
Dissolve in a methanol solution containing CHL, and add pH 7-8.
The buffer solution of was added and reacted. This was transferred to petroleum ether, and the petroleum ether was removed to obtain a reaction product.

Looking at the absorption spectra of the respective products obtained by reacting protocatechualdehyde, protocatechuic acid and gallic acid, the absorption band around 660 nm does not change like vitamin C, but Was seen
There was no absorption band around 430 nm, and there was a clear absorption maximum around 450 and 410 nm. However, the characteristic is that the absorption maximum at around 210 nm is low, and vitamin C
In the case of, the absorbance value increases as the alkalinity increases. This is a big difference from the case of vitamin C.

Examples of polyphenolic acid and polyphenolaldehyde include cinnamic acid, chlorogenic acid, β
-, Γ-resdolcylic acid, gentisic acid, quinic acid and cinnamic acid aldehyde, gentisin aldehyde, quinic acid aldehyde, gallic aldehyde, β-, γ-resorcylic aldehyde, vanillin and the like can be used.

As a result of breeding silkworms by mixing these with the basic feed, the growth effect is equal to or higher than that of the main body CHL, and it is used in combination with CHL or CHL stabilized with vitamin C. It was found that the growth effect was even greater.

Further, CHL reacted with a sulfur-containing amino acid
It was also found to have a growth promoting action, and the reaction with sulfur-containing amino acids was performed as follows. Since cysteine is a hydrochloride, it was dissolved in water containing K ₂ HPO ₄ for neutralization, and the solution was added as it was to a methanol extract containing CHL so that the methanol concentration was 70%.

Also in the case of the buffer solution, the buffer solution was added to the neutralized solution, and the solution was added to the extract solution so as to be 70%. In the case of methionine, too, a small amount of K ₂ HPO ₄ was added, and then it was dissolved using water or a buffer solution, and the solution was added to the CHL-containing extract for precipitation. Petroleum ether was added to the reaction solution to extract a precipitate, and then petroleum ether was removed to obtain a reaction product.

The absorption spectra of these are 45
There are absorption maxima near 0, 430 and 410 nm, and the absorption maxima at 430 nm found when vitamin C was alkaline also appeared in the case of sulfur-containing amino acids. However, this absorption maximum is not seen in the case of an acid added with a buffer solution of pH 5.0 as in the case of vitamin C. This absorption maximum at 430 nm and 210n
The maximal relationship for m is not in the parallel relationship seen with vitamin C. As a result of raising silkworms by adding these to the basic feed, it was found that the growth effect was increased by using them in combination with CHL or CHL stabilized with vitamin C.

So far, studies have been carried out using mulberry leaves or MLP as a material, and it has been shown that CHL or a stabilized product thereof is effective for the growth of silkworms. It is said that silkworms other than mulberry leaves cannot be expected to grow sufficiently, and so far, they have not been artificially bred using other dried plant leaves and their dried products. CHL is a plant pigment widely distributed in the plant kingdom, and naturally, it is also present in the leaves of other plants, and it is considered that breeding is possible by using the CHL.

Therefore, as other plants, cherry leaves, rice leaves, and Japanese tea leaves were selected, and CHL was extracted from them.
Silkworms were reared by L. As a result of breeding by adding only the CHL extract to the basic feed, the results were good, and it was confirmed that CHL extracted from these plants also had the same effect as that of mulberry leaves. CH also from this result
It was possible to confirm the effectiveness of L. For cherry blossoms, the leaves of the roadside trees were sprinkled and dried. For rice, dried rice straw was sprinkled. For Japanese tea, commercially available sencha was used as it was. Extraction was carried out in the usual way by extraction with 99% methanol and hydration to 70%, and the precipitate was isolated by extraction with petroleum ether.

As described above, the fact that CHL and its stabilized product are the main body of mulberry leaves and that silkworms can be bred without using mulberry leaves or MLP is extremely beneficial. Hereinafter, specific examples of the present invention will be shown. First, the basic feed used for rearing silkworms shown in each example is shown below.

[0043]

[Table 1]

[0044]

[Table 2]

As the silkworm variety, "Kinshu x Kanewa" or "Harumine x Kanetsuki" was used. After the silkworm seeds were washed with 70% methanol, they were allowed to stand at 25 ° C for 10 days to clean up the hatched silkworms. I went to For the feed, weigh and mix the sample CHL and polysaccharides into the above basic feed, add 5.5 ml of water to 3.35 g of the mixture, knead it, put it in a petri dish, sterilize at 100 ° C, cool and then silkworm. Were swept away, allowed to stand as it was at a temperature of 28 ° C., and after 7 days, the weight of the silkworm, 3rd instar, was measured.

Example 1 Chlorophyll was extracted as a breeding material as follows. That is, 99% methanol was added to MLP, the extract was filtered to remove petiole, and water was added to the filtrate (pH at this time).
6.43), the precipitated CHL was redissolved in petroleum ether, and the petroleum ether was further removed to obtain CHL. Similarly, the polysaccharide was extracted from MLP as a raw material as follows. That is, 200 ml of an aqueous acid solution was mixed with 20 g of MLP, heated at 100 ° C. for 1 hour, water was added to filter the whole amount, and then 150% of the obtained filtrate was added with 99% methanol to bring the total amount to 500 ml (concentration of methanol). 70%), and the deposited precipitate was filtered, washed with 70% methanol, and then dried. Hereinafter, this polysaccharide is referred to as PS.

As an aqueous acid solution, citric acid (hereinafter referred to as CA), citric acid + K ₂ HPO ₄ (hereinafter referred to as CAK), vitamin C (hereinafter referred to as VC), hydrochloric acid (hereinafter referred to as CL) And used K ₂ HPO ₄ for pH adjustment. By using the PS and CHL obtained here, it was decided to observe the co-growth effect between them by a breeding feed test. Each PS made is P
It was represented as S-CA, PS-CAK, PS-VC, and PS-CL.

[0048]

[Table 3]

The results show that PS alone shows almost no growth and CHL alone slightly increases the body weight. When PS is added to this, it grows well. However, the degree of effect changes depending on the type of acid,
Mineral acid is not good for growth, probably because of its high degree of decomposition. Alkaline salts of organic acids are good, and citric acid is better than vitamin C. In addition to this, the effect was also seen on malic acid and fumaric acid. If you heat only water, CA,
PS, which is as effective as VC, is produced, but decomposition takes time. As can be seen from the drowsiness rate, the growth of silkworms is disparate with PS alone, and even with 7 Hitachi, only a few become 3rd instar.
From this result, it can be seen that there is a co-growth effect between CHL and PS.

Example 2 In Example 1, MLP was used as a raw material for PS, but an attempt was made to investigate the use of other plants and algae as raw materials. The manufacturing method is the same as in Example 1. CHL was also prepared by using MLP as a raw material and treating it in the same manner as in Example 1.

[0051]

[Table 4]

From these results, it was found that the body weight was large even though there were variations in growth rate, the synergistic effect with CHL was recognized, and that many materials other than MLP could be used as PS raw materials as raw materials. It was Sleep rate is almost 85%
As a result, there was no variation in growth.

Example 3 To see the stabilizing effect of VC, a stabilizer (V
C-CHL) was prepared, and the preparation was performed as follows. A mixture of MLP and methanol was extracted and filtered, and 315 ml of the filtrate was mixed with 6.3 g of VC-Na and 135 ml of water, and 500 ml of a mixture having a pH of 7.58 was extracted with petroleum ether. It was created by separating and removing the layers. For comparison, citric acid (C
One using (A) (CA-CHL) and one using only water (water-CHL) were prepared. PS is MLP as a raw material
What was created using was used.

[0054]

[Table 5]

Looking at these results, VC-Na, VC-N
It can be seen that CHL is stabilized by a + K ₂ HPO ₄ and that both the silkworm weight and the drowsiness rate are good, and they all increase in size. V
C is also good, but the stabilizing effect of CA is not so great. V
C-CHL and (VC-Na + K ₂ HPO ₄ ) -CHL show better results than the artificial diet containing MLP as the target.

Example 4 In order to examine the stabilizing effect of polyphenolic acid, a stabilizer with CHL (Phe-CHL) was prepared, which was prepared as follows. 210 ml of a pH 6.33 methanol extract obtained by extracting and filtering a mixture of MLP and methanol
Polyphenolic acid and polyphenol aldehyde were added to and dissolved in 90 ml of water, and 90 ml of water was added thereto to adjust the methanol concentration to 70%, and the precipitate (Phe-CHL) was extracted with petroleum ether to prepare each Phe-CHL.

In order to produce a stable product, the pH was adjusted to 6-8, K ₂ HPO ₄ was added to each to prepare a methanol extract having an appropriate pH, and then water was added. In this case, protocatechuic acid (hereinafter referred to as PAC) had a pH of 7.04, protocatechu aldehyde (hereinafter referred to as PAL) had a pH of 7.54, and gallic acid (hereinafter referred to as GA) had a pH of 6.89. PS used MLP as a raw material.

[0058]

[Table 6]

The results show that chlorophyll stabilized with polyphenolic acid and polyphenolaldehyde was
All of them showed excellent growth effects, and showed the same results as the target artificial diet containing MLP. Furthermore, VC
The effect was enhanced by the combined use with chlorophyll stabilized in (4), and data surpassing the diet containing MLP were also obtained. When both are used in combination, the less Phe-CHL is better.

Example 5 To see the stabilizing effect of sulfur-containing amino acids, a stabilizer with CHL (SA-CHL) was prepared. The creation was performed as follows. 45 ml of a separately prepared amino acid aqueous solution was added to 105 ml of a methanol extract having a pH of 6.24 obtained by extracting and filtering a mixture of MLP and methanol, and the precipitated SA-CHL was extracted with petroleum ether. CH
I made L. The amino acid aqueous solution was prepared as follows. In the case of cysteine, ₂ g of K ₂ HPO ₄ per 1 g of cysteine hydrochloride
In addition, it was dissolved in water to a total volume of 45 ml. In the case of methionine, 1 g of methionine was added with 1 g of K ₂ HPO ₄ and dissolved in water to a total volume of 45 ml. PS used MLP as a raw material.

[0061]

[Table 7]

Looking at these results, cysteine-CHL,
All of methionine-CHL have a growth promoting effect, and the combined use of CHL stabilized with vitamin C further increases the effect.

Example 6 For cherry leaves, Japanese tea leaves, rice leaves, chlorophyll oil, and chlorophyllin-Cu-Na, CHL was extracted in the same manner and its effects were observed. Methanol was added to 15 g of Sakuraba powder and filtered, and 210 ml of the filtrate (pH 6.05) was mixed with 90 g of VC-Na 3 g.
Was dissolved in water and the precipitated CHL was extracted with petroleum ether to obtain CHL. 20 g of Japanese tea leaf powder was extracted with methanol, and 240 ml of the filtrate (pH6.82) was added with VC-Na.
100 ml of a solution (pH 6.60) in which 3 g was dissolved in 90 ml of water was added, and the precipitated CHL was extracted with petroleum ether according to the usual method to obtain CHL.
Got

20 g of rice leaf powder was extracted with methanol, and 135 ml of a solution (pH 7.38) in which 4 g of VC-Na was dissolved in water was added to 315 ml of the filtrate (pH 7.20). Extracted to obtain CHL. Methanol was added to 5 g of chlorophyll oil to filter insoluble matter, and to the filtrate (pH 4.85) of 160 ml was added 68 ml of a solution of VC-Na ₃ g and K ₂ HPO _{4 of} 5 g in water (pH 6.99) for precipitation. CHL was extracted with petroleum ether to obtain CHL. Chlorophylline
Cu-Na 1g was dissolved in methanol 140ml (pH 9.38), and the liquid which dissolved vitamin C 0.7g in 60ml water was added (pH
6.5), the precipitated CHL was taken out with petroleum ether.

[0065]

[Table 8]

From these results, cherry, Japanese tea, rice and chlorophyll oil all showed the effect as CHL, but chlorophyllin had no effect as CHL at all.

Example 7 When the mulberry leaves or artificially reared silkworms reached the 4th age, CHL
When sprayed as a liquid on mulberry leaves, artificial feed or silkworm body,
The silkworm showed the effects of body weight gain and cocoon gain. CHL may be used as a methanol solution as it is, or may be dispersed with an excipient such as glucose or lactose, dissolved in water, and sprayed in a suspended state. The preferred spraying time is the second day for both 4th and 5th instars, and each spray can be effective only once. CHL was dissolved in methanol, and this was sprayed on mulberry leaves and allowed to eat. The result is displayed next.

[0068]

[Table 9] As is clear from the above table, it was found that by simply spraying CHL on mulberry leaves and feeding them, significant weight gain and cocoon growth effects were exhibited and the yield was increased.

[0069]

As described above, according to the silkworm feed of the present invention, chlorophyll, which is a dark oily substance extracted from plant leaves or algae, is a component that promotes the growth of silkworms, and this chlorophyll contains plant leaves and We found that by mixing polysaccharides obtained by extracting seaweed powder such as germ, soybean powder, and seaweed powder with organic acid, a synergistic effect on silkworms was achieved, and without using mulberry or mulberry leaf powder. This has made it possible to breed silkworms.

Further, the above-mentioned chlorophyll and polysaccharides are contained in many plants, algae, etc., and it is possible to select a material cost-effective from the raw materials thereof and easily extract it, so that it is inexpensive. In the conventional mulberry leaf rearing, where the quality of rearing mulberry leaves has been affected by the quality of the mulberry leaves, it is more likely that the cocoons in the early fall silkworm season and the late fall silkworm season will be better than those in the spring silkworm period. It was not possible to increase the income because it was small and it could not be expected to increase it, and the cocoon cost was also decided according to its size, but the chlorophyll that can be preserved was used as an appropriate amount, stabilizer, etc. When used as a mixture of cocoons, it can be constantly provided regardless of the season, efficient breeding can be performed, and good thread quality can be obtained from large cocoons.

In addition, silkworms have been bred for 3 to 4 times a year in accordance with the cultivation of mulberry, which has been bred by mulberry leaves during the sowing period, which has been artificially bred mainly from raw mulberry leaves during the larval stage. However, according to the above-mentioned feed of the present invention, it can be raised year-round regardless of the season, it is possible to take a system of year-round multiple breeding by all age artificial breeding of silkworm, This enables a stable supply of good cocoons.

Claims (5)

[Claims] 1. A chlorophyll obtained from cherry leaves, rice leaves, tea leaves, mulberry leaves, etc., or chlorophyll obtained from algae or commercially available chlorophyll, and seaweed powder such as plant leaves, germ, soybean powder, wakame seaweed, etc. A silkworm feed characterized by comprising a mixture with a polysaccharide obtained by extracting water with an organic acid-containing water. 2. A silkworm feed comprising a mixture of chlorophyll and a polysaccharide to which a stabilizer of chlorophyll is added. 3. The stabilizer is a novel substance obtained by reacting chlorophyll with vitamin C, chlorophyll with polyphenol aldehyde, chlorophyll with polyphenolic acid, and chlorophyll with sulfur-containing amino acid. Silkworm feed as described. 4. Chlorophyll is extracted from plant leaves or algae by using an organic solvent that is freely miscible with water, and water is added to this extract to adjust the solvent concentration to 70-80%. The silkworm feed according to claim 1, which is extracted by separating the water with an organic solvent that is difficult to mix with water. 5. A method for rearing silkworms, which comprises directly spraying a solution obtained by dissolving the chlorophyll in water or an organic solvent onto silkworms or plant leaves.