JP3121036B2 - Plant pest control agent - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant pest control agent, and more particularly to a method for controlling one or more substances selected from edible plants, natural additives and food additives, and acetic acid-containing substances. A plant pest control agent.

[0002]

2. Description of the Related Art Antibacterial activity of plant extracts against plant pathogenic microorganisms has been studied for a long time. For example, 1915 types (Ann. App
l. Biol., 34, 136-143, 1947) and 4
Studies have been conducted on the presence or absence of antibacterial activity of plant extract components of plant species No. 00 (Takeda Research Institute Annual Report, vol. 22, pages 125-132, 1967) against phytopathogenic fungi. It has been found to have antibacterial activity. However, none of the studies has examined whether a mixture of these plant extracts having an antibacterial action and acetic acid has antibacterial properties against plant pathogenic microorganisms, and no studies have been made on their practicality. Absent.

[0003] The presence or absence of an antibacterial effect of 200 kinds of food additives against plant pathogenic bacteria has already been studied (Japanese Society of Plant Pathology Vol. 41, No. 1, 73-7).
6, p. 1975), it has been found that lecithin, iron lactate, tartaric acid, nicotinamide, alginic acid, fumaric acid, sorbic acid, sodium polyphosphate, and sucrose fatty acid ester according to the present invention have an antibacterial effect. . Among them, lecithin has been put to practical use. However, it has not been investigated whether a mixture of these food additives having an antibacterial action and acetic acid has an antibacterial property against plant pathogenic microorganisms, and no study has been made on its practicality.

[0004] On the other hand, it is known that organic acids such as acetic acid have an antibacterial action and are used as antibacterial agents as an active ingredient. In particular, the use of organic acids as food bactericides and bacteriostats has been already practiced in the food field where phytotoxicity is relatively unimportant.

However, in the case of these germicides,
Maintaining a high concentration of organic acids as an active ingredient is an essential condition. However, when organic acids are applied to a plant at such a high concentration as to exhibit a bactericidal action, remarkable phytotoxicity occurs, so that it is impossible to carry out in such a state. Therefore, it is almost impossible to directly spray acetic acid or the like, which exhibits particularly strong phytotoxicity, at a high concentration while maintaining its bactericidal action, on plants with low phytotoxicity.

[0006]

The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, have found that edible plants,
The present inventors have found that a mixture comprising one or more substances selected from natural additives and food additives and acetic acid and acetic acid has antibacterial properties against plant pathogenic microorganisms, and completed the present invention. is there.

[0007] That is, the present invention is (a) bark, mother-in-law
One or two or more edible plants of edible plants selected from cattle, ogre, comfrey, bamboo, nim, calyx , clove, sage, and hops , water of the edible plants or squeezed liquid thereof And / or an extract of an organic solvent or a concentrate thereof, (a) Kakishibu, calcium oxide, saponin, naringin, hesperidin, benzoin
Resin , betaine, pectin degradation product, shirako protein, red
One or more natural additives selected from koji dyes and polylysine , and (u) thiamine thiocyan
Acid salt , L-ascorbic acid, pyridoxine hydrochloride, methyl hesperidin, folic acid, sodium riboflavin 5-phosphate, ferrous gluconate, ferric pyrophosphate,
Sodium benzoate, propylene glycol alginate
, Choline phosphate , glycine, L-alanine, adipic acid, vanillin, piperonal, sodium phytate, sorbitan monolaurate, tannic acid, gallic acid, propylene glycol and lysozyme. One or more substances selected from the group consisting of food additives
One selected from synthetic acetic acid, acetic acid-containing liquid and vinegar
Another object of the present invention is to provide a plant pest control agent comprising two or more acetic acid-containing substances .

[0008] In the component (A) of the present invention, edible plants such as oak, giboshi, ogon, comfrey, ta
One or more plants selected from ke, nim, cinnamon , clove, sage, and hop are used.
Here, as a plant, all parts other than leaves, stems, fruits, and petals can be used. The component (a) means a squeezed liquid of the plant of the above-mentioned edible plant, an extract of the plant itself or a squeezed liquid of water and / or an organic solvent, or a concentrate thereof.

The juice of the plant can be obtained by appropriately crushing or pulverizing the raw edible plant and squeezing the edible plant with, for example, a squeezer or a squeezer.

[0010] The extract of the plant or its juice can be obtained by extracting the plant or its juice with water and / or an organic solvent. Extraction is performed by adding water and / or an organic solvent to a raw or dried edible plant in the form of chips or powder, or squeezed liquid of the edible plant, immersing for an appropriate time, and extracting by filtration. This is done by removing the residue. Here, as the organic solvent, methyl alcohol, ethyl alcohol, propyl alcohol, acetone, ether, acetic acid, ethyl acetate, propylene glycol, glycerin and the like are preferably used. It is preferable to add water and / or an organic solvent in an amount of 5 to 20 parts by weight based on 1 part by weight of the dried plant. Immersion time is 1
0 minutes to 5 hours are preferable, and the higher the extraction temperature, the shorter the extraction time may be. The organic solvent is usually removed from the extract thus extracted, but the organic solvent such as in the case of acetic acid, ethyl alcohol or the like can be used as it is without removing it.

In the present invention, the edible plant squeezed liquid and the extract of the edible plant or its squeezed liquid obtained as described above may be used as a component (a) by a suitable concentration means, for example, heat concentration, It can also be used as a concentrate by concentration under reduced pressure. In addition, the above-mentioned edible plant juice, an extract of the edible plant or its squeezed solution, or a concentrate thereof can be used as the component (A) by mixing one or more kinds. .

Next, in the present invention, as the component (a), oyster, calcium oxide, saponin, naringin, hespe
Lysine, benzoin resin , betaine, pectin degradation product,
One or more natural additives selected from Shirako protein, Monascus dye and polylysine are used.

Furthermore, in the present invention as (c) component, Ji
Amine thiocyanate , L-ascorbic acid, pyridoxine hydrochloride, methyl hesperidin, folic acid, sodium riboflavin 5-phosphate, ferrous gluconate, ferric pyrophosphate, sodium benzoate, pro-alginate
1 selected from pyrene glycol, choline phosphate , glycine, L-alanine, adipic acid, vanillin, piperonal, sodium phytate, sorbitan monolaurate, tannic acid, gallic acid, propylene glycol and lysozyme Seeds or two or more food additives are used.

The control agent of the present invention contains, as an active ingredient, a mixture of one or more substances selected from the above-mentioned components (A) to (C) and a substance containing acetic acid.

Examples of the acetic acid-containing substance used in the present invention include synthetic acetic acid, acetic acid-containing liquid, vinegar and the like. Here, as an example of the acetic acid-containing liquid, there is a vinegar solution described in Japanese Patent No. 950222. This vinegar solution contains acetate in addition to acetic acid, and has the characteristic of causing less phytotoxicity than synthetic acetic acid or vinegar, and is therefore preferred as the acetic acid-containing substance used in the present invention.

Next, the above-mentioned (A) in the controlling agent of the present invention.
The mixing ratio of one or more substances selected from the ingredients (C) and the acetic acid-containing substance is arbitrary, but (A) to (C)
(C) As one or more substances selected from the components, sage , clove, cinnamon, hop, piperona
When using Lumpur, the mixture ratio if to the former 1 part by weight and the latter 0.25 to 4 parts by weight, preferably to express strong synergistic antimicrobial activity against various plant pathogens.

The control agent of the present invention can be applied to diseases of various plants, and is particularly suitable for diseases of turfgrass, vegetables and the like. In particular, turfgrass is highly resistant to the pesticide of the present invention, and can be applied at a relatively high concentration, so that it can be expected to have an excellent effect.

The pesticidal composition of the present invention can be used in the form of powders, granules, etc. by adding a suitable carrier or the like as appropriate in addition to the liquid. The method and concentration of this pesticide depends on the target plant,
Although it varies depending on the type of target disease and other factors, for example, when used as a liquid preparation, the concentration of acetic acid relative to one or more compounds selected from the above (A) to (C) components What is necessary is just to mix | blend so that it may become about 0.002-0.2 weight%, and to spray it to a target plant. The spraying interval is usually set to every 5 to 20 days, and may be determined while observing the control effect.

The pesticidal composition of the present invention does not need to be toxic because it uses components whose safety has already been confirmed. Therefore, the use period can be applied not only to the seedling and growing seasons but also to the harvesting season. This has a very important meaning in complying with the safe use standard of pesticides. For example, if another controlling agent is used during the growing season and the controlling agent of the present invention is used after the harvest period, the amount of the other controlling agent can be reduced.

[0020]

EXAMPLES The present invention will be specifically described below with reference to experimental examples and examples, but the present invention is not limited thereto.

EXPERIMENTAL EXAMPLE 1 Botryotinia fuccegiana IFO 5365 and Botrytis cinerea IFO, which are phytopathogenic fungi, were used as test bacteria.
31831, Cladosporium carpophilum IFO 9645
, Cladosporium fulvum IFO 8419, Fusarium oxysporum IFO 7152, Fusarium roseum formaspecies cerealis IFO9978, Pisium afanidelmatum IFO 7030, Rhizoctonia solani IFO 30464, Screrotinia screoteioram IFO 4876 , Barca Serato Sperma IFO 30252
Were cultivated (preculture) at 25 ° C. for 1 to 5 days on a Petri dish containing 20 ml of PD agar medium (200 g of potato, 20 g of glucose, 15 g of agar, 1 liter of distilled water).

Separately, the edible plants described in Table 1 were placed on a PDS agar medium (supplemented with 2% sucrose on the PD agar medium) supplemented with vinegar so that the acetic acid concentration became 0.02%. An extract, a natural additive or a food additive was added to a predetermined concentration (0.1, 0.3, 1.0% (W / V%)) to prepare a high-pressure sterilizer at 121 ° C. for 15 minutes. Here, the edible plant extract is added with 10 times the amount of water relative to the plant dry powder weight, and extracted on a boiling water bath for 30 minutes,
It refers to the filtered filtrate. In addition, P containing only 0.02% of acetic acid
A DS agar medium (no addition) and a PDS agar medium as a control adjusted to pH 6.0 were prepared and subjected to high-pressure sterilization at 121 ° C. for 15 minutes. Thereafter, each was aseptically dispensed in a sterilized petri dish by 20 ml.

Next, the pre-cultured cells of the test bacteria were inoculated aseptically into the above-mentioned medium with 1 platinum hook and cultured at 25 ° C. for 10 days, and the growth of the bacteria was visually observed. Table 1 shows the results. The numbers in the table indicate the concentration of the additive that inhibited the growth of the test strain in terms of W / V%. In the case of the edible plant extract, it refers to the concentration as a dry powder before extraction. . In addition, + in the table indicates that the growth of the test strain was observed, and-indicates that the effect was not clear because the test could not be performed.

[0024]

[Table 1] Table 1 (Part 1)

[0025]

[Table 2] Table 1 (Part 2)

[0026]

[Table 3] Table 1 (Part 3)

[0027]

[Table 4] Table 1 (Part 4)

[0028]

[Table 5] Table 1 (Part 5)

[0029]

[Table 6] Table 1 (Part 6)

[0030]

[Table 7] Table 1 (Part 7)

[0031]

[Table 8] Table 1 (Part 8)

[0032]

[Table 9] Table 1 (Part 9)

[0033]

[Table 10] Table 1 (Part 10)

[0034]

[Table 11] Table 1 (No. 11)

[0035]

[Table 12] Table 1 (Part 12)

Experimental Example 2 As test bacteria, Agrobacterium rhizogenes A4, which is a plant pathogenic bacterium, and Corynebacterium michiganens
IFO 12471, Ervinia Carotobola Subspecies Carotobola IFO 3380, Pseudomonas syringe pasova lacrimans NIAES 1318, Xanthomonas campestris pasoba oryzae NIAES 1
226, 6 shares of Pseudomonas gourmet ARB 6021007
The cells were cultured (pre-culture) at 30 ° C. for 1 to 3 days on a Petri dish containing 20 ml of D agar medium.

Separately, an edible plant extract, a natural additive or a food additive described in Table 2 was added to a PDS agar medium supplemented with vinegar so that the acetic acid concentration became 0.02% at a predetermined concentration ( (0.1 / 0.3 / 1.0% (W / V%)) was prepared and subjected to high-pressure sterilization at 121 ° C. for 15 minutes. Here, the edible plant extract is added with 10 times the amount of water relative to the plant dry powder weight, and extracted on a boiling water bath for 30 minutes,
It refers to the filtered filtrate. In addition, P containing only 0.02% of acetic acid
DS agar medium and PDS agar medium as a control at pH 6.0
Was prepared and subjected to a high-pressure sterilization treatment at 121 ° C. for 15 minutes. Thereafter, each was aseptically dispensed in a sterilized petri dish by 20 ml.

Next, one platinum loop was aseptically inoculated with one pre-cultured cell of the above-mentioned test bacteria, and then cultured at 30 ° C. for 10 days. Thereafter, the presence or absence of growth of the bacteria was visually observed. Table 2 shows the results. The numbers in the table indicate the concentration of the additive that inhibited the growth of the test strain in terms of W / V%. In the case of the edible plant extract, it refers to the concentration as a dry powder before extraction. . In addition, + in the table indicates that the growth of the test strain was observed, and-indicates that the effect was not clear because the test could not be performed.

[0039]

[Table 13] Table 2 (Part 1)

[0040]

[Table 14] Table 2 (Part 2)

[0041]

[Table 15] Table 2 (Part 3)

[0042]

[Table 16] Table 2 (Part 4)

[0043]

[Table 17] Table 2 (Part 5)

[0044]

[Table 18] Table 2 (Part 6)

[0045]

[Table 19] Table 2 (Part 7)

[0046]

[Table 20] Table 2 (No. 8)

As is clear from Tables 1 and 2, edible plant extracts, natural additives or food additives have the effect of inhibiting the growth of phytopathogenic bacteria in the presence of vinegar.
This supports the effect of the plant pest control agent of the present invention. In addition, the edible plant extract, a natural additive,
Even when vinegar and vinegar were mixed with two or more substances selected from food additives, they did not cancel each other's antibacterial abilities and were effective in controlling the test strain. Table 3 shows examples of main diseases caused by the strains used in this experiment.

[0048]

[Table 21]

EXPERIMENTAL EXAMPLE 3 As a test bacterium, a phytopathogenic fungus strain, Pycium aphanidermatum IFO 7030, was used on a PDS agar medium (potato 20).
0 g, glucose 20 g, sucrose 20 g, distilled water 1 liter)
And cultured at 25 ° C. for 1 to 5 days (preculture).

Separately, piperona was added to PDS agar medium .
Added weight percent Lumpur and acetic acid at a concentration shown in Table 4, was adjusted to pH 5.0, sterilized 15 min at 121 ° C., 20 ml
Each was dispensed into a sterile petri dish.

Then, the pre-cultured cells of the test bacteria were inoculated aseptically into the above-mentioned medium with one platinum hook and cultured at 25 ° C. for 10 days, and the growth of the bacteria was visually observed. Table 4 shows the results. The numbers in the table indicate the concentration of the additive in W / V%. In addition, ++ in the table indicates that the medium developed.
+ Indicates a weakly developed one, and-indicates an undeveloped one.

[0052]

[Table 22] Table 4

Experimental Example 4 As a test microorganism, a plant pathogenic fungus strain, Rhizoctonia solani IFO 30464, was inoculated on a PDS agar medium (200 g of potato, 20 g of glucose, 20 g of sucrose, 1 liter of distilled water).
The cells were cultured at 25 ° C for 1 to 5 days (preculture).

Separately, piperona was added to PDS agar medium .
Lumpur, sage extract or clove extract was added to (extract was obtained in the same manner as in Experimental Example 1.) The weight percent of acetic acid to a concentration shown in Table 5, it was adjusted to pH5.0 , 121
The solution was sterilized at 15 ° C. for 15 minutes, and dispensed in 20 ml portions into a sterile petri dish.

Next, the pre-cultured cells of the test bacteria were inoculated aseptically into the above-mentioned medium with 1 platinum hook and cultured at 25 ° C. for 10 days, and the growth of the bacteria was visually observed. Table 5 shows the results. Also figures in the table showed a concentration of the additive in W / V%
It is . In addition, ++ in the table indicates that the medium developed.
+ Indicates a weakly developed one, and-indicates an undeveloped one.

[0056]

[Table 23] Table 5 (Part 1)

[0057]

[Table 24] Table 5 (Part 2)

[0058]

[Table 25] Table 5 (Part 3)

EXPERIMENTAL EXAMPLE 5 The fungi to be tested were phytopathogenic fungal strains
Screoteioram IFO 4876 was inoculated on a PDS agar medium (potato 200 g, glucose 20 g, sucrose 20 g, distilled water 1 liter) and cultured at 25 ° C. for 1 to 5 days (preculture).

Separately from this, Table 6 shows the weight percentages of piperonal, calyx extract, sage extract or clove extract (the extract was obtained in the same manner as in Experimental Example 1) and acetic acid on a PDS agar medium. PH 5.0.
After that, the mixture was sterilized at 121 ° C. for 15 minutes, and dispensed in a sterilized petri dish in 20 ml portions.

Next, the pre-cultured cells of the test bacteria were inoculated aseptically into the above-mentioned medium with 1 platinum hook and cultured at 25 ° C. for 10 days, and the presence or absence of growth of the bacteria was visually observed. Table 6 shows the results. The numbers in the table indicate the concentration of the additive in W / V%. In addition, ++ in the table indicates that the medium developed.
+ Indicates a weakly developed one, and-indicates an undeveloped one.

[0062]

[Table 26] Table 6 (Part 1)

[0063]

[Table 27] Table 6 (Part 2)

[0064]

[Table 28] Table 6 (Part 3)

[0065]

[Table 29] Table 6 (Part 4)

Experimental Example 6 As a test bacterium, a plant pathogenic bacterial strain, Corynebacterium michiganens IFO12471, was placed on a PDS agar medium (potato
200 g, glucose 20 g, sucrose 20 g, distilled water 1 liter) and cultured at 30 ° C. for 1 day (pre-culture).

Separately, hop extraction was performed on the PDS liquid medium.
The effluent (the extract was obtained in the same manner as in Experimental Example 1) and acetic acid were added so that the concentration by weight of acetic acid became the concentration shown in Table 7, and pH5.
After adjusting to 0, the mixture was sterilized at 121 ° C. for 15 minutes and dispensed into test tubes in 10 ml portions.

Next, 0.05 ml of the pre-cultured solution of the above-mentioned test microorganism was aseptically inoculated into this medium, and cultured with shaking at 30 ° C. for 228 hours. After the culture, the absorbance at 660 nm was measured, and the growth amounts of the test strains were compared relatively. Table 7 shows the results. The numbers in the table shows the concentration of the additive in W / V%, but hot
In the case of extract, the concentration as dry powder before extraction is
U.

[0069]

[Table 30] Table 7

As is clear from the results of Experimental Examples 3 to 6,
When the weight ratio of the edible plant extract, the natural additive or the food additive, and the acetic acid in the vinegar was 1: 0.2 to 4, a strong antibacterial action against the test strain was exhibited.

Example 1 Control effect on turfgrass large patch Preparation of acetic acid-containing solution One liter of brewed vinegar having an acetic acid concentration of 10% by weight was added to 30 eggshells.
g, 14.0 g of sodium bicarbonate and 6.67 g of caustic soda were added and mixed to obtain a liquid containing acetic acid. The acetic acid concentration of this is 5
% By weight.

Preparation of Plant Pest Control Agent 10 parts by weight of pure water was added to 1 part by weight of dry sage powder, extracted on a boiling water bath for 30 minutes, and filtered to obtain a sage extract. 3 parts by weight of the sage extract and 14 parts by weight of the acetic acid-containing liquid obtained in the above were mixed, and a plant pest control agent (A)
I got

[0073] disease control and phytotoxicity test varieties: Zoysia tenuifolia test group: District 1 50m ² chemical spraying: the (2) obtained in plant hazardous 40-fold dilution of the 1990 May 28 days of the biocontrol agent (A) From November 15 of the same year
Sprayed with a shoulder sprayer 17 times every 10 days until day. As a control, a 15-fold dilution of the acetic acid-containing solution obtained in the above was used and sprayed in the same manner as the plant pesticide. Survey: FY lesions number of large patch in two years November 19, was investigated lesion <br/> area and phytotoxicity. Results: shown in Table 8. The control group was observed Usui lesions <br/> diameter of about 80cm, but the onset of a plant pest control agent (A) sprayed plot showed remarkable disease control effect was not observed at all. No phytotoxicity was observed in the application area of the plant pest control agent (A) . In the control plot, slight phytotoxicity was observed. This is considered to be a stress due to continuous application of a high concentration of acetic acid, but it seems that there is no problem in practical use. Thus, it was found that there is a significant effect on the control of turfgrass diseases. The control value in the table was determined by the following equation.

[0074]

(Equation 1)

[0075]

[Table 31] Table 8

Example 2 Test for Control of Cucumber Bacterial Bacterial Disease Preparation of Plant Pest Control Agent 1 part by weight of piperonal and 4 parts by weight of brewed vinegar having an acetic acid concentration of 10% by weight were mixed to obtain a plant pest control agent ( B ). Was.
Next, 10 parts by weight of pure water was added to 1 part by weight of the clove dry powder, extracted on a boiling water bath for 30 minutes, and filtered to obtain a clove extract. 4 parts by weight of the clove extract and 1 part by weight of brewed vinegar having an acetic acid concentration of 10% were mixed to obtain a plant pest control agent ( C ). Further, 1 part by weight of the piperonal, 10 parts by weight of the clove extract and 6 parts by weight of brewed vinegar having an acetic acid concentration of 10% by weight were mixed to obtain a plant pest control agent ( D ).

Disease control and phytotoxicity test Variety: Tokiwa Hikari P3 Cultivation method: house suppression cultivation, sowing on September 3, 1990, planting on October 1, 1990 Test plot: 1 section (80 cm furrow × 40 cm interplant) 1 12 with 6 shares of ward x 2
Chemical spraying: Plant pest control agent obtained in the above ( B )
400-fold dilution of 100-fold dilution of (C), 0.99 of (D)
Double-dilution solution was obtained from October 23, 1990 to November 19, 1990, respectively.
Sprayed with a shoulder sprayer 6 times every 5 days until day. As a control, a 200-fold diluted vinegar with an acetic acid concentration of 10% by weight was used.
Sprayed as above. Investigation: On November 24, 27 and 30, 1990, three times, the number of diseased fruits was investigated with respect to the total number of results. Results: shown in Table 9 . Pest control agents ( B ), ( C ),
( D ) both controlled the occurrence of cucumber rot. In particular, a high control effect was observed with the plant pest control agent ( D ) obtained by mixing piperonal and clove extract with brewed vinegar. The disease could be suppressed by spraying with brewed vinegar alone (control), but slight phytotoxicity was observed in which some leaves and fruits wilted. This is considered to be due to the application of a high concentration of acetic acid. On the other hand, plant pesticides ( B ),
Cucumbers grew quite normally in the ( C ) and ( D ) sprayed plots. The control value in the table was determined by the following equation.

[0078]

(Equation 2)

[0079]

[Table 32] Table 9

Further, in the case where a symptom appears on the fruit as in the present disease, the application of the pesticide before the harvesting season is refrained from the viewpoint of food safety, and as a result, the occurrence of the disease is liable to occur. Become. However, the plant pest control agent of the present invention is a mixture of one or more substances selected from edible plants, natural additives or food additives, and acetic acid-containing substances, whose safety has already been confirmed. Therefore, it is possible to spray until just before the harvest season. Moreover, the components remaining in the crop of the plant pest control agent of the present invention are extremely safe.

[0081]

EFFECT OF THE INVENTION The plant pest control agent of the present invention is used in agricultural and agricultural fields due to the synergistic effect of one or more substances selected from edible plants, natural additives or food additives and acetic acid-containing substances. It is possible to safely and easily control the occurrence of plant diseases on land or green space. Further, the plant pest control agent of the present invention does not cause phytotoxicity. In addition, since the plant pest control agent of the present invention uses components that have already been confirmed to be safe, it can be sprayed until immediately before the harvest season when the safety of foods such as agricultural crops becomes a problem. It is extremely safe even if there are residual components.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI A01N 43/40 101 A01N 43/40 101E 43/78 43/78 A 57/16 104 57/16 104C 59/06 59/06 Z 61/00 61/00 D 63/00 63/00 A D 65/00 65/00 A (56) References JP-A-3-67573 (JP, A) JP-A-62-63505 (JP, A) JP-A-61-233606 (JP, A) JP-A-55-27164 (JP, A) JP-A-54-95725 (JP, A) JP-A-49-81529 (JP, A) JP-A-56-34608 (JP, A) JP, A) JP-A-2-117608 (JP, A) JP-A-60-41473 (JP, A) JP-A-4-95005 (JP, A) Gilliver, Ann. Ap pl. Biol. , 1947, pp. 136-143, Misasa Asami, et al., Journal of the Japanese Society of Plant Pathology, Vol. 41, No. 1, pp. 73-76 (1975) Minoru Goto et al., Annual Report of Takeda Research Institute, Vol. 22, pp. 125-132. (1963) (58) Fields investigated (Int. Cl. ⁷ , DB name) A01N 37/02 Patent file (PATOLIS)

Claims (2)

(57) [Claims] Claims: 1. (a) Oubak, Hosta, Ogon,
Comfrey, bamboo, nim, keihi , chouji, sage
And squeezed liquid of one or more edible plants selected from hops and hops , water and / or organic solvent extracts of the edible plants or the squeezed liquid thereof, or concentrates thereof (a ) Kakishibu, calcium oxide, saponin, nari
Ngin, hesperidin, benzoin resin , betaine, pectin degradation product, white protein, red koji pigment and polyli
One or more natural additives selected from gin and (c) thiamine thiocyanate , L-ascorbic acid, pyridoxine hydrochloride, methyl hesperidin, folic acid, sodium riboflavin 5-phosphate, gluconic acid Ferrous iron, ferric pyrophosphate, sodium benzoate
Propylene glycol alginate, choline phosphate
Salt , glycine, L-alanine, adipic acid, vanillin,
Piperonal, sodium phytate, sorbitan monolaurate, tannic acid, gallic acid, propylene glyco
One or two selected from lysozyme and lysozyme
One selected from the group consisting of more than one food additive
Species or two or more substances, synthetic acetic acid, acetic acid-containing liquid
And one or more vinegars selected from vinegar
A plant pest control agent comprising an acid-containing substance . 2. The plant pest control agent according to claim 1, wherein the target of the plant pest control agent is turfgrass.