JPH0477725B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a cosmetic product characterized by containing a viscous material of white fungus obtained by liquid culture, and more specifically, the present invention relates to a cosmetic product containing a viscous substance of white fungus that is obtained by liquid culture, and more specifically, a viscous substance of white fungus that is excreted from the fungal body by liquid culture of white fungus. The present invention relates to cosmetics containing viscous substances. As water-soluble polymers that have been conventionally used in cosmetics, gum arabic, xanthan gum, sodium alginate, carboxyvinyl polymer, and the like are commonly used. The purposes for which they are used include adjusting the viscosity of the product, providing a smooth feeling of use, and creating a favorable protective film for the skin. However, gum arabic, xanthan gum, etc. have a sticky feel when used, and sodium alginate has the disadvantage that when incorporated into cosmetics, the viscosity decreases over time, degrading the quality of the product. For carboxyvinyl polymers, the viscosity changes depending on the pH, typically PH5.5.
There was a problem that only the range ~8.0 could be used. In view of these circumstances, the present inventors have conducted extensive research to resolve the above-mentioned drawbacks, and have found that cosmetics containing extracts of the fruiting bodies or fungal bodies of wood ear fungi are safe for the skin. It is stable over time, has no PH dependence, and has excellent effects not found in conventional water-soluble polymers, such as a smooth feeling of use, a refreshing aftertaste, and a high skin conditioning effect. We first discovered what could be obtained (patent application (1) filed by our company on May 14, 1985). When blended into cosmetics, the water-extracted components of the fruiting bodies or fungal cells of the white fungus are most suitable;
The cost of raw material for fruiting bodies is high, and the extraction rate from bacterial cells is very low (for example, when hot water is extracted, 50-60% of the extracted components can be obtained from fruiting bodies, but There were practical problems such as only 5 to 10% of extracted components could be obtained. As a result of further intensive research into this problem, the present inventors discovered that the viscous substance discharged outside the bacterial cells by liquid culture of the bacterial cells of the white wood fungus is the above-mentioned fruiting body or hot water extracted component of the bacterial cells of the white wood jellyfish. Similarly, it has been found that when rubbed on the skin, it spreads very smoothly without irritating the skin, and after drying, a very crisp film is obtained that is neither sticky nor bulky. Furthermore, it has been found that excellent skin conditioning effects such as improved skin moisture retention, improved skin color and luster, and prevention and improvement of fine wrinkles can be obtained. That is, the present invention relates to a cosmetic containing a viscous substance that is excreted from the cells of the white fungus through liquid culture of the cells. The cosmetics obtained by the present invention are characterized by improving skin color and luster, providing excellent skin conditioning effects such as preventing and improving fine wrinkles, and leaving behind a protective film with a very refreshing feel. Moreover, it has a high water retention property. In addition, it is a cosmetic that does not irritate the skin and has excellent safety. The viscous substance of white fungus that is blended as an essential ingredient in the present invention is a viscous substance that is excreted outside the white fungus body when the white fungus body is cultured in liquid, and can be easily and easily produced regardless of the season. Can be produced in large quantities. By liquid culturing the white fungus cells of the present invention,
The active ingredients of the viscous substance excreted from the bacterial body are mainly xylose, mannose, glucuronic acid, and acidic polysaccharides having a structure with an acetyl group in these molecules. By liquid culturing the white fungus cells of the present invention,
The viscous substance excreted outside the bacterial cells can be produced as follows. The liquid culture conditions are not particularly limited as long as they allow the white fungus cells to proliferate. Usually, the liquid culture medium includes a carbon source,
A medium containing a nitrogen source and an inorganic salt can be used, and a medium containing natural nutritional sources, vitamins, amino acids, etc. can also be used. Examples of carbon sources include sugars such as sucrose, glucose, fructose, and maltose. Examples of nitrogen sources include peptone, urea, and ammonium salts. Further, examples of inorganic salts include potassium chloride, iron sulfate, magnesium sulfate, phosphates, and the like, and these can be added as necessary. Examples of natural nutritional sources include malt extract, yeast extract, casein hydrolyzate, coconut milk, and potato extract. Culture conditions: temperature 20-32℃, pH
5 to 7, preferably carried out under aerobic conditions by a method such as shaking culture or aerated agitation culture for 5 to 20 days. Although main culture can be performed from the beginning, it is preferable to perform a small-scale preculture in advance and inoculate the main culture with this preculture. The culture solution after liquid culture can be sterilized by a conventional method and used as a raw material for cosmetics. For example, after heating and sterilizing the culture solution after liquid culture, centrifugation or filtration using an agent such as Celite 545 or Hyflo Super Cell, etc.
The liquid obtained by sterilizing the white jellyfish cells, that is, the culture solution containing the viscous substances excreted outside the white jellyfish cells, can be used as a raw material for cosmetics as it is. In addition, the above-mentioned liquid can be further fractionated and purified by methods such as removal of low-molecular components by dialysis, ultrafiltration, etc., protein removal operation, and fractionation using a solvent such as alcohol. .
Further, the culture solution after bacterial cell removal or culture components obtained by further fractionating and purifying the culture can be concentrated or diluted as necessary. Furthermore, it can also be used as a raw material for cosmetics in the form of a dry powder by freeze-drying, spray-drying, or other methods. According to the present invention, the viscous substances (culture solution or culture components after sterilization) discharged from the cells by liquid culture of the cells of White-eared Fungus can be used as lotions, emulsions, lotions, etc., along with various cosmetic base materials. It can be used in the form of basic cosmetics such as creams, makeup cosmetics such as foundations, hair cosmetics such as hair liquids and hair creams, and the like. Moreover, these cosmetics can be manufactured by conventional methods. The appropriate amount of the viscous substance excreted outside the cells by the liquid culture of the cells of the white fungus used in the present invention is 0.001 to 20.0% by weight based on the cosmetic base material. In addition, when using the culture solution without the fungal cells of the white fungus directly, almost 100%
It is also possible to mix. If the blending amount is less than 0.001% by weight, sufficient effects cannot be obtained, and
If the amount exceeds 20.0% by weight, the effect will not be enhanced and it will be uneconomical. Next, the effectiveness of the cosmetics of the present invention will be explained while showing examples. The content of the present invention is as follows:
It is not limited to these. The amounts shown in Examples and Comparative Examples refer to parts by weight. The white jellyfish culture solution and white jellyfish culture components used in the examples were manufactured by the following method.
In both cases, by liquid culture of white fungus cells,
It contains viscous substances that are excreted outside the bacterial body. Example of liquid culture of white fungus by adding purified water to 2 parts of malt extract and 2 parts of sucrose.
After sterilizing 100 parts of the medium (PH5.5), inoculate the white fungus Tremella fuciformis IFO No. 8890.
Shaking culture was carried out at 30°C for 6 days (110 times/min). After the cultivation was completed, the bacterial cells were removed using a centrifuge (12,000 rpm/min), and the resulting supernatant was further filtered through Celite 545 to obtain 80 parts of a liquid, that is, a white fungus culture solution. The above-mentioned white fungus culture solution is subjected to ultrafiltration to remove low-molecular substances with a molecular weight of 8000 or less, and then lyophilized to produce white fungus culture components (yield: 0.1 to 1.0 parts per 100 parts of medium).
I got it. Example 1 Lotion formulation Amount A White fungus culture ingredient 0.6 parts 1,3-butylene glycol 5.0 Glycerin 2.0 Xanthan gum 0.2 Purified water 46.9 B Ethanol 5.0 Methyl p-hydroxybenzoate 0.1 Polyoxyethylene (40) hydrogenated castor oil 0.2 Fragrance Appropriate amount Purified water 10.0 Manufacturing method: Uniformly dissolve component A and component B, then mix to form a product. Comparative Example 1 Lotion A lotion was prepared using the formulation of Example 1 except that the white fungus culture component was removed. Example 2 Skin Cream A Stearic acid 4.0 parts Cetyl alcohol 3.0 Stearyl alcohol 1.0 Liquid paraffin 6.5 Vaseline 10.0 Sorbitan monostearate 1.5 Polyoxyethylene (25) monostearate
3.0 B 1,3-butylene glycol 5.0 Potassium hydroxide 0.1 White fungus culture component 1.0 Methyl p-hydroxybenzoate 0.2 Purified water 64.7 C Fragrance Appropriate amount Production method: Heat oil phase component A and water phase component B to 70-75°C, respectively. After dissolving, component A and component B
Add and emulsify, add component c during cooling, mix, and cool to 30°C to form a product. Example 3 Emulsion A Stearic acid 5.0 parts Cetyl alcohol 5.0 Liquid paraffin 2.0 Glycerin monostearate 1,3 Sorbitan monooleate 1.5 Polyoxyethylene (10) Sorbitan monooleate 0.8 B Glycerin 6.0 White fungus culture solution 6.0 Methyl p-hydroxybenzoate 0.2 Purified water 72.2C Perfume Appropriate amount Manufacturing method: Produce a product in the same manner as in Example 2. Example 4 Pack A Glycerin 15.0 1,3-butylene glycol 10.0 Methyl p-hydroxybenzoate 0.2 Polyoxyethylene (40) Hydrogenated castor oil 0.5 White fungus culture solution 10.0 Citric acid 0.1 Sodium citrate 0.3 Fragrance Appropriate amount of purified water 63.9 Production method: Dissolve each component uniformly to form a product. By liquid culturing the white fungus cells of the present invention,
In order to clarify the safety of the viscous substance discharged outside the bacterial cells, a primary irritation test on the human body was conducted using an occlusion patch test. In other words, using Finch Yanvar (Taisho Pharmaceutical), 30 healthy people were given
An occlusive patch was applied to the flexor side of the forearm for 48 hours, and the average value of the results 1 hour, 24 hours, and 48 hours after removal of the patch test adhesive was used for determination. No erythema or the like was observed in either the white fungus culture solution or the white fungus culture component, and it was confirmed that there was no primary irritation.

[Table] To further clarify the effects of the cosmetics of the present invention
We conducted a usage test on 30 general women between the ages of 25 and 50, and conducted a double-blind questionnaire survey focusing on the skin conditioning effect. Table 2 summarizes the results of using a lotion containing white jellyfish cultured ingredients (the lotion of Example 1) and a conventional lotion that does not contain white jellyfish cultured ingredients (the lotion of Comparative Example 1) for one month. Furthermore, no skin problems occurred during the period of use.

[Table] As is clear from Table 2, excellent skin conditioning effects were obtained by adding the white fungus cultured ingredients. A similar test was conducted using a white fungus culture solution, and an excellent skin conditioning effect was obtained.

Claims (1)

[Claims] 1. A cosmetic containing a viscous substance obtained by liquid culture of white fungus.