JPH0411847A - Feed additive for ruminant - Google Patents

Abstract

PURPOSE:To obtain the title additive capable of protecting a biologically active substance from gastic juice of the first stomach and efficiently absorbing in an alimentary organ after the forth stomach by dispersing the biologically active substance into a specific fatty acid metal salt based protecting matrix. CONSTITUTION:The aimed additive consisting of a pharmaceutical preparation protected by dispersing a biologically active substance (e.g. amino acid, vitamin, protein or animal medicine) into a fatty acid metal salt alone which is a long- chain fatty acid containing 6-10C saturated fatty acid or protecting matrix consisting essentially of the above mentioned fatty acid metal salt.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a feed additive for ruminants, and more particularly, it protects biologically active substances from the gastric juice of the rumen of ruminants. The present invention relates to a preparation in which a biologically active substance is dispersed in a protective matrix consisting solely of a fatty acid metal salt or a fatty acid metal salt as a main component, in order to be efficiently absorbed in the digestive organs starting from the four stomachs.

The feed additive for ruminants of the present invention is added to feed and mixed,
It can be orally administered to ruminants such as cattle and sheep, and is suitably used as a preparation for efficiently absorbing biologically active substances such as amino acids, vitamins, proteins, and veterinary drugs.

[Conventional technology]

When biologically active substances such as amino acids, vitamins, proteins, and veterinary drugs are orally administered to ruminants, they are decomposed by microorganisms present in the gastric juice of the rumen of the ruminant, and are not absorbed as is.

Therefore, in order to efficiently absorb these biologically active substances, the biologically active substances are coated and protected with a substance that is stable against the ruminal fluid, such as oil, and the digestive organs from the abomasum onward are protected. Various feed additives for ruminants that release biologically active substances have been proposed (Japanese Unexamined Patent Publication No. 56-15).
4956, Japanese Unexamined Patent Publication No. 61-151133, etc.), the applicant of the present invention also applied a biologically active substance to hydrogenated oil, etc. for the purpose of imparting disintegrability in the digestive organs from the abomasum onwards. JP-A No. 58-175449 and JP-A-Sho 5 proposed a formulation coated and protected with a protective substance containing chitosan.
9-198946, etc.), and is marketed under the name Rafchit ■.

On the other hand, divalent metals such as calcium and magnesium in fatty acids having 14.16 and/or 18 carbon atoms are used as an energy source that bypasses the rumen of ruminants and is absorbed with high efficiency in the abomasum and subsequent digestive organs. Salt was proposed (US
P 4.826.694), calcium salts of mixed fatty acids made from natural fats and oils (hereinafter referred to as "bypass fats") are commercially available.

In addition, there is a known method for producing feed additives for ruminants, in which these bypass fats and oils are heated to a temperature above their melting point to soften them, a biologically active substance is added and mixed therein, and then the mixture is cooled, solidified, and crushed. (Refer to Japanese Patent Laid-Open No. 63-313546).

[Problem to be solved by the invention]

In feed additives for ruminants in which biologically active substances are coated and protected with the above-mentioned protective substances such as hydrogenated oil, the biologically active substances have ruminal bypass properties and are released in the digestive tract from the abomasum onward. It has excellent storage stability, especially 40
There is a need for improved thermal stability when stored at temperatures above °C.

On the other hand, bypass oils and fats have a higher melting point and excellent thermal stability than hardened oils and the like. Therefore, a preparation in which a biologically active substance is coated and protected with a bypass oil or fat is expected to have excellent thermal stability. However, since bypass fats and oils are usually made from natural fats and oils, there are various types depending on the raw materials used. Therefore, depending on the type of bypass fats and oils used, it may not be possible to obtain sufficient performance of the preparation, and in some cases, it may not work at all. Performance may not be achieved. In particular, the difference is even more remarkable in formulations containing a high content of biologically active substances or formulations containing highly water-soluble biologically active substances.

An object of the present invention is to provide a feed additive for ruminants that has excellent ruminal bypass properties, abomasal release properties, and thermal stability of biologically active substances.

[Means to solve the problem]

In order to achieve the above object, the present invention has conducted intensive research and found that the fatty acid composition of the fatty acid metal salt is The present inventors have discovered that a feed additive for ruminants with excellent ruminal bypass properties and abomasal release properties of biologically active substances can be obtained when the ratio is within a certain range, and the present invention has been completed.

Bypass fats and oils are made from natural fats and oils, and are usually published in Japanese Patent Application Publication No. 1983-1983.
As described in No. 313546, it is produced by saponifying this raw material to form a metal salt, so the fatty acid composition of the bypass oil and fat is approximately the same as that of these raw material oils and fats. These raw material oils and fats are generally palm oil,
Beef tallow and pig intestines are used.

In fact, the bypass oil in USP 4.826.694 is derived from palm oil and beef tallow.
The bypass fat used in the example of No. 3546 is made from beef tallow. These specifications also exemplify other fats and oils as raw material fats and oils. Said USP 4.
The fatty acid composition of the mixed fatty acids used in 826.694 is shown in Table 1, and the fatty acid composition of the oils and fats exemplified as raw material oils and fats in these specifications is shown in Table 2. still,
In the following, caproic acid is C8, caprylic acid C8, capric acid CIO, lauric acid is 01□, myristic acid is C8, palmitic acid is C16, stearic acid is Cps,
Oleic acid is C18°, linoleic acid is 01s, lylunic acid is C1l"', arachidic acid is C20, behenic acid is C22
, lignoceric acid is designated as C21.

The present invention consists of a preparation in which a biologically active substance is dispersed and protected in a fatty acid metal salt alone or in a protective matrix containing a fatty acid metal salt as a main component, and the fatty acid of the fatty acid metal salt has 6 to 10 carbon atoms. This feed additive for ruminants is characterized by being a long-chain fatty acid containing saturated fatty acids.

In the present invention, biologically active substances are substances that are administered to animals and exhibit activities such as promoting fattening, improving milk quality, increasing milk production, preventing diseases, and treating diseases. It is a substance that is easily decomposed in the rumen by microorganisms present in the rumen, and its efficacy is difficult to express as it is.

For example, amino acids: methionine, lysine, driftfan, etc., N-acyl amino acids: N-stearoylmethionine, N-oleylmethionine.

Salts of amino acids, such as calcium salts of N-hydroxymethylmethionine: Hydroxy analogues of amino acids, such as lysine hydrochloride: 2-hydroxy-4-methylmercaptobutyric acid and its calcium salts, etc., Proteins: No powder, casein, potato Protein, soy protein, etc., vitamins: vitamin A, vitamin A acetate, vitamin A palmitate, vitamin D $1 Vitamin E, nicotinic acid and nicotinamide, calcium pantothenate, β-carotene, etc., enzymes: acidic Examples include protease, etc., carbohydrates: glucose, etc., veterinary medicines: antibiotics such as penicillin and tetracycline, anthelmintics such as Negufon, etc., and one or more of these may be used. Various amounts of this biologically active substance are prepared depending on the purpose of administration of the preparation, but if the amount is too low, the amount of feed additive to be fed becomes extremely large, which causes inconvenience. On the other hand, if the amount is too large, the protective matrix will not provide sufficient protection for the biologically active substance, and therefore ruminal bypass property will not be achieved. Therefore, the content of biologically active substances is 2-40% by weight.
is preferable, and more preferably 15 to 30% by weight.

The fatty acid metal salt is a metal salt of a fatty acid, preferably 2
valent metal salts, more preferably calcium salts.

The saturated fatty acid having 6 to 10 carbon atoms of the present invention may have a linear or branched substituent.

The long chain fatty acid is a saturated or unsaturated fatty acid that may have a straight chain or branched substituent having 12 to 24 carbon atoms.

In the present invention, the mixing ratio of saturated fatty acids having 6 to 10 carbon atoms/long chain fatty acids is 1/9 to 515, preferably 2/8 to 4.
/6 (weight ratio), and within this range, the preparation has excellent ruminal bypass properties and elution properties of biologically active substances in the digestive organs from the abomasum onwards.

Furthermore, in order to further improve the rumen bypass property, fatty acids having 10 to 24 carbon atoms, higher alcohols, glycerin fatty acid esters, hardened animal and vegetable oils, wax, etc. can be added.

There is no particular restriction on the proportion of these additions, but it is preferable that the melting point of the protective substance is 60°C or higher,
Particularly preferred is 80°C or higher.

In order to further improve the dissolution of biologically active substances in the abomasum, the formulation of the present invention contains a disintegrating agent that is insoluble in a neutral range and swells, dissolves, or decomposes in an acidic range. can do. An example of such a disintegrating agent is chitosan.

Furthermore, an inorganic filler such as calcium carbonate may be added for the purpose of adjusting the specific gravity of the preparation.

This preparation can be easily produced by mixing the fatty acid metal salt, optionally other additives, and biologically active substances, softening by heating, kneading, and then molding.

An extrusion granulation method or the like can be adopted as a molding method for this preparation, and a conventional extruder for thermoplastic resins or the like is suitably used as the manufacturing device.

〔Example〕

The present invention will be explained in more detail by Examples and Comparative Examples.

However, the scope of the present invention is not limited in any way by the following examples.

In addition, in the following examples, "parts" and "%" are based on weight unless otherwise specified.

(1) Tables 3 and 4 for the preparation of feed additives for rebellious animals
Fatty acid Ca salt and biologically active substance were mixed at the mixing ratio listed in the table, and after kneading at 110 to 160°C for about 2 minutes,
Extrude into a string of ~4 mm, cut into lengths of 3 to 6 mm,
A granular formulation was obtained.

(2) Evaluation test of various preparations For each preparation prepared in the above item (1), 2 g of each was added to Tris buffer corresponding to bovine rumen juice: 20
It was immersed in 0 cc of water and kept shaking at a temperature of 37° C. for 24 hours. The sample was then removed from the Tris buffer at 0.05 M (=m), which corresponds to bovine abomasal juice.

l-dm-j) Hydrochloric acid: It was immersed in 200 cc and kept shaking at a temperature of 37°C for an additional 4 hours. Subsequently, the sample taken out from 0.05M hydrochloric acid was added to a solution corresponding to bovine small intestine fluid + 200c.
The sample was immersed in water and kept shaking at a temperature of 37°C for an additional 4 hours.

Tris buffer Tris [tris(hydroxymethyl)aminomethane]
= 6.06g was dissolved in 0.1M hydrochloric acid of 292-, and diluted with water to 1,0O077+7! The amount of biologically active substance eluted in each of the above corresponding solutions was measured by the method described below.

(a) Lysine: Measured by ninhydrin colorimetric method (b) Methionine: Measured by iodine titration method The results of each measurement are shown in Table 3.

(3) Storage stability test The preparation prepared in the above item (1) was placed in a petri dish and left at room temperature under direct sunlight for 40 days, or at 80°C in the dark for 1 hour.
It was stored for 0 days and the methionine decomposition rate was measured.

The measurement results are shown in Table 4 along with changes in appearance.

〔Effect of the invention〕

As shown in the above examples, the feed additive for ruminants of the present invention has a ruminal bypass property and a rumen bypass property of the biologically active substance contained therein when it is orally administered to ruminants. It has extremely stable and excellent elution characteristics in the digestive tract, and has excellent storage stability.

The present invention provides biologically active substances that are easily degraded in the rumen of ruminants when administered orally, and are suitable for bypassing the rumen and absorbing them with high efficiency in the digestive organs from the abomasum onwards.
Moreover, the present invention provides a feed additive for ruminants that has excellent storage stability, particularly thermal stability, and has extremely great significance in industry, especially in the livestock field.

Claims (1)

[Claims] (1) Consists of a preparation in which a biologically active substance is protected by being dispersed in a protective matrix containing either a fatty acid metal salt alone or a fatty acid metal salt as a main component, and the fatty acid of the fatty acid metal salt is a saturated fatty acid having 6 to 10 carbon atoms. A feed additive for ruminants characterized by being a long-chain fatty acid containing